Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (6): 1079-1093.doi: 10.16420/j.issn.0513-353x.2020-0902

• Research Papers • Previous Articles     Next Articles

VaCBL6 from Vitis amurensis Involved in Abiotic Stress Response and ABA-mediated Pathway

MA Junjie1, SONG Lina1, LI Le1, MA Xiaochun1, JIN Lei1, XU Weirong2,3,4,*()   

  1. 1College of Agronomy,Ningxia University,Yinchuan 750021,China
    2School of Food & Wine,Ningxia University,Yinchuan 750021,China
    3China Wine Industry Technology Institute,Yinchuan 750021,China
    4Key Laboratory of Modern Molecular Breeding of Dominant and Special Crops in Ningxia,Yinchuan 750021,China
  • Received:2021-03-16 Revised:2021-05-11 Online:2021-06-25 Published:2021-07-07
  • Contact: XU Weirong


A calcineurin B-like phosphatase subunit protein gene VaCBL6 from Vitis amurensis ‘Zuoshan-1'was cloned and functionally analyzed. VaCBL6is located on chromosome 4 and has an open reading frame of 777 bp,encoding 258 aa;protein domain analysis shows that the protein contains four EF-hands and one transmembrane domain. Phylogenetic analysis using amino acid sequence showed that high similarities between VaCBL6 and VvCBL9,VaCBL6 and VvCBL6 are 98.84% and 94.96%,respectively. VaCBL6 is located in the nucleus and cell membrane,and is highly expressed in roots and tendrils,and its expression is induced by low temperature,drought,NaCl and ABA. Analysis of transcriptional activation based on the yeast system displayed that VaCBL6 had no transcriptional activation activity. Experiments on the growth stage ofArabidopsis seedlings overexpressingVaCBL6with different treatments found that 100-125 μmol · L -1 NaCl and 0.25-1.25 μmol · L -1 ABA treatments inhibited the germination of VaCBL6 transgenic Arabidopsis seeds,and the transgenic Arabidopsis seedlings increased the sensitivity to NaCl and ABA. The results indicated that VaCBL6 plays an important role in plant stress response.

Key words: Vitis amurensis, calcineurin B-like phosphatase subunit protein, gene cloning, abiotic stress, functional analysis

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