Cloning and Prokaryotic Expression of 14-3-3 Gene in Longan

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1431-1436.

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Cloning and Prokaryotic Expression of 14-3-3 Gene in Longan

YOU Xiang-rong¹;WANG Ping²;LIANGWen-yu¹;ZHENG Shao-quan³;CHEN Wei^1*

(¹College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ²College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ³ Fruit Research Institute, Fujian Academy of Agriculture Sciences,Fuzhou 350013, China)

Received:2009-05-21 Revised:2009-09-16 Online:2009-10-25 Published:2009-10-25
Contact: CHEN Wei

Abstract

Abstract: Full length cDNA of 14-3-3 gene, encoding a plant regulatory factor, was isolated from longan (Dimocarpus longan Lour. ) using homology based cloning and RACE methods. The accession number of the gene in GenBanks is FJ479618 (GI: 218202931). The full length cDNA is of 1 121 bp, with a 783 bp open reading frame encoding 261 amino acids. Homology analysis showed that the longan 14-3-3 cDNA has high consensus regions. Semi-quantitative RT-PCR analysis detected the exp ression of 14-3-3 gene in longan foliar
buds, leaves, mature flowers, and most up2regulated in flower buds. The p rokaryotic exp ression vector was-constructed and was expressed in E.coli; and a recombinant protein with a molecularweight of 34 kD was obtained by SDS-PAGE analysis. Western blotting confirmed the p rotein was 14-3-3 protein.

Key words: longan, flower bud, 14-3-3 gene, clone, prokaryotic expression

CLC Number:

S 667.2
Q 78

YOU Xiang-rong;WANG Ping;LIANGWen-yu;ZHENG Shao-quan;CHEN Wei. Cloning and Prokaryotic Expression of 14-3-3 Gene in Longan[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1431-1436.

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Cloning and Prokaryotic Expression of 14-3-3 Gene in Longan

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