Genome-wide Identification and Abiotic Stress Response Analysis of PP2C Family Genes in Rosa chinensis‘Old Blush’

doi:10.16420/j.issn.0513-353x.2022-0752

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (10): 2139-2156.doi: 10.16420/j.issn.0513-353x.2022-0752

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Genome-wide Identification and Abiotic Stress Response Analysis of PP2C Family Genes in Rosa chinensis‘Old Blush’

SHEN Yuxiao¹, ZOU Jinyu¹, LUO Ping², SHANG Wenqian¹, LI Yonghua¹, HE Songlin¹^,³, WANG Zheng¹^,^*(), SHI Liyun¹^,^*()

¹ College of Landscape Architecture and Art，Henan Agricultural University，Zhengzhou 450002，China
² College of Horticulture Science，Zhejiang A & F University，Hangzhou 310000，China
³ College of Horticulture and Landscape Architecture，Henan University of Science and Technology，Xinxiang，Henan 453003，China

Received:2023-05-09 Revised:2023-09-16 Online:2023-10-25 Published:2023-10-30

Abstract

Abstract:

A total of 69 members of the PP2C family were identified from the genome of Rosa chinensis‘Old Blush’using bioinformatics techniques. The majority of the proteins were hydrophilic，and the amino acid lengths ranged from 122 to 1 082 amino acids，with isoelectric points ranging 4.03 and 9.34. Phylogenetic analysis indicated that PP2Cs were divided into 12 subfamily groups，and the genetic structure and conserved motif of the same subfamily had a high similarity. Segmental duplication events were the main reason for the expansion of PP2C family genes. A significant function for the PP2C family of genes in response to stress may be inferred from the prediction of the cis-elements of promoters，which revealed that nearly 85% of PP2Cs had stress response elements and phytohormone response elements. The expression of rose PP2Cs was specific in tissue and developmental stage，and some genes were involved in the response to drought and high-temperature stress. Among them，three members of subgroup A were significantly responsive to drought stress，one member of subgroup A and two members of subgroup G were significantly responsive to high-temperature stress. Using qRT-PCR，the expression patterns of 11 PP2Cs genes under ABA and dehydration treatment were examined. Six subgroup A genes （RC0G0099200，RC1G0458700，RC1G0569200，RC2G0066800，RC2G0089100 and RC5G0571100）and a subgroup G gene（RC3G0083700）showed significantly higher expression levels following two treatments，suggesting that these genes may be activated by ABA and take part in the dehydration response.

Key words: Rosa chinensis, PP2C gene family, abiotic stress, ABA, gene expression

SHEN Yuxiao, ZOU Jinyu, LUO Ping, SHANG Wenqian, LI Yonghua, HE Songlin, WANG Zheng, SHI Liyun. Genome-wide Identification and Abiotic Stress Response Analysis of PP2C Family Genes in Rosa chinensis‘Old Blush’[J]. Acta Horticulturae Sinica, 2023, 50(10): 2139-2156.

Figures/Tables 7

References 43

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基因 ID	上游引物（5′-3′）	下游引物（5′-3′）
Gene ID	Forward primer	Reverse primer
RC0G0099200	TGGGAAATACTGGCAAGGGTC	CACTTCGGATGGCTGCTTTACT
RC1G0458700	GGTCTAGTTGGAGAAAGCG	ATCGGCGGCAATACACTTA
RC1G0478300	AACTCTCCGATCTTCTCACCGT	ATGCCTCTGACTCTGAAACCTTGC
RC1G0569200	AGGATGCTGGCAATTCTGT	TACTCCCGTCCCTCGTTTCA
RC2G0066800	AAGTGATGGACTGTGGGATGT	CTGACGTTTTCTTTTCGTTTTGA
RC2G0089100	ATGATGAGGGTGAGATTTGC	CCTTCCCATTGGAGCTTGTC
RC3G0083700	CTGATGTTAAACCAGCGGA	GTCCAATGTCAGCGAAGC
RC3G0340800	CTCCGAACTTCTGGAACTGT	ATCATATCTTGTTGGCACCC
RC5G0571100	AAGAGAAGGGTAATCTGGTCAAA	TCCGAACTTGGCGTCAGG
RC6G0593000	GCGGTGCCATTTAGAGTAGGT	CCCTGAATGACTACAATTACAATCC
RC7G0167900	CCGAGATTATGATGTCCCAAGAA	GTCGTAGACGGCGAAGAAGTG
RcGAPDH	TGAAGGGTGGTGCCAAGAA	AAGGGGAGCAAGACAGTTGG

基因 ID	上游引物（5′-3′）	下游引物（5′-3′）
Gene ID	Forward primer	Reverse primer
RC0G0099200	TGGGAAATACTGGCAAGGGTC	CACTTCGGATGGCTGCTTTACT
RC1G0458700	GGTCTAGTTGGAGAAAGCG	ATCGGCGGCAATACACTTA
RC1G0478300	AACTCTCCGATCTTCTCACCGT	ATGCCTCTGACTCTGAAACCTTGC
RC1G0569200	AGGATGCTGGCAATTCTGT	TACTCCCGTCCCTCGTTTCA
RC2G0066800	AAGTGATGGACTGTGGGATGT	CTGACGTTTTCTTTTCGTTTTGA
RC2G0089100	ATGATGAGGGTGAGATTTGC	CCTTCCCATTGGAGCTTGTC
RC3G0083700	CTGATGTTAAACCAGCGGA	GTCCAATGTCAGCGAAGC
RC3G0340800	CTCCGAACTTCTGGAACTGT	ATCATATCTTGTTGGCACCC
RC5G0571100	AAGAGAAGGGTAATCTGGTCAAA	TCCGAACTTGGCGTCAGG
RC6G0593000	GCGGTGCCATTTAGAGTAGGT	CCCTGAATGACTACAATTACAATCC
RC7G0167900	CCGAGATTATGATGTCCCAAGAA	GTCGTAGACGGCGAAGAAGTG
RcGAPDH	TGAAGGGTGGTGCCAAGAA	AAGGGGAGCAAGACAGTTGG

亚族 Sub- group	基因ID Gene ID	染色体位置 Chromosome location	编码区长/bp CDS length	氨基酸数 Amino acid	外显子数 No. of exon	分子量 Molecular weight	等电点 pI	亲水系数 GRAVY	亚细胞定位 Subcellular localization
A	RC0G0047200	Chr00：4828630 ~ 4831238	699	232	4	25 695.51	5.53	-0.031	线粒体内膜Mitochondrial inner membrane
	RC0G0099200	Chr00：11758820 ~ 11762242	1 659	552	4	59 394.42	4.96	-0.063	质膜Plasma membrane
	RC1G0458700	Chr01：55057837 ~ 55059388	1 236	411	3	44 724.48	5.59	-0.377	细胞核Nucleus
	RC1G0569200	Chr01：62821746 ~ 62824036	1 161	386	3	42 500.89	6.98	-0.380	细胞核Nucleus
	RC2G0066800	Chr02：5299187 ~ 5304192	1 167	388	4	42 372.51	5.11	-0.344	质膜Plasma membrane
	RC2G0089100	Chr02：6920939 ~ 6922931	1 431	476	4	52 094.86	5.55	-0.336	质膜Plasma membrane
	RC5G0377600	Chr05：37177437 ~ 37179960	1 236	411	5	46 446.77	7.89	-0.403	质膜Plasma membrane
	RC5G0571100	Chr05：66869860 ~ 66872143	1 203	400	4	43 414.04	5.46	-0.310	细胞核Nucleus
	RC6G0593000	Chr06：66284925 ~ 66287701	1 656	551	4	59 602.25	4.60	-0.138	质膜Plasma membrane
	RC7G0167900	Chr07：13015997 ~ 13016725	465	154	1	16 745.85	6.06	-0.321	细胞质Cytoplasm
	RC7G0168100	Chr07：13018177 ~ 13019011	450	149	2	15 981.51	6.08	0.281	细胞质Cytoplasm
B	RC1G0478300	Chr01：56571375 ~ 56574342	1 143	380	4	41 067.86	8.18	-0.255	细胞核Nucleus
	RC4G0375700	Chr04：49184893 ~ 49188051	1 740	579	5	63 097.54	8.81	-0.546	细胞核Nucleus
	RC5G0606100	Chr05：70444924 ~ 70446964	1 149	382	4	41 507.95	7.53	-0.358	细胞核Nucleus
	RC6G0015800	Chr06：1590043 ~ 1592204	1 323	440	5	49 069.04	6.99	-0.331	细胞核Nucleus
C	RC3G0258700	Chr03：34531274 ~ 34531798	525	174	1	19 636.09	4.42	-0.137	细胞质Cytoplasm
	RC3G0259000	Chr03：34572348 ~ 34575352	2 046	681	4	76 180.77	5.67	-0.499	细胞核Nucleus
	RC4G0275900	Chr04：39562228 ~ 39565705	1 785	594	4	64 995.55	4.92	-0.125	质膜Plasma membrane
	RC5G0553600	Chr05：64738021 ~ 64741007	2 208	735	4	81 714.31	5.43	-0.486	细胞质Cytoplasm
	RC7G0290700	Chr07：26235143 ~ 26240816	2 301	766	5	83 960.48	5.27	-0.542	细胞核Nucleus
D	RC1G0426500	Chr01：52335182 ~ 52338968	1 155	384	4	42 742.27	8.82	-0.172	质膜Plasma membrane
	RC2G0128300	Chr02：10555792 ~ 10559454	1 182	393	4	43 613.79	8.56	-0.223	质膜Plasma membrane
	RC2G0207300	Chr02：18414833 ~ 18417905	1 119	372	4	41 544.15	5.91	-0.258	细胞核Nucleus
	RC3G0144700	Chr03：22626525 ~ 22629894	1 149	382	4	43 154.29	6.43	-0.222	线粒体基质Mitochondrial matrix space
	RC3G0245900	Chr03：33482641 ~ 33485442	1 143	380	4	42 790.86	9.34	-0.362	细胞核Nucleus
	RC4G0305900	Chr04：43009598 ~ 43013252	1 155	384	4	42 277.11	6.31	-0.182	细胞核Nucleus
	RC6G0083700	Chr06：8901670 ~ 8903762	1 149	382	4	42 629.68	8.78	-0.288	细胞核Nucleus
	RC6G0295500	Chr06：39208249 ~ 39213326	1 188	395	4	43 907.85	8.45	-0.291	细胞核Nucleus
E	RC2G0570600	Chr02：65118745 ~ 65121601	1 413	470	5	52 006.45	5.45	-0.488	细胞核Nucleus
	RC3G0300800	Chr03：37901846 ~ 37903908	1 359	452	5	50 180.95	4.92	-0.529	细胞质Cytoplasm
	RC3G0340800	Chr03：41156572 ~ 41159167	1 125	374	5	41 586.10	5.84	-0.343	细胞核Nucleus
	RC3G0415800	Chr03：46406403 ~ 46410803	1 500	499	5	54 901.47	5.28	-0.451	叶绿体基质Chloroplast stroma
	RC4G0174800	Chr04：25897436 ~ 25902101	1 233	410	5	44 205.25	7.96	-0.160	细胞核Nucleus
	RC4G0290900	Chr04：41153285 ~ 41155207	1 164	387	5	42 900.06	8.19	-0.217	内质网Endoplasmic reticulum
	RC5G0637500	Chr05：74500818-74504788	1 602	533	5	58 727.27	5.24	-0.404	叶绿体基质Chloroplast stroma
	RC6G0585500	Chr06：65787874 ~ 65789999	1 014	337	4	36 815.15	4.67	-0.238	细胞质Cytoplasm
F	RC1G0561000	Chr01：62166916 ~ 62170959	1 050	349	8	38 095.69	5.30	-0.349	线粒体基质Mitochondrial matrix space
	RC5G0161600	Chr05：12241573 ~ 12242147	384	127	2	13 699.74	6.26	0.189	细胞质Cytoplasm
	RC5G0287900	Chr05：25199030 ~ 25202031	849	282	5	31 280.45	6.07	-0.424	细胞质Cytoplasm
	RC6G0448200	Chr06：55396868 ~ 55401178	879	292	8	31 419.82	5.04	-0.334	细胞质Cytoplasm
	RC7G0553900	Chr07：63513147 ~ 63518291	849	282	5	31 005.94	7.73	-0.491	线粒体基质Mitochondrial matrix space
G	RC2G0115900	Chr02：9573649 ~ 9576363	1 260	419	4	45 563.01	6.43	-0.214	细胞核Nucleus
	RC2G0023300	Chr02：1683170 ~ 1685525	1 038	345	4	38 186.16	5.23	-0.292	细胞质Cytoplasm
	RC3G0083700	Chr03：14633161 ~ 14635520	1 176	391	4	43 297.18	4.93	-0.175	过氧化物酶体Peroxisome
	RC4G0312700	Chr04：43671766 ~ 43674255	1 140	379	4	42 030.04	5.33	-0.015	内质网Endoplasmic reticulum
	RC6G0313800	Chr06：41654079 ~ 41656687	1 158	385	4	41 854.12	5.25	-0.280	细胞质Cytoplasm
	RC6G0447100	Chr06：55271914 ~ 55274566	1 134	377	4	41 279.55	5.40	-0.315	细胞质Cytoplasm
	RC7G0347300	Chr07：33066169 ~ 33069400	1 089	362	4	39 359.71	5.58	-0.226	细胞质Cytoplasm
H	RC3G0339900	Chr03：41087943 ~ 41091127	1 308	435	7	46 914.75	6.20	-0.114	内质网Endoplasmic reticulum
	RC4G0389000	Chr04：50185684 ~ 50188564	1 293	430	9	46 157.67	6.23	-0.137	细胞质Cytoplasm
	RC6G0560400	Chr06：63913434 ~ 63917402	1 293	430	9	46 572.74	5.06	-0.319	质膜Plasma membrane
I	RC2G0409900	Chr02：47147013 ~ 47154068	1 212	403	11	44 511.31	5.32	-0.302	内质网Endoplasmic reticulum
	RC6G0451300	Chr06：55557811 ~ 55562884	1 152	383	10	42 008.38	4.59	-0.225	质膜Plasma membrane
J	RC3G0144900	Chr03：22635737 ~ 22638365	1 434	477	7	52 805.38	5.43	-0.232	内质网Endoplasmic reticulum
	RC6G0396700	Chr06：50768308 ~ 50778537	3 144	1 047	22	118 157.21	5.74	-0.345	细胞质Cytoplasm
K	RC2G0161300	Chr02：13346491 ~ 13350340	1 320	439	4	46 451.30	7.64	-0.095	细胞质Cytoplasm
	RC5G0146400	Chr05：10980909 ~ 10981772	864	287	1	31 988.92	5.48	-0.538	细胞核Nucleus
	RC5G0168100	Chr05：12965254 ~ 12966114	861	286	1	31 063.42	5.33	-0.106	细胞质Cytoplasm
	RC5G0616200	Chr05：71710833 ~ 71713396	915	304	11	32 791.37	5.51	0.045	细胞核Nucleus
	RC6G0481800	Chr06：58085355 ~ 58088427	1 512	503	4	53 601.63	6.07	-0.277	线粒体基质Mitochondrial matrix space
	RC7G0438400	Chr07：47207142 ~ 47214206	1 866	621	7	66 851.18	4.77	-0.624	细胞质Cytoplasm
L	RC2G0063500	Chr02：4938066 ~ 4945505	3 249	1 082	15	119 968.55	5.10	-0.268	叶绿体类囊体膜Chloroplast thylakoid membrane
	RC4G0257100	Chr04：37263358 ~ 37266748	1 281	426	3	46 285.35	5.08	-0.413	叶绿体基质Chloroplast stroma
Others	RC1G0573600	Chr01：63257965 ~ 63261116	1 200	399	11	43 032.47	5.24	-0.136	过氧化物酶体Peroxisome
	RC4G0054600	Chr04：5904928 ~ 5907032	840	279	5	31 486.20	9.00	-0.428	过氧化物酶体Peroxisome
	RC4G0234300	Chr04：34375420 ~ 34381498	1 707	568	13	62 714.19	5.46	-0.268	内质网Endoplasmic reticulum
	RC5G0408300	Chr05：4088239 ~ 40883657	369	122	3	13 395.99	4.03	0.011	细胞质Cytoplasm
	RC6G0347300	Chr06：45436974 ~ 45440004	1 977	658	1	72 871.19	5.71	-0.167	过氧化物酶体Peroxisome
	RC7G0024700	Chr07：1516095 ~ 1519053	999	332	10	36 056.93	7.69	-0.269	细胞核Nucleus

亚族 Sub- group	基因ID Gene ID	染色体位置 Chromosome location	编码区长/bp CDS length	氨基酸数 Amino acid	外显子数 No. of exon	分子量 Molecular weight	等电点 pI	亲水系数 GRAVY	亚细胞定位 Subcellular localization
A	RC0G0047200	Chr00：4828630 ~ 4831238	699	232	4	25 695.51	5.53	-0.031	线粒体内膜Mitochondrial inner membrane
	RC0G0099200	Chr00：11758820 ~ 11762242	1 659	552	4	59 394.42	4.96	-0.063	质膜Plasma membrane
	RC1G0458700	Chr01：55057837 ~ 55059388	1 236	411	3	44 724.48	5.59	-0.377	细胞核Nucleus
	RC1G0569200	Chr01：62821746 ~ 62824036	1 161	386	3	42 500.89	6.98	-0.380	细胞核Nucleus
	RC2G0066800	Chr02：5299187 ~ 5304192	1 167	388	4	42 372.51	5.11	-0.344	质膜Plasma membrane
	RC2G0089100	Chr02：6920939 ~ 6922931	1 431	476	4	52 094.86	5.55	-0.336	质膜Plasma membrane
	RC5G0377600	Chr05：37177437 ~ 37179960	1 236	411	5	46 446.77	7.89	-0.403	质膜Plasma membrane
	RC5G0571100	Chr05：66869860 ~ 66872143	1 203	400	4	43 414.04	5.46	-0.310	细胞核Nucleus
	RC6G0593000	Chr06：66284925 ~ 66287701	1 656	551	4	59 602.25	4.60	-0.138	质膜Plasma membrane
	RC7G0167900	Chr07：13015997 ~ 13016725	465	154	1	16 745.85	6.06	-0.321	细胞质Cytoplasm
	RC7G0168100	Chr07：13018177 ~ 13019011	450	149	2	15 981.51	6.08	0.281	细胞质Cytoplasm
B	RC1G0478300	Chr01：56571375 ~ 56574342	1 143	380	4	41 067.86	8.18	-0.255	细胞核Nucleus
	RC4G0375700	Chr04：49184893 ~ 49188051	1 740	579	5	63 097.54	8.81	-0.546	细胞核Nucleus
	RC5G0606100	Chr05：70444924 ~ 70446964	1 149	382	4	41 507.95	7.53	-0.358	细胞核Nucleus
	RC6G0015800	Chr06：1590043 ~ 1592204	1 323	440	5	49 069.04	6.99	-0.331	细胞核Nucleus
C	RC3G0258700	Chr03：34531274 ~ 34531798	525	174	1	19 636.09	4.42	-0.137	细胞质Cytoplasm
	RC3G0259000	Chr03：34572348 ~ 34575352	2 046	681	4	76 180.77	5.67	-0.499	细胞核Nucleus
	RC4G0275900	Chr04：39562228 ~ 39565705	1 785	594	4	64 995.55	4.92	-0.125	质膜Plasma membrane
	RC5G0553600	Chr05：64738021 ~ 64741007	2 208	735	4	81 714.31	5.43	-0.486	细胞质Cytoplasm
	RC7G0290700	Chr07：26235143 ~ 26240816	2 301	766	5	83 960.48	5.27	-0.542	细胞核Nucleus
D	RC1G0426500	Chr01：52335182 ~ 52338968	1 155	384	4	42 742.27	8.82	-0.172	质膜Plasma membrane
	RC2G0128300	Chr02：10555792 ~ 10559454	1 182	393	4	43 613.79	8.56	-0.223	质膜Plasma membrane
	RC2G0207300	Chr02：18414833 ~ 18417905	1 119	372	4	41 544.15	5.91	-0.258	细胞核Nucleus
	RC3G0144700	Chr03：22626525 ~ 22629894	1 149	382	4	43 154.29	6.43	-0.222	线粒体基质Mitochondrial matrix space
	RC3G0245900	Chr03：33482641 ~ 33485442	1 143	380	4	42 790.86	9.34	-0.362	细胞核Nucleus
	RC4G0305900	Chr04：43009598 ~ 43013252	1 155	384	4	42 277.11	6.31	-0.182	细胞核Nucleus
	RC6G0083700	Chr06：8901670 ~ 8903762	1 149	382	4	42 629.68	8.78	-0.288	细胞核Nucleus
	RC6G0295500	Chr06：39208249 ~ 39213326	1 188	395	4	43 907.85	8.45	-0.291	细胞核Nucleus
E	RC2G0570600	Chr02：65118745 ~ 65121601	1 413	470	5	52 006.45	5.45	-0.488	细胞核Nucleus
	RC3G0300800	Chr03：37901846 ~ 37903908	1 359	452	5	50 180.95	4.92	-0.529	细胞质Cytoplasm
	RC3G0340800	Chr03：41156572 ~ 41159167	1 125	374	5	41 586.10	5.84	-0.343	细胞核Nucleus
	RC3G0415800	Chr03：46406403 ~ 46410803	1 500	499	5	54 901.47	5.28	-0.451	叶绿体基质Chloroplast stroma
	RC4G0174800	Chr04：25897436 ~ 25902101	1 233	410	5	44 205.25	7.96	-0.160	细胞核Nucleus
	RC4G0290900	Chr04：41153285 ~ 41155207	1 164	387	5	42 900.06	8.19	-0.217	内质网Endoplasmic reticulum
	RC5G0637500	Chr05：74500818-74504788	1 602	533	5	58 727.27	5.24	-0.404	叶绿体基质Chloroplast stroma
	RC6G0585500	Chr06：65787874 ~ 65789999	1 014	337	4	36 815.15	4.67	-0.238	细胞质Cytoplasm
F	RC1G0561000	Chr01：62166916 ~ 62170959	1 050	349	8	38 095.69	5.30	-0.349	线粒体基质Mitochondrial matrix space
	RC5G0161600	Chr05：12241573 ~ 12242147	384	127	2	13 699.74	6.26	0.189	细胞质Cytoplasm
	RC5G0287900	Chr05：25199030 ~ 25202031	849	282	5	31 280.45	6.07	-0.424	细胞质Cytoplasm
	RC6G0448200	Chr06：55396868 ~ 55401178	879	292	8	31 419.82	5.04	-0.334	细胞质Cytoplasm
	RC7G0553900	Chr07：63513147 ~ 63518291	849	282	5	31 005.94	7.73	-0.491	线粒体基质Mitochondrial matrix space
G	RC2G0115900	Chr02：9573649 ~ 9576363	1 260	419	4	45 563.01	6.43	-0.214	细胞核Nucleus
	RC2G0023300	Chr02：1683170 ~ 1685525	1 038	345	4	38 186.16	5.23	-0.292	细胞质Cytoplasm
	RC3G0083700	Chr03：14633161 ~ 14635520	1 176	391	4	43 297.18	4.93	-0.175	过氧化物酶体Peroxisome
	RC4G0312700	Chr04：43671766 ~ 43674255	1 140	379	4	42 030.04	5.33	-0.015	内质网Endoplasmic reticulum
	RC6G0313800	Chr06：41654079 ~ 41656687	1 158	385	4	41 854.12	5.25	-0.280	细胞质Cytoplasm
	RC6G0447100	Chr06：55271914 ~ 55274566	1 134	377	4	41 279.55	5.40	-0.315	细胞质Cytoplasm
	RC7G0347300	Chr07：33066169 ~ 33069400	1 089	362	4	39 359.71	5.58	-0.226	细胞质Cytoplasm
H	RC3G0339900	Chr03：41087943 ~ 41091127	1 308	435	7	46 914.75	6.20	-0.114	内质网Endoplasmic reticulum
	RC4G0389000	Chr04：50185684 ~ 50188564	1 293	430	9	46 157.67	6.23	-0.137	细胞质Cytoplasm
	RC6G0560400	Chr06：63913434 ~ 63917402	1 293	430	9	46 572.74	5.06	-0.319	质膜Plasma membrane
I	RC2G0409900	Chr02：47147013 ~ 47154068	1 212	403	11	44 511.31	5.32	-0.302	内质网Endoplasmic reticulum
	RC6G0451300	Chr06：55557811 ~ 55562884	1 152	383	10	42 008.38	4.59	-0.225	质膜Plasma membrane
J	RC3G0144900	Chr03：22635737 ~ 22638365	1 434	477	7	52 805.38	5.43	-0.232	内质网Endoplasmic reticulum
	RC6G0396700	Chr06：50768308 ~ 50778537	3 144	1 047	22	118 157.21	5.74	-0.345	细胞质Cytoplasm
K	RC2G0161300	Chr02：13346491 ~ 13350340	1 320	439	4	46 451.30	7.64	-0.095	细胞质Cytoplasm
	RC5G0146400	Chr05：10980909 ~ 10981772	864	287	1	31 988.92	5.48	-0.538	细胞核Nucleus
	RC5G0168100	Chr05：12965254 ~ 12966114	861	286	1	31 063.42	5.33	-0.106	细胞质Cytoplasm
	RC5G0616200	Chr05：71710833 ~ 71713396	915	304	11	32 791.37	5.51	0.045	细胞核Nucleus
	RC6G0481800	Chr06：58085355 ~ 58088427	1 512	503	4	53 601.63	6.07	-0.277	线粒体基质Mitochondrial matrix space
	RC7G0438400	Chr07：47207142 ~ 47214206	1 866	621	7	66 851.18	4.77	-0.624	细胞质Cytoplasm
L	RC2G0063500	Chr02：4938066 ~ 4945505	3 249	1 082	15	119 968.55	5.10	-0.268	叶绿体类囊体膜Chloroplast thylakoid membrane
	RC4G0257100	Chr04：37263358 ~ 37266748	1 281	426	3	46 285.35	5.08	-0.413	叶绿体基质Chloroplast stroma
Others	RC1G0573600	Chr01：63257965 ~ 63261116	1 200	399	11	43 032.47	5.24	-0.136	过氧化物酶体Peroxisome
	RC4G0054600	Chr04：5904928 ~ 5907032	840	279	5	31 486.20	9.00	-0.428	过氧化物酶体Peroxisome
	RC4G0234300	Chr04：34375420 ~ 34381498	1 707	568	13	62 714.19	5.46	-0.268	内质网Endoplasmic reticulum
	RC5G0408300	Chr05：4088239 ~ 40883657	369	122	3	13 395.99	4.03	0.011	细胞质Cytoplasm
	RC6G0347300	Chr06：45436974 ~ 45440004	1 977	658	1	72 871.19	5.71	-0.167	过氧化物酶体Peroxisome
	RC7G0024700	Chr07：1516095 ~ 1519053	999	332	10	36 056.93	7.69	-0.269	细胞核Nucleus

Genome-wide Identification and Abiotic Stress Response Analysis of PP2C Family Genes in Rosa chinensis‘Old Blush’

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