Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (6): 1104-1110.

• Vegetables • Previous Articles Next Articles

Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber

WANG Wei，XU Yue-jin*，and WAN Zheng-jie

（College of Horticulture and Forestry，Huazhong Agricultural University；National Center for Vegetable Improvement（Centeral China）；Key Laboratory of Horticulture Plant Biology，Ministry of Education，Wuhan 430070，China）

Received:2011-01-11 Revised:2011-05-03 Online:2011-06-25 Published:2011-06-25
Contact: XU Yue-jin

Abstract

Abstract: The change of chlorophyll content and the expression of pheophytin pheophorbide hydrolase gene（PPH）and pheidea oxygenase gene（PAO）were analyzed during leaf senescence of Cucumis sativus L. var. xishuangbannanesis Qi et Yuan and‘Huahuang 5’. The results showed that chlorophyll a and b content decreased significantly during the leaf senescence. The ratio of chlorophyll a and b was around 0.5，suggesting that chlorophyll b content might play a role in keeping green. Real-time PCR results showed that PPH and PAO had the highest expression in leaves of xishuangbannanesis Qi et Yuan at 35 d，with the relative expression values of 5.50 and 1.86，respectively. The maximum expression values of PPH in leaf of‘Huahuang 5’occurred at 35 d，with relative expression value of 7.14. The highest expression of PAO in leaf of‘Huahuang 5’occurred at 35 d and 45 d，with the relative expression values of 3.90 and 3.91，respectively. The expression of PPH and PAO in leaves of Cucumis sativus L. var. xishuangbannanesis Qi et Yuan were lower than that in‘Huahuang 5’.

Key words: cucumber, chlorophyll, breakdown, PPH, PAO, senescence, expression

CLC Number:

S 642.2

WANG Wei;XU Yue-jin;and WAN Zheng-jie . Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber [J]. ACTA HORTICULTURAE SINICA, 2011, 38(6): 1104-1110.

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Cloning and Expression Analysis of Key Genes PPH and PAO for Chlorophyll Degradation in Cucumber

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