Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

doi:10.16420/j.issn.0513-353x.2021-0807

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1907-1921.doi: 10.16420/j.issn.0513-353x.2021-0807

• Research Papers • Previous Articles Next Articles

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

TAN Shanshan¹, QIU Liang¹^,³, DUAN Aoqi¹, SHU Sheng¹^,², ZENG Xiaoping⁴, ZHU Weimin⁵, JIA Min¹, YAN Jun⁵, LIU Yanhua¹, LIU Hui¹, XIONG Aisheng¹()

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
²Suqian Academy of Protected Horticultures,Suqian,Jiangsu 223800,China
³Institute of Agricultural Sciences in Riverside Region of Jiangsu,Nantong,Jiangsu 226001,China
⁴Jiangsu Agricultural Technical Extension Station,Nanjing 210036,China
⁵Horticulture Research Institute,Shanghai Academy Agricultural Sciences,Shanghai 201403,China

Received:2022-01-04 Revised:2022-06-10 Online:2022-09-25 Published:2022-10-08

Abstract

Abstract:

Celery seeds are small and contain a large amount of germination-inhibiting substances,which brings difficulties to celery seeding propagation. The seeds of‘Liuhe Huangxinqin’and‘Saixue’were treated with different concentrations（0,5%,10%,20%,40%）of NaClO cultured in petri dish and acupoint dish. The seed germination index,seedling growth index and photosynthetic parameters were measured. In addition,the changes of chlorophyll content and related gene expression in seedlings were analyzed. The results showed that celery seeds treated with 5% NaClO had the highest germination rate,germination potential,germination index and vigor index,and the strongest seedling growth potential. And the seedling vigor index,chlorophyll content and net photosynthetic rate were also increased to some extent. Also,the relative expression levels of related genes were also higher. These results suggested that the appropriate concentration for NaClO to promote celery seeds is 5%.

Key words: celery, seed, sodium hypochlorite, germination, seedling, chlorophyll

CLC Number:

S636.3

TAN Shanshan, QIU Liang, DUAN Aoqi, SHU Sheng, ZENG Xiaoping, ZHU Weimin, JIA Min, YAN Jun, LIU Yanhua, LIU Hui, XIONG Aisheng. Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery[J]. Acta Horticulturae Sinica, 2022, 49(9): 1907-1921.

Figures/Tables 11

References 46

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基因Gene	序列（5′-3′）Sequence
脱植基叶绿素a氧化酶基因 CAO	F：TTGGTGAATGATAGGCTGTT;R：GGATGGTAAGGTTGGACTG
叶绿素b还原酶基因 NOL	F：TCTCTTCTTCGCATCATCTT;R：TTGAGCCAGCATTGTTGA
7-羟甲基叶绿素a还原酶基因 HCAR	F：CCAACTTAGCCTTATCATCTG;R：TGCCACAACATCCACAAT
H亚基镁螯合酶基因 ChlH	F：GAATAGTAGCCGCATAGGAT;R：ACAAGAAGGTAGCCATCAC
镁原卟啉氧甲基转移酶基因 ChlM	F：TATCAGTCTCCGCCATTTTCCCTCA;R：GTGTCCCCGTATATTTTCTTCCACC
镁原卟啉Ⅸ单酯环化酶基因 MPE	F：ATGATACACTGAAGCGGATT;R：CCACTAATAAGCAGCAACAA
脱镁叶绿素酶基因 PPH	F：CAAGCAGAGGCATCACAA;R：CCGTATAATGGAGACAACAAG
脱镁叶绿酸氧化酶基因 PAO	F：ATGTGGAGGAACGGAAGA;R：GTAGGCATCTAACGGAAGG
芹菜内参基因 Agactin	F：CTTCCTGCCATATATGATTGG;R：GCCAGCACCTCGATCTTCATG

基因Gene	序列（5′-3′）Sequence
脱植基叶绿素a氧化酶基因 CAO	F：TTGGTGAATGATAGGCTGTT;R：GGATGGTAAGGTTGGACTG
叶绿素b还原酶基因 NOL	F：TCTCTTCTTCGCATCATCTT;R：TTGAGCCAGCATTGTTGA
7-羟甲基叶绿素a还原酶基因 HCAR	F：CCAACTTAGCCTTATCATCTG;R：TGCCACAACATCCACAAT
H亚基镁螯合酶基因 ChlH	F：GAATAGTAGCCGCATAGGAT;R：ACAAGAAGGTAGCCATCAC
镁原卟啉氧甲基转移酶基因 ChlM	F：TATCAGTCTCCGCCATTTTCCCTCA;R：GTGTCCCCGTATATTTTCTTCCACC
镁原卟啉Ⅸ单酯环化酶基因 MPE	F：ATGATACACTGAAGCGGATT;R：CCACTAATAAGCAGCAACAA
脱镁叶绿素酶基因 PPH	F：CAAGCAGAGGCATCACAA;R：CCGTATAATGGAGACAACAAG
脱镁叶绿酸氧化酶基因 PAO	F：ATGTGGAGGAACGGAAGA;R：GTAGGCATCTAACGGAAGG
芹菜内参基因 Agactin	F：CTTCCTGCCATATATGATTGG;R：GCCAGCACCTCGATCTTCATG

品种 Cultivar	NaClO/%	发芽势/% Germination potential		发芽率/% Germination rate		发芽指数 Germination index		活力指数 Vigor index
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	65.33 a	56.00 ab	82.67 a	70.44 ab	6.82 a	10.08 ab	144.90 a	286.10 b
	5	77.33 a	69.33 a	83.33 a	81.78 a	7.06 a	11.89 a	173.51 a	371.48 a
	10	62.00 a	47.11 bc	76.00 ab	64.89 abc	6.38 ab	9.13 bc	149.86 a	224.99 bc
	20	62.00 a	49.11 b	73.33 ab	62.00 bc	6.09 ab	8.88 bc	156.52 a	211.95 c
	40	42.67 b	31.11 c	62.00 b	51.56 c	4.92 b	6.78 c	79.69 b	110.52 d
赛雪 Saixue	0	59.67 b	61.33 a	76.67 b	73.11 ab	6.14 b	10.60 ab	178.35 b	302.60 b
	5	75.87 a	64.03 a	91.33 a	78.33 a	7.54 a	11.26 a	235.33 a	396.21 a
	10	58.00 b	50.22 b	76.00 bc	68.44 ab	6.11 bc	9.68 bc	159.70 b	310.39 b
	20	61.33 b	49.33 b	72.67 bc	67.33 b	5.95 bc	9.56 bc	182.52 b	209.57 c
	40	47.00 c	41.22 c	62.00 c	64.67 b	4.96 c	8.95 c	79.97 c	223.81 c

品种 Cultivar	NaClO/%	发芽势/% Germination potential		发芽率/% Germination rate		发芽指数 Germination index		活力指数 Vigor index
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	65.33 a	56.00 ab	82.67 a	70.44 ab	6.82 a	10.08 ab	144.90 a	286.10 b
	5	77.33 a	69.33 a	83.33 a	81.78 a	7.06 a	11.89 a	173.51 a	371.48 a
	10	62.00 a	47.11 bc	76.00 ab	64.89 abc	6.38 ab	9.13 bc	149.86 a	224.99 bc
	20	62.00 a	49.11 b	73.33 ab	62.00 bc	6.09 ab	8.88 bc	156.52 a	211.95 c
	40	42.67 b	31.11 c	62.00 b	51.56 c	4.92 b	6.78 c	79.69 b	110.52 d
赛雪 Saixue	0	59.67 b	61.33 a	76.67 b	73.11 ab	6.14 b	10.60 ab	178.35 b	302.60 b
	5	75.87 a	64.03 a	91.33 a	78.33 a	7.54 a	11.26 a	235.33 a	396.21 a
	10	58.00 b	50.22 b	76.00 bc	68.44 ab	6.11 bc	9.68 bc	159.70 b	310.39 b
	20	61.33 b	49.33 b	72.67 bc	67.33 b	5.95 bc	9.56 bc	182.52 b	209.57 c
	40	47.00 c	41.22 c	62.00 c	64.67 b	4.96 c	8.95 c	79.97 c	223.81 c

品种 Cultivar	NaClO/%	根长/mm Root length		下胚轴长/mm Hypocotyl length		单株鲜质量/mg Fresh weight per plant		单株干质量/mg Dry weight per plant
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	21.25 ab	28.39 ab	9.59 bc	13.38 b	3.82 d	4.65 c	0.28 c	0.35 ab
	5	24.57 a	31.25 a	10.26 b	13.41 b	5.29 b	5.19 b	0.33 a	0.36 a
	10	23.50 a	24.64 b	14.59 a	10.61 b	5.43 a	5.27 a	0.33 a	0.36 a
	20	25.71 a	23.87 b	8.11 c	13.59 b	5.43 a	5.20 b	0.30 b	0.35 ab
	40	16.21 b	16.31 c	8.17 c	26.91 a	4.14 c	3.32 d	0.29 bc	0.22 c
赛雪Saixue	0	29.03 a	28.56 abc	14.47 a	10.35 b	4.20 d	5.99 c	0.30 c	0.46 b
	5	31.20 a	35.19 a	15.13 a	14.43 b	4.72 b	6.57 b	0.36 b	0.53 a
	10	26.15 ab	32.05 ab	11.51 b	11.07 b	4.80 a	6.99 a	0.47 a	0.42 bc
	20	30.69 a	21.93 c	11.41 b	13.89 b	4.62 c	5.65 d	0.36 b	0.37 d
	40	16.11 b	25.02 bc	8.73 c	20.01 a	4.61 c	6.53 b	0.30 c	0.38 cd

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

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苗龄/d Seeding age	品种 Cultivar	NaClO/%	株高/cm Height	茎粗/mm Diameter	鲜质量/mg Fresh weight		干质量/mg Dry weight		壮苗指数 Seedling strength index
苗龄/d Seeding age	品种 Cultivar	NaClO/%	株高/cm Height	茎粗/mm Diameter	地上部 Aboveground	地下部 Underground	地上部 Aboveground	地下部 Underground	壮苗指数 Seedling strength index
40	六合黄心芹 Liuhe Huangxinqin	0	12.10 a	0.78 bc	240 b	9.50 a	17.30 c	0.70 b	0.84 c
		5	12.77 a	0.98 a	300 a	9.10 b	21.50 a	0.87 a	1.08 a
		10	10.20 b	0.89 ab	290 a	7.40 c	19.10 b	0.73 b	0.93 b
		20	8.80 b	0.88 ab	180 c	4.10 e	12.10 d	0.47 c	0.61 d
		40	6.93 c	0.72 c	130 d	5.00 d	8.00 e	0.23 d	0.32 e
	赛雪Saixue	0	10.27 b	0.96 a	200 a	9.90 a	11.35 c	0.48 b	0.61 b
		5	12.20 a	0.98 a	210 a	9.90 a	12.50 a	0.83 a	0.99 a
		10	10.43 b	0.75 b	160 c	4.40 b	11.85 b	0.27 d	0.36 d
		20	10.37 b	0.74 b	150 c	4.98 b	9.80 d	0.33 c	0.41 c
		40	10.23 b	0.75 b	180 b	2.78 c	9.85 d	0.30 cd	0.38 cd
60	六合黄心芹 Liuhe Huangxinqin	0	13.20 b	1.03 c	540 b	32.10 b	36.20 b	3.30 bc	3.91 c
		5	16.80 a	1.68 a	580 a	89.60 a	40.00 a	7.70 a	9.66 a
		10	13.40 b	1.22 b	370 c	11.00 c	23.10 c	2.80 c	3.37 d
		20	11.20 c	0.95 cd	240 d	33.40 b	13.90 d	3.50 b	4.53 b
		40	10.40 cd	0.77 d	180 e	5.60 d	8.60 e	0.50 d	0.60 e
	赛雪Saixue	0	12.70 bc	1.02 bc	310 b	24.80 a	19.50 b	2.20 a	2.62 ab
		5	14.70 a	1.40 a	460 a	25.70 a	26.80 a	2.20 a	2.66 a
		10	11.90 d	1.09 b	290 c	22.40 b	17.30 b	1.60 c	1.92 d
		20	12.80 bc	0.95 c	210 d	21.10 b	12.90 c	2.10 ab	2.55 bc
		40	13.40 b	0.86 d	220 d	17.50 c	10.00 cd	1.70 b	2.06 cd

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