Cloning and Cd-resistant Analysis of PsWRKY40 in Potentilla sericea

doi:10.16420/j.issn.0513-353x.2022-0213

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (6): 1269-1283.doi: 10.16420/j.issn.0513-353x.2022-0213

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Cloning and Cd-resistant Analysis of PsWRKY40 in Potentilla sericea

GAO Pengfei, GAO Bing, FENG Zhenghong, WU Jianhui()

College of Landscape Architecture，Northeast Forestry University，Harbin 150040，China

Received:2022-12-27 Revised:2023-03-13 Online:2023-06-25 Published:2023-06-27
Contact: * （E-mail：wujianhui660915@126.com）

Abstract

Abstract:

The full-length cDNA sequence of PsWRKY40 was cloned from Potentilla sericea. The open reading frame of this gene was 978 bp in length，which encoded 325 amino acids，and belonged to the GroupⅡsubfamily of the WRKY family. The expression level of PsWRKY40 significantly increased by NaCl，CdCl₂ and mannitol treatments. PsWRKY40 was transformed into Arabidopsis thaliana heterologously，and transgenic plant was obtained. Under the treatment of Cd²⁺，it was found that the resistance of the transgenic lines was significantly higher than that of the wild type，and transgenic lines had a significantly higher expression level of AtSOD，AtPOD and AtCAT. The results showed that PsWRKY40 can be induced by Cd²⁺，so it may participate in the regulation of Cd²⁺ resistance in Potentilla sericea.

Key words: Potentilla sericea, WRKY, cadmium stress, gene cloning, functional analysis

CLC Number:

S682

GAO Pengfei, GAO Bing, FENG Zhenghong, WU Jianhui. Cloning and Cd-resistant Analysis of PsWRKY40 in Potentilla sericea[J]. Acta Horticulturae Sinica, 2023, 50(6): 1269-1283.

Figures/Tables 12

Table 1 List of primer sequences

引物名称 Primer name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
PsWRKY40	TAGGTTCGTTTCGGGTTCAT	CTGGAGAGTTAGGCTACAAGTCAC
PsWRKY40-qPCR	GCTGCAAGTGCTTTGGTGAA	CCCTGCTCAACCTCTCTTGG
β-Actin	GCGACAATGGAACTGGAATGG	GACAATTTCCCGTTCAGCAGTG
pBI121-PsWRKY40	GCTCTAGAATGGACTACTCA	TTGCCAAATGTTTGAACGATC
Actin8	TCAGCACTTTCCAGCAGATG	CTGTGGACAATGCCTGGAC
AtSOD	AGGAAACATCACTGTTGGAGAT	GAGTTTGGTCCAGTAAGAGGAA
AtPOD	CGTGCCCTTCATATTGTTGG	GACGCCATCAACAACGAGTC
AtCAT	AGGATCAAACTTTGAGGGGTAG	CTTGTGGTTCCTGGAATCTACT

Fig. 1 PCR results of full-length PsWRKY40

Fig. 2 Phylogenetic tree analysis of PsWRKY40 The number represents credibility.

Fig. 3 Expression patterns of PsWRKY40 under different abiotic stresses Different lowercase letters indicate that there is a significant difference (P < 0.05).

Fig. 4 Screening of transgenic Arabidopsis seeds T0-T3 for kanamycin resistance

Fig. 5 PCR（A）and qPCR（B）identification of PsWRKY40 transgenic Arabidopsis thaliana CK is agrobacterium positive control, WT is wild-type Arabidopsis thaliana, ddH2O is negative control，OE-1-OE-6 are transgenic lines. Different lowercase letters indicate that there is a significant difference（P < 0.05）.

Fig. 6 Seed germination（A）and plant growth status（B）of PsWRY40 transgenic Arabidopsis thaliana（OE）and its wild type（WT）under CdCl2 treatments

Table 2 Seed germination rate of PsWRKY40 transgenic Arabidopsis thaliana (OE) and its wild tye (WT) treated with different concentrations of CdCl2 %

株系 Plant line	CdCl₂/（μmol · L^-1）
株系 Plant line	0	50	100	150
WT	97.33 ± 2.67 a	66.67 ± 3.53 b	21.63 ± 1.21 d	3.92 ± 2.15 f
OE-1	98.61 ± 1.39 a	96.14 ± 2.14 a	47.79 ± 3.26 c	15.17 ± 2.13 de
OE-2	100.00 ± 0.00 a	97.53 ± 2.47 a	49.95 ± 2.86 c	16.69 ± 2.61 de
OE-6	97.38 ± 1.31 a	96.00 ± 2.31 a	45.01 ± 4.03 c	11.71 ± 2.56 e

Fig. 7 Oxidative stress condition of PsWRY40 transgenic Arabidopsis thaliana（OE）and its wild type（WT）under CdCl2 treatment Different lowercase letters indicate that there is a significant difference（P < 0.05）. The same below.

Fig. 8 Antioxidative enzyme activity and chlorophyll content of PsWRY40 transgenic Arabidopsis thaliana（OE）and its wild type（WT）under CdCl2 treatment

Fig. 9 Antioxidant-related gene expression of PsWRY40 transgenic Arabidopsis thaliana（OE）and its wild type（WT）under CdCl2 treatment

Fig. 10 Correlation coefficient between PsWRKY40 expression level and related gene expression, enzyme activity and chlorophyll content in transgenic Arabidopsis under CdCl2 treatment “*”represents significant at P < 0.05，“**”represent significant at P < 0.01.

References 50

[1]	Cai Z D, Xian P Q, Wang H, Lin R B, Lian T X, Cheng Y B, Ma Q B, Nian H. 2020. Transcription factor GmWRKY 142 confers cadmium resistance by up-regulating the cadmium tolerance 1-like genes. Frontiers in Plant Science, 11:724. doi: 10.3389/fpls.2020.00724 URL
[2]	Chen Bin, Wang Ya-nan, Ma Dan-wei, Hu Zhong-liang, He Ya-qiang, Zhou Jian. 2015. The antioxidant enzyme activities and their gene expression in maize radicle under the allelochemical stress from Chenopodium ambrosioides L. Ecology and Environmental Sciences, 24 (10):1640-1646. (in Chinese)
	陈斌, 王亚男, 马丹炜, 胡忠良, 何亚强, 周健. 2015. 土荆芥化感胁迫对玉米幼根抗氧化酶活性和基因表达的影响. 生态环境学报, 24 (10):1640-1646. doi: 10.16258/j.cnki.1674-5906.2015.10.008
[3]	Chen H, Lai Z B, Shi J W, Xiao Y, Chen Z X, Xu X P. 2010. Roles of Arabidopsis WRKY18,WRKY40 and WRKY 60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biology, 10:281. doi: 10.1186/1471-2229-10-281 pmid: 21167067
[4]	Chen Xia, Luo Shi-qiao, Duan Cui-fang, Zeng Ri-zhong. 2008. Research advance on the transcription factors in higher plants. Anhui Agricultural Science Bulletin,(9):48-52,65. (in Chinese)
	陈霞, 罗世巧, 段翠芳, 曾日中. 2008. 高等植物转录因子研究进展. 安徽农学通报,(9):48-52,65.
[5]	Fan Wei-Fang. 2021. Transcriptome analysis of Potentilla sericea and preliminary study on PsMYB2 function under cadmium stress[M. D. Dissertation]. Harbin: Northeast Forestry University. (in Chinese)
	范卫芳. 2021. 镉胁迫下绢毛委陵菜转录组分析及PsMYB2转录因子功能初步研究[硕士论文]. 哈尔滨: 东北林业大学.
[6]	Farmer E E, Mueller M J. 2013. ROS-mediated lipid peroxidation and RES-activated signaling. Annu Rev Plant Biol, 64:429-450. doi: 10.1146/annurev-arplant-050312-120132 pmid: 23451784
[7]	Han Y Y, Fan T T, Zhu X Y, Wu X, Ouyang J, Jiang L, Cao S Q. 2019. WRKY12 represses GSH1 expression to negatively regulate cadmium tolerance in Arabidopsis. Plant Mol Biol, 99 (1-2):149-159. doi: 10.1007/s11103-018-0809-7
[8]	Hong C Y, Cheng D, Zhang G Q, Zhu D D, Chen Y H, Tan M P. 2017. The role of ZmWRKY4 in regulating maize antioxidant defense under cadmium stress. Biochemical and Biophysical Research Communications, 482 (4):1504-1510. doi: 10.1016/j.bbrc.2016.12.064 URL
[9]	Jia Z Z, Li M Z, Wang H C, Zhu B, Gu L, Du X Y, Ren M J. 2021. TaWRKY70 positively regulates TaCAT5 enhanced Cd tolerance in transgenic Arabidopsis. Environmental and Experimental Botany, 190:104591. doi: 10.1016/j.envexpbot.2021.104591 URL
[10]	Li J, Besseau S, Toronen P, Sipari N, Kollist H, Holm L, Palva E T. 2013. Defense-related transcription factors WRKY70 and WRKY 54 modulate osmotic stress tolerance by regulating stomatal aperture in Arabidopsis. New Phytologist, 200 (2):457-472. doi: 10.1111/nph.2013.200.issue-2 URL
[11]	Li S J, Fu Q T, Huang W D, Yu D Q. 2009. Functional analysis of an Arabidopsis transcription factor WRKY 25 in heat stress. Plant Cell Rep, 28 (4):683-693. doi: 10.1007/s00299-008-0666-y URL
[12]	Liu Y J, Yu X F, Feng Y M, Zhang C, Wang C, Zeng J, Huang Z, Kang H Y, Fan X, Sha L N, Zhang H Q, Zhou Y H, Gao S P, Chen Q B. 2017. Physiological and transcriptome response to cadmium in cosmos(Cosmos bipinnatus Cav.)seedlings. Scientific Reports, 7 (1):14691. doi: 10.1038/s41598-017-14407-8
[13]	Liu Z Q, Lu Y, Zhen W, Mei C, Kai L, Yu Y T, Shan L, Zhang X F, Wang X F, Zhang D P. 2012. Cooperation of three WRKY-domain transcription factors WRKY18, WRKY40, and WRKY60 in repressing two ABA-responsive genes ABI4 and ABI5 in Arabidopsis. Journal of Experimental Botany, 63 (18):18.
[14]	Lu Q H, Xu Z D, Xu X H, Liu L, Liang L C, Chen Z, Dong X, Li C, Wang Y J, Qiu G L. 2019. Cadmium contamination in a soil-rice system and the associated health risk:an addressing concern caused by barium mining. Ecotoxicology and Environmental Safety, 183:109590. doi: 10.1016/j.ecoenv.2019.109590 URL
[15]	Ma Y L, Wang H F, Wang P, Yu C G, Luo S Q, Zhang Y F, Xie Y F. 2018. Effects of cadmium stress on the antioxidant system and chlorophyll fluorescence characteristics of two Taxodium clones. Plant Cell Reports, 37 (11):1547-1555. doi: 10.1007/s00299-018-2327-0
[16]	Meng Xing-nan. 2021. Functional analysis of ERF5gene responding to cadmium stress in Arabidopsis[M. D. Dissertation]. Hefei: Hefei University of Technology. (in Chinses)
	孟杏楠. 2021. ERF5基因在拟南芥响应镉胁迫中的功能分析[硕士论文]. 合肥: 合肥工业大学.
[17]	Meng Yun. 2019. Mechanism by which WRKY47 gene regulating the cadmium stress response in Arabidopsis thaliana [M. D. Dissertation]. Hefei: Hefei University of Technology.
	孟云. 2019. 拟南芥WRKY47基因在镉胁迫应答中的作用机理研究[硕士论文]. 合肥: 合肥工业大学.
[18]	Pandey S P, Roccaro M, Schön M, Logemann E, Somssich I E. 2010. Transcriptional reprogramming regulated by WRKY18 and WRKY40 facilitates powdery mildew infection of Arabidopsis. The Plant Journal, 64 (6):912-923. doi: 10.1111/j.1365-313X.2010.04387.x pmid: 21143673
[19]	Peng Ling, Jia Fen, Tian Xiao-ping, Yang Jing, Qu Chan-juan, Zhao Xiao-hu. 2015. Alleviation of cadmium stress on root tip of rape seedlings by selenium. Acta Scientiae Circumstantiae, 35 (8):2597-2604. (in Chinese)
	彭玲, 贾芬, 田小平, 杨晶, 屈婵娟, 赵小虎. 2015. 硒对油菜根尖镉胁迫的缓解作用. 环境科学学报, 35 (8):2597-2604.
[20]	Peng Xi-xu, Bai Ning-ning, Wang Hai-hua. 2018. Isolation and expression profiles of cadmium stress-responsive rice WRKY15 transcription factor gene. Chinese Rice Science, 32 (2):103-110. (in Chinese)
	彭喜旭, 白宁宁, 王海华. 2018. 响应镉胁迫的水稻WRKY15转录因子基因的分离与表达特征. 中国水稻科学, 32 (2):103-110. doi: 10.16819/j.1001-7216.2018.7056
[21]	Ren Yuan-qing. 2018. The functions of wheat expansin gene TaEXPA2in Cd stress tolerance[M. D. Dissertation]. Taian: Shandong Agricultural University. (in Chinses)
	任元卿. 2018. 小麦扩展蛋白基因TaEXPA2在镉胁迫耐性中的功能分析[硕士论文]. 泰安: 山东农业大学.
[22]	Robatzek S, Somssich I E. 2002. Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes & Development, 16 (9):1139-1149. doi: 10.1101/gad.222702 URL
[23]	Rushton P J, Somssich I E, Ringler P, Shen Q J. 2010. WRKY transcription factors. Trends Plant Science, 15 (5):247-258. doi: 10.1016/j.tplants.2010.02.006 URL
[24]	Shang Jing. 2021. Preliminary identification of salt tolerance function of SmWRKY40 transcription factor from eggplant[M. D. Dissertation]. Shanghai: Shanghai Ocean University. (in Chinese)
	尚静. 2021. 茄子SmWRKY40转录因子耐盐功能的初步鉴定[硕士论文]. 上海: 上海海洋大学.
[25]	Sheng Y B, Yan X X, Huang Y Y, Han Y Y, Zhang C, Ren Y B, Fan T T, Xiao F M, Liu Y S, Cao S Q. 2019. The WRKY transcription factor,WRKY13,activates PDR8 expression to positively regulate cadmium tolerance in Arabidopsis. Plant Cell Environment, 42 (3):891-903. doi: 10.1111/pce.v42.3 URL
[26]	Shi T R, Zhang Y Y, Gong Y W, Ma J, Wei H Y, Wu X, Zhao L, Hou H. 2019. Status of cadmium accumulation in agricultural soils across China (1975—2016):From temporal and spatial variations to risk assessment. Chemosphere, 230:136-143. doi: 10.1016/j.chemosphere.2019.04.208 URL
[27]	Siddiqui M H, Al-Whaibi M H, Sakran A M, Basalah M O, Ali H M. 2012. Effect of calcium and potassium on antioxidant system of Vicia faba L. under cadmium stress. International Journal of Molecular Sciences, 13 (6):6604-6619. doi: 10.3390/ijms13066604 pmid: 22837652
[28]	Sofo A, Bochicchio R, Amato M, Rendina N, Vitti A, Nuzzaci M, Altamura M M, Falasca G, Rovere F D, Scopa A. 2017. Plant architecture,auxin homeostasis and phenol content in Arabidopsis thaliana grown in cadmium- and zinc-enriched media. Journal of Plant Physiology, 216:174-180. doi: 10.1016/j.jplph.2017.06.008 URL
[29]	Tong Chen-chen, Zhang Cheng, Fan Ting-ting, Cao Shu-qing. 2020. Cloning and expression analysis of AtWRKY33 gene promoter from Arabidopsis thaliana. Journal of Anhui Agricultural University, 47 (5):784-788. (in Chinese)
	童晨晨, 张乘, 樊婷婷, 曹树青. 2020. 拟南芥AtWRKY33基因启动子的克隆及表达分析. 安徽农业大学学报, 47 (5):784-788.
[30]	Wang Ru-yue, Wen Wu-wu, Zhao En-hua, Zhou Peng, An Yuan. 2021. Cloning and salt-tolerance analysis of MsWRKY11 in alfalfa. Acta Prataculturae Sinica, 30 (11):157-169. (in Chinese) doi: 10.11686/cyxb2020426
	王如月, 文武武, 赵恩华, 周鹏, 安渊. 2021. 紫花苜蓿MsWRKY11基因的克隆及其耐盐功能分析. 草业学报, 30 (11):157-169. doi: 10.11686/cyxb2020426
[31]	Wang Xue-kui. 2006. Principles and techniques of plant physiological and biochemical experiments. Beijing: Higher Education Press. (in Chinese)
	王学奎. 2006. 植物生理生化实验原理和技术. 北京: 高等教育出版社.
[32]	Wang Y Y, Feng L, Zhu Y X, Li Y, Yan H W, Xiang Y. 2015. Comparative genomic analysis of the WRKY III gene family in Populus,grape,Arabidopsis and rice. Biology Direct, 10:48. doi: 10.1186/s13062-015-0076-3 URL
[33]	Wei J, Zheng X G, Liu J T, Zhang G W, Zhang Y X, Wang C L, Liu Y C. 2021. The levels,sources,and spatial distribution of heavy metals in soils from the drinking water sources of Beijing,China. Sustainability, 13 (7):3719. doi: 10.3390/su13073719 URL
[34]	Wu Xia, Zhang Yi-nan, Zhao Nan, Zhang Ying, Zhao Rui, Li Jin-ke, Zhou Xiao-yang, Chen Shao-liang. 2020. Overexpression of PeAnn1 from Populus euphratica negatively regulates drought resistance in transgenic Arabidopsis thaliana. Journal of Beijing Forestry University, 42 (6):14-25. (in Chinese)
	武霞, 张一南, 赵楠, 张莹, 赵瑞, 李金克, 周晓阳, 陈少良. 2020. 过表达胡杨PeAnn1负调控拟南芥的抗旱性. 北京林业大学学报, 42 (6):14-25.
[35]	Xie Z, Zhang Z L, Zou X, Huang J, Ruas P, Thompson D, Shen Q J. 2005. Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells. Plant Physiology, 137 (1):176-189. doi: 10.1104/pp.104.054312 pmid: 15618416
[36]	Xiong L, Schumaker K S, Zhu J K. 2002. Cell signaling during cold,drought,and salt stress. Plant Cell, 14 (Suppl):S165-183. doi: 10.1105/tpc.000596 URL
[37]	Xu J, Duan X, Yang J, Beeching J R, Zhang P. 2013. Coupled expression of Cu/Zn-superoxide dismutase and catalase in cassava improves tolerance against cold and drought stresses. Plant Signaling & Behavior, 8 (6):e24525.
[38]	Xu J, Yang J, Duan X G, Jiang Y M, Zhang P. 2014. Increased expression of native cytosolic Cu/Zn superoxide dismutase and ascorbate peroxidase improves tolerance to oxidative and chilling stresses in cassava (Manihot esculenta Crantz). BMC Plant Biology, 14:208. doi: 10.1186/s12870-014-0208-4 pmid: 25091029
[39]	Yang C L, Zhou Y, Fan J, Fu Y H, Shen L B, Yao Y, Li R M, Fu S P, Duan R J, Hu X W, Guo J C. 2015. SpBADH of the halophyte Sesuvium portulacastrum strongly confers drought tolerance through ROS scavenging in transgenic Arabidopsis. Plant Physiology and Biochemistry, 96:377-387. doi: 10.1016/j.plaphy.2015.08.010 URL
[40]	Yang Gui-yan. 2014. Salt and cadmium tolerance mechanism analysis of ThVHAc1 from Tamarix hispida[Ph. D. Dissertation]. Harbin: Northeast Forestry University. (in Chinses)
	杨桂燕. 2014. 柽柳ThVHAc1基因耐盐及抗镉机制研究[博士论文]. 哈尔滨: 东北林业大学.
[41]	Yang G Y, Wang C, Wang Y C, Guo Y C, Zhao Y L, Yang C P, Gao C Q. 2016. Overexpression of ThVHAc1 and its potential upstream regulator,ThWRKY7,improved plant tolerance of cadmium stress. Scientific Reports, 6:18752. doi: 10.1038/srep18752
[42]	Yu Fang-ming, Tang Ye-tao, Qiu Rong-liang, Zhou Xiao-yong, Ying Rong-rong, Hu Peng-jie, Zhang Tao. 2010. Antioxidative responses to cadmium stress in the hyperaccumulator Arabis paniculata Franch. Acta Scientiae Circumstantiae, 30 (2):409-414. (in Chinese)
	于方明, 汤叶涛, 仇荣亮, 周小勇, 应蓉蓉, 胡鹏杰, 张涛. 2010. Cd胁迫下超富集植物圆锥南芥抗氧化机理. 环境科学学报, 30 (2):409-414.
[43]	Zentella R, Zhang Z L, Park M, Thomas S G, Endo A, Murase K, Fleet C M, Jikumaru Y, Nambara E, Kamiya Y, Sun T P. 2007. Global analysis of della direct targets in early gibberellin signaling in Arabidopsis. Plant Cell, 19 (10):3037-3057. doi: 10.1105/tpc.107.054999 pmid: 17933900
[44]	Zhang Hui-long, Wu Xia, Yao Jun, Zhao Nan, Zhao Rui, Li Jin-ke, Shen Xin, Chen Shao-liang. 2019. Overexpression mechanism of PeREM1.3 from Populus euphratica enhancing salt tolerance in transgenic tobacco. Journal of Beijing Forestry University, 41 (1):1-9. (in Chinese)
	张会龙, 武霞, 尧俊, 赵楠, 赵瑞, 李金克, 沈昕, 陈少良. 2019. 胡杨 PeREM1.3过表达提高烟草耐盐性的机制. 北京林业大学学报, 41 (1):1-9.
[45]	Zhang W W, Song J F, Yue S Q, Duan K X, Yang H Q. 2019. MhMAPK4 from Malus hupehensis Rehd. decreases cell death in tobacco roots by controlling Cd²⁺uptake. Ecotoxicology and Environmental Safety, 168:230-240. doi: 10.1016/j.ecoenv.2018.09.126 URL
[46]	Zhang Xu, Ling Hui, Liu Feng, Huang Ning, Wang Ling, Mao Hua-ying, Li Cong-na, Tang Han-chen, Su Wei-hua, Su Ya-chun, Que You-xiong. 2018. Cloning and expression analysis of a Ⅱd sub-group WRKY transcription factor gene from sugarcane. Chinese Agricultural Science, 51 (23):4409-4423. (in Chinese)
	张旭, 凌辉, 刘峰, 黄宁, 王玲, 毛花英, 李聪娜, 汤翰臣, 苏炜华, 苏亚春, 阙友雄. 2018. 一个甘蔗Ⅱd类WRKY转录因子基因的克隆和表达分析. 中国农业科学, 51 (23):4409-4423. doi: 10.3864/j.issn.0578-1752.2018.23.002
[47]	Zhang X, Yan Y, Wadood S A, Sun Q, Guo B. 2020. Source apportionment of cadmium pollution in agricultural soil based on cadmium isotope ratio analysis. Applied Geochemistry, 123 (1):104776. doi: 10.1016/j.apgeochem.2020.104776 URL
[48]	Zhao X, Yang X W, Pei S Q, He G, Wang X Y, Tang Q, Jia C L, Lu Y, Hu R B, Zhou G K. 2016. The Miscanthus NAC transcription factor MlNAC 9 enhances abiotic stress tolerance in transgenic Arabidopsis. Gene, 586 (1):158-169. doi: 10.1016/j.gene.2016.04.028 pmid: 27085481
[49]	Zhou J T, Wan H X, He J L, Lyu D G, Li H F. 2017. Integration of cadmium accumulation,subcellular distribution,and physiological responses to understand cadmium tolerance in apple rootstocks. Frontiers Plant Science, 8:966. doi: 10.3389/fpls.2017.00966 URL
[50]	Zhu H H, Ai H L, Cao L W, Sui R, Ye H P, Du D Y, Sun J, Yao J, Chen K, Chen L. 2018. Transcriptome analysis providing novel insights for Cd-resistant tall fescue responses to Cd stress. Ecotoxicology and Environmental Safety, 160:349-356. doi: S0147-6513(18)30455-X pmid: 29860131

Cloning and Cd-resistant Analysis of PsWRKY40 in Potentilla sericea

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