Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1758-1772.doi: 10.16420/j.issn.0513-353x.2024-0116
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
HAN Ying1,2, DUAN Ying2, NIU Yijie2, LI Yansu2, HE Chaoxing2, SUN Mintao2, WANG Jun2, LI Qiang3,**(), CHEN Shuangchen1,**(
), YAN Yan2,**(
)
Received:
2024-03-20
Revised:
2024-06-19
Online:
2024-08-25
Published:
2024-08-21
Contact:
LI Qiang, CHEN Shuangchen, YAN Yan
HAN Ying, DUAN Ying, NIU Yijie, LI Yansu, HE Chaoxing, SUN Mintao, WANG Jun, LI Qiang, CHEN Shuangchen, YAN Yan. Transcription Metabolic Mechanism of Humic Acid Biodegradable Plastic Film to Improving the Fruit Quality of Tomato in the Greenhouse[J]. Acta Horticulturae Sinica, 2024, 51(8): 1758-1772.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2024-0116
级别 Grade | 分数 Score | 风味口感评价标准 Evaluate standard of the taste in grafted tomato |
---|---|---|
1 | 9.0 ~ 10.0 | 口感甜,浓郁清香味,肉质软 Sweet taste,rich and clear fragrance,soft sarcocarp |
2 | 6.0 ~ 8.0 | 口感酸甜,有清香味,肉质软 Taste sour and sweet,clear fragrance,soft sarcocarp |
3 | 3.0 ~ 5.0 | 口感甜酸,略有清香味,肉质面 Taste sweet and sour,slightly fragrant,slightly hard sarcocarp |
4 | 0 ~ 2.0 | 口感酸,无清香味,肉质沙 Sour taste,no fragrance,hard sarcocarp |
Table 1 Standard of fruit tasty in tomato
级别 Grade | 分数 Score | 风味口感评价标准 Evaluate standard of the taste in grafted tomato |
---|---|---|
1 | 9.0 ~ 10.0 | 口感甜,浓郁清香味,肉质软 Sweet taste,rich and clear fragrance,soft sarcocarp |
2 | 6.0 ~ 8.0 | 口感酸甜,有清香味,肉质软 Taste sour and sweet,clear fragrance,soft sarcocarp |
3 | 3.0 ~ 5.0 | 口感甜酸,略有清香味,肉质面 Taste sweet and sour,slightly fragrant,slightly hard sarcocarp |
4 | 0 ~ 2.0 | 口感酸,无清香味,肉质沙 Sour taste,no fragrance,hard sarcocarp |
基因 Gene | 引物 Primer |
---|---|
Solyc09g075420 | F:5′-TATGGCCGACTGATTTCTGG-3′;R:5′-CCAAACCCTAACCCCTTTTCT-3′ |
Solyc08g079480 | F:5′-TGGTCTCCAGAAAAAGCATCTAAAG-3′;R:5′- ACCTGTATGATAATTTGCCACAGC-3′ |
Solyc08g078850 | F:5′- TAGATTTCGGTTTTTGATTGCG-3′;R:5′-CACAACTTCTTGGTGGATTTTTT-3′ |
Solyc09g011810 | F:5′-GGTGGCATTTATGGTTATCCTAGA-3′;R:5′-TCCAATTTTTCAACTTCTTCTGTG-3′ |
Solyc02g062340 | F:5′-ATTCCCCCTGCTGTCCCT-3′;R:5′-GAGCTGCCTCAACATTTTCTG-3′ |
Solyc05g055840 | F:5′-GCGGACACTTTCTTTGTCTGG-3′;R:5′-CAATAGCTTGAACGCCTGGTA-3′ |
Actin | F:5′-TGTCCCTATTTACGAGGGTTATGC-3′;R:5′-AGTTAAATCACGACCAGCAAGAT-3′ |
Table 2 qRT-PCR validation gene and primer information
基因 Gene | 引物 Primer |
---|---|
Solyc09g075420 | F:5′-TATGGCCGACTGATTTCTGG-3′;R:5′-CCAAACCCTAACCCCTTTTCT-3′ |
Solyc08g079480 | F:5′-TGGTCTCCAGAAAAAGCATCTAAAG-3′;R:5′- ACCTGTATGATAATTTGCCACAGC-3′ |
Solyc08g078850 | F:5′- TAGATTTCGGTTTTTGATTGCG-3′;R:5′-CACAACTTCTTGGTGGATTTTTT-3′ |
Solyc09g011810 | F:5′-GGTGGCATTTATGGTTATCCTAGA-3′;R:5′-TCCAATTTTTCAACTTCTTCTGTG-3′ |
Solyc02g062340 | F:5′-ATTCCCCCTGCTGTCCCT-3′;R:5′-GAGCTGCCTCAACATTTTCTG-3′ |
Solyc05g055840 | F:5′-GCGGACACTTTCTTTGTCTGG-3′;R:5′-CAATAGCTTGAACGCCTGGTA-3′ |
Actin | F:5′-TGTCCCTATTTACGAGGGTTATGC-3′;R:5′-AGTTAAATCACGACCAGCAAGAT-3′ |
Fig. 1 Fruit phenotype(A)and pericarp cell(B)of tomato covered with ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)55 d after pollination
处理 Treatment | 角质层厚度/μm Cutin thickness | 表皮层厚度/μm Epidermis thickness | 表皮细胞宽/μm Epidermal cell width | 薄壁细胞宽/μm Parenchyma cell width | 单位面积表皮细胞数 Number of epidermal cells per unit area | 单位面积薄壁细胞数 Number of parenchyma cells per unit area |
---|---|---|---|---|---|---|
PT | 6.59 ± 0.83 | 75.73 ± 4.68 | 8.22 ± 2.50 | 17.92 ± 6.34 | 10.21 ± 2.06 | 32.17 ± 5.97 |
FZS | 4.99 ± 0.67* | 42.42 ± 3.25* | 9.85 ± 2.42 | 15.23 ± 2.81 | 9.75 ± 1.59 | 23.13 ± 5.90* |
Table 3 Coverage of the effects of ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)on the thickness of tomato fruit epidermal tissue
处理 Treatment | 角质层厚度/μm Cutin thickness | 表皮层厚度/μm Epidermis thickness | 表皮细胞宽/μm Epidermal cell width | 薄壁细胞宽/μm Parenchyma cell width | 单位面积表皮细胞数 Number of epidermal cells per unit area | 单位面积薄壁细胞数 Number of parenchyma cells per unit area |
---|---|---|---|---|---|---|
PT | 6.59 ± 0.83 | 75.73 ± 4.68 | 8.22 ± 2.50 | 17.92 ± 6.34 | 10.21 ± 2.06 | 32.17 ± 5.97 |
FZS | 4.99 ± 0.67* | 42.42 ± 3.25* | 9.85 ± 2.42 | 15.23 ± 2.81 | 9.75 ± 1.59 | 23.13 ± 5.90* |
处理 Treatment | 硬度 Hardness | 甜度 Sweetness | 酸度 Acidity | 风味 Flavor |
---|---|---|---|---|
PT | 8.30 ± 0.90 | 6.10 ± 1.13 | 7.50 ± 0.78 | 6.40 ± 1.28 |
FZS | 5.50 ± 0.92* | 7.30 ± 0.90* | 5.90 ± 0.70* | 8.00 ± 0.88* |
Table 4 Flavor and taste scores of mature tomato fruits covered with ordinary biodegradable film(PT) and humic acid biodegradable film(FZS)
处理 Treatment | 硬度 Hardness | 甜度 Sweetness | 酸度 Acidity | 风味 Flavor |
---|---|---|---|---|
PT | 8.30 ± 0.90 | 6.10 ± 1.13 | 7.50 ± 0.78 | 6.40 ± 1.28 |
FZS | 5.50 ± 0.92* | 7.30 ± 0.90* | 5.90 ± 0.70* | 8.00 ± 0.88* |
处理 Treatment | 果实硬度/(kg · cm-2) Fruit firmness | 维生素C/(mg · kg-1) Vitamin C | 可溶性糖/% Soluble sugar | 可溶性固形物/% Soluble solids | 番茄红素/(μg · g-1) Lycopene | 可滴定酸/% Titratable acid | 糖酸比 Sugar acid ratio |
---|---|---|---|---|---|---|---|
PT | 10.50 ± 0.31 | 1.48 ± 1.08 | 2.47 ± 0.03 | 7.83 ± 0.12 | 79.32 ± 2.81 | 5.81 ± 0.01 | 42.48 ± 0.64 |
FZS | 9.80 ± 0.13* | 1.40 ± 0.17 | 2.71 ± 0.01* | 8.17 ± 0.06* | 90.39 ± 5.49* | 5.64 ± 0.07* | 48.09 ± 0.78* |
Table 5 Effects of ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)on tomato fruit quality
处理 Treatment | 果实硬度/(kg · cm-2) Fruit firmness | 维生素C/(mg · kg-1) Vitamin C | 可溶性糖/% Soluble sugar | 可溶性固形物/% Soluble solids | 番茄红素/(μg · g-1) Lycopene | 可滴定酸/% Titratable acid | 糖酸比 Sugar acid ratio |
---|---|---|---|---|---|---|---|
PT | 10.50 ± 0.31 | 1.48 ± 1.08 | 2.47 ± 0.03 | 7.83 ± 0.12 | 79.32 ± 2.81 | 5.81 ± 0.01 | 42.48 ± 0.64 |
FZS | 9.80 ± 0.13* | 1.40 ± 0.17 | 2.71 ± 0.01* | 8.17 ± 0.06* | 90.39 ± 5.49* | 5.64 ± 0.07* | 48.09 ± 0.78* |
Fig. 2 Histogram and volcanic map of differentially expressed genes in tomato fruits covered with ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)
Fig. 3 Enrichment of go function of differentially expressed genes in tomato covered with ordinary biodegradable film(PT) and humic acid biodegradable film(FZS)
Fig. 4 KEGG functional enrichment of differentially expressed genes in tomato covered with ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)
Fig.6 Clustering and KEGG enrichment of differential metabolites in tomato fruits covered with ordinary biodegradable film(PT) and humic acid biodegradable film(FZS)
Fig. 7 Common mapping pathways of transcription metabolism poor foreign bodies in tomato covered with ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)
Fig. 8 Main common mapping pathways of transcription metabolism poor foreign bodies in tomato covered with ordinary biodegradable film(PT)and humic acid biodegradable film(FZS)
Fig. 9 qRT-PCR verification of tomato related gene expression levels covered with ordinary biodegradable film(PT) and humic acid biodegradable film(FZS)
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