Abstract: Abstract：In this research，we analyzed the asymmetrical somatic hybrids of cauliflower（Brassica
oleracea var. botrytis，CC，2n = 18）and black mustard（B. nigra，BB，2n = 16）and their selfed or
backcrossed progenies by integrating morphological，cytological and molecular information．Among 16
advanced selfing and backcrossing descendants in analysis，12 introgression lines displaying the
cauliflower-like morphology were identified with chromosome number 18. Simple sequence repeat（SSR）
and amplified fragment length polymorphism（AFLP）analysis showed that those introgression lines
preserved some DNA banding patterns from B. nigra，B. carinata（BBCC，2n = 34）and some of new
bands，and all of these introgression lines showed an absence of some cauliflower-specific sites. 4 plants
（PFCN29BC2S1-4，PFCN15-2S1BC5-6-2，PFCN29BC4-37-7 and PFCN29BC4-38-5）were confirmed to
have 9，16，16 and 18 cauliflower chromosomes added with 8，7，14 and 5–7 B. nigra-derived chromosomes respectively by fluorescence in situ hybridization（FISH）.

Key words: cauliflower, Brassica nigra, introgression lines, SSR, AFLP, FISH