Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1713-1722.doi: 10.16420/j.issn.0513-353x.2021-0569

• Research Papers • Previous Articles     Next Articles

Mir396b Negatively Regulates Eggplant Defense Response to Verticillium Wilt

LIN Yuanmi, ZHU Wenjiao, CHEN Min, XUE Chunmei, JIN Fangyu, ZHU Yuping, JIANG Xinyue, YE Lingfeng, NI Shunanling, YANG Qing*()   

  1. College of Life Science,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-12-27 Revised:2022-03-07 Online:2022-08-25 Published:2022-09-05
  • Contact: YANG Qing


In order to identify the function of miR396b in eggplant resistance to verticillium wilt,pri-miR396b gene was cloned from eggplant cultivar‘Suqi 1’and miR396b transgenic eggplant lines were obtained through Agrobacterium-mediated transformation. In infected eggplant,the expression level of miR396b was down-regulated by Verticillium dahliae induction. The disease resistance analysis showed that miR396b overexpression line was more sensitive to Verticillium dahliae infection compared with the control with disease index of 75.2,while miR396b antisense lines showed more resistance with disease index of 16.2-25.3. ITS quantitative analysis showed that the content of Verticillium dahliae in the vascular tissues of miR396b overexpression plants was significantly increased compared with the wild- type eggplant,while that in miR396b antisense plants was significantly reduced. Antioxidant enzyme activity analysis showed that the activities of SOD,POD and CAT in miR396b overexpression line were lower than those in the wild-type eggplant,while they were higher in antisense lines than those in the wild-type eggplant. These results showed that miR396b might play a negative role in eggplant defense response against Verticillium wilt.

Key words: eggplant, miR396b, verticillium wilt, functional analysis

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