Cloning and Function Analysis of Part of PGIP Gene Family Member Promoters from Apple

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (11): 2169-2178.

Cloning and Function Analysis of Part of PGIP Gene Family Member Promoters from Apple

FU Cong-hui，WANG Jian-ping，ZHANG Chong，MA Feng-wang，and ZHANG Jun-ke

College of Horticulture，Northwest A & F University，Yangling，Shaanxi 712100，China

Received:2014-06-27 Online:2014-11-25 Published:2014-11-25

Abstract

Abstract: Primers were designed according to the published sequence of the apple genome and two PGIP gene promoters of Malus× domestica Borkh.‘Qinguan’and‘Pacific Rose’were cloned. PGIP1 were 71% homology with PGIP2 gene promoters in both apple cultivar；Both PGIP1 and PGIP2 gene promoters of‘Qinguan’and‘Pacific Rose’shared a sequence identity of 99%. PGIP1 promoters had more TATA-box and CAAT-box than that of PGIP2 promoters，and PGIP1 gene promoters contained methyl jasmonate-responsive elements，while PGIP2 gene promoters owed salicylic acid responsive elements. It’s speculated that there are different disease response pathways of PGIP1 and PGIP2. PGIP promoter activity were evaluated by promoter︰︰GUS fusion expression in transgenic tobacco leaves and the results showed that the PGIP1 promoters activity was not significant between cultivars while PGIP2 promoter of‘Qinguan’was higher than that of‘Pacific Rose’，and the former’s activity was 2.37 folds of the latter，which was probably related to the resistance difference. In the same apple cultivar，PGIP1 gene promoters’activities were significantly higher than that of PGIP2.

Key words: apple, PGIP gene, promoter, cis-acting element

CLC Number:

S 661.1

FU Cong-Hui, WANG Jian-Ping, ZHANG , CHONG , MA Feng-Wang, ZHANG Jun-Ke. Cloning and Function Analysis of Part of PGIP Gene Family Member Promoters from Apple[J]. ACTA HORTICULTURAE SINICA, 2014, 41(11): 2169-2178.

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Cloning and Function Analysis of Part of PGIP Gene Family Member Promoters from Apple

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