Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 849-859.doi: 10.16420/j.issn.0513-353x.2020-0895

• Research Papers •     Next Articles

Identification and Expression Analysis of HSP90 Gene Family Under High Temperature Stress in Apple

YAO Fuwen1,*, WANG Meige1,*, SONG Chunhui1, SONG Shangwei1, JIAO Jian1, WANG Miaomiao1, WANG Kun2, BAI Tuanhui1,**(), ZHENG Xianbo1,**()   

  1. 1College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China
    2Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Received:2020-12-29 Revised:2021-04-08 Online:2021-05-25 Published:2021-06-07
  • Contact: BAI Tuanhui,ZHENG Xianbo;


In order to explore the role of heat shock protein(HSP90)gene family members in heat tolerance of apple(Malus × domestica Borkh.),bioinformatics method was used to identify the apple HSP90 gene family members,and their physicochemical properties,gene structure,cis elements,phylogenetic relationship and expression characteristics of HSP90 gene family under high temperature stress as well as in different tissues were analyzed. The results showed that a total of 11 HSP90 family members were identified in apple genome,which were distributed on 10 chromosomes. The amino acid sequence size was ranged from 104 to 818 aa,and molecular weight of protein ranged from 11.81 to 93.58 kD. Transcriptome analysis showed that four genes(MdHSP90-1,MdHSP90-3,MdHSP90-5and MdHSP90-11)of the 11 members were significantly up-regulated under high temperature stress. Subcellular localization analysis showed that MdHSP90-1,MdHSP90-3 and MdHSP90-5 proteins were located in the cell cytoplasm and nucleus,whereas MdHSP90-11 was targeted to the nucleus. Subsequent qRT-PCR analysis showed that the expression trend of the four genes in the two kinds of apple rootstocks were similar,the expression level increased greatly in the early stage of high temperature stress,and decreased in the middle and late stage. Four genes were also detected in different apple tissues,and the expressions of fourHSP90 genes were induced by heat stress to varying degrees,which indicated that the above four HSP90 genes may play an important role in the heat tolerance of apple.

Key words: apple, HSP90, high temperature stress, gene, structure, gene expression

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