Identification and Expressional Analysis of MGT Gene Family in Prunus persica

doi:10.16420/j.issn.0513-353x.2023-0056

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (3): 463-478.doi: 10.16420/j.issn.0513-353x.2023-0056

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Next Articles

Identification and Expressional Analysis of MGT Gene Family in Prunus persica

ZHOU Ping, YAN Shaobin, GUO Rui, JIN Guang^*()

Research Centre for Engineering Technology of Fujian Deciduous Fruits，Fruit Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou 350013，China

Received:2023-03-29 Revised:2023-11-30 Online:2024-03-25 Published:2024-03-22
Contact: JIN Guang

Abstract

Abstract:

To investigate the roles of magnesium transporters（MGTs）in magnesium transportation，the genome-wide identification of peach（Prunus persica）MGTs（PpMGTs）was performed and their effects in exogenous magnesium application on peach tree were studied. A total of eight PpMGTs，which were unevenly distributed in four chromosomes（Chr1，Chr3，Chr6 and Chr8），were identified based on protein homology searches and conserved domain analyses. System evolution analysis results suggested that plant MGTs could be grouped into five different clades which show quantitative differences in species-specific MGTs. PpMGTs had 4-13 exons，ten conserved motifs in protein sequence，and diverse cis-elements of stress response，transcriptional regulation，circadian rhythm，phytohormone response and developmental growth in their upstream promoters. All PpMGTs were transcribed in flowers，fruits，leaves and roots with their unique tissue specific expression pattern. The distinct transcriptional changes of PpMGTs could be observed after exogenous magnesium spraying. Differentially expressed genes found before and after exogenous magnesium application were discrepant in peach leaves and peels，but some of them were commonly involved in the photosynthesis pathway. The transcriptional profile and correlation analysis results suggested that expressional changes of light-harvesting chlorophyll a/b protein complex genes were positively corelated with transcriptional levels of PpMGT4，PpMGT6 and PpMGT8. Heterologous expression of PpMGT4，PpMGT6 and PpMGT8 were able to rescue growth defects of magnesium transport mutant MM281（Salmonella typhimurium mutant），suggesting these three proteins were serving important magnesium transport functions. Thus，the results from sequence characteristics，expressional profiles and exogenous magnesium application showed that PpMGTs had potential functional diversity and synergistic effects in magnesium transport and utilization. The expressional changes of PpMGT4，PpMGT6 and PpMGT8 may associate with exogenous magnesium intake and magnesium utilization.

Key words: Prunus persica, Mg, MGT, gene expression, functional diversity

ZHOU Ping, YAN Shaobin, GUO Rui, JIN Guang. Identification and Expressional Analysis of MGT Gene Family in Prunus persica[J]. Acta Horticulturae Sinica, 2024, 51(3): 463-478.

Figures/Tables 10

Table 1 The properties of PpMGTs

基因 Gene	ID	染色体 Chromosome	氨基酸数 Number of amino acids	分子量/kD MW	等电点 pI	跨膜域 Transmem- brane	亚细胞定位 Subcellular localization
PpMGT1	Prupe.1G027800	Chr1	393	43.71	4.64	2	质膜，叶绿体 Plasma membrane，chloroplast
PpMGT2	Prupe.1G287300	Chr1	433	47.53	4.98	2	高尔基体，细胞质 Golgi，cytoplasm
PpMGT3	Prupe.1G575700	Chr1	455	50.79	5.14	2	叶绿体，线粒体 Chloroplast，mitochondrion
PpMGT4	Prupe.3G100200	Chr3	472	53.39	5.30	1	高尔基体，细胞质 Golgi，cytoplasm
PpMGT5	Prupe.6G116100	Chr6	452	50.71	6.21	2	质膜，叶绿体 Plasma membrane，chloroplast
PpMGT6	Prupe.8G147300	Chr8	491	54.70	4.64	2	叶绿体，细胞质 Chloroplast，cytoplasm
PpMGT7	Prupe.8G147400	Chr8	460	51.20	4.84	2	叶绿体，液泡 Chloroplast，vacuole
PpMGT8	Prupe.8G231400	Chr8	435	48.81	4.54	2	质膜，叶绿体 Plasma membrane，chloroplast

Fig. 1 Multiple protein sequence alignment of the PpMGTs The η symbols refer to 310-helix，and squiggles and arrows indicate α-helices and β-strands. TT letters show β-turns.

Fig. 2 Evolutionary analysis of MGT family members Prunus persica（Pp，），Arabidopsis thaliana（At，），Oryza sativa（Os，），Carica papaya（Cp，），Populus trichocarpa（Pt，），Solanum lycopersicum（Sl，），Vitis vinifera（Vv，），Amborella trichopoda（Amt，），Malus × domestica（Md，），Pyrus communis（Pc，），Prunus mume（Pm，），Rubus occidentalis（Ro，），Fragaria vesca（Fv，），Prunus avium（Pa，），Rosa multiflora （Rm，），Selaginella moellendorffii（Sm，），Chlamydomonas reinhardti（Cr，），and Dunaliella salina（Ds，）.

Fig. 3 Statistics of MGT family members in 18 species based on their reported phylogenetic relationships

Fig. 4 Gene structures（a）and protein motif distributions（b）of MGT families in peach，Arabidopsis，and rice

Fig. 5 Analysis of cis-acting elements at PpMGTs promoters

Fig. 6 Transcriptional expression analysis of PpMGTs in peach tree

Fig. 7 Gene expressional changes（a），photosynthetic rate comparison（b）and metabolic pathway enrichment analysis（c）after magnesium treatment * represents a significant difference between treatment and control group（Student t-test）.

Fig. 8 Expressional changes of Light-harvesting chlorophyll a/b protein complex（LHC）genes（a）and their correlative relations to MGTs transcriptional changes（b） Symbols * or ** indicate that the corresponding genes were differentially expressed in control-treatment comparisons at P < 0.05 or P < 0.01 levels，analyzing by DEseq2.

Fig. 9 Functional verification of PpMGTs in MM281 strain MM1927 is the positive control in experiment.

References 26

[27]	Li J, Yokosho K, Liu S, Cao H R, Yamaji N, Zhu X G, Liao H, Ma J F, Chen Z C. 2020. Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice. Nature Plants, 6 (7):848-859. doi: 10.1038/s41477-020-0686-3 pmid: 32541951
[28]	Li L, Tutone A F, Drummond R S, Gardner R C, Luan S. 2001. A novel family of magnesium transport genes in Arabidopsis. The Plant Cell, 13 (12):2761-2775.
[29]	Liang S, Qi Y, Zhao J, Li Y, Wang R, Shao J, Liu X, An L, Yu F. 2017. Mutations in the Arabidopsis AtMRS2-11/AtMGT10/VAR5 gene cause leaf reticulation. Frontiers in Plant Science,8:2007.
[30]	Liang Z K, Schnable J C. 2018. Functional divergence between subgenomes and gene pairs after whole genome duplications. Molecular Plant, 11 (3):388-397. doi: S1674-2052(17)30380-5 pmid: 29275166
[31]	Liu X M, Hua C X, Liu X D, Riaz M, Liu Y, Dong Z H, Tan Q L, Sun X C, Wu S W, Tan Z H. 2022. Effect of magnesium application on the fruit coloration and sugar accumulation of navel orange(Citrus sinensis Osb.). Scientia Horticulturae, 304 (15):111282.
[32]	Mao D D, Chen J, Tian L, Liu Z, Yang L, Tang R, Li J, Lu C, Yang Y, Shi J. 2014. Arabidopsis transporter MGT6 mediates magnesium uptake and is required for growth under magnesium limitation. The Plant Cell, 26 (5):2234-2248.
[33]	Mao D D, Tian L F, Li L G, Chen J, Deng P Y, Li D P, Luan S. 2008. AtMGT7:an Arabidopsis gene encoding a low-affinity magnesium transporter. Journal of Integrative Plant Biology, 50 (12):1530-1538.
[34]	Meng S F, Zhang B, Tang R J, Zheng X J, Chen R, Liu C G, Jing Y P, Ge H M, Zhang C, Chu Y L, Fu A G, Zhao F G, Luan S, Lan W Z. 2022. Four plasma membrane-localized MGR transporters mediate xylem Mg²⁺ loading for root-to-shoot Mg²⁺ translocation in Arabidopsis. Molecular Plant, 15 (5):805-819.
[35]	Moomaw A S, Maguire M E. 2008. The unique nature of Mg²⁺ channels. Physiology, 23 (5):275-285.
[36]	Nijjar G S. 1985. Nutrition of fruit trees. New Delhi:Kalyani Publishers.
[37]	Palombo I, Daley D O, Rapp M. 2013. Why is the GMN motif conserved in the CorA/Mrs2/Alr1 superfamily of magnesium transport proteins? Biochemistry, 52 (28):4842-4847. doi: 10.1021/bi4007397 pmid: 23781956
[38]	Pam S D, Blaine M, Yves V, Douglas E S. 2015. Polyploidy and genome evolution in plants. Current Opinion in Genetics & Development,35:119-125.
[39]	Pascal B, Sébastien A, Jelle V L, Geert D J, Claire L. 2013. Plant protein interactomes. Annual Review of Plant Biology,64:161-187.
[40]	Reay P F, Fletcher R H, Thomas V. 1998. Chlorophylls,carotenoids and anthocyanin concentrations in the skin of‘Gala’apples during maturation and the influence of foliar applications of nitrogen and magnesium. Journal of the Science of Food and Agriculture, 76 (1):63-71.
[41]	Regon P, Chowra U, Awasthi J P, Borgohain P, Panda S K. 2019. Genome-wide analysis of magnesium transporter genes in Solanum lycopersicum. Computational Biology and Chemistry,80:498-511.
[42]	Saito T, Kobayashi N I, Tanoi K, Iwata N, Suzuki H, Iwata R, Nakanishi T M. 2013. Expression and functional analysis of the CorA-MRS2- ALR-type magnesium transporter family in rice. Plant and Cell Physiology, 54 (10):1673-1683.
[43]	Serrano M, Martínez‐Romero D, Castillo S, Guillén F, Valero D. 2004. Effect of preharvest sprays containing calcium,magnesium and titanium on the quality of peaches and nectarines at harvest and during postharvest storage. Journal of the Science of Food and Agriculture, 84 (11):1270-1276.
[44]	Sponder G, Svidová S, Khan M B, Kolisek M, Schweyen R J, Carugo O, Djinović-Carugo K. 2013. The GMN motif determines ion selectivity in the yeast magnesium channel Mrs2p. Metallomics, 5 (6):745-752.
[45]	Sun Y, Yang R, Li L, Huang J. 2017. The magnesium transporter MGT 10 is essential for chloroplast development and photosynthesis in Arabidopsis thaliana. Molecular Plant, 10 (12):1584-1587. doi: S1674-2052(17)30296-4 pmid: 28989088
[46]	Verde I, Abbott A G, Scalabrin S, Jung S, Shu S, Marroni F, Zhebentyayeva T, Dettori M T, Grimwood J, Cattonaro F. 2013. The high-quality draft genome of peach(Prunus persica)identifies unique patterns of genetic diversity,domestication and genome evolution. Nature Genetics, 45 (5):487-494.
[47]	Wang Y J, Hua X T, Xu J M, Chen Z, Fan T, Zeng Z, Wang H, Hour A L, Yu Q, Ming R. 2019. Comparative genomics revealed the gene evolution and functional divergence of magnesium transporter families in Saccharum. BMC Genomics, 20 (1):1-18.
[1]	Ahmed F, Morsy M. 2001. Response of“Anna”apples trees grown in the new reclamed land to application of some nutrient and ascorbic acid//The Fifth Arabian Horticulture Conference,Ismailia,Egypt.
[2]	Cao H R, Peng W T, Nie M M, Bai S, Chen C Q, Liu Q, Guo Z L, C Liao H, Chen Z C. 2022. Carbon-nitrogen trading in symbiotic nodules depends on magnesium import. Current Biology, 32 (20):4337-4349.
[3]	Carretero-Paulet L, Fares M A. 2012. Evolutionary dynamics and functional specialization of plant paralogs formed by whole and small-scale genome duplications. Molecular Biology & Evolution, 29 (11):3541-3551.
[4]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools:an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202.
[5]	Chen J, Li L G, Liu Z H,Yuan, Y J, Guo L L, Mao D D, Tian L F, Chen L B, Luan S, Li D P. 2009. Magnesium transporter AtMGT 9 is essential for pollen development in Arabidopsis. Cell Research, 19 (7):887-898.
[6]	Chen Z C, Peng W T, Li J, Liao H. 2018. Functional dissection and transport mechanism of magnesium in plants. Seminars in Cell & Developmental Biology,74:142-152.
[7]	Chen Z C, Yamaji N, Horie T, Che J, Li J, An G, Ma J F. 2017. A magnesium transporter OsMGT 1 plays a critical role in salt tolerance in rice. Plant Physiology,174:1837-1849.
[48]	Wang Yong-jun. 2019. The evolution and function of MGT family and transcriptome dynamics of magnesium deficiency in sugarcane[M. D. Dissertation]. Fuzhou: Fujian Agriculture and Forestry University. (in Chinese)
	王勇军. 2019. 甘蔗MGT基因家族鉴定与缺镁胁迫的转录组动力学研究[硕士论文]. 福州: 福建农林大学.
[49]	Waters B M. 2011. Moving magnesium in plant cells. New Phytologist,190:510-513.
[50]	Wen Ting, Li Jing, Zhang Jiaqi, Wei Yi, Zhu Wenrui, Ni Dejiang, Wang Mingle. 2023. Expression and functional identification of magnesium transporter gene CsMGT6in Camellia sinensis. Acta Horticulturae Sinica, 50 (10):2171-2182. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-0506
	文婷, 李婧, 张家琪, 魏仪, 祝文睿, 倪德江, 王明乐. 2023. 树镁转运子基因CsMGT6的表达和功能鉴定. 园艺学报, 50 (10):2171-2182. doi: 10.16420/j.issn.0513-353x.2022-0506
[51]	Xie K, Cakmak I, Wang S, Zhang F, Guo S. 2021. Synergistic and antagonistic interactions between potassium and magnesium in higher plants. The Crop Journal, 9 (2):249-256.
[52]	Xu G X, Guo C C, Shan H Y, Kong H Z. 2012. Divergence of duplicate genes in exon-intron structure. Proceedings of the National Academy of Sciences of the United States of America, 109 (4):1187-1192.
[53]	Xu X F, Wang B, Lou Y, Han W J, Lu J Y, Li D D, Li L G, Zhu J, Yang Z N. 2015. Magnesium Transporter 5 plays an important role in Mg transport for male gametophyte development in Arabidopsis. The Plant Journal, 84 (5):925-936.
[54]	Xu Ying-gang, Zou Zhi, Guo Jing-yuan, Kong Hua, Zhu Guo-peng, Guo An-ping. 2022. Cloning and functional analysis of CpMGT1,a magnesium transporter gene from Carica papaya. Chinese Journal of Tropical Crops, 43 (6):1114-1121. (in Chinese)
	许迎港, 邹智, 郭静远, 孔华, 朱国鹏, 郭安平. 2022. 番木瓜镁离子转运蛋白基因CpMGT1的克隆与功能分析. 热带作物学报, 43 (6):1114-1121. doi: 10.3969/j.issn.1000-2561.2022.06.003
[55]	Yan Y, Mao D, Yang L, Qi J, Zhang X, Tang Q, Li Y, Tang R, Luan S. 2018. Magnesium transporter MGT6 plays an essential role in maintaining magnesium homeostasis and regulating high magnesium tolerance in Arabidopsis. Frontiers in Plant Science,9:274.
[8]	Chen Z C, Yamaji N, Motoyama R, Nagamura Y, Ma J F. 2012. Up-regulation of a magnesium transporter gene OsMGT1is required for conferring aluminum tolerance in rice. Plant Physiology,159:1624-1633.
[9]	Cong Yue-xi, Luo Dong-Feng, Chen Kun-Ming, Jiang Li-xi, Guo Wan-li. 2012. The development of magnesium transport systems in organisms. Journal of Agricultural Biotechnology, 20 (7):837-848. (in Chinese)
	丛悦玺, 骆东峰, 陈坤明, 蒋立希, 郭万里. 2012. 生物镁离子转运体研究进展. 农业生物技术学报, 20 (7):837-848.
[10]	Conn S J, Conn V, Tyerman S D, Kaiser B N, Leigh R A, Gilliham M. 2011. Magnesium transporters,MGT2/MRS2-1 and MGT3/MRS2-5,are important for magnesium partitioning within Arabidopsis thaliana mesophyll vacuoles. New Phytologist, 190 (3):583-594.
[11]	D’Egidio S, Galieni A, Stagnari F, Pagnani G, Pisante M. 2019. Yield,quality and physiological traits of red beet under different magnesium nutrition and light intensity levels. Agronomy, 9 (7):379.
[12]	Deng W, Luo K, Li D, Zheng X, Wei X, Smith W, Thammina C, Lu L, Li Y, Pei Y. 2006. Overexpression of an Arabidopsis magnesium transport gene,AtMGT1,in Nicotiana benthamiana confers Al tolerance. Journal of Experimental Botany, 57 (15):4235-4243. doi: 10.1093/jxb/erl201 pmid: 17101715
[13]	Drummond R S M, Tutone A, Li Y C, Gardner R C. 2006. A putative magnesium transporter AtMRS2- 11 is localized to the plant chloroplast envelope membrane system. Plant Science, 170 (1):78-89.
[14]	Edger P P, McKain M R, Bird K A, van Buren R. 2018. Subgenome assignment in allopolyploids:challenges and future directions. Current Opinion in Plant Biology,42:76-80.
[56]	Zeng L, Zhang Q, Sun R, Kong H, Zhang N, Ma H. 2014. Resolution of deep angiosperm phylogeny using conserved nuclear genes and estimates of early divergence times. Nature Communications, 5 (1):1-12.
[57]	Zhang B, Cakmak I, Feng J, Yu C, Chen X, Xie D, Wu L, Song Z, Cao J, He Y. 2020. Magnesium deficiency reduced the yield and seed germination in wax gourd by affecting the carbohydrate translocation. Frontiers in Plant Science,11:797.
[58]	Zhang L, Wen A, Wu X, Pan X, Wu N, Chen X, Chen Y, Mao D, Chen L, Luan S. 2019a. Molecular identification of the magnesium transport gene family in Brassica napus. Plant Physiology and Biochemistry,136:204-214.
[59]	Zhang L D, Peng Y Y, Li J, Tian X Y, Chen Z C. 2019b. OsMGT1 confers resistance to magnesium defciency by enhancing the import of Mg in rice. International Journal of Molecular Sciences, 20 (1):207.
[60]	Zhao Z, Wang P, Jiao H, Tang C, Liu X, Jing Y, Zhang S, Wu J. 2018. Phylogenetic and expression analysis of the magnesium transporter family in pear,and functional verification of PbrMGT7 in pear pollen. The Journal of Horticultural Science and Biotechnology, 93 (1):51-63.
[61]	Zhou Ping, Lin Zhikai, Guo Rui, Yan Shaobin, Zhang Xiaodan, Ma Xinyi, Jin Guang. 2021. Effects of low temperature treatment on gene expression and flavonoids biosynthesis metabolism in peach(Prunus persica)leaves. Journal of Agricultural Biotechnology, 29 (7):1283-1294. (in Chinese)
	周平, 林志楷, 郭瑞, 颜少宾, 张小丹, 马昕怡, 金光. 2021. 低温处理对桃树叶片基因表达及类黄酮合成代谢的影响. 农业生物技术学报, 29 (7):1283-1294.
[15]	Fougère-Danezan M, Joly S, Bruneau A, Gao X F, Zhang L B. 2015. Phylogeny and biogeography of wild roses with specific attention to polyploids. Annals of Botany, 115 (2):275-291. doi: 10.1093/aob/mcu245 pmid: 25550144
[16]	Gardner R C. 2003. Genes for magnesium transport. Current Opinion in Plant Biology, 6 (3):263-267. pmid: 12753976
[17]	Gebert M, Meschenmoser K, Svidova S, Weghuber J, Schweyen R, Eifler K, Lenz H, Weyand K, Knoop V. 2009. A root-expressed magnesium transporter of the MRS2/MGT gene family in Arabidopsis thaliana allows for growth in low-Mg²⁺ environments. The Plant Cell, 21 (12):4018-4030.
[18]	Geng G T, Cakmak I, Ren T, Lu Z F, Lu J W. 2021. Effect of magnesium fertilization on seed yield,seed quality,carbon assimilation and nutrient uptake of rapeseed plants. Field Crops Research,264:108082.
[19]	He D D, Chen X H, Zhang Y J, Huang Z C, Yin J X, Weng X F, Yang W H, Wu H H, Zhang F S, Wu L Q. 2023. Magnesium is a nutritional tool for the yield and quality of oolong tea(Camellia sinensis L.) and reduces reactive nitrogen loss. Scientia Horticulturae, 308 (27):111590.
[20]	Huang J H, Xu J, Ye X, Luo T Y, Ren L H, Fan G C, Qi Y P, Li Q, Ferrarezi R S, Chen L S. 2019. Magnesium deficiency affects secondary lignification of the vascular system in Citrus sinensis seedlings. Trees, 33 (1):171-182.
[21]	Ishfaq M, Wang Y Q, Yan M W, Wang Z, Wu L Q, Li C J, Li X X. 2022. Physiological essence of magnesium in plants and tts widespread deficiency in the farming system of China. Frontiers in Plant Science,13:802274.
[22]	Jia Yamin, Xu Hao, Hu Wenlang, Wang Yuwen, Ye Xin, Chen Lisong, Li Yan, Guo Jiuxin. 2022. Magnesium deficiency altered in iron absorption,subcellular distribution,and chemical forms in citrus seedlings. Acta Horticulturae Sinica, 49 (5):973-983. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0197
	贾亚敏, 徐浩, 胡文朗, 王玉雯, 叶欣, 陈立松, 李延, 郭九信. 2022. 缺镁对柑橘苗铁的吸收及亚细胞分布和化学形态的影响. 园艺学报, 49 (5):973-983. doi: 10.16420/j.issn.0513-353x.2021-0197
[23]	Jiao Y, Wickett N J, Ayyampalayam S, Chanderbali A S, Landherr L, Ralph P E, Tomsho L P, Hu Y, Liang H, Soltis P S. 2011. Ancestral polyploidy in seed plants and angiosperms. Nature, 473 (7345):97-100.
[24]	Knoop V, Groth-Malonek M, Gebert M, Eifler K, Weyand K. 2005. Transport of magnesium and other divalent cations:evolution of the 2-TM-GxN proteins in the MIT superfamily. Molecular Genetics and Genomics, 274 (3):205-216.
[25]	Kong X, Peng Z, Li D, Ma W, An R, Khan D, Wang X, Liu Y, Yang E, He Y. 2020. Magnesium decreases aluminum accumulation and plays a role in protecting maize from aluminum-induced oxidative stress. Plant and Soil, 457 (1):71-81.
[26]	Li H, Du H, Huang K, Chen X, Liu T, Gao S, Liu H, Tang Q, Rong T, Zhang S. 2016. Identification,and functional and expression analyses of the CorA/MRS2/MGT-type magnesium transporter family in maize. Plant and Cell Physiology, 57 (6):1153-1168.

Identification and Expressional Analysis of MGT Gene Family in Prunus persica

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