Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1706-1716.doi: 10.16420/j.issn.0513-353x.2020-0961
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BIAN Shicun1, LU Yani1, XU Wujun1, CHEN Boqing2, WANG Guanglong2,*(), XIONG Aisheng3,*()
Received:
2021-05-13
Revised:
2021-07-31
Online:
2021-09-25
Published:
2021-09-30
Contact:
WANG Guanglong,XIONG Aisheng
E-mail:guanglongwang@hyit.edu.cn;xiongaisheng@njau.edu.cn
CLC Number:
BIAN Shicun, LU Yani, XU Wujun, CHEN Boqing, WANG Guanglong, XIONG Aisheng. Garlic Circadian Clock Genes AsRVE1 and AsRVE2 and Their Expression Analysis Under Osmotic Stress[J]. Acta Horticulturae Sinica, 2021, 48(9): 1706-1716.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0961
用途 Use | 基因名称 Gene name | 引物(上游/下游) Primer(Forward/Reverse) |
---|---|---|
克隆 Gene cloning | AsRVE1 | ATGACAAGTGATCCTTTATACTTGG/TCATCTCTTGTATGGAAGAAATCCT |
AsRVE2 | ATGCAGATCAGTAGTACATCACCTG/TCACCTCCTGTACGGAACAAATCCC | |
荧光定量PCR Quantitative real time fluorescence PCR | AsRVE1 | TCAGTGGATGTGGAGAACAATACCT/TGATGAGTAAGGAGGCAACAGATGA |
AsRVE2 | ACAGCGTGAGAGGTGGACTGAT/GAGATGCAGACGGCGAACCAA | |
AsSAND | GCGTCAACGAATGTTCCAATTACCA/TCTCTTCAGTCTCAACTTCATCAGCAT |
Table 1 Primer sequences used for gene cloning and quantitative real time fluorescence PCR
用途 Use | 基因名称 Gene name | 引物(上游/下游) Primer(Forward/Reverse) |
---|---|---|
克隆 Gene cloning | AsRVE1 | ATGACAAGTGATCCTTTATACTTGG/TCATCTCTTGTATGGAAGAAATCCT |
AsRVE2 | ATGCAGATCAGTAGTACATCACCTG/TCACCTCCTGTACGGAACAAATCCC | |
荧光定量PCR Quantitative real time fluorescence PCR | AsRVE1 | TCAGTGGATGTGGAGAACAATACCT/TGATGAGTAAGGAGGCAACAGATGA |
AsRVE2 | ACAGCGTGAGAGGTGGACTGAT/GAGATGCAGACGGCGAACCAA | |
AsSAND | GCGTCAACGAATGTTCCAATTACCA/TCTCTTCAGTCTCAACTTCATCAGCAT |
Fig. 4 Alignment of RVE amino acid sequences from garlic and other plant As:Allium sativum;At:Arabidopsis thaliana;Ls:Lactuca sativa;Zm:Zea mays;Rs:Raphanus sativus;Vr:Vigna radiata;Ac:Ananas comosus. * indicates DNA binding sites.
Fig. 5 Prediction of secondary structure of AsRVE1 and AsRVE2 of garlic Blue,red,green,and purple respectively represent alpha helix,extended strand,beta turn,and random coil.
Fig. 8 Expression level of garlic AsRVE1 and AsRVE2 under drought stress Different lowercase letters in the same tissue represent significant differences at 0.05 level.
Fig. 9 Expression level of garlic AsRVE1 and AsRVE2 under salt stress Different lowercase letters in the same tissue represent significant differences at 0.05 level.
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