PCR Assay for Detection of Corynespora cassiicola，the Causal Agent of Corynespora Leaf Spot of Cucumber

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (3): 585-592.

• Technologies and Methods • Previous Articles Next Articles

PCR Assay for Detection of Corynespora cassiicola，the Causal Agent of Corynespora Leaf Spot of Cucumber

CHEN Lu*，SHI Yan-xia*，XIE Xue-wen，CHAI A-li，and LI Bao-ju**

Invstitute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Online:2014-03-25 Published:2014-03-25

Abstract

Abstract: A rapid PCR detection assay of Corynespora cassiicola，the causal agent of Corynespora
leaf spot of cucumber，was developed based on the Actin gene of the pathogen. Primer pair Caa5F/Caa5R
were designed on the different bases of Actin gene sequences between C. cassiicola and other common
plant fungal pathogens，including Corynespora lieustri and Corynespora cambrensis，and only C.
cassiicola performed a 160 bp amplication. C. cassiicola could be detected in infected cucumber leaves
without isolation or cultivation by the PCR assay. The assay with a high sensitivity of 4 pg · μL-1 DNA per
reaction could be a useful tool of rapid detection of Corynespora leaf spot of cucumber.

Key words: Corynespora cassiicola, Actin gene, PCR

CLC Number:

S 642.2

CHEN Lu, SHI Yan-Xia, XIE Xue-Wen, CHAI A-Li, LI Bao-Ju. PCR Assay for Detection of Corynespora cassiicola，the Causal Agent of Corynespora Leaf Spot of Cucumber[J]. ACTA HORTICULTURAE SINICA, 2014, 41(3): 585-592.

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PCR Assay for Detection of Corynespora cassiicola，the Causal Agent of Corynespora Leaf Spot of Cucumber

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