Molecular Cloning and Tolerance Identification of Apple Cytokinin Oxidase Gene MdCKX7.2

doi:10.16420/j.issn.0513-353x.2018-0578

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (3): 409-420.doi: 10.16420/j.issn.0513-353x.2018-0578

Molecular Cloning and Tolerance Identification of Apple Cytokinin Oxidase Gene MdCKX7.2

YAO Jifang，AN Jianping，YOU Chunxiang，WANG Xiaofei*，and HAO Yujin*

（College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China）

Online:2019-03-25 Published:2019-03-25

Abstract

Abstract: MdCKX7.2 gene（MDP0000279125）was cloned from Malus × domestica Borkh‘Gala’using homologous cloning and RT-PCR. The gene contained a 1 542 bp complete open reading frame（ORF）and encoded 513 amino acids. The cis-acting elements of the MdCKX7.2 promoter were analyzed using the Plant CARE database. It was found that there were elements including light，drought，abscisic acid，salicylic acid，and methyl jasmonate in the MdCKX7.2 promoter sequence. Expression analysis revealed that the transcription of MdCKX7.2 in‘Gala’seedlings was significantly induced by drought and ABA treatments. MdCKX7.2 transgenic Arabidopsis plants were obtained by Agrobacterium-mediated genetic transformation. The stress tolerance assays showed that ectopic expression of MdCKX7.2 significantly increased the tolerance of transgenic Arabidopsis to drought stress. Arabidopsis seed germination experiments showed that ectopic expression of MdCKX7.2 in Arabidopsis enhanced the sensitivity of Arabidopsis to ABA，the seed germination rate and seedling fresh weight decreased significantly，and the expression level of genes related to seed germination was significantly up-regulated. The above results indicate that MdCKX7.2 plays an important role in plant abiotic stress response.

Key words: apple, MdCKX7.2, gene clone, abiotic stress, drought tolerance, ABA sensitivity

CLC Number:

S 661.1

YAO Jifang，AN Jianping，YOU Chunxiang，WANG Xiaofei*，and HAO Yujin*. Molecular Cloning and Tolerance Identification of Apple Cytokinin Oxidase Gene MdCKX7.2[J]. ACTA HORTICULTURAE SINICA, 2019, 46(3): 409-420.

[1]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[2]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YANG An, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Zhongping Youlei’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 1-2.
[3]	QU Yanting, XIONG Yan, LIU Zhiyang, HAN Hui, CHEN Fei, LI Li, and ZHANG Xing, . A New Hosta Cultivar‘Nature Green’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 197-198.
[4]	FAN Jinping, DAI Jingqi, JIANG Yifan, YAN Kaili, ZHANG Jinzhu, YANG Tao, and CHE Daidi. A New Lilium Cultivar‘Ice Pink Queen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 205-206.
[5]	SUN Simiao, WANG Kun, GAO Yuan, WANG Dajiang, and LI Lianwen. A New Ornamental Crabapple Cultivar‘Zichen’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 267-268.
[6]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Pingyou 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 1-2.
[7]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Late Ripening Apple Cultivar‘Huayou Tianwa’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 3-4.
[8]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Mid-ripening Apple Cultivar‘Huayou Baomi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 5-6.
[9]	YANG Ling, CONG Peihua, WANG Qiang, LI Wuxing, and KANG Liqun. A New Mid-ripening Apple Cultivar‘Huafeng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 7-8.
[10]	XU Xiaoping, CAO Qingying, CAI Roudi, GUAN Qingxu, ZHANG Zihao, CHEN Yukun, XU HAN, LIN Yuling, LAI Zhongxiong. Gene Cloning and Expression Analysis of miR408 and Its Target DlLAC12 in Globular Embryo Development and Abiotic Stress in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(9): 1866-1882.
[11]	JIA Xin, ZENG Zhen, CHEN Yue, FENG Hui, LÜ Yingmin, ZHAO Shiwei. Cloning and Expression Analysis of RcDREB2A Gene in Rosa chinensis‘Old Blush’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 1945-1956.
[12]	LIU Chuanhe, HE Han, SHAO Xuehua, LAI Duo, KUANG Shizi, XIAO Weiqiang, LIU Yan. A New Pineapple Cultivar‘Yuetong’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 2053-2054.
[13]	GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu. Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2022, 49(8): 1621-1636.
[14]	LIU Chaoyang, LIAO Zhichan, LU Xinxin, HE Yehua. Identification of CslD Gene Family in Pineapple and Functional Analysis of AcoCslD2a [J]. Acta Horticulturae Sinica, 2022, 49(8): 1650-1662.
[15]	ZHENG Xiaodong, XI Xiangli, LI Yuqi, SUN Zhijuan, MA Changqing, HAN Mingsan, LI Shaoxuan, TIAN Yike, WANG Caihong. Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress [J]. Acta Horticulturae Sinica, 2022, 49(7): 1401-1414.

Molecular Cloning and Tolerance Identification of Apple Cytokinin Oxidase Gene MdCKX7.2

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments