Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1121-1135.doi: 10.16420/j.issn.0513-353x.2021-0092

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Genomic Identification and Molecular Evolution of the WRKY Family in Nymphaea colorata

PAN Xinfeng, YE Fangting, MAO Zhijun, LI Zhaowei, FAN Kai()   

  1. Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilzation of Crops,College of Agriculture,Fujian Agriculture And Forestry University,Fuzhou 350002,China
  • Received:2021-12-06 Revised:2022-02-11 Online:2022-05-25 Published:2022-05-25
  • Contact: FAN Kai


Sixty-nine NcWRKY transcription factors were identified in Nymphaea colorata and can be divided into three large subfamilies(I,Ⅱ and Ⅲ). The Ⅱ subfamily can be further classified into Ⅱa,Ⅱb,Ⅱc,Ⅱd and Ⅱe. The Ⅱc as subfamily has the highest number of NcWRKY members with 17 members. Random coil accounted for 39%-78% in the protein secondary structure of the NcWRKY members. Motif 1 and motif 3 were annotated as WRKY conserved domain. NcWRKY members were distributed in waterlily 14 chromosomes. All members of the Ⅱa subfamily were located on chromosome Nc4. NcWRKY members can be regulated by hormones through the cis-element analysis of their promoters. NcWRKY04 and NcWRKY18 had very high expression level in mature leafstalk,NcWRKY05 had very high expression level in flower and NcWRKY61 had very high expression level in stamen through tissue specific expression analysis. NcWRKY61 and NcWRKY63 may have a co-expression relationship with other members through the gene expression network.

Key words: Nymphaea colorata, WRKY, genomic identification, molecular evolution

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