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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1706-1716.doi: 10.16420/j.issn.0513-353x.2020-0961

• Research Papers • Previous Articles     Next Articles

Garlic Circadian Clock Genes AsRVE1 and AsRVE2 and Their Expression Analysis Under Osmotic Stress

BIAN Shicun1, LU Yani1, XU Wujun1, CHEN Boqing2, WANG Guanglong2,*(), XIONG Aisheng3,*()   

  1. 1Faculty of Applied Technology,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China
    2School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China
    3College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-05-13 Revised:2021-07-31 Online:2021-09-25 Published:2021-09-30
  • Contact: WANG Guanglong,XIONG Aisheng E-mail:guanglongwang@hyit.edu.cn;xiongaisheng@njau.edu.cn

Abstract:

In order to understand the sequence characteristics of circadian clock-related genes REVEILLEsRVEs)in garlic and their roles in osmotic stress response,AsRVE1 and AsRVE2 genes were isolated from garlic,and their expression patterns under salt stress and simulated drought stress were analyzed by qRT-PCR. Sequence analysis showed that the open reading frames of AsRVE1 and AsRVE2 genes were 1 050 and 975 bp in length,encoding 349 and 324 amino acids,respectively. In terms of evolutionary relationship,AsRVE1 and AsRVE2 were relatively close to ZmRVE2 in corn and AcRVE2 in pineapple. The RVE amino acid sequences of different plants had low homology,but showed high consistency in the conserved domain at the N-terminus. Both AsRVE1 and AsRVE2 genes can respond to changes in circadian rhythms,and their expression can be detected in different tissues;the expression of AsRVE1 in different tissues was not obviously different,the expression of AsRVE2 in the roots was relatively high. Both drought stress and salt stress induced the altered expression of AsRVE1 and AsRVE2 genes in different tissues. These results indicated that AsRVE1 and AsRVE2 genes may be involved in drought- and salt-stress response in garlic. The AsRVE1 and AsRVE2 genes cloned in this study laid a foundation for further identification of the biological functions and regulatory mechanisms of RVE.

Key words: garlic, circadian rhythm, RVE transcription factor, osmotic stress, gene cloning, expression analysis

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