Genome-Wide Identification of ABA Receptor Gene Family in Citrus and Functional Analysis of CrPYL9 in Fruit Maturation

doi:10.16420/j.issn.0513-353x.2025-0791

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (6): 1590-1612.doi: 10.16420/j.issn.0513-353x.2025-0791

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Genome-Wide Identification of ABA Receptor Gene Family in Citrus and Functional Analysis of CrPYL9 in Fruit Maturation

LIU Shumei¹^,², LÜ Yuanda¹, JIANG Bo¹, LIU Taibo²^,^*(), YAN Huaxue¹^,^*()

¹ Institute of Fruit Tree Research， Guangdong Academy of Agricultural Sciences，Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization，Ministry of Agriculture and Rural Affairs，Guangdong Provincial Key Laboratory of Fruit Tree Science and Technology， Guangzhou 510640， China
² Guangdong Laboratory for Lingnan Modern Agriculture， Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops，College of Life Sciences，South China Agricultural University，State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources，Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms， Guangzhou 510642， China

Received:2025-11-07 Revised:2025-12-21 Online:2026-06-24 Published:2026-06-24
Contact: LIU Taibo, YAN Huaxue

Abstract

Abstract:

The abscisic acid（ABA）receptor Pyrabactin resistance 1/PYR1-Like/Regulatory component of ABA receptor（PYR/PYL/RCAR，PYL）is an essential component of the ABA signaling pathway，responsible for regulating various physiological processes in plants such as growth，development，and stress responses. In this study，a total of 11 and 14 PYL genes were identified in sweet orange[Citrus sinensis（L.）Osbeck]and Clementine mandarin（C. clementina Hort. ex Tan.），respectively. Through phylogenetic analysis，citrus PYL genes were categorized into three distinct subfamilies. The expression patterns of citrus PYL genes showed tissue-specific characteristics and diverse responses to external hormone treatments. The results of molecular genetic experiments further confirmed that CrPYL9 positively regulates fruit maturation and color change，providing new insights into the mechanism of ABA-mediated regulation of non-climacteric fruit maturation.

Key words: Citrus, abscisic acid receptor, gene family, fruit maturation

LIU Shumei, LÜ Yuanda, JIANG Bo, LIU Taibo, YAN Huaxue. Genome-Wide Identification of ABA Receptor Gene Family in Citrus and Functional Analysis of CrPYL9 in Fruit Maturation[J]. Acta Horticulturae Sinica, 2026, 53(6): 1590-1612.

Figures/Tables 10

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基因 Gene	引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
CrPYL1	PYL1qF	TTGGAGATGCTGGATGATGAG	PYL1qR	AACCGATGTGACTGAACGATAA
CrPYL2	PYL1qF	CTGGTCTGGTTCGCTACT	PYL1qR	TCGCCACATACGACTTAA
CrPYL3	PYL1qF	GGTTGGTATTGGCTGATT	PYL1qR	AATAGCATCCTCCATTGTT
CrPYL4	PYL1qF	CCTAATAACACAACGGATT	PYL1qR	TTCTCTTATGCTGCCTAC
CrPYL5	PYL1qF	ATGGCTGATAATGATGAT	PYL1qR	ATGTTGTT AGAGTTGGAA
CrPYL6	PYL1qF	ATGAACGGTGTTGATGCG	PYL1qR	GGCTTGTACTTCTGTGGC
CrPYL7-1	PYL1qF	TGGCAAGATTGAGGAGGA	PYL1qR	AGTTGTAGCGGGAAGACC
CrPYL7-2	PYL1qF	TCAACCGCAGAAGTATAAGC	PYL1qR	GACCGTAACAATAGACGAGTAG
CrPYL7-3	PYL1qF	GGCAAGATTGAGGAGGAC	PYL1qR	AGTTGTAGCGGGAAGACC
CrPYL8	PYL1qF	CGGTGGTCGTGGAGTCTTA	PYL1qR	CTGCTGATGCTGCTGTTGT
CrPYL9	PYL1qF	AACCACAACCCACAACCCTAAGT	PYL1qR	CGCCTGACCACGGACCAA
CrPYL10	PYL1qF	CTGTTCCGTCTCCGATAA	PYL1qR	CGTCCAGAATGTCAAGTC
CrPYL11	PYL1qF	ATGGAGGAGCTGAAAGTG	PYL1qR	ACATCTGCTAACGAAAGG
CrActin	ActinqF	AGAACTATGAACTGCCTGATGGC	ActinqR	GCTTGGAGCAAGTGCTGTGATT
CrPYL9	CDS F	AATCGACTCTAGAAAGCTTATGCCTGCCTCATCAATACAAG	CDS R	CTAGTATTTAAATGTCGACTTACTTGTTCGATCTAGCAGCCATG
CrPYL9	TRV-PYL9F	AAGGTTACCGAATTCTCTAGACGTTTGGTCCGTGGTCAG	TRV-PYL9R	GAGACGCGTGAGCTCGGTACCGCATGGCGAAGCGTGTAG

基因 Gene	引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
CrPYL1	PYL1qF	TTGGAGATGCTGGATGATGAG	PYL1qR	AACCGATGTGACTGAACGATAA
CrPYL2	PYL1qF	CTGGTCTGGTTCGCTACT	PYL1qR	TCGCCACATACGACTTAA
CrPYL3	PYL1qF	GGTTGGTATTGGCTGATT	PYL1qR	AATAGCATCCTCCATTGTT
CrPYL4	PYL1qF	CCTAATAACACAACGGATT	PYL1qR	TTCTCTTATGCTGCCTAC
CrPYL5	PYL1qF	ATGGCTGATAATGATGAT	PYL1qR	ATGTTGTT AGAGTTGGAA
CrPYL6	PYL1qF	ATGAACGGTGTTGATGCG	PYL1qR	GGCTTGTACTTCTGTGGC
CrPYL7-1	PYL1qF	TGGCAAGATTGAGGAGGA	PYL1qR	AGTTGTAGCGGGAAGACC
CrPYL7-2	PYL1qF	TCAACCGCAGAAGTATAAGC	PYL1qR	GACCGTAACAATAGACGAGTAG
CrPYL7-3	PYL1qF	GGCAAGATTGAGGAGGAC	PYL1qR	AGTTGTAGCGGGAAGACC
CrPYL8	PYL1qF	CGGTGGTCGTGGAGTCTTA	PYL1qR	CTGCTGATGCTGCTGTTGT
CrPYL9	PYL1qF	AACCACAACCCACAACCCTAAGT	PYL1qR	CGCCTGACCACGGACCAA
CrPYL10	PYL1qF	CTGTTCCGTCTCCGATAA	PYL1qR	CGTCCAGAATGTCAAGTC
CrPYL11	PYL1qF	ATGGAGGAGCTGAAAGTG	PYL1qR	ACATCTGCTAACGAAAGG
CrActin	ActinqF	AGAACTATGAACTGCCTGATGGC	ActinqR	GCTTGGAGCAAGTGCTGTGATT
CrPYL9	CDS F	AATCGACTCTAGAAAGCTTATGCCTGCCTCATCAATACAAG	CDS R	CTAGTATTTAAATGTCGACTTACTTGTTCGATCTAGCAGCCATG
CrPYL9	TRV-PYL9F	AAGGTTACCGAATTCTCTAGACGTTTGGTCCGTGGTCAG	TRV-PYL9R	GAGACGCGTGAGCTCGGTACCGCATGGCGAAGCGTGTAG

蛋白 Protein	序列ID Sequence ID	氨基酸数量 Number of amino acids	分子量/Da Molecular weight	等电点 pI	不稳定系数 Instability index	脂肪族aa 系数 Aliphatic index	亲水性指数 Hydropathy index	亚细胞定位 Subcellular localization
CsPYL1	Cs_ont_1g020400.1	216	23 982.21	4.91	32.15	87.04	-0.167	Cyt
CrPYL1	Ciclev10027552m.1	187	20 974.68	4.90	30.81	80.70	-0.327	Chl，Nu
CsPYL2	Cs_ont_4g004630.1	172	19 641.45	5.39	37.01	77.67	-0.289	Cyt
CrPYL2	Ciclev10009701m.1	172	19 627.42	5.39	36.51	77.09	-0.287	Cyt，Chl，Nu
CsPYL3	Cs_ont_4g004660.1	179	19 936.37	5.11	31.66	76.26	-0.322	Chl，Cyt，Nu
CrPYL3	Ciclev10010269m.1	179	19 969.45	5.46	31.22	77.37	-0.292	Cyt，Nu
CsPYL4	Cs_ont_4g005330.1	190	21 382.28	5.90	43.51	85.00	-0.343	Cyt
CrPYL4	Ciclev10010262m.1	190	21 382.28	5.90	43.51	85.00	-0.343	Cyt
CsPYL5	Cs_ont_4g008950.1	181	20 580.22	4.74	37.32	70.55	-0.42	Chl，Cyt，Nu
CrPYL5	Ciclev10009650m.1	181	20 589.23	4.82	43.74	70.55	-0.418	Cyt，Chl，Nu
CsPYL6	Cs_ont_5g004380.1	186	21 089.04	6.44	41.65	91.56	-0.351	Chl
CrPYL6	Ciclev10022440m.1	186	21 089.04	6.44	41.65	91.56	-0.351	Chl
CsPYL7	Cs_ont_5g049350.1	197	22 204.13	5.85	39.57	89.85	-0.374	Chl
CrPYL7-1	Ciclev10022578m.1	168	19 176.97	8.88	51.75	84.58	-0.421	Chl，Nu
CrPYL7-2	Ciclev10022206m.1	214	24 098.33	6.11	38.98	91.82	-0.293	Chl
CrPYL7-3	Ciclev10022353m.1	197	22 144.12	5.98	39.63	91.83	-0.332	Chl，Nu
CsPYL8	Cs_ont_6g013980.1	245	26 654.90	7.69	44.67	84.61	-0.284	Cyt
CrPYL8	Ciclev10012583m.1	245	26 654.90	7.69	44.67	84.61	-0.284	Cyt
CsPYL9	Cs_ont_7g002120.1	201	22 137.99	7.68	62.22	77.01	-0.344	Cyt
CrPYL9	Ciclev10032781m.1	201	22 137.99	7.68	62.22	77.01	-0.344	Cyt
CsPYL10	Cs_ont_7g010910.1	165	18 735.69	5.51	40.06	69.70	-0.656	Nu
CrPYL10	Ciclev10012769m.1	209	23 268.87	5.08	35.06	82.44	-0.362	Cyt，Nu
CsPYL11	Cs_ont_9g027270.1	189	21 335.19	6.41	43.50	96.88	-0.413	Nu
CrPYL11	Ciclev10005981m.1	189	21 335.19	6.41	43.50	96.88	-0.413	Nu
CrPYL12	Ciclev10033656m.1	158	17 571.81	4.70	35.71	80.76	-0.282	Chl

蛋白 Protein	序列ID Sequence ID	氨基酸数量 Number of amino acids	分子量/Da Molecular weight	等电点 pI	不稳定系数 Instability index	脂肪族aa 系数 Aliphatic index	亲水性指数 Hydropathy index	亚细胞定位 Subcellular localization
CsPYL1	Cs_ont_1g020400.1	216	23 982.21	4.91	32.15	87.04	-0.167	Cyt
CrPYL1	Ciclev10027552m.1	187	20 974.68	4.90	30.81	80.70	-0.327	Chl，Nu
CsPYL2	Cs_ont_4g004630.1	172	19 641.45	5.39	37.01	77.67	-0.289	Cyt
CrPYL2	Ciclev10009701m.1	172	19 627.42	5.39	36.51	77.09	-0.287	Cyt，Chl，Nu
CsPYL3	Cs_ont_4g004660.1	179	19 936.37	5.11	31.66	76.26	-0.322	Chl，Cyt，Nu
CrPYL3	Ciclev10010269m.1	179	19 969.45	5.46	31.22	77.37	-0.292	Cyt，Nu
CsPYL4	Cs_ont_4g005330.1	190	21 382.28	5.90	43.51	85.00	-0.343	Cyt
CrPYL4	Ciclev10010262m.1	190	21 382.28	5.90	43.51	85.00	-0.343	Cyt
CsPYL5	Cs_ont_4g008950.1	181	20 580.22	4.74	37.32	70.55	-0.42	Chl，Cyt，Nu
CrPYL5	Ciclev10009650m.1	181	20 589.23	4.82	43.74	70.55	-0.418	Cyt，Chl，Nu
CsPYL6	Cs_ont_5g004380.1	186	21 089.04	6.44	41.65	91.56	-0.351	Chl
CrPYL6	Ciclev10022440m.1	186	21 089.04	6.44	41.65	91.56	-0.351	Chl
CsPYL7	Cs_ont_5g049350.1	197	22 204.13	5.85	39.57	89.85	-0.374	Chl
CrPYL7-1	Ciclev10022578m.1	168	19 176.97	8.88	51.75	84.58	-0.421	Chl，Nu
CrPYL7-2	Ciclev10022206m.1	214	24 098.33	6.11	38.98	91.82	-0.293	Chl
CrPYL7-3	Ciclev10022353m.1	197	22 144.12	5.98	39.63	91.83	-0.332	Chl，Nu
CsPYL8	Cs_ont_6g013980.1	245	26 654.90	7.69	44.67	84.61	-0.284	Cyt
CrPYL8	Ciclev10012583m.1	245	26 654.90	7.69	44.67	84.61	-0.284	Cyt
CsPYL9	Cs_ont_7g002120.1	201	22 137.99	7.68	62.22	77.01	-0.344	Cyt
CrPYL9	Ciclev10032781m.1	201	22 137.99	7.68	62.22	77.01	-0.344	Cyt
CsPYL10	Cs_ont_7g010910.1	165	18 735.69	5.51	40.06	69.70	-0.656	Nu
CrPYL10	Ciclev10012769m.1	209	23 268.87	5.08	35.06	82.44	-0.362	Cyt，Nu
CsPYL11	Cs_ont_9g027270.1	189	21 335.19	6.41	43.50	96.88	-0.413	Nu
CrPYL11	Ciclev10005981m.1	189	21 335.19	6.41	43.50	96.88	-0.413	Nu
CrPYL12	Ciclev10033656m.1	158	17 571.81	4.70	35.71	80.76	-0.282	Chl

Genome-Wide Identification of ABA Receptor Gene Family in Citrus and Functional Analysis of CrPYL9 in Fruit Maturation

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