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ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (1): 57-58.

• 研究报告 • Previous Articles     Next Articles

Transformation of Apple Using Super Expression Cowpea Trypsin Inhibitor(CpTI) Gene

Da Kedong1, Cui Decai1, Zhang Song1, Jing Demin2, Wang Bin2, and Shu Huairui1   

  1. (1Department of Horticulture, Shandong Agricultural University , Tai'an 271018; 2 Institute of Genetics , Acadmia Sinica, Beijing 100101)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

Abstract: Cowpea trypsin inhibitor ( CpTI) gene in super expression binary vector PECp was transferred to apple cultivar ‘Gala’ using Agrobacterium mediated leaf disc transformation method. GUS staining and southern blot confirmed that CpTI gene was inserted into apple genome.

Key words: Apple, CpTI gene, Super Promoter, Transformation

CLC Number: