Magnesium Deficiency Altered in Iron Absorption,Subcellular Distribution,and Chemical Forms in Citrus Seedlings

doi:10.16420/j.issn.0513-353x.2021-0197

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 973-983.doi: 10.16420/j.issn.0513-353x.2021-0197

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Magnesium Deficiency Altered in Iron Absorption,Subcellular Distribution,and Chemical Forms in Citrus Seedlings

JIA Yamin¹, XU Hao¹, HU Wenlang¹, WANG Yuwen¹^,², YE Xin¹, CHEN Lisong¹, LI Yan¹, GUO Jiuxin¹^,²^,^*()

¹Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation,College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China
²International Magnesium Institute,College of Resources and Environment,Agriculture and Forestry University,Fuzhou 350002,China

Received:2021-11-23 Revised:2022-01-12 Online:2022-05-25 Published:2022-05-25
Contact: GUO Jiuxin E-mail:jiuxinguo@hotmail.com

Abstract

Abstract:

Citrus is mainly grown in acid red soil region of southern China,which has Mg deficiency and rich in Fe. The aim of this study was to reveal the effects of Mg deficiency on Fe absorption,subcellular distribution,and chemical forms in citrus seedlings. Citrus sinensis‘Xuegan’sand cultured in greenhouse was used as experimental materials,which was treated with Mg sufficiency（2 mmol · L^-1 Mg²⁺）and Mg deficiency（0 mmol · L^-1 Mg²⁺）. After 11 months,the plant samples were collected to determine the biomass and Fe content,measure the Fe subcellular fractions and chemical forms,and calculate the Fe accumulation and distribution in different organs of citrus seedlings. Compared with Mg sufficiency treatment,the biomass of citrus plants was decreased by 40.32% under Mg deficiency treatment,which was decreased by 34.90%,39.61%,and 47.17% in leaf,stem and root,respectively. Mg deficiency treatment significantly increased Fe content in different organs,in which leaf,stem,and root increased by 25.32%,31.06%,and 53.94%,respectively. Also,Fe mainly accumulated in the root between treatments,which the proportion was more than 75%. Mg deficiency treatment significantly increased Fe content in different subcellular fractions,and it showed a general trend which was cell wall > organelle > soluble fraction between treatments,and the proportion of Fe content in the cell wall was more than 55% in branch leaves,upper leaves,lower leaves,and root tips. Moreover,Mg deficiency treatment not only significantly increased Fe content in various chemical forms in root tips,but also increased it in the phosphoric acid and oxalic acid forms and decreased it in the water-soluble form in leaf organs. However,the Fe mainly exists in the phosphoric acid and oxalic acid forms in different organs between treatments,which accounted for nearly 50%.

Key words: citrus, seedlings, Mg deficiency, biomass, Fe content, subcellular distribution, chemical forms

CLC Number:

S666

JIA Yamin, XU Hao, HU Wenlang, WANG Yuwen, YE Xin, CHEN Lisong, LI Yan, GUO Jiuxin. Magnesium Deficiency Altered in Iron Absorption,Subcellular Distribution,and Chemical Forms in Citrus Seedlings[J]. Acta Horticulturae Sinica, 2022, 49(5): 973-983.

Figures/Tables 7

Fig. 1 Diagram of sample collection in citrus seedlings

Fig. 2 Effects of Mg deficiency on biomass and proportion of various organs in citrus seedlings The different small letters in the same organ mean significant difference at 5% level. The same below.

Fig. 3 Effects of Mg deficiency on Fe content,accumulation,and proportion of various organs in citrus seedlings

Fig. 4 Effects of Mg deficiency on Fe content in subcellular of various organs in citrus seedlings

Fig. 5 Effects of Mg deficiency on Fe concentration in subcellular distribution of various organs in citrus seedlings

Fig. 6 Effects of Mg deficiency on Fe content in chemical forms of various organs in citrus seedlings

Fig. 7 Effects of Mg deficiency on Fe proportion in chemical forms of various organs in citrus seedlings

References 31

[1]	Briat J F, Dubos C, Gaymard F. 2015. Iron nutrition,biomass production,and plant product quality. Trends in Plant Science, 20:33-40. doi: 10.1016/j.tplants.2014.07.005 URL
[2]	Cai Y T, Zhang H, Qi Y P, Ye X, Huang Z R, Guo J X, Chen L S, Yang L T. 2019. Responses of reactive oxygen species and methylglyoxal metabolisms to magnesium-deficiency differ greatly among the roots,upper and lower leaves of Citrus sinensis. BMC Plant Biology, 19:76. doi: 10.1186/s12870-019-1683-4 pmid: 30770733
[3]	Cakmak I, Kirkby E A. 2008. Role of magnesium in carbon partitioning and alleviating photooxidative damage. Physiologia Plantarum, 133:692-704. doi: 10.1111/j.1399-3054.2007.01042.x URL
[4]	Cao Sheng, Ouyang Meng-yun, Zhou Wei-jun, Cui Hao-jie, Liu Pei, Tan Jie, Huang Lan, Liu Shuang. 2019. Multivariate analysis and modeling of fruit mineral nutrient,soil nutrient and pH in Satsuma mandarin citrus orchards in Hunan Province. Journal of Fruit Science, 36 (8):1029-1039. (in Chinese)
	曹胜, 欧阳梦云, 周卫军, 崔浩杰, 刘沛, 谭洁, 黄兰, 刘双. 2019. 湖南温州蜜柑果实矿质养分与土壤养分、pH的多元分析与模拟. 果树学报, 36 (8):1029-1039.
[5]	Chen Z C, Peng W T, Li J, Liao H. 2018. Functional dissection and transport mechanism of magnesium in plants. Seminars in Cell and Developmental Biology, 74:142-152. doi: 10.1016/j.semcdb.2017.08.005 URL
[6]	Farhat N, Elkhouni A, Zorrig W, Smaoui A, Abdelly C, Rabhi M. 2016. Effect of magnesium deficiency on photosynthesis and carbohydrate partitioning. Acta Physiologiae Plantarum, 38:145. doi: 10.1007/s11738-016-2165-z URL
[7]	Guo W, Nazim H, Liang Z, Yang D. 2016. Magnesium deficiency in plants:an urgent problem. The Crop Journal, 4:83-91. doi: 10.1016/j.cj.2015.11.003 URL
[8]	Hauer-Jákli M, Tränkner M. 2019. Critical leaf magnesium thresholds and the impact of magnesium on plant growth and photo-oxidative defense:a systematic review and meta-analysis from 70 years of research. Frontiers in Plant Science, 10:766. doi: 10.3389/fpls.2019.00766 pmid: 31275333
[9]	Hermans C, Conn S J, Chen J, Xiao Q, Verbruggen N. 2013. An update on magnesium homeostasis mechanisms in plants. Metallomics, 5:1170-1183. doi: 10.1039/c3mt20223b URL
[10]	Huang J H, Xu J, Ye X, Luo T Y, Ren L H, Fan G C, Qi Y P, Li Q, Ferrarezi R S, Chen L S. 2019. Magnesium deficiency affects secondary lignification of the vascular system in Citrus sinensis seedlings. Trees-Structure and Function, 33:171-182. doi: 10.1007/s00468-018-1766-0 URL
[11]	Huang Yu-yi, Liu Bin, Chen Gui-fen. 2006. Study on contents of soil available nutrients in citrus orchards in Guangxi. Southwest China Journal of Agricultural Sciences, 19 (5):863-866. (in Chinese)
	黄玉溢, 刘斌, 陈桂芬. 2006. 广西柑桔园土壤有效养分含量研究. 西南农业学报, 19 (5):863-866.
[12]	Huang Z R, Zhang H, Ye X, Lai N W, Yang L T, Guo J X, Chen L S. 2021. UHPLC-Q-TOF/MS-based metabolomics reveals altered metabolic profiles in magnesium deficiency leaves of Citrus sinensis. Scientia Horticulturae, 278:109870. doi: 10.1016/j.scienta.2020.109870 URL
[13]	Jin X L, Ma C L, Yang L T, Chen L S. 2016. Alterations of physiology and gene expression due to long-term magnesium-deficiency differ between leaves and roots of Citrus reticulata. Journal of Plant Physiology, 198:103-115. doi: 10.1016/j.jplph.2016.04.011 URL
[14]	Kobayashi N I, Ogura T, Takagi K, Sugita R, Suzuki H, Iwata R, Nakanishi T M, Tanoi K. 2018. Magnesium deficiency damages the youngest mature leaf in rice through tissue-specific iron toxicity. Plant and Soil, 428:137-152. doi: 10.1007/s11104-018-3658-x URL
[15]	Kobayashi T, Nozoye T, Nishizawa N K. 2019. Iron transport and its regulation in plants. Free Radical Biology and Medicine, 133:11-20. doi: S0891-5849(18)32257-3 pmid: 30385345
[16]	Li C P, Qi Y P, Zhang J, Yang L T, Wang D H, Ye X, Lai N W, Tan L L, Lin D, Chen L S. 2017. Magnesium-deficiency-induced alterations of gas exchange,major metabolites and key enzymes differ among roots,and lower and upper leaves of Citrus sinensis seedlings. Tree Physiology, 37:1564-1581. doi: 10.1093/treephys/tpx067 URL
[17]	Li Jun-cheng, Yu Hui, Yang Su-xin, Feng Xian-zhong. 2016. Research progress of molecular regulation of iron uptake in plants. Plant Physiology Journal, 52 (6):835-842. (in Chinese)
	李俊成, 于慧, 杨素欣, 冯献忠. 2016. 植物对铁元素吸收的分子调控机制研究进展. 植物生理学报, 52 (6):835-842.
[18]	Li Y, Han M Q, Lin F, Ten Y, Lin J, Zhu D, Guo P, Weng Y B, Chen L S. 2015. Soil chemical properties,‘Guanximiyou’pummelo leaf mineral nutrient status and fruit quality in the southern region of Fujian Province,China. Journal of Soil Science and Plant Nutrition, 15 (3):615-628.
[19]	Liu Hou-jun, Zhang Jun-ling, Han Xiao-ri, Zhang Fu-suo. 2009. Influences of iron plaque on element uptake by plants and its affecting factors. Soils, 41 (3):335-343. (in Chinese)
	刘侯俊, 张俊伶, 韩晓日, 张福锁. 2009. 根表铁膜对元素吸收的效应及其影响因素. 土壤, 41 (3):335-343.
[20]	Morton A R, Trolove S N, Kerekhoffs L J H. 2008. Magnesium deficiency in citrus grown in the Gisborne district of New Zealand. New Zealand Journal of Crop and Horticultural Science, 36:199-214. doi: 10.1080/01140670809510236 URL
[21]	Peng H Y, Qi Y P, Lee J, Yang L T, Guo P, Jiang H X, Chen L S. 2015. Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency. BMC Genomics, 16:253. doi: 10.1186/s12864-015-1462-z URL
[22]	Poorter H, Niklas K J, Reich P B, Oleksyn J, Poot P, Mommer L. 2012. Biomass allocation to leaves,stems and roots: meta-analyses of interspecific variation and environmental control. New Phytologist, 193:30-50. doi: 10.1111/j.1469-8137.2011.03952.x URL
[23]	Shen Yan, Xiao Jia-xin, Yang Hui, Zhang Shao-ling. 2011. Effects of magnesium stress on growth,distribution of several mineral elements and leaf ultrastructure of‘Harumi’tangor. Acta Horticulturae Sinica, 38 (5):849-858.
	申燕, 肖家欣, 杨慧, 张绍玲. 2011. 镁胁迫对‘春见’橘橙生长和矿质元素分布及叶片超微结构的影响. 园艺学报, 38 (5):849-858.
[24]	Su Y, Liu J, Lu Z, Wang X, Zhang Z, Shi G. 2014. Effects of iron deficiency on subcellular distribution and chemical forms of cadmium in peanut roots in relation to its translocation. Environmental and Experimental Botany, 97:40-48. doi: 10.1016/j.envexpbot.2013.10.001 URL
[25]	Vigani G, Solti Á, Thomine S, Philippar K. 2019. Essential and detrimental - an update on intracellular iron trafficking and homeostasis. Plant and Cell Physiology, 60 (7):1420-1439. doi: 10.1093/pcp/pcz091 URL
[26]	Wu S, Vymazal J, Brix H. 2019. Critical review:biogeochemical networking of iron in constructed wetlands for wastewater treatment. Environmental Science and Technology, 53:7930-7944. doi: 10.1021/acs.est.9b00958 URL
[27]	Yang L T, Zhou Y F, Wang Y Y, Wu Y M, Ye Xin, Guo J X, Chen L S. 2019. Magnesium deficiency induced global transcriptome change in Citrus sinensis leaves revealed by RNA-Seq. International Journal of Molecular Sciences, 20:3129. doi: 10.3390/ijms20133129 URL
[28]	Ye X, Chen X F, Deng C L, Deng C L, Yang L T, Lai N W, Guo J X, Chen L S. 2019. Magnesium-deficiency effects on pigments,photosynthesis and photosynthetic electron transport of leaves,and nutrients of leaf blades and veins in Citrus sinensis seedlings. Plants, 8:389. doi: 10.3390/plants8100389 URL
[29]	Zeng F, Ali S, Qiu B, Wu F, Zhang G. 2010. Effects of chromium stress on the subcellular distribution and chemical form of Ca,Mg,Fe,and Zn in two rice genotypes. Journal of Plant Nutrition and Soil Science, 173:135-148. doi: 10.1002/jpln.200900134 URL
[30]	Zhou Xin-bin, Shi Xiao-jun, Sun Peng-shou, Li Wei, Dai Heng-lin, Peng Liang-zhi, Chun Chang-pin. 2010. Status of soil fertility in citrus orchards of Chongqing Sanxia Reservoir rea. Journal of Plant Nutrition and Fertilizers, 16 (4):817-823. (in Chinese)
	周鑫斌, 石孝均, 孙彭寿, 李伟, 戴亨林, 彭良志, 淳长品. 2010. 三峡重庆库区柑橘园土壤养分丰缺状况研究. 植物营养与肥料学报, 16 (4):817-823.
[31]	Zhu Pan-pan, Li You-fang, Qiu Jie-ya, Wang Min, Zhang Chao-bo, Ling Li-li, Shi Yunting, Hu Jun-hua, Zhang Rong, Li Yong-qi, Zi Li-ling, Peng Liang-zhi. 2019. Correlation between soil and leaf nutrient element contents in citrus orchards of Yuxi City,Yunnan Province. Journal of Fruit Science, 36 (12):1658-1666. (in Chinese)
	朱攀攀, 李有芳, 邱洁雅, 王敏, 张超博, 凌丽俐, 施云庭, 胡军华, 张蓉, 李永奇, 资丽玲, 彭良志. 2019. 云南玉溪柑橘园土壤养分水平和叶片营养状况相关性分析. 果树学报, 36 (12):1658-1666.

Magnesium Deficiency Altered in Iron Absorption,Subcellular Distribution,and Chemical Forms in Citrus Seedlings

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