Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (11): 2133-2141.

• Fruit Trees • Previous Articles Next Articles

Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube

WANG Si-Qi, TANG Shi-Zhe, KONG De-Cang, HE Run-Ping, LIU Hua-Bo, MA Li-Ying, LIU Jun, WANG Zhe, LI Ying-Yue, SHEN Lian-Ying, PANG Xiao-Ming

（1National Engineering Laboratory for Tree Breeding，Key Laboratory of Genetics and Breeding in Forest Trees and
Ornamental Plants，Ministry of Education, Center for Computational Biology，College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，China；2National Key Forestry Station for Improved Jujube Cultivars，Cangzhou，Hebei 061001，China；3Institute of Plant Protection；Shanxi Academy of Agricultural Sciences，Taiyuan 030031，China；4Research center of Chinese Jujube，Agricultural University of Hebei，Baoding，Hebei 071001，China）

Online:2012-11-25 Published:2012-11-25

Abstract

Abstract: The paternalparents of 1 728 open-pollinated seedlings of‘Dongzao’（Ziziphus jujuba Mill.）and 530 open-pollinated seedlings of‘Lingbao Dazao’were identified with SSR technique. Eight primer pairs were selected from 376 primer pairs，which amplified distinct，polymorphic segments and showed high differential rate. Twelve candidate parents were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method. Totally，45 polymorphic alleles were revealed，with an average of 5.625 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.58 to 0.86，with an average of 0.71. Using software Cervus3.0，399 hybrids from‘Dongzao’×‘Muzao’were identified，and 390 from‘Dongzao’×‘Xiaoyazao’，357 from‘Dongzao’×‘Yingshanhong’，204 from ‘Lingbao Dazao’×‘Ziyuanzao（Zizao）’，126 from‘Lingbao Dazao’×‘Shibingzao’were also identified.

Key words: jujube, SSR, paternal parent analysis, capillary electrophoresis

CLC Number:

S 665.1

WANG Si-Qi, TANG Shi-Zhe, KONG De-Cang, HE Run-Ping, LIU Hua-Bo, MA Li-Ying, LIU Jun, WANG Zhe, LI Ying-Yue, SHEN Lian-Ying, PANG Xiao-Ming. Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube[J]. ACTA HORTICULTURAE SINICA, 2012, 39(11): 2133-2141.

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Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube

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