Molecular Cloning and Ana lysis of Homological Gene PPO from Phalaenopsis

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (12): 1799-1804.

• Ornamental Plants • Previous Articles Next Articles

Molecular Cloning and Ana lysis of Homological Gene PPO from Phalaenopsis

XU Chuan-jun^{1, 2} ; ZHOU Wen-ling¹;CHEN Dong-yin¹; LA I Yan-yan¹; LI Ling^1*

¹Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China; ² Fujian Institute of Subtropical Botany, Xiamen, Fujian 361006, China

Received:2009-05-18 Revised:2009-11-04 Online:2009-12-25 Published:2009-12-25
Contact: LI Ling

Abstract

Abstract: The PPO homolog, designated as PhPPO, was cloned from Phalaenopsis by RT-PCR and RACE. The full length cDNA of PhPPO was 1 851 bp, has an open read frame of 1 647 bp, encoding a protein of 549 amino acids. Bioinformatic analysis showed that PhPPO protein shared the characters of tryosinase family, bearing the conserved CuA and CuB binding domains and a chloroplast-targeting leading peptide.Prokaryotic expression vectors based on pPRoEXHTa had been constructed and then exp ressed in the BL21 (DE3) induced with ITPG.

Key words: Phalaenopsis, PPO, gene, clone, browning, prokaryotic expression

CLC Number:

S 682.31

XU Chuan-jun;;ZHOU Wen-ling;CHEN Dong-yin;LA I Yan-yan;LI Ling. Molecular Cloning and Ana lysis of Homological Gene PPO from Phalaenopsis[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1799-1804.

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Molecular Cloning and Ana lysis of Homological Gene PPO from Phalaenopsis

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