The Analyses of Target Metabolomics in Flavonoid and Its Potential MYB Regulation Factors During Coloring Period of Winter Jujube

doi:10.16420/j.issn.0513-353x.2021-0326

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1761-1771.doi: 10.16420/j.issn.0513-353x.2021-0326

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The Analyses of Target Metabolomics in Flavonoid and Its Potential MYB Regulation Factors During Coloring Period of Winter Jujube

XU Haifeng¹, WANG Zhongtang¹, CHEN Xin¹, LIU Zhiguo², WANG Lihu³, LIU Ping², LIU Mengjun², ZHANG Qiong¹^,^*()

1. Shandong Institute of Pomology,Tai’an,Shandong 271000,China
2. Research Center of Chinese Jujube,Hebei Agricultural University,Baoding,Hebei 071000,China
3. College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan,Hebei 056038,China

Received:2021-11-17 Revised:2022-01-07 Online:2022-08-25 Published:2022-09-05
Contact: ZHANG Qiong E-mail:Zhangqiong@shandong.cn

Abstract

Abstract:

In our study,the white（W）,semi-red（SR_R,SR_W）and full-red（R）pericarps of winter jujube were used as materials,we analyzed components and changes of flavonoids in different coloring periods using targeted metabolomics. The phylogenetic tree of MYB family in winter jujube was constructed,and the potential MYB regulators associated with flavonoid synthesis were analyzed using transcriptomic analysis. The results showed that the differential metabolites between colored and uncolored pericarps were 291（R vs W）,196（R vs SR_W）,297（SR_R vs SR_W）,and 201（SR_R vs W）,respectively. There were 98 differential metabolites in the four comparison groups. Further analysis of KEGG revealed that the shared differential metabolites of four comparison groups mainly enriched in secondary metabolites,phenylpropanoids and flavonoid biosynthetic pathways. The 126 flavonoids were detected during the coloring period of winter jujube pericarp,including thirteen anthocyanins,fifty-three flavones,twenty-eight flavonols,eight isoflavones,five proanthocyanidins and nineteen flavanols. Along with the coloration of winter jujube pericarp,three anthocyanins,three flavones and three flavonols increased significantly,while procyanidins and flavanols（catechin,epicatechin）decreased significantly. Fourteen potential MYB regulators associated with flavonoid synthesis were screened via cluster analysis of MYB family. The analyses of transcriptome and qRT-PCR showed that twelve MYB transcription factors were down-regulated with the coloration of winter jujube pericarp,while LOC107434709 and LOC107404750 had higher expression levels in colored pericarp of winter jujube. This suggested that they might play positive roles in the coloring process of winter jujube.

Key words: jujube, coloring period, flavonoid, MYB transcription factors

CLC Number:

S665.1

XU Haifeng, WANG Zhongtang, CHEN Xin, LIU Zhiguo, WANG Lihu, LIU Ping, LIU Mengjun, ZHANG Qiong. The Analyses of Target Metabolomics in Flavonoid and Its Potential MYB Regulation Factors During Coloring Period of Winter Jujube[J]. Acta Horticulturae Sinica, 2022, 49(8): 1761-1771.

Figures/Tables 5

References 45

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引物名称 Primer name	引物序列（5′-3′） Primer sequence
LOC107434532	F：CCCAAAACAACCCTGCAAC;R：AGACTAACGCTCCTTCGACCA
LOC107406469	F：AAACGGCTACCACATCCA;R：CACCAGACTTGCCCTCCA
LOC107434709	F：AAGCTTTGATCACGGTTGG;R：TTAGGTCCCATGAACTCCG
LOC107404750	F：CGGAACTTATGCTGGACAAT;R：AGAAGAGGAACCAACGCAAA
LOC107426233	F：AGTGGGAAGGTTGTGGATAG;R：CTTTTCCCAAAAATGAACAAG
LOC107407717	F：ACCCTAACGGGAAAGACGA;R：GGAGCCTGAGAACAAGATGAA
LOC107433002	F：GATAAGCATTGCAAGCCGGG;R：TCCGAACTGGCTTTAGCTGG
LOC107415546	F：CTCCCGAAGGATCACCAAGG;R：GCAGTCCCAGTCATCAACGA
LOC107423566	F：CGCTCGTCCTCAAGCTAATC;R：GAAGAAAGCTGCGGTTCCTA
LOC107429773	F：AGAGAGCGGGTACAGATGGT;R：CAGGGCCAACATGTGCTAGA
LOC107423630	F：ACACGGGGAGGTAGTGACAA;R：CCTCCAATGGATCCTCGTTA
LOC107414848	F：TAGCCATACCTCTCATTGGCAG;R：TCATAGTACTGTGTCCCCATGA
LOC107420842	F：GTTCCTCAACTTAAAGGAAATG;R：ACAACGTTCCGTAGCAGAAAC
LOC107431136	F：CAAAGGCAGCCACATCAGG;R：ACACCAGCTAGTGTTTCTCC

引物名称 Primer name	引物序列（5′-3′） Primer sequence
LOC107434532	F：CCCAAAACAACCCTGCAAC;R：AGACTAACGCTCCTTCGACCA
LOC107406469	F：AAACGGCTACCACATCCA;R：CACCAGACTTGCCCTCCA
LOC107434709	F：AAGCTTTGATCACGGTTGG;R：TTAGGTCCCATGAACTCCG
LOC107404750	F：CGGAACTTATGCTGGACAAT;R：AGAAGAGGAACCAACGCAAA
LOC107426233	F：AGTGGGAAGGTTGTGGATAG;R：CTTTTCCCAAAAATGAACAAG
LOC107407717	F：ACCCTAACGGGAAAGACGA;R：GGAGCCTGAGAACAAGATGAA
LOC107433002	F：GATAAGCATTGCAAGCCGGG;R：TCCGAACTGGCTTTAGCTGG
LOC107415546	F：CTCCCGAAGGATCACCAAGG;R：GCAGTCCCAGTCATCAACGA
LOC107423566	F：CGCTCGTCCTCAAGCTAATC;R：GAAGAAAGCTGCGGTTCCTA
LOC107429773	F：AGAGAGCGGGTACAGATGGT;R：CAGGGCCAACATGTGCTAGA
LOC107423630	F：ACACGGGGAGGTAGTGACAA;R：CCTCCAATGGATCCTCGTTA
LOC107414848	F：TAGCCATACCTCTCATTGGCAG;R：TCATAGTACTGTGTCCCCATGA
LOC107420842	F：GTTCCTCAACTTAAAGGAAATG;R：ACAACGTTCCGTAGCAGAAAC
LOC107431136	F：CAAAGGCAGCCACATCAGG;R：ACACCAGCTAGTGTTTCTCC

类黄酮 Flavonoid	差异倍数 Log₂ fold change
类黄酮 Flavonoid	SR_R vs W R vs W SR_R vs SR_W R vs SR_W
花青苷成分 Anthocyanin component 花翠素Delphinidin	—	—	—	1.32
花翠素 3-O-葡萄糖苷Delphinidin 3-O-glucoside	—	—	—	1.13
二甲花翠素 3-O-葡萄糖苷Malvidin 3-O-glucoside	1.33	1.04	—	—
花翠素 3-O-芸香糖苷Delphinidin 3-O-rutinoside	—	—	—	—
矢车菊素Cyanidin	—	—	—	—
矢车菊素 3-O-葡萄糖苷Cyanidin 3-O-glucoside	-1.38	—	-1.35	—
矢车菊素 3,5-O-二葡萄糖苷Cyanidin 3,5-O-diglucoside	—	—	—	—
矢车菊素 3-O-丙二酰己糖苷Cyanidin 3-O-malonylhexoside	-1.68	-1.64	—	—
矢车菊素半乳糖苷Cyanidin 3-O-galactoside	—	—	-1.28	—
芍药花青素Peonidin	—	—	—	—
芍药花青素 O-己糖苷Peonidin O-hexoside	—	—	—	—
芍药-3-O-葡萄糖甙氯化物Peonidin 3-O-glucoside chloride	—	—	—	—
天竺葵色素苷Pelargonin	—	—	—	—
黄酮/黄酮醇成分 Flavone/flavonol component 8-C-己糖苷-木犀草素 O-己糖苷 8-C-hexosyl-luteolin O-hexoside	1.35	1.48	—	—
C-己糖基-木犀草素 C-戊糖苷 C-hexosyl-luteolin C-pentoside	1.46	1.48	—	—
木犀草素Luteolin	1.20	1.02	1.41	1.23
异鼠李素 5-O-己糖苷Isorhamnetin 5-O-hexoside	2.12	3.56	2.17	3.60
异鼠李素 O-己糖苷Isorhamnetin O-hexoside	2.25	3.52	2.21	3.49
异鼠李素-3-O-葡萄糖苷Isorhamnetin 3-O-glucoside	2.25	3.48	—	—
原花青素/黄烷醇成分 Procyanidin/Flavanol component 原花青素A1 Procyanidin A1	—	—	-2.96	-3.97
原花青素A2 Procyanidin A2	—	1.25	—	—
原花青素A3 Procyanidin A3	1.18	1.74	-1.38	—
原花青素B2 Procyanidin B2	-1.16	-1.57	—	-1.13
原花青素B3 Procyanidin B3	-1.01	-1.52	—	-1.21
儿茶素Catechin	-2.67	-3.91	-1.61	-2.85
表儿茶素 L-Epicatechin	-1.61	-3.43	-1.13	-2.95

类黄酮 Flavonoid	差异倍数 Log₂ fold change
类黄酮 Flavonoid	SR_R vs W R vs W SR_R vs SR_W R vs SR_W
花青苷成分 Anthocyanin component 花翠素Delphinidin	—	—	—	1.32
花翠素 3-O-葡萄糖苷Delphinidin 3-O-glucoside	—	—	—	1.13
二甲花翠素 3-O-葡萄糖苷Malvidin 3-O-glucoside	1.33	1.04	—	—
花翠素 3-O-芸香糖苷Delphinidin 3-O-rutinoside	—	—	—	—
矢车菊素Cyanidin	—	—	—	—
矢车菊素 3-O-葡萄糖苷Cyanidin 3-O-glucoside	-1.38	—	-1.35	—
矢车菊素 3,5-O-二葡萄糖苷Cyanidin 3,5-O-diglucoside	—	—	—	—
矢车菊素 3-O-丙二酰己糖苷Cyanidin 3-O-malonylhexoside	-1.68	-1.64	—	—
矢车菊素半乳糖苷Cyanidin 3-O-galactoside	—	—	-1.28	—
芍药花青素Peonidin	—	—	—	—
芍药花青素 O-己糖苷Peonidin O-hexoside	—	—	—	—
芍药-3-O-葡萄糖甙氯化物Peonidin 3-O-glucoside chloride	—	—	—	—
天竺葵色素苷Pelargonin	—	—	—	—
黄酮/黄酮醇成分 Flavone/flavonol component 8-C-己糖苷-木犀草素 O-己糖苷 8-C-hexosyl-luteolin O-hexoside	1.35	1.48	—	—
C-己糖基-木犀草素 C-戊糖苷 C-hexosyl-luteolin C-pentoside	1.46	1.48	—	—
木犀草素Luteolin	1.20	1.02	1.41	1.23
异鼠李素 5-O-己糖苷Isorhamnetin 5-O-hexoside	2.12	3.56	2.17	3.60
异鼠李素 O-己糖苷Isorhamnetin O-hexoside	2.25	3.52	2.21	3.49
异鼠李素-3-O-葡萄糖苷Isorhamnetin 3-O-glucoside	2.25	3.48	—	—
原花青素/黄烷醇成分 Procyanidin/Flavanol component 原花青素A1 Procyanidin A1	—	—	-2.96	-3.97
原花青素A2 Procyanidin A2	—	1.25	—	—
原花青素A3 Procyanidin A3	1.18	1.74	-1.38	—
原花青素B2 Procyanidin B2	-1.16	-1.57	—	-1.13
原花青素B3 Procyanidin B3	-1.01	-1.52	—	-1.21
儿茶素Catechin	-2.67	-3.91	-1.61	-2.85
表儿茶素 L-Epicatechin	-1.61	-3.43	-1.13	-2.95

The Analyses of Target Metabolomics in Flavonoid and Its Potential MYB Regulation Factors During Coloring Period of Winter Jujube

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