Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (10): 1934-1944.doi: 10.16420/j.issn.0513-353x.2021-0613
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ZOU Hongzhu, ZHOU Lin, HAN Lulu, LÜ Jihang, WANG Yan*()
Received:
2021-07-28
Revised:
2021-09-09
Online:
2021-10-25
Published:
2021-11-01
Contact:
WANG Yan
E-mail:chwy8915@sina.com
CLC Number:
ZOU Hongzhu, ZHOU Lin, HAN Lulu, LÜ Jihang, WANG Yan. Changes of Carotenoid Components and Expression of the Related Genes During Petal Coloring of Paeonia delavayi[J]. Acta Horticulturae Sinica, 2021, 48(10): 1934-1944.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0613
分类 Classification | 标准品 Standard | 线性方程 Equation | 相关系数 Coefficient of correlation |
---|---|---|---|
胡萝卜素 | α-胡萝卜素α-Carotene | y = 4.98584e5x-1108.06123 | 0.99999 |
Carotene | 番茄红素Lycopene | y = 3.49839e5x-242.82230 | 0.99982 |
γ-胡萝卜素γ-Carotene | y = 4.37283e5x-547.23525 | 0.99998 | |
β-胡萝卜素β-Carotene | y = 5.74793e5x-11445.03070 | 0.99999 | |
六氢番茄红素Phytofluene | y = 1.42297e5x + 23435.01369 | 0.99995 | |
八氢番茄红素(E/Z)-Phytoene | y = 7.11827e4x-567.43881 | 1.00000 | |
ε-胡萝卜素ε-Carotene | y = 1.16042e6x + 1186.48166 | 0.99980 | |
叶黄素 | 花药黄质Antheraxanthin | y = 5.55136e5x-1297.82785 | 1.00000 |
Lutein | 玉米黄质Zeaxanthin | y = 1.82585e5x + 9928.65201 | 0.99985 |
紫黄质Violaxanthin | y = 1.47435e6x-3.30834e4 | 0.99994 | |
新黄质Neoxanthin | y = 1.22081e6x-21272.87715 | 0.99928 | |
叶黄素Lutein | y = 9.00512e5x + 1.17150e5 | 0.99998 | |
β-隐黄质β-Cryptoxanthin | y = 2.29006e5x + 5179.02939 | 0.99998 | |
虾青素Astaxanthin | y = 4.24452e6x + 1051.48536 | 0.99668 | |
β-阿朴胡萝卜素醛Apocarotenal | y = 8.55527e6x-1234.24238 | 1.00000 | |
辣椒红素Capsanthin | y = 1.11119e7x + 4273.93502 | 0.99901 | |
α-隐黄质α-Cryptoxanthin | y = 5.99746e5x + 388.44092 | 0.99988 | |
辣椒玉红素Capsorubin | y = 2.33996e6x-1575.19663 | 0.99991 |
Table 1 Regression analysis of carotenoid standards
分类 Classification | 标准品 Standard | 线性方程 Equation | 相关系数 Coefficient of correlation |
---|---|---|---|
胡萝卜素 | α-胡萝卜素α-Carotene | y = 4.98584e5x-1108.06123 | 0.99999 |
Carotene | 番茄红素Lycopene | y = 3.49839e5x-242.82230 | 0.99982 |
γ-胡萝卜素γ-Carotene | y = 4.37283e5x-547.23525 | 0.99998 | |
β-胡萝卜素β-Carotene | y = 5.74793e5x-11445.03070 | 0.99999 | |
六氢番茄红素Phytofluene | y = 1.42297e5x + 23435.01369 | 0.99995 | |
八氢番茄红素(E/Z)-Phytoene | y = 7.11827e4x-567.43881 | 1.00000 | |
ε-胡萝卜素ε-Carotene | y = 1.16042e6x + 1186.48166 | 0.99980 | |
叶黄素 | 花药黄质Antheraxanthin | y = 5.55136e5x-1297.82785 | 1.00000 |
Lutein | 玉米黄质Zeaxanthin | y = 1.82585e5x + 9928.65201 | 0.99985 |
紫黄质Violaxanthin | y = 1.47435e6x-3.30834e4 | 0.99994 | |
新黄质Neoxanthin | y = 1.22081e6x-21272.87715 | 0.99928 | |
叶黄素Lutein | y = 9.00512e5x + 1.17150e5 | 0.99998 | |
β-隐黄质β-Cryptoxanthin | y = 2.29006e5x + 5179.02939 | 0.99998 | |
虾青素Astaxanthin | y = 4.24452e6x + 1051.48536 | 0.99668 | |
β-阿朴胡萝卜素醛Apocarotenal | y = 8.55527e6x-1234.24238 | 1.00000 | |
辣椒红素Capsanthin | y = 1.11119e7x + 4273.93502 | 0.99901 | |
α-隐黄质α-Cryptoxanthin | y = 5.99746e5x + 388.44092 | 0.99988 | |
辣椒玉红素Capsorubin | y = 2.33996e6x-1575.19663 | 0.99991 |
基因 Gene | 正向引物(5′-3′) Forword primer | 反向引物(5′-3′) Reverse primer |
---|---|---|
PdPSY | ACACCGGCAGCTTTGGATAGGT | AGCCAACAGTTCCAGCGACA |
PdCRISTO | TGATGCCGAGGGAATACGCA | TCAAATGGCTCGGTTCCTGGTG |
PdCHYB | TGCTCTTTCTGTGGGTGCTGCT | ACCAGCGCCAAAACAGAGTCCA |
PdLCYB | TCGACCTGGACTCTGCATGGAA | GCCGTACCACCAATCCCAACAA |
PdCHYE | TGGTGGTTATTGGCTGTGGTCCA | CCGCCAAACATGCTCAATACACCC |
PdNXS | TATTGGTGGCACGGCTGGGTTA | ATAGGCCACAACCCGTTCCACA |
PdZDS | AAGGAGCCAGATTTCGCGGGTT | TTGACATGCCTGCAAGCCCA |
PdCCD | GGTTGGCCGGAAACTTTGCT | ACCATGCCATCGCCATCAAACC |
PsPP2A | CGTGTTTGGATGTTCTCAAGGC | GGCGAGTGAGTTTTCAGTTGGA |
Table 2 Primers used for real-time quantitative RT-PCR
基因 Gene | 正向引物(5′-3′) Forword primer | 反向引物(5′-3′) Reverse primer |
---|---|---|
PdPSY | ACACCGGCAGCTTTGGATAGGT | AGCCAACAGTTCCAGCGACA |
PdCRISTO | TGATGCCGAGGGAATACGCA | TCAAATGGCTCGGTTCCTGGTG |
PdCHYB | TGCTCTTTCTGTGGGTGCTGCT | ACCAGCGCCAAAACAGAGTCCA |
PdLCYB | TCGACCTGGACTCTGCATGGAA | GCCGTACCACCAATCCCAACAA |
PdCHYE | TGGTGGTTATTGGCTGTGGTCCA | CCGCCAAACATGCTCAATACACCC |
PdNXS | TATTGGTGGCACGGCTGGGTTA | ATAGGCCACAACCCGTTCCACA |
PdZDS | AAGGAGCCAGATTTCGCGGGTT | TTGACATGCCTGCAAGCCCA |
PdCCD | GGTTGGCCGGAAACTTTGCT | ACCATGCCATCGCCATCAAACC |
PsPP2A | CGTGTTTGGATGTTCTCAAGGC | GGCGAGTGAGTTTTCAGTTGGA |
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