e eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzlingin Papaya

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1427-1442.

• Fruit Trees • Previous Articles Next Articles

e eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzlingin Papaya

YAN Pu; SHEN Wen-tao.GAO Xin-zheng;LI Ya-li;ZHOU Peng^*

(Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture,institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences, Haikou571101, China)

Received:2009-05-25 Revised:2009-08-24 Online:2009-10-25 Published:2009-10-25
Contact: ZHOU Peng

Abstract

Abstract: eIF4E ( eukaryotic translation initiation factor 4E) family genes play important roles against virus from infecting plants. A complete cDNA of 995 bp from papaya was cloned using the method of RT-PCR and RACE. It was a isoform eIF4E, so itwas named CP-eIF ( iso) 4E (GenBank accession No. FJ595992).
The predicted CP-eIF ( iso) 4E protein had 206 amino acids, in which the active site was high conservative in primary structure, and the predicted virus-resistance site was located on the surface of cap-binding pocket in3-D structure. Semi-quantitative RT-PCR analysis indicated that CP-eIF ( iso) 4E expression was lower in leaf and higher in petal of papaya.

Key words: papaya, eIF ( iso) 4E, structure analysis, gene expression

CLC Number:

S 667.9
Q 78

YAN Pu;SHEN Wen-tao.GAO Xin-zheng;LI Ya-li;ZHOU Peng. e eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzlingin Papaya[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1427-1442.

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e eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzlingin Papaya

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