Cloning，Expression Analysis of MdGID1a Gene and Promoter from Spur-type Apple

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (11): 2237-2244.

• Research Notes • Previous Articles Next Articles

Cloning，Expression Analysis of MdGID1a Gene and Promoter from Spur-type Apple

SONG Yang1,2，ZHANG Yan-min2，WU Shu-jing2，FENG Shou-qian2，WANG Chuan-zeng3，and CHEN
Xue-sen2,*

1Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（Germplasm Resources Utilization），
Ministry of Agriculture，P. R. China，Research Institute of Pomology，Chinese Academy of Agricultural Sciences，Xingcheng，
Liaoning 125100，China；2College of Horticulture Science and Engineering，Shandong Agricultural University/State Key
Laboratory of Crop Biology，Tai’an，Shandong 271018，China； 3 Shandong Institute of Pomology，Tai’an，Shandong 271000，
China

Online:2013-11-25 Published:2013-11-25

Abstract

Abstract: MdGID1a gene was amplified from spur-type apple（Malus × domestica Borkh.‘Longfu
Duanzhi’）shoots by using RT-PCR and RACE method. The sequence has been deposited in GenBank
database with the accession number of JF516247. The gene was named MdGID1a，containing an open
reading frame（1 035 bp）and encoding a protein of 345 amino acid. Sequence analysis indicated that
MdGID1a shared highly homology with HGG and GXSXG domain of HSL family gene. The promoter of
MdGID1a contained phytohormone and light regulatory elements. The real-time quantitative PCR analysis
showed that the expression levels of MdGID1a gene changed in different tissues，standard shoots andshoots of different growth stages. The GA receptor gene MdGID1a may play an important role during the
shoots development apple.

Key words: apple, GA receptor gene, gene expression analysis, promoter

CLC Number:

S 661.1

SONG Yang, ZHANG Yan-Min, WU Shu-Jing, FENG Shou-Qian, WANG Chuan-Zeng, CHEN Xue-Sen. Cloning，Expression Analysis of MdGID1a Gene and Promoter from Spur-type Apple[J]. ACTA HORTICULTURAE SINICA, 2013, 40(11): 2237-2244.

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Cloning，Expression Analysis of MdGID1a Gene and Promoter from Spur-type Apple

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