The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (12): 1791-1798.

• Ornamental Plants • Previous Articles Next Articles

The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells

JIANG Chang-hua^{1, 2} ; HU Yong-hong¹ ; XU J ian-yao²;ZHANG Hao² ;SHI Jin-lei² ;LI Min²;WANG Ya-qiong² ; MING Feng^2*

(¹ Shanghai Botanical Garden, Shanghai 200231, China; ² State Key Laboratory of Genetic Engineering, School of Life Science,Fudan University, Shanghai 200433, China)

Received:2009-08-19 Revised:2009-11-27 Online:2009-12-25 Published:2009-12-25

Abstract

Abstract: RcHSP17.8 is a high temperature (HT) induced expressing gene, by RT-PCR and Southern blot indicate that there are several copies of RcHSP17.8 in genome DNA of Rosa hybrida L. RcHSP17.8 is more strongly expressed in varieties resistant to HT, such as‘Schloss Mannieim’, ‘Radio’, ‘Las Vegas’,
‘Caprice de Meiland’, ‘Girl’, meanwhile weakly or not expressed in varieties sensitive to HT, such as‘Kordes’Perfecta’, ‘Hiogi’, ‘Pfalzer Gold’. The results indicate that this gene is possibly relative to the
tolerance in Rosa hybrida L. to HT. In order to study its possible function, RcHSP17.8 gene has been transferred into Escherichia coliBL21. Recombinant RcHSP17.8 was overexpressed in the recombinant E.coli cells which showed imp roved viability under different abiotic stress conditions, such as, high salt concentration,heavymetal and oxidative stress, as compared with control cultures. Those resultswould provide molecular identification principle to selecting varieties of Rosa hybrida L. with strong tolerance to HT.

Key words: Rosa hybrida L., RcHSP1718, induced expression, abiotic stress

CLC Number:

S 685.12

JIANG Chang-hua;;HU Yong-hong;XU J ian-yao;ZHANG Hao;SHI Jin-lei;LI Min;WANG Ya-qiong;MING Feng. The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1791-1798.

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The Expression of RcHSP17.8 Gene Improving Tolerance to Abiotic Stress inRecombinant E.coli Cells

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