The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (5): 631-638.

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The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening

WEI Jian-mei^1,2 and MA Feng-wang^1*

(¹College of Horticulture, N orthwestA & F University, Yangling, Shaanx i 712100, China; ²Changli Fruit Institute, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China)

Received:2008-10-27 Revised:2009-01-22 Online:2009-05-25 Published:2009-05-25
Contact: MA Feng-wang

Abstract

Abstract: For understanding the role of β-galactosidase (β-Gal) and lipoxygenase (LOX) during fruit development, ripening and softening, the fruits of app le (Malus domestica Borkh. ) cultivars, ‘Fuji’and‘Golden Delicious’, were used to investigate the activity changes of β-Gal and LOX over this period, and
during postharvest regulation by ethylene. The results indicated that theβ2Gal activity in‘Golden Delicious’was markedly higher than that in‘Fuji’during fruit development and ripening and the difference was in particular significant during ripening and softening. The LOX activity in‘Fuji’was dramatically increased and higher than that in‘Golden Delicious’after flowering, but its activity wasmarkedly reduced and maintained lower than that of‘Golden Delicious’before fruit ripening. The β-Gal and LOX activities of app le fruitwere
markedly inhibited by 12methycyclopene ( 1-MCP) and increased by ethephon. The changes of β-Gal and LOX activity showed a similar trend and the β-Gal be much easier to be regulated by ethylene than LOX.These results suggested that the change of β-Gal and LOX activities be correlated with the storage characteristic of apple fruit and β-Galmight have an important role in the early stage of fruit softening.

Key words: apple, postharvest, β-Gal, LOX, activity, ethylene regulation

CLC Number:

S 661.1

WEI Jian-mei;and MA Feng-wang. The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening[J]. ACTA HORTICULTURAE SINICA, 2009, 36(5): 631-638.

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The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening

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