Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (12): 1733-1740.

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Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion

LIU Hao^{1, 2} ; L IU Yan-zhen^{1, 2 ;} WANG J ing^{1, 2} ; ZHENG Shao-quan³ , JIANG J i-mou³ ; CHEN Wei^{1, 2*}

(¹College of L ife Sciences, Fujian Agriculture and ForestUniversity, Fuzhou 350002, China; ² Proteom ics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ³ Fruit Research Institute, Fujian Academy of Agriculture Sciences,Fuzhou 350013, China)

Received:2009-08-23 Revised:2009-11-26 Online:2009-12-25 Published:2009-12-25

Abstract

Abstract: By comparing with two protein extraction methods, ensured the optimal method for longan seed proteins extraction. Proteomic approaches were used to analyze the changes of differentially expressed proteins during four different stages of longan seed abortion. Twenty-one differentially expressed proteins were detected on 2-DE gels, 9 were up-regulated and 12 were down-regulated. Among these proteins, 12 proteins were identified by MALDI/TOF /TOF-MS/MS and protein database searching. They were cytosolic malate dehydrogenase, Dihydrolipoamide adetyltransferase, 2, 3-bisphosphoglycerate-independent phosphoglycerate mutase, RuB isCO large subunit2binding p rotein subunit beta chlorop last p recursor, RuBisCO large subunit-binding protein subunit beta chloroplast precursor, 17 kD heat shock p rotein, ascorbate peroxidase, cytosolic ascorbate peroxidase, cysteine protease, dessication-related protein, and two unknown protein. These proteins were involved in energy and metabolism, chaperon, antioxidation, apoptosis, which might relate to seed abortion of longan.

Key words: seed, abortion, differentially expressed protein, two-dimensional electrophoresis (2-DE), mass spectrometry analysis

LIU Hao;;L IU Yan-zhen;;WANG J ing;;ZHENG Shao-quan;JIANG J i-mou;CHEN Wei;. Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1733-1740.

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Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion

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