Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant（Camellia sinensis）

doi:10.16420/j.issn.0513-353x.2023-0128

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (1): 91-102.doi: 10.16420/j.issn.0513-353x.2023-0128

Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant（Camellia sinensis）

YE Yufan¹^,², WANG Yujie¹, FU Qianyuan¹^,², WANG Lu¹, HAO Xinyuan¹, DING Changqing¹, WANG Xinchao¹, CAO Hongli²^,³^,^**(), LI Nana¹^,^**

¹ Key Laboratory of Biology，Genetics and Breeding of Special Economic Animals and Plants，Ministry of Agriculture and Rural Affairs，National Center for Tea Improvement，Tea Research Institute，Chinese Academy of Agricultural Sciences，Hangzhou 310008，China
² Key Laboratory of Tea Science at Universities in Fujian，College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China
³ College of Food Science，Southwest University，Chongqing 400715，China

Received:2023-04-25 Revised:2023-08-28 Online:2024-01-25 Published:2024-01-16
Contact: **（E-mail：nanali@tricaas.com，lili9885@126.com）

Abstract

Abstract:

In this study，the complete open reading frame sequence of CsChlH gene encoded 1 382 amino acid residues was cloned in tea plant（NCBI accession number：ON256194）. Phylogenetic tree analysis showed that CsChlH was closely related to AcChlH of kiwifruit. Promoter cloning and sequence analysis showed that CsChlH gene may respond to multiple signals such as light，drought，heat shock，low temperature，ABA，ethylene，and may regulate the metabolism of chlorophylls，flavonoids，sugars，as well as the development of chloroplast，mesophyll，guard cell and other organs. CsChlH protein was localized in the chloroplast. Tissue-specific expression analysis found that CsChlH gene was highly expressed in the chlorophyll-rich source tissues but lowly expressed in the chlorophyll-deficient sink tissues. The expression of CsChlH gene was down-regulated in response to long-term low light conditions，cold and drought stresses，while up-regulated by short-term ABA，ethylene and long-term heat stress. The results revealed that CsChlH gene played a vital role in the regulation of chlorophyll metabolism and stress resistance of tea plant.

Key words: Camellia sinensis, CsChlH, promoter, gene expression, abscisic acid, GUS enzyme activity

YE Yufan, WANG Yujie, FU Qianyuan, WANG Lu, HAO Xinyuan, DING Changqing, WANG Xinchao, CAO Hongli, LI Nana. Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant（Camellia sinensis）[J]. Acta Horticulturae Sinica, 2024, 51(1): 91-102.

Figures/Tables 7

Table 1 Primers for cloning and expression analysis of CsChlH in tea plant

序列名称 Sequence name	上游引物序列（5′-3′） Forward primer	下游引物序列（5′-3′） Reverse primer	用途 Application
CsChlH ORF	CAGGAAACAGGTGAGTTTTCTGGAAG	TCCATCAAAGAGCAATAGAGAGATCAGG	扩增Amplification
CsChlH promoter	CACTACGAGGTTTACGAGATAAACAG	CTAAAGAAGCCATTGTCAATGCTCT	扩增Amplification
CsPTB ORF	ACCAAGCACACTCCACACTATCG	TGCCCCCTTATCATCATCCACAA	qRT-PCR
CsChlH ORF	TATGAGGGTGTGCGCGAAAT	GTCTCCCAATACCCACGTCC	qRT-PCR
CsChlH ORF	ACGAGAGTGTCGGAGCTCGGTACCATGGCTTCTTTAGTTTCTTCACCATTC	CTCGCCCTTGCTCACCATGTCGACACGATCAATCCCTTCAATCTTGTCTTC	亚细胞定位 Subcellular localization
CsChlH promoter	CAGGTCGACTCTAGAGGATCCCACTACGAGGTTTACGAGATAAACAG	CCTCAGATCTACCATGGTACCTGTCAATGCTCTCTCTGTTCTTCC	启动子连接GUS Promoter linked to GUS

Fig. 1 Open reading frame amplification（A），coding protein sequence（B）and gene structure（C）of CsChlH in tea plant The red underlined area is the protein conservative domain.

Fig. 2 PCR amplification（A）and sequence（B）of CsChlH promoter in tea plant

Fig. 3 Subcellular localization of CsChlH protein in tea plant A and E：GFP green fluorescence signal；B：RFP red fluorescence signal of nucleus；F：Chlorophyll autofluorescence；C and G：Bright field；D and H：Merged signal.

Fig. 4 Tissue-specific expression patterns of CsChlH in tea plant Different lowercase indicate significant differences（P < 0.05）. The same below.

Fig. 5 Expression levels of CsChlH under shading，phytomone，temperature，and drought treatments in tea plant * indicates significant differences between different treatments（P < 0.05）.

Fig. 6 GUS staining and enzyme activity detection of prCsChlH::GUS transgenic tobacco after drought treatment

References 41

[1]	Chen K, Li G J, Bressan R A, Song C P, Zhu J K, Zhao Y. 2020. Abscisic acid dynamics,signaling,and functions in plants. Journal of Integrative Plant Biology, 62 (1):25-54. doi: 10.1111/jipb.v62.1 URL
[2]	Du S Y, Zhang X F, Lu Z K, Xin Q, Wu Z, Jiang T, Lu Y, Wang X F, Zhang D P. 2012. Roles of the different components of magnesium chelatase in abscisic acid signal transduction. Plant Molecular Biology, 80 (4-5):519-537. doi: 10.1007/s11103-012-9965-3 URL
[3]	Hao X Y, Horvath D P, Chao W S, Yang Y J, Wang X C, Xiao B. 2014. Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant(Camellia sinensis (L.) O. Kuntze). International Journal of Molecular Sciences, 15 (12):22155-22172. doi: 10.3390/ijms151222155 URL
[4]	Higo K, Ugawa Y, Iwamoto M, Korenaga T. 1999. Plant cis-acting regulatory DNA elements(PLACE)database:1999. Nucleic Acids Research, 27 (1):297-300. doi: 10.1093/nar/27.1.297 pmid: 9847208
[5]	Hiriart J B, Lehto K, Tyystjärvi E, Junttila T, Aro E M. 2002. Suppression of a key gene involved in chlorophyll biosynthesis by means of virus-inducing gene silencing. Plant Molecular Biology, 50 (2):213-224. doi: 10.1023/A:1016000627231 URL
[6]	Huang C J, Hu G J, Li F F, Li Y Q, Wu J X, Zhou X P. 2013. NbPHAN,a MYB transcriptional factor,regulates leaf development and affects drought tolerance in Nicotiana benthamiana. Physiologia Plantarum, 149 (3):297-309. doi: 10.1111/ppl.2013.149.issue-3 URL
[7]	Huang J Y, Zhao X B, Bürger M, Chory J, Wang X C. 2023. The role of ethylene in plant temperature stress response. Trends in Plant Science, 28 (7):808-824. doi: 10.1016/j.tplants.2023.03.001 pmid: 37055243
[8]	Ibata H, Nagatani A, Mochizuki N. 2016. CHLH/GUN5 function in tetrapyrrole metabolism is correlated with plastid signaling but not ABA responses in guard cells. Frontiers in Plant Science, 7:1650. pmid: 27872634
[9]	Jespersen D, Zhang J, Huang B R. 2016. Chlorophyll loss associated with heat-induced senescence in bentgrass. Plant Science, 249:1-12. doi: S0168-9452(16)30069-3 pmid: 27297985
[10]	Jia H F, Chai Y M, Li C L, Qin L, Shen Y Y. 2011. Cloning and characterization of the H subunit of a magnesium chelatase gene(PpCHLH)in peach. Journal of Plant Growth Regulation, 30 (4):445-455. doi: 10.1007/s00344-011-9207-y URL
[11]	Jia Hai-feng, Chai Ye-mao, Li Chun-li, Dong Qing-hua, Qin Ling, Shen Yuan-yue. 2011. Expression changes of ABA receptor gene FaABAR/CHLH in strawberry and its affection factors. Acta Horticulturae Sinica, 38 (9):1650-1656. (in Chinese)
	贾海锋, 柴叶茂, 李春丽, 董清华, 秦岭, 沈元月. 2011. 草莓果实中脱落酸受体基因FaABAR/CHLH表达变化及其影响因素分析. 园艺学报, 38 (9):1650-1656.
[12]	Kindgren P, Dubreuil C, Strand Å. 2015. The recovery of plastid function is required for optimal response to low temperatures in Arabidopsis. PLoS ONE, 10 (9):e0138010. doi: 10.1371/journal.pone.0138010 URL
[13]	Li Jiajia, Yu Xudong, Cai Zeping, Wu Fanhua, Luo Jiajia, Zheng Liting, Chu Wenqing. 2019. An overview of chlorophyll biosynthesis in higher plants. Molecular Plant Breeding, 17 (18):6013-6019. (in Chinese)
	李佳佳, 于旭东, 蔡泽坪, 吴繁花, 罗佳佳, 郑李婷, 楚文清. 2019. 高等植物叶绿素生物合成研究进展. 分子植物育种, 17 (18):6013-6019.
[14]	Li Ke, Shen Mengxiao, Pan Weihao, Zhang Shixuan, Mao Xinye, Yin Yahong, Li Yongqiang, Zhu Youyin, Guo Weidong. 2023. Preliminary investigation of C₂H₂ family genes in blueberry flower bud dormancy release. Acta Horticulturae Sinica, 50 (4):737-753. (in Chinese)
	李可, 申朦晓, 潘炜浩, 章诗渲, 毛欣烨, 尹亚红, 李永强, 朱友银, 郭卫东. 2023. 蓝莓C₂H₂家族基因在花芽休眠解除中的作用初探. 园艺学报, 50 (4):737-753. doi: 10.16420/j.issn.0513-353x.2022-0044 URL
[15]	Li Na-na, Liu Ying, Zhang Hao-jie, Wang Lu, Hao Xin-yuan, Zhang Wei-fu, Wang Yu-chun, Xiong Fei, Yang Ya-jun, Wang Xin-chao. 2020. Promoter cloning and expression analysis of the hexokinase gene CsHXK2 in tea plant(Camellia sinensis). Acta Agronomica Sinica, 46 (10):1628-1638. (in Chinese) doi: 10.3724/SP.J.1006.2020.94166
	李娜娜, 刘莹, 张豪杰, 王璐, 郝心愿, 张伟富, 王玉春, 熊飞, 杨亚军, 王新超. 2020. 茶树己糖激酶基因CsHXK2的启动子克隆及表达特性分析. 作物学报, 46 (10):1628-1638. doi: 10.3724/SP.J.1006.2020.94166
[16]	Li Y S, Wang X Y, Zhang Q H, Shen Y B, Wang J, Qi S M, Zhao P, Muhammad T, Islam M M, Zhan X Q, Liang Y. 2022. A mutation in SlCHLH encoding a magnesium chelatase H subunit is involved in the formation of yellow stigma in tomato(Solanum lycopersicum L.). Plant Science, 325:111466. doi: 10.1016/j.plantsci.2022.111466 URL
[17]	Liang S, Lu K, Wu Z, Jiang S C, Yu Y T, Bi C, Xin Q, Wang X F, Zhang D P. 2015. A link between magnesium-chelatase H subunit and sucrose nonfermenting 1(SNF1)-related protein kinase SnRK2.6/OST1 in Arabidopsis guard cell signalling in response to abscisic acid. Journal of Experimental Botany, 66 (20):6355-6369. doi: 10.1093/jxb/erv341 pmid: 26175350
[18]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT Method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[19]	Ma C L, Chen L, Wang X C, Jin J Q, Ma J Q, Yao M Z, Wang Z L. 2012. Differential expression analysis of different albescent stages of‘Anji Baicha’(Camellia sinensis (L.) O. Kuntze)using cDNA microarray. Scientia Horticulturae, 148:246-254. doi: 10.1016/j.scienta.2012.09.033 URL
[20]	Mochizuki N, Brusslan J A, Larkin R, Nagatani A, Chory J. 2001. Arabidopsis genomes uncoupled 5(GUN5)mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction. Proceedings of the National Academy of Sciences of the United States of America, 98 (4):2053-2058.
[21]	Nott A, Jung H S, Koussevitzky S, Chory J. 2006. Plastid-to-nucleus retrograde signaling. Annual Review of Plant Biology, 57:739-759. pmid: 16669780
[22]	Ren J, Sun L, Wang C L, Zhao S L, Leng P. 2011. Expression analysis of the cDNA for magnesium chelatase H subunit(CHLH)during sweet cherry fruit ripening and under stress conditions. Plant Growth Regulation, 63 (3):301-307. doi: 10.1007/s10725-010-9530-5 URL
[23]	Shang Y, Yan L, Liu Z Q, Cao Z, Mei C, Xin Q, Wu F Q, Wang X F, Du S Y, Jiang T, Zhang X F, Zhao R, Sun H L, Liu R, Yu Y T, Zhang D P. 2010. The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition. Plant Cell, 22 (6):1909-1935. doi: 10.1105/tpc.110.073874 URL
[24]	Shen Y Y, Wang X F, Wu F Q, Du S Y, Cao Z, Shang Y, Wang X L, Peng C C, Yu X C, Zhu S Y, Fan R C, Xu Y H, Zhang D P. 2006. The Mg-chelatase H subunit is an abscisic acid receptor. Nature, 443 (7113):823-826. doi: 10.1038/nature05176
[25]	Tanaka A, Tanaka R. 2006. Chlorophyll metabolism. Current Opinion in Plant Biology, 9 (3):248-255. doi: 10.1016/j.pbi.2006.03.011 pmid: 16603411
[26]	Tanaka R, Tanaka A. 2007. Tetrapyrrole biosynthesis in higher plants. Annual Review of Plant Biology, 58:321-346. pmid: 17227226
[27]	Tian Y Y, Wang H Y, Zhang Z Q, Zhao X X, Wang Y, Zhang L X. 2022. An RNA-seq analysis reveals diferential transcriptional responses to diferent light qualities in leaf color of Camellia sinensis cv. Huangjinya. Journal of Plant Growth Regulation, 41 (2):612-627. doi: 10.1007/s00344-021-10325-2
[28]	Tsuzuki T, Takahashi K, Tomiyama M, Inoue S, Kinoshita T. 2013. Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana. Frontiers in Plant Science, 4:440. doi: 10.3389/fpls.2013.00440 pmid: 24198823
[29]	Wang Feng, Yan Jiarong, Chen Xueyu, Jiang Chenghao, Meng Sida, Liu Yufeng, Xu Tao, Qi Mingfang, Li Tianlai. 2019. Light regulation of chlorophyll biosynthesis in plants. Acta Horticulturae Sinica, 46 (5):975-994. (in Chinese) doi: 10.16420/j.issn.0513-353x.2018-0799 URL
	王峰, 闫家榕, 陈雪玉, 姜程浩, 孟思达, 刘玉凤, 许涛, 齐明芳, 李天来. 2019. 光调控植物叶绿素生物合成的研究进展. 园艺学报, 46 (5):975-994. doi: 10.16420/j.issn.0513-353x.2018-0799 URL
[30]	Wang X C, Feng H, Chang Y X, Ma C L, Wang L Y, Hao X Y, Li A L, Cheng H, Wang L, Cui P, Jin J Q, Wang X B, Wei K, Ai C, Zhao S, Wu Z C, Li Y Y, Liu B Y, Wang G D, Chen L, Ruan J, Yang Y J. 2020. Population sequencing enhances understanding of tea plant evolution. Nature Communications, 11 (1):4447. doi: 10.1038/s41467-020-18228-8 pmid: 32895382
[31]	Wang Xiaochen, Nie Ziye, Liu Xianju, Duan Wei, Fan Peige, Liang Zhenchang. 2023. Effects of abscisic acid on monoterpene synthesis in‘Jingxiangyu’grape berries. Acta Horticulturae Sinica, 50 (2):237-249. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-1117 URL
	王晓晨, 聂子页, 刘先菊, 段伟, 范培格, 梁振昌. 2023. 脱落酸对‘京香玉’葡萄果实单萜物质合成的影响. 园艺学报, 50 (2):237-249. doi: 10.16420/j.issn.0513-353x.2021-1117 URL
[32]	Wu F Q, Xin Q, Cao Z, Liu Z Q, Du S Y, Mei C, Zhao C X, Wang X F, Shang Y, Jiang T, Zhang X F, Yan L, Zhao R, Cui Z N, Liu R, Sun H L, Yang X L, Su Z, Zhang D P. 2009. The magnesium-chelatase H subunit binds abscisic acid and functions in abscisic acid signaling:new evidence in Arabidopsis. Plant Physiology, 150 (4):1940-1954. doi: 10.1104/pp.109.140731 URL
[33]	Wu Y, Jin X, Liao W B, Hu L L, Dawuda M M, Zhao X J, Tang Z Q, Gong T Y, Yu J H. 2018. 5-Aminolevulinic acid(ALA)alleviated salinity stress in cucumber seedlings by enhancing chlorophyll synthesis pathway. Frontiers in Plant Science, 9:635. doi: 10.3389/fpls.2018.00635 URL
[34]	Xie S L, Nie L B, Zheng Y S, Wang J, Zhao M R, Zhu S D, Hou J F, Chen G H, Wang C G, Yuan L Y. 2019. Comparative proteomic analysis reveals that chlorophyll metabolism contributes to leaf color changes in wucai(Brassica campestris L.)responding to cold acclimation. Journal of Proteome Research, 18 (6):2478-2492. doi: 10.1021/acs.jproteome.9b00016 URL
[35]	Yin X R, Xie X L, Xia X J, Yu J Q, Ferguson I B, Giovannoni J J, Chen K S. 2016. Involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening. Plant Journal, 86 (5):403-412. doi: 10.1111/tpj.2016.86.issue-5 URL
[36]	Yuan L Y, Zhang L T, Wu Y, Zheng Y S, Nie L B, Zhang S N, Lan T, Zhao Y, Zhu S D, Hou J F, Chen G H, Tang X Y, Wang C G. 2021. Comparative transcriptome analysis reveals that chlorophyll metabolism contributes to leaf color changes in wucai(Brassica campestris L.)in response to cold. BMC Plant Biology, 21 (1):438. doi: 10.1186/s12870-021-03218-9
[37]	Zhang X F, Jiang T, Yu Y T, Wu Z, Jiang S C, Lu K, Feng X J, Liang S, Lu Y F, Wang X F, Zhang D P. 2014. Arabidopsis co-chaperonin CPN20 antagonizes Mg-chelatase H subunit to derepress ABA-responsive WRKY 40 transcription repressor. Science China-Life Sciences, 57 (1):11-21. doi: 10.1007/s11427-013-4587-9 URL
[38]	Zhang X T, Chen S, Shi L Q, Gong D P, Zhang S C, Zhao Q, Zhan D L, Vasseur L, Wang Y B, Yu J X, Liao Z Y, Xu X D, Qi R, Wang W L, Ma Y R, Wang P J, Ye N X, Ma D N, Shi Y, Wang H F, Ma X K, Kong X R, Lin J, Wei L F, Ma Y Y, Li R Y, Hu G P, He H F, Zhang L, Ming R, Wang G, Tang H B, You M S. 2021. Haplotype-resolved genome assembly provides insights into evolutionary history of the tea plant Camellia sinensis. Nature Genetics, 53 (8):1250-1259. doi: 10.1038/s41588-021-00895-y
[39]	Zhang Yin, Hu Luyan, Wang Shuming, Jing Danlong, Guo Qigao, Liang Guolu. 2023. Research advances in ABA-mediated fruit ripening. Acta Horticulturae Sinica, 50 (9):1889-1898. (in Chinese) doi: 10.16420/j.issn.0513-353x.2023-0288 URL
	张印, 胡路艳, 王淑明, 景丹龙, 郭启高, 梁国鲁. 2023. ABA调控果实成熟研究进展. 园艺学报, 50 (9):1889-1898. doi: 10.16420/j.issn.0513-353x.2023-0288 URL
[40]	Zhao C J, Liu L J, Safdar L B, Xie M L, Cheng X H, Liu Y Y, Xiang Y, Tong C B, Tu J X, Huang J Y, Liu S Y. 2020. Characterization and fine mapping of a yellow-virescent gene regulating chlorophyll biosynthesis and early stage chloroplast development in Brassica napus. G3-Genes Genomes Genetics, 10 (9):3201-3211. doi: 10.1534/g3.120.401460 URL
[41]	Zoschke R, Chotewutmontri P, Barkan A. 2017. Translation and co-translational membrane engagement of plastid-encoded chlorophyll-binding proteins are not influenced by chlorophyll availability in maize. Frontiers in Plant Science, 8:385. doi: 10.3389/fpls.2017.00385 pmid: 28400776

Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant（Camellia sinensis）

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