Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 432-440.doi: 10.16420/j.issn.0513-353x.2021-1118

• Research Notes • Previous Articles     Next Articles

Studies on the Antifungal Effect of Matrine on Colletotrichum Species on Tea Plant

CHENG Qinghua, ZHANG Zhipeng, WU Yanping, WAN Yuhe, CHEN Yingjuan*()   

  1. National Demonstration Center for Experimental Food Science and Technology Education,Department of Tea Science,College of Food Science,Southwest University,Chongqing 400715,China
  • Received:2022-09-17 Revised:2022-12-02 Online:2023-02-25 Published:2023-03-06
  • Contact: *(


In order to explore the application prospect of matrine in the prevention and treatment of tea disease,the antifungal activity of matrine on five different Colletotrichum species including C. fioriniaeC. gloeosporioidesC. chongqingenseC. karstii and C. camelliae has been determined in this study. The results showed that matrine had significant inhibitory activity against five different Colletotrichum species. EC50(concentration for 50% of maximal effect)of the five Colletotrichum species were 5.856,6.557,3.038,7.963 and 4.397 mg · mL-1,and the antifungal activity of different species are significantly different. At low concentrations(1-8 mg · mL-1),matrine has a better control effect on both C. chongqingense and C. camelliae. With the increase of the concentration,the inhibitory rates of the five Colletotrichum species were all above 95% at 22 mg · mL-1. Microscopic examination showed that matrine had a significant effect on the mycelial and conidial growth of five Colletotrichum species. With the increase of matrine concentration,part of hyphae expanded irregularly,the number of conidia decreased and the conidia grew abnormally. Results of the biological activity of Colletotrichum species showed that the cell structure were destroyed,the cell permeability increased,and the cell oxidative stress response enhanced. The results of this study confirmed the antifungal activity of matrine against Colletotrichum species,and preliminarily revealed its antimicrobial mechanism by damaging cell membrane,which provided a theoretical basis for the prevention and control of tea brown blight disease caused by Colletotrichum.

Key words: tea plant, matrine, Colletotrichum, antifungal activity, mechanism, conidium, bioactivity

CLC Number: