Identification of the TCP Gene Family in Brassica campestris and Analysis of Their Effects on Axillary Bud Traits

doi:10.16420/j.issn.0513-353x.2025-0094

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (1): 82-98.doi: 10.16420/j.issn.0513-353x.2025-0094

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification of the TCP Gene Family in Brassica campestris and Analysis of Their Effects on Axillary Bud Traits

ZHU Caishuo¹^,², LI Jianzhong²^,³, ZHANG Qiannan²^,⁴, XU Lijie²^,⁵, ZHANG Shujiang², ZHANG Shifan², LI Fei², ZHANG Hui², XU Jin¹, SONG Zihan²^,^**(), LI Guoliang²^,^**()

¹ College of Horticulture， Shanxi Agricultural University，Taigu， Shanxi 030801， China
² State Key Laboratory of Vegetable Biobreeding， Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences， Beijing 100081， China
³ College of Horticulture， Anhui Agricultural University， Hefei 230036， China
⁴ Guyuan Branch， Ningxia Academy of Agricultural and Forestry Sciences，Guyuan， Ningxia 756000， China
⁵ College of Forestry， Shenyang Agricultural University， Shenyang 110866， China

Received:2025-04-17 Revised:2025-09-19 Online:2026-01-25 Published:2026-01-26
Contact: SONG Zihan, LI Guoliang

Abstract

Abstract:

Elucidating the mechanism by with the TCP gene family regulates of axillary buds in Brassica campestris is important for enhancing the plant architecture of B. campestris. Based on the homology between Arabidopsis TCP family members and the Brassica rapa genome，the TCP family members of Brassica campestris were screened and identified for physicochemical properties， phylogenetic relationships，gene structure，chromosomal localization，and covariance analysis，and the expression of TCP family genes in different Brassica campestris materials was also analyzed，and key genes related to the development of axillary buds of Brassica campestris were further identified for subcellular localization analysis. The results showed that a total of 39 TCP family members were identified in Brassica campestris，all of which contained conserved TCP structural domains. the TCP family genes were distributed unevenly on the chromosome. By selecting four kinds of Brassica campestris materials with different numbers of axillary buds for real-time fluorescence quantitative analysis，it was found that the TCP family of BraA05g005380.3C，BraA07g034590.3C，BraA03g048240.3C，BraA02g018710.3C，BraA03g038400.3C，and BraA01g034360.3C had significant expression differences，and it was hypothesized that these genes were key candidate genes for positively regulating axillary bud growth in Brassica campestris. Subcellular localization analysis of the above six genes revealed that the BraA07g034590.3C gene was localized to the nuclear membrane of the cell，while the rest of the genes were localized in the nucleus.

Key words: Brassica campestris, TCP, gene family, axillary bud, regulation gene

ZHU Caishuo, LI Jianzhong, ZHANG Qiannan, XU Lijie, ZHANG Shujiang, ZHANG Shifan, LI Fei, ZHANG Hui, XU Jin, SONG Zihan, LI Guoliang. Identification of the TCP Gene Family in Brassica campestris and Analysis of Their Effects on Axillary Bud Traits[J]. Acta Horticulturae Sinica, 2026, 53(1): 82-98.

Figures/Tables 10

References 50

[1]	Aguilar-Martínez J A, Poza-Carrión C, Cubas P. 2007. Arabidopsis BRANCHED 1 acts as an integrator of branching signals within axillary buds. The Plant Cell, 19 (2):458-472. doi: 10.1105/tpc.106.048934 URL
[2]	Braun N, de Saint Germain A, Pillot J P, Boutet-Mercey S, Dalmais M, Antoniadi I, Li X, Maia-Grondard A, Le Signor C, Bouteiller N, Luo D, Bendahmane A, Turnbull C, Rameau C. 2012. The pea TCP transcription factor PsBRC 1 acts downstream of Strigolactones to control shoot branching. Plant Physiology, 158 (1):225-238. doi: 10.1104/pp.111.182725 pmid: 22045922
[3]	Chai W, Jiang P, Huang G, Jiang H, Li X. 2017. Identification and expression profiling analysis of TCP family genes involved in growth and development in maize. Physiology and Molecular Biology of Plants, 23 (4):779-791. doi: 10.1007/s12298-017-0476-1 pmid: 29158628
[4]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools:an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202. doi: 10.1016/j.molp.2020.06.009 URL
[5]	Chen L, Chen Y Q, Ding A M, Chen H, Xia F, Wang W F, Sun Y H. 2016. Genome-wide analysis of TCP family in tobacco. Genet Mol Res, 15 (2):10.
[6]	Chen Zhihua, Qiao Zhensheng, Li Jiaqi, Zhang Xiaolin, Ma Shaojie, He Chengzhong, Zong Dan. 2024. Genome-wide identification and analysis of the TCP gene family in Populus yunnanensis. Biotechnology Bulletin, 40 (11):214-226. (in Chinese) doi: 10.13560/j.cnki.biotech.bull.1985.2024-0115
	陈智华, 乔振升, 李嘉其, 张晓琳, 马少杰, 何承忠, 纵丹. 2024. 滇杨TCP基因家族的全基因组鉴定与分析. 生物技术通报, 40 (11):214-226. doi: 10.13560/j.cnki.biotech.bull.1985.2024-0115
[7]	Danisman S, van Dijk A D, Bimbo A, van der Wal F, Hennig L, de Folter S. 2013. Analysis of functional redundancies within the Arabidopsis TCP transcription factor family. Journal of Experimental Botany,64:5673-5685.
[8]	Dong Xuanke, Li Yingyang, Ma Yuwan, Cai Yanfang, Ding Qihan, Chen Haixia, Li Yufan, Chen Jiren. 2025. The functional analysis of RcTCP9 gene in response to salt and drought stress in Rosa chinensis‘Yueyuehong’. Acta Horticulturae Sinica, 52 (7):1789-1802. (in Chinese) doi: 10.16420/j.issn.0513-353x.2024-0791
	董炫克, 李映旸, 马豫皖, 蔡艳芳, 丁启涵, 陈海霞, 李玉帆, 陈己任. 2025. 月季‘月月红’RcTCP9基因在盐和干旱胁迫中的功能分析. 园艺学报, 52 (7):1789-1802. doi: 10.16420/j.issn.0513-353x.2024-0791
[9]	Du Shanshan, Zhou Shaoli, Zhao Baolin, Zhao Weiyue, Chen Jianghua. 2022. Identification and analysis of TCP gene family in Pisum sativum. Plant Physiology Journal, 58 (8):1575-1586. (in Chinese)
	杜姗姗, 周绍礼, 赵宝林, 赵维月, 陈江华. 2022. 豌豆TCP基因家族的鉴定与分析. 植物生理学报, 58 (8):1575-1586.
[10]	Duan Longfei, Li Wenyan, Mu Xiaoqian, Zhang Yuanyuan, Guo Bangli, Chen Guoai. 2016. Molecular evolution and gene expression of Jasmonic acid receptor COI protein family. Chinese Journal of Bioinformatics, 14 (3):146-155. (in Chinese)
	段龙飞, 李文燕, 慕小倩, 张园园, 郭邦利, 陈国爱. 2016. 茉莉酸信号受体COI蛋白家族的分子进化与基因表达分析. 生物信息学, 14 (3):146-155.
[11]	Feng Xianjun, Wu Yinghua, Shi Yan, Hou Leiping, Li Meilan. 2022. Identification and expression analysis of acid sucrose invertase gene family in Chinese flowering cabbage. Jiangsu Agricultural Sciences, 50 (19):44-50. (in Chinese)
	冯献君, 吴英华, 史艳, 侯雷平, 李梅兰. 2022. 菜心酸性蔗糖转化酶基因家族的鉴定及表达分析. 江苏农业科学, 50 (19):44-50.
[12]	Finlayson S A. 2007. Arabidopsis teosinte branched1-like 1 regulates axillary bud outgrowth and is homologous to monocot teosinte branched1. Plant & Cell Physiology, 48 (5):667-677.
[13]	González-Grandío E, Pajoro A, Franco-Zorrilla J M, Tarancón C, Immink R G, Cubas P. 2017. Abscisic acid signaling is controlled by a BRANCHED1/HD-ZIP I cascade in Arabidopsis axillary buds. Proceedings of the National Academy of Sciences of the United States of America, 114 (2):E245-E254.
[14]	Huang F, Shi C, Zhang Y, Hou X. 2022. Genome-wide identification and characterization of TCP family genes in pak-choi[Brassica campestris(syn. Brassica rapa)ssp. chinensis var. communis]. Frontiers in Plant Science,13:854171.
[15]	Hou Xilin, Li Ying, Huang Feiyi. 2020. New advances in molecular biology of main characters and breeding technology in non heading Chinese cabbage(Brassica campestris ssp. chinensis). Acta Horticulturae Sinica, 47 (9):1663-1677. (in Chinese)
	侯喜林, 李英, 黄菲艺. 2020. 不结球白菜(Brassica campestris ssp. chinensis)主要性状及育种技术的分子生物学研究新进展. 园艺学报, 47 (9):1663-1677.
[16]	Huang Yilin, Xu Yufu, Li Ronghua, Li Guangguang, Zhang Hua, Guo Peiguo, Xia Yanshi. 2023. Genetic variations of AUF2 gene and their association with agronomic traits in flowering Chinese cabbage. Guangdong Agricultural Sciences, 50 (4):33-41. (in Chinese)
	黄依琳, 许玉富, 李荣华, 李光光, 张华, 郭培国, 夏岩石. 2023. 菜心群体中AUF2基因的遗传变异及其与农艺性状的关联分析. 广东农业科学, 50 (4):33-41.
[17]	Koyama T, Furutani M, Tasaka M, Ohme-Takagi M. 2007. TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis. The Plant Cell, 19 (2):473-484. doi: 10.1105/tpc.106.044792 URL
[18]	Li Kunjie, Tan Shanshan, Sun Bo, Rao Dan, He Qi, Min Ailing, Li Mengyao. 2019. Genome-wide identification and analysis of TCP transcription factor family in Brassica juncea. Journal of Sichuan Agricultural University, 37 (4):459-468. (in Chinese)
	李坤杰, 谭杉杉, 孙勃, 饶丹, 何琦, 闵爱玲, 李梦瑶. 2019. 芥菜TCP转录因子家族全基因组鉴定及表达分析. 四川农业大学学报, 37 (4):459-468.
[19]	Li Z Y, Li B, Dong A W. 2012. The Arabidopsis transcription factor AtTCP15 regulates endoreduplication by modulating expression of key cell-cycle genes. Mol Plant, 5 (1):270-280. doi: 10.1093/mp/ssr086 URL
[20]	Liu D H, Luo Y, Han H, Liu Y Z, Alam S M, Zhao H X, Li Y T. 2022. Genome-wide analysis of citrus TCP transcription factors and their responses to abiotic stresses. BMC Plant Biology, 22 (1):325. doi: 10.1186/s12870-022-03709-3
[21]	Liu M M, Wang M M, Yang J, Wen J, Guo P C, Wu Y W, Ke Y Z, Li P F, Li J N, Du H. 2019. Evolutionary and comparative expression analyses of TCP transcription factor gene family in land plants. International Journal of Molecular Sciences, 20 (14):3591. doi: 10.3390/ijms20143591 URL
[22]	Liu Xuan, Chen Yuchao, Guo Shenghu, Zhong Nan, Shi Lei, Gan Xiaoyan. 2024. Gene identification and expression analysis of the TCP gene family of Arundo donax under salt stress. Pratacultural Science, 41 (10):2316-2329. (in Chinese)
	刘璇, 陈虞超, 郭生虎, 钟楠, 石磊, 甘晓燕. 2024. 芦竹TCP家族的基因鉴定与盐胁迫下的表达分析. 草业科学, 41 (10):2316-2329.
[23]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-∆∆CT method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[24]	Niwa M, Daimon Y, Kurotani K, Higo A, Pruneda-Paz J L, Breton G, Mitsuda N, Kay S A, Ohme-Takagi M, Endo M, Araki T. 2013. BRANCHED1 interacts with FLOWERING LOCUS T to repress the floral transition of the axillary meristems in Arabidopsis. The Plant Cell, 25 (4):1228-1242. doi: 10.1105/tpc.112.109090 pmid: 23613197
[25]	Peng Ying. 2014. The research and utilization of the Eru cytoplasmic male sterility line in flowering Chinese cabbage(Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)[M. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	彭莹. 2014. 菜心(Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)Eru细胞质雄性不育系的研究与利用[硕士论文]. 武汉: 华中农业大学.
[26]	Poza-Carrión C, Aguilar-Martínez J A, Cubas P. 2007. Role of TCP gene BRANCHED1 in the control of shoot branching in Arabidopsis. Plant Signal Behav, 2 (6):551-552. doi: 10.4161/psb.2.6.4811 pmid: 19704556
[27]	Pruneda-Paz J L, Breton G, Para A, Kay S A. 2009. A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock. Science,323:1481-1485.
[28]	Rao Liping, Su Wenjin, Liu Yi, Song Tianxiao, Soviguidi Deka Reine Judess, Zhang Wenying, Yang Xinsun. 2020. Cloning and expression characteristics of chalcone isomerase gene IbCHI from Ipomoea batatas. Journal of Plant Resources and Environment, 29 (6):1-10. (in Chinese)
	饶莉萍, 苏文瑾, 刘意, 宋天晓, Soviguidi Deka Reine Judess, 张文英, 杨新笋. 2020. 甘薯查尔酮异构酶基因IbCHI的克隆和表达特性. 植物资源与环境学报, 29 (6):1-10.
[29]	Rameau C, Bertheloot J, Leduc N, Andrieu B, Foucher F, Sakr S. 2014. Multiple pathways regulate shoot branching. Frontiers in Plant Science,5:741.
[30]	Shang X, Han Z, Zhang D, Wang Y, Qin H, Zou Z, Zhou L, Zhu X, Fang W, Ma Y. 2022. Genome-wide analysis of the TCP gene family and their expression pattern analysis in tea plant(Camellia sinensis). Frontiers in Plant Science,13:840350.
[31]	Shen J, Ge D, Song X, Xiao J, Liu X, Che G, Gu R, Wang Z, Cheng Z, Song W, Liu L, Chen J, Han L, Yan L, Liu R, Zhou Z, Zhang X. 2021. Roles of CsBRC1-like in leaf and lateral branch development in cucumber. Plant Science,302:110681.
[32]	Shi Churan. 2022. Function analysis of flowering-related gene BcSPL15 and identification of BcTCP family in non-heading Chinese cabbage[M. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	史楚然. 2022. 不结球白菜开花相关基因BcSPL15的功能分析及BcTCP家族鉴定[硕士论文]. 南京: 南京农业大学.
[33]	Seale M, Bennett T, Leyser O. 2017. BRC1 expression regulates bud activation potential but is not necessary or sufficient for bud growth inhibition in Arabidopsis. Development, 144 (9):1661-1673.
[34]	Sun Q, Xie Y H, Li H M, Liu J L, Geng R, Wang P, Chu Z Y, Chang Y, Li G J, Zhang X, Yuan Y L, Cai Y F. 2022. Cotton GhBRC1 regulates branching,flowering,and growth by integrating multiple hormone pathways. The Crop Journal, 10 (1):75-87. doi: 10.1016/j.cj.2021.01.007 URL
[35]	Sun Qixue, Chen Zhuoting, Chen Rongrong, Zhang Bing. 2024. Genome-wide identification and analysis of TCP transcription factors in bermudagrass. Pratacultural Science, 41 (10):2330-2344. (in Chinese)
	孙启雪, 陈焯婷, 陈荣荣, 张兵. 2024. 狗牙根TCP转录因子基因家族鉴定与分析. 草业科学, 41 (10):2330-2344.
[36]	Sun Tian, Zhang Yinghua, Lü Xia, Peng Mengling, Xu Junqiang. 2021. Advances in molecular regulation mechanism of lateral branch development in plants. Plant Physiology Journal, 57 (8):1609-1616. (in Chinese)
	苏甜, 张应华, 吕霞, 彭梦玲, 倪雪梅, 许俊强. 2021. 植物侧枝发育的分子调控机理研究进展. 植物生理学报, 57 (8):1609-1616.
[37]	Wang Chong, Lan Mengjiao, Xiao Manqiu, Pan Hao, Deng Jiaqi, Wu Wensheng. 2025. Genome-wide identification and bioinformatics analysis of the PAL gene family in sweet potato[Ipomoea batatas(L.) Lam.]. Journal of Plant Genetic Resources, 26 (6):1091-1105. (in Chinese)
	王崇, 兰孟焦, 肖满秋, 潘皓, 邓佳祺, 吴问胜. 2025. 甘薯PAL基因家族的全基因组鉴定及生物信息学分析. 植物遗传资源学报, 26 (6):1091-1105.
[38]	Wang Shize, Li Yun, Han Yucui, Yu Shizhou, Wang Shuang, Liu Yong, Lin Xiaohu. 2024. Identification and expression analysis of TCP family members in tobacco(Nicotiana tabacum L.). Chinese Journal of Biotechnology, 40 (1):226-238. (in Chinese) doi: 10.13345/j.cjb.230345 pmid: 38258643
	王世泽, 李云, 韩玉翠, 余世洲, 王爽, 刘勇, 林小虎. 2024. 烟草TCP家族成员鉴定及表达分析. 生物工程学报, 40 (1):226-238. pmid: 38258643
[39]	Wang Wenlong, Liu Zhaokun, Lu Wenjun, Wang Yaolong, Li Xiaofeng, Zhu Hongfang, Liu Tongkun, Li Ying, Hou Xilin, Zhang Changwei. 2025. Enhancing ascorbic acid content in non-heading Chinese cabbage using gene editing technology. Acta Horticulturae Sinica, 52 (5):1317-1325. (in Chinese) doi: 10.16420/j.issn.0513-353x.2025-0036
	王文龙, 刘照坤, 鲁文君, 王耀龙, 李晓锋, 朱红芳, 刘同坤, 李英, 侯喜林, 张昌伟. 2025. 利用基因编辑技术提高不结球白菜抗坏血酸的含量. 园艺学报, 52 (5):1317-1325. doi: 10.16420/j.issn.0513-353x.2025-0036
[40]	Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J H, Bancroft I, Cheng F. 2011. The genome of the mesopolyploid crop species Brassica rapa. Nat Genet, 43 (10):1035-1039. doi: 10.1038/ng.919 pmid: 21873998
[41]	Wu Dongxiong. 2017. Identification of TCP gene family and the initial establishment of CRISPR-Cas9 gene editing system in Brassica rapa chinensis[M. D. Dissertation]. Fujian: Fujian Agriculture and Forestry University. (in Chinese)
	吴栋雄. 2017. 白菜TCP基因家族的鉴定及利用CRISPR-Cas9系统进行编辑的初步探索[硕士论文]. 福建: 福建农林大学.
[42]	Yan Min, Wang Han, Xu Ye, Liu Shaohua, Liu Haoqi. 2024. Genome-wide identification and expression analysis of BrRopGEF gene family in Chinese cabbage. Molecular Plant Breeding, 22 (22):7347-7354. (in Chinese)
	闫敏, 王晗, 许晔, 刘少华, 刘皓琦. 2024. 大白菜BrRopGEF基因家族全基因组鉴定及表达分析. 分子植物育种, 22 (22):7347-7354.
[43]	Yang Wenlong, Xu Chu, Han Jiaqi, Zhang Xiaohui, Song Jiangping, Jia Huixia, Wang Haiping. 2023. Genome-wide identification and characterization of the WOX gene family in Brassica juncea. Chinese Journal of Biotechnology, 39 (2):537-551. (in Chinese) doi: 10.13345/j.cjb.220774 pmid: 36847088
	阳文龙, 徐楚, 韩嘉琪, 张晓辉, 宋江萍, 贾会霞, 王海平. 2023. 芥菜WOX基因家族的全基因组鉴定与分析. 生物工程学报, 39 (2):537-551. pmid: 36847088
[44]	Yang Y, Nicolas M, Zhang J, Yu H, Guo D, Yuan R, Zhang T, Yang J, Cubas P, Qin G. 2018. The TIE1 transcriptional repressor controls shoot branching by directly repressing BRANCHED1 in Arabidopsis. PLoS Genet, 14 (3):e1007296.
[45]	Yao X, Ma H, Wang J, Zhang D B. 2007. Genome-wide comparative analysis and expression pattern of TCP gene families in Arabidopsis thaliana and Oryza sativa. J Integr Plant Biol,49:885-897.
[46]	Zhang Xin, Li Minmin, Zhao Yang, Sun Yunchao, Ji Chuanyun, Cheng Qianqian, Zhang Lanying. 2024. Genome-wide identification and expression analysis of AP2/ERF gene family in hexaploid cultivated wheat. Jiangsu Agricultural Sciences, 52 (16):58-68. (in Chinese)
	张新, 李敏敏, 赵杨, 孙允超, 冀传允, 程倩倩, 张兰迎. 2024. 六倍体栽培小麦AP2/ERF基因家族的全基因组鉴定及表达分析. 江苏农业科学, 52 (16):58-68.
[47]	Zhang Ying, Li Haijun, Gao Fucheng, Mu Xiaoguo, Gao Hu, Wu Xue, Ye Lin, Cao Kai. 2023. Screening of salt-tolerant cultivars of Brassica campestris L. and its salt-tolerant mechanism. Acta Agriculturae Boreali-Sinica, 38 (S1):170-179. (in Chinese)
	张莹, 李海俊, 高富成, 穆晓国, 高虎, 吴雪, 叶林, 曹凯. 2023. 菜心耐盐品种的筛选及其耐盐机理. 华北农学报, 38 (S1):170-179. doi: 10.7668/hbnxb.20194042
[48]	Zheng Ling, Bai Xueting, Li Huiyun. 2019. Genome-wide identification and expression analysis of TCP gene family in Sorghum bicolor L. Journal of Henan Agricultural Sciences, 48 (10):30-36. (in Chinese)
	郑玲, 白雪婷, 李会云. 2019. 高粱TCP基因家族全基因组鉴定及表达分析. 河南农业科学, 48 (10):30-36.
[49]	Zhou Erxun, Yang Mei, Zhang Hua, Liu Zizhu. 2002. Factors affecting the mycelial growth,sporulation and conidial germination of Colletotrichum higginsianum Sacc. Journal of Nanjing Agricultural University, 25 (2):47-51. (in Chinese)
	周而勋, 杨媚, 张华, 刘自珠. 2002. 菜心炭疽病菌菌丝生长、产孢和孢子萌发的影响因素. 南京农业大学学报, 25 (2):47-51.
[50]	Zou Q, Dong Q, Tian D, Mao L, Cao X, Zhu K. 2023. Genome-wide analysis of TCP transcription factors and their expression pattern analysis of rose plants(Rosa chinensis). Current Issues in Molecular Biology, 45 (8):6352-6364. doi: 10.3390/cimb45080401 URL

基因编号 Gene ID	正向引物序列（5′-3′） Forward primer	反向引物序列（5′-3′） Reverse primer
BraA01g009870.3C	GCTTCCTTCCCTCAACAACACGAAT	GGCTGTTCTCTCTCTAGCTCTCTCC
BraA01g034360.3C	ATTATTGCTCACAACCATTACTCCC	GATGGTCAAGATGATTGTTTGATGT
BraA01g036950.3C	ACCGTCCGAGCAAAGCCGTTGACTG	GGCGACGGAGAGATTTTAGTTTTGG
BraA02g002690.3C	CGCCGTCAAAAAACCACCGACCAAG	GTGGCGGCTATGATGGAAGGCTCGG
BraA02g015610.3C	CCCAAACAAGCAACAGGAAGTCGGG	TCCTCCGACCACGTCCCTCTACTTT
BraA02g017550.3C	TAATCCCTACAATCATCAGTCAACA	TCATAACCTTTCTGATTCACAGCAC
BraA02g018710.3C	TCTTTCACTACTCATATCCTAAGCC	CCAGTTCTTCTTGGTGAAACCTTTT
BraA02g019350.3C	TGGCTCATCCTCATCAGGTGCGTCC	ATCCAGCCGTTGATTGAACTAAATA
BraA02g020650.3C	CCGCTAGAGTTTTCCAGTTGACGAG	GCGTAAAGTAGCGTTTAAAGTCGAG
BraA02g042560.3C	CTGAGCCTTCCATCATAGCCGCAAC	GGTATAGCACCTCCGAAACCCTCTC
BraA02g042770.3C	CAGAATCAGCCAAGTTCAGTACCAA	TTTGCTGTGTCTGTCTTTGCCACCA
BraA03g003300.3C	CGATTCAGCTCTACGACCTTCAAGA	TTCAAACCTTCTCTCTGGATCGTAT
BraA03g003460.3C	CACAATGGGGGCTTCTTTGATGAGT	GCCAGAAGATTGAGATGATGACCCG
BraA03g023810.3C	CAATAGGACCGCCACTGAAGAGAGC	GGCGTTCTCCAACAACCACCGAATC
BraA03g030780.3C	CTTTCACTCTTTTCAACCATCATCA	CACCTCTCTCATTGTTGTTTTCTTC
BraA03g036760.3C	TTTCTCCATCTCCTCCGCCGCAGCA	TGGTTATTATCATTACTCCGACGTT
BraA03g038400.3C	AACTCCAACATATCAAGCAATCCTC	GTTCAAGGGAAGAAGTGATGGATGA
BraA03g043950.3C	AGGTTGTCCGTTCCAACAGCAATTC	GGAAATTGTAATGGAGGGAGCTTGT
BraA03g048240.3C	CTAACTGGTGCTTCTCCTACCGCTG	CTCTCCTTGGTCTCTTTAGCTGTTC
BraA04g013610.3C	AATCAAACCCGCCACCGACTCCGTT	ACCAAACCCAGTCTCATTTTCCCAA
BraA05g005380.3C	CCGACGAACCAGCCTCAGATGTTAG	CTCCTGTTTCTCGTAAATCTCCAGC
BraA05g013580.3C	ATCTTCTTCTTCCGAGCCCAACTTG	ACCGCCGCTTGTAGTTGTTGTCGCC
BraA05g032060.3C	GAAACCGAGAAGAACCGCCGCTAAA	CTCGTTCTCGTTCCCCCGATGCTCC
BraA05g041050.3C	AAAATTAATGGATGTGTAGAAGAGT	GTTGATGCTGTTCCATGTATTGATA
BraA06g019370.3C	ACTTCACTTCTCTCAACATCTCTCT	GTTGCTGTTGAAGTAGCGATGGAGA
BraA06g030710.3C	AAGCTCCACCACTATTTCCACTTCC	GGCGTAGCAAAAGATCCCATGTCGT
BraA06g037920.3C	CATCTTCAGGGTGGTGGGTCTCTCA	CTCCATCTCCCCCGCCAACACCCCA
BraA07g030260.3C	AGTCACATTCTTCTTCTTCTTCGCA	CGTATTATCACCACTGCTCCAAAAC
BraA07g031420.3C	TTTCTCGCCAATTCTTCGATCTCCA	CCATCGTTTCCAAAATCTTCAACAT
BraA07g032600.3C	TCAAGAAGGCGGTGAAGAAAGATAG	GTCTAATGTTTTACTGGCTTTGTCA
BraA07g034590.3C	CTCTCTCTCTGCTGCTCATCTTCGT	AGAAGAAGAACAAGAATCCGACTCA
BraA07g036490.3C	ATGTCGTTTCAGGGTAGTGGAATGT	GGATTTCTCTCTGCCGACATTATAG
BraA08g001700.3C	GGCTATTCAGTTCTACGATGTTCAA	TTTTGGGTCTTTTGGGTTTGTTTGC
BraA08g023460.3C	AGCCGTCGTTGAATCATAGTAATAG	GAGAGGTAGTGAATTGGACTGAAGG
BraA09g003050.3C	AAGACGACAATCATCATCACAACAG	CTTTGGTGTGTCTGTCTTTGTTTGA
BraA09g006660.3C	TTACAACAACAGCAGCAACAAGCAG	GCTCCACTAGATAAAGAAGCAAGCA
BraA09g018190.3C	CACCATCAGTACGAGGAGCAAGGAG	TGTTGATGATGTTGCTGTTGTTGAT
BraA09g034850.3C	TCTTCCTCTTCTCTACATCATTACA	GAGAATGTGAATTGGACTGAAGGGT
BraA10g030640.3C	GTCAAGCCGAGCCTTCTATCATAGC	TGTGTCCAACCGATAATTCACCACC

基因编号 Gene ID	正向引物序列（5′-3′） Forward primer	反向引物序列（5′-3′） Reverse primer
BraA01g009870.3C	GCTTCCTTCCCTCAACAACACGAAT	GGCTGTTCTCTCTCTAGCTCTCTCC
BraA01g034360.3C	ATTATTGCTCACAACCATTACTCCC	GATGGTCAAGATGATTGTTTGATGT
BraA01g036950.3C	ACCGTCCGAGCAAAGCCGTTGACTG	GGCGACGGAGAGATTTTAGTTTTGG
BraA02g002690.3C	CGCCGTCAAAAAACCACCGACCAAG	GTGGCGGCTATGATGGAAGGCTCGG
BraA02g015610.3C	CCCAAACAAGCAACAGGAAGTCGGG	TCCTCCGACCACGTCCCTCTACTTT
BraA02g017550.3C	TAATCCCTACAATCATCAGTCAACA	TCATAACCTTTCTGATTCACAGCAC
BraA02g018710.3C	TCTTTCACTACTCATATCCTAAGCC	CCAGTTCTTCTTGGTGAAACCTTTT
BraA02g019350.3C	TGGCTCATCCTCATCAGGTGCGTCC	ATCCAGCCGTTGATTGAACTAAATA
BraA02g020650.3C	CCGCTAGAGTTTTCCAGTTGACGAG	GCGTAAAGTAGCGTTTAAAGTCGAG
BraA02g042560.3C	CTGAGCCTTCCATCATAGCCGCAAC	GGTATAGCACCTCCGAAACCCTCTC
BraA02g042770.3C	CAGAATCAGCCAAGTTCAGTACCAA	TTTGCTGTGTCTGTCTTTGCCACCA
BraA03g003300.3C	CGATTCAGCTCTACGACCTTCAAGA	TTCAAACCTTCTCTCTGGATCGTAT
BraA03g003460.3C	CACAATGGGGGCTTCTTTGATGAGT	GCCAGAAGATTGAGATGATGACCCG
BraA03g023810.3C	CAATAGGACCGCCACTGAAGAGAGC	GGCGTTCTCCAACAACCACCGAATC
BraA03g030780.3C	CTTTCACTCTTTTCAACCATCATCA	CACCTCTCTCATTGTTGTTTTCTTC
BraA03g036760.3C	TTTCTCCATCTCCTCCGCCGCAGCA	TGGTTATTATCATTACTCCGACGTT
BraA03g038400.3C	AACTCCAACATATCAAGCAATCCTC	GTTCAAGGGAAGAAGTGATGGATGA
BraA03g043950.3C	AGGTTGTCCGTTCCAACAGCAATTC	GGAAATTGTAATGGAGGGAGCTTGT
BraA03g048240.3C	CTAACTGGTGCTTCTCCTACCGCTG	CTCTCCTTGGTCTCTTTAGCTGTTC
BraA04g013610.3C	AATCAAACCCGCCACCGACTCCGTT	ACCAAACCCAGTCTCATTTTCCCAA
BraA05g005380.3C	CCGACGAACCAGCCTCAGATGTTAG	CTCCTGTTTCTCGTAAATCTCCAGC
BraA05g013580.3C	ATCTTCTTCTTCCGAGCCCAACTTG	ACCGCCGCTTGTAGTTGTTGTCGCC
BraA05g032060.3C	GAAACCGAGAAGAACCGCCGCTAAA	CTCGTTCTCGTTCCCCCGATGCTCC
BraA05g041050.3C	AAAATTAATGGATGTGTAGAAGAGT	GTTGATGCTGTTCCATGTATTGATA
BraA06g019370.3C	ACTTCACTTCTCTCAACATCTCTCT	GTTGCTGTTGAAGTAGCGATGGAGA
BraA06g030710.3C	AAGCTCCACCACTATTTCCACTTCC	GGCGTAGCAAAAGATCCCATGTCGT
BraA06g037920.3C	CATCTTCAGGGTGGTGGGTCTCTCA	CTCCATCTCCCCCGCCAACACCCCA
BraA07g030260.3C	AGTCACATTCTTCTTCTTCTTCGCA	CGTATTATCACCACTGCTCCAAAAC
BraA07g031420.3C	TTTCTCGCCAATTCTTCGATCTCCA	CCATCGTTTCCAAAATCTTCAACAT
BraA07g032600.3C	TCAAGAAGGCGGTGAAGAAAGATAG	GTCTAATGTTTTACTGGCTTTGTCA
BraA07g034590.3C	CTCTCTCTCTGCTGCTCATCTTCGT	AGAAGAAGAACAAGAATCCGACTCA
BraA07g036490.3C	ATGTCGTTTCAGGGTAGTGGAATGT	GGATTTCTCTCTGCCGACATTATAG
BraA08g001700.3C	GGCTATTCAGTTCTACGATGTTCAA	TTTTGGGTCTTTTGGGTTTGTTTGC
BraA08g023460.3C	AGCCGTCGTTGAATCATAGTAATAG	GAGAGGTAGTGAATTGGACTGAAGG
BraA09g003050.3C	AAGACGACAATCATCATCACAACAG	CTTTGGTGTGTCTGTCTTTGTTTGA
BraA09g006660.3C	TTACAACAACAGCAGCAACAAGCAG	GCTCCACTAGATAAAGAAGCAAGCA
BraA09g018190.3C	CACCATCAGTACGAGGAGCAAGGAG	TGTTGATGATGTTGCTGTTGTTGAT
BraA09g034850.3C	TCTTCCTCTTCTCTACATCATTACA	GAGAATGTGAATTGGACTGAAGGGT
BraA10g030640.3C	GTCAAGCCGAGCCTTCTATCATAGC	TGTGTCCAACCGATAATTCACCACC

基因编号 Gene ID	分子量/kD Molecular weight	等电点 Isoelectric point	不稳定性系数 Instability coefficient	平均亲水性系数 Hydrophilicity average coefficient
BraA01g009870.3C	39 219.70	7.91	42.74	-0.871
BraA01g034360.3C	47 318.52	6.77	49.63	-0.867
BraA01g036950.3C	38 203.22	6.81	75.02	-0.719
BraA02g002690.3C	23 842.65	9.57	49.44	-0.388
BraA02g015610.3C	30 182.49	5.50	56.51	-0.651
BraA02g017550.3C	39 308.67	5.50	48.92	-0.823
BraA02g018710.3C	33 911.64	6.03	58.48	-0.818
BraA02g019350.3C	33 870.94	7.12	59.49	-0.696
BraA02g020650.3C	39 098.21	8.63	43.51	-0.667
BraA02g042560.3C	18 600.96	7.92	54.69	-0.162
BraA02g042770.3C	33 796.16	6.50	47.54	-0.791
BraA03g003300.3C	27 367.45	6.49	51.36	-0.518
BraA03g003460.3C	24 263.25	10.18	37.89	-0.311
BraA03g023810.3C	33 174.90	9.67	55.70	-0.237
BraA03g030780.3C	31 309.72	6.50	52.25	-0.782
BraA03g036760.3C	44 499.00	7.49	67.30	-0.804
BraA03g038400.3C	45 530.80	6.76	56.68	-0.787
BraA03g043950.3C	40 698.22	6.18	56.00	-0.904
BraA03g048240.3C	42 262.93	7.12	45.39	-0.979
BraA04g013610.3C	24 724.61	7.20	36.43	-0.845
BraA05g005380.3C	34 510.13	9.58	57.82	-0.274
BraA05g013580.3C	38 727.41	6.34	50.44	-0.870
BraA05g032060.3C	44 173.53	7.00	63.12	-0.797
BraA05g041050.3C	34 451.32	7.21	56.70	-0.706
BraA06g019370.3C	50 238.37	6.59	67.54	-0.927
BraA06g030710.3C	26 991.12	9.69	57.09	-0.589
BraA06g037920.3C	32 446.81	7.25	55.21	-0.720
BraA07g030260.3C	33 733.76	7.43	56.69	-0.715
BraA07g031420.3C	39 407.14	6.28	49.11	-0.784
BraA07g032600.3C	38 800.34	6.36	57.19	-0.875
BraA07g034590.3C	34 066.09	6.91	60.33	-0.733
BraA07g036490.3C	37 206.26	6.84	45.51	-0.605
BraA08g001700.3C	37 595.34	6.74	66.60	-0.786
BraA08g023460.3C	34 410.52	6.61	64.29	-0.921
BraA09g003050.3C	29 671.62	6.09	41.97	-0.704
BraA09g006660.3C	23 116.85	9.38	44.70	-0.491
BraA09g018190.3C	41 385.31	6.09	54.72	-0.682
BraA09g034850.3C	35 327.53	7.80	51.70	-0.929
BraA10g030640.3C	24 733.42	8.89	45.27	-0.484

基因编号 Gene ID	分子量/kD Molecular weight	等电点 Isoelectric point	不稳定性系数 Instability coefficient	平均亲水性系数 Hydrophilicity average coefficient
BraA01g009870.3C	39 219.70	7.91	42.74	-0.871
BraA01g034360.3C	47 318.52	6.77	49.63	-0.867
BraA01g036950.3C	38 203.22	6.81	75.02	-0.719
BraA02g002690.3C	23 842.65	9.57	49.44	-0.388
BraA02g015610.3C	30 182.49	5.50	56.51	-0.651
BraA02g017550.3C	39 308.67	5.50	48.92	-0.823
BraA02g018710.3C	33 911.64	6.03	58.48	-0.818
BraA02g019350.3C	33 870.94	7.12	59.49	-0.696
BraA02g020650.3C	39 098.21	8.63	43.51	-0.667
BraA02g042560.3C	18 600.96	7.92	54.69	-0.162
BraA02g042770.3C	33 796.16	6.50	47.54	-0.791
BraA03g003300.3C	27 367.45	6.49	51.36	-0.518
BraA03g003460.3C	24 263.25	10.18	37.89	-0.311
BraA03g023810.3C	33 174.90	9.67	55.70	-0.237
BraA03g030780.3C	31 309.72	6.50	52.25	-0.782
BraA03g036760.3C	44 499.00	7.49	67.30	-0.804
BraA03g038400.3C	45 530.80	6.76	56.68	-0.787
BraA03g043950.3C	40 698.22	6.18	56.00	-0.904
BraA03g048240.3C	42 262.93	7.12	45.39	-0.979
BraA04g013610.3C	24 724.61	7.20	36.43	-0.845
BraA05g005380.3C	34 510.13	9.58	57.82	-0.274
BraA05g013580.3C	38 727.41	6.34	50.44	-0.870
BraA05g032060.3C	44 173.53	7.00	63.12	-0.797
BraA05g041050.3C	34 451.32	7.21	56.70	-0.706
BraA06g019370.3C	50 238.37	6.59	67.54	-0.927
BraA06g030710.3C	26 991.12	9.69	57.09	-0.589
BraA06g037920.3C	32 446.81	7.25	55.21	-0.720
BraA07g030260.3C	33 733.76	7.43	56.69	-0.715
BraA07g031420.3C	39 407.14	6.28	49.11	-0.784
BraA07g032600.3C	38 800.34	6.36	57.19	-0.875
BraA07g034590.3C	34 066.09	6.91	60.33	-0.733
BraA07g036490.3C	37 206.26	6.84	45.51	-0.605
BraA08g001700.3C	37 595.34	6.74	66.60	-0.786
BraA08g023460.3C	34 410.52	6.61	64.29	-0.921
BraA09g003050.3C	29 671.62	6.09	41.97	-0.704
BraA09g006660.3C	23 116.85	9.38	44.70	-0.491
BraA09g018190.3C	41 385.31	6.09	54.72	-0.682
BraA09g034850.3C	35 327.53	7.80	51.70	-0.929
BraA10g030640.3C	24 733.42	8.89	45.27	-0.484

Identification of the TCP Gene Family in Brassica campestris and Analysis of Their Effects on Axillary Bud Traits

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 50

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yanqiu, BAN Jingjie, ZENG Yuhan, LAI Chunwang, HUANG Xiaoling, ZHOU Jianjin, WAN Kui, LAI Zhongxiong, LIN Yuling. Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress [J]. Acta Horticulturae Sinica, 2026, 53(1): 159-173.
[2]	WANG Xinru, BAI Shuaishuai, SUN Yule, WU Xinyu, ZENG Feng, LIU Chunying, ZHANG Yuxi, GAI Shupeng, YUAN Yanchao. Genome-Wide Identification of PoSnRK Gene Family in Paeonia ostii and Expression Analysis During Flower Senescence [J]. Acta Horticulturae Sinica, 2025, 52(9): 2363-2376.
[3]	DONG Xuanke, LI Yingyang, MA Yuwan, CAI Yanfang, DING Qihan, CHEN Haixia, LI Yufan, and CHEN Jiren. The Functional Analysis of RcTCP9 Gene in Response to Salt and Drought Stress in Rosa chinensis‘Yueyuehong’ [J]. Acta Horticulturae Sinica, 2025, 52(7): 1789-1802.
[4]	YANG Chunmei, YU Yang, DING Yuge, XIA Jing, ZHOU Ling, and PENG Lei. Joint Transcription-Metabolism Analysis of Starch and Sucrose Metabolic Pathways in the Transformation of Axillary Buds into Flower Buds of ‘Guifei’Mango [J]. Acta Horticulturae Sinica, 2025, 52(6): 1412-1426.
[5]	CHEN Mindong, WANG Bin, BAI Changhui, QIU Boyin, LIU Jianting, WEN Qingfang, ZHU Haisheng, and LI Yongping. Identification of Expansin Family Gene and Its Expression Analysis During Fruit Enlargement in Sponge Gourd [J]. Acta Horticulturae Sinica, 2025, 52(6): 1488-1504.
[6]	SUN Tingzhen, SHU Qin, MA Wei, SHI Yuzi, ZHANG Meng, XIANG Chenggang, BO Kailiang, DUAN Ying, WANG Changlin. Identification and Phylogenetic Analysis of Gibberellin Oxidase Gene Family and Its Expression Pattern in Main Vine Growth in Cucurbita maxima [J]. Acta Horticulturae Sinica, 2025, 52(3): 603-622.
[7]	REN Xiliang, WANG Yuhong, GAO Tianyi, WANG Jie, HUANG Yunping. A New High-Quality Non-Heading Chinese Cabbage Cultivar‘Yongqing 8’ [J]. Acta Horticulturae Sinica, 2025, 52(2): 541-542.
[8]	HAN Hongwei, CHANG Yanan, LIU Huifang, ZHUANG Hongmei, XING Jiayi, ZHANG Xiaoda, ZULIPIYE · Abudumilike, WANG Hao, WANG Qiang. Identification of Cytochrome P450 Family Genes in Melon and Expression Analysis in Response to Light Intensity [J]. Acta Horticulturae Sinica, 2025, 52(12): 3271-3287.
[9]	SU Yanyan, WANG Yutong, JING Yanfu, XU Yaoguang, YU Yang, and XIE Hua. Identification of PpTRM Gene Family Members and Screening the Interacting PpTRMs with the Fruit Shape Regulator PpOFP1 in Peach [J]. Acta Horticulturae Sinica, 2025, 52(11): 2835-2845.
[10]	XIA Yan, HU Ruoqian, YI Guangquan, SUN Hongwen, HUANG Shuxin, CHI Zhuoheng, SHI Min, LIANG Guolu, JING Danlong, GUO Qigao. Characterization of Loquat bHLH Gene Family and Functional Analysis of EjbHLH104 on Flowering Regulation in Triploid Loquat [J]. Acta Horticulturae Sinica, 2025, 52(10): 2582-2596.
[11]	BIAN Shuanling, DENG Changrong, FAN Luze, WU Yanxun, REN Yanjing. Construction of Yeast Library and Identification of the BrrCDPK Gene Family of PSY Potential Interacting Proteins in Turnip [J]. Acta Horticulturae Sinica, 2025, 52(10): 2625-2641.
[12]	LIU Lu, WANG Meng, ZHENG Xiaomei, WANG Keqing, MIAO Ruyi, ZANG Qiaolu. Identification and Expression Analysis of CBF Gene Family of Artemisia argyi [J]. Acta Horticulturae Sinica, 2025, 52(10): 2703-2712.
[13]	WANG Shanshan, GUO Rui, HE Ling, WU Chunhong, CHEN Chanyou, WAN Heping, ZHAO Huixia. Identification of Long Cowpea Lhc Gene Family and Its Expression Analysis Under Salt Stress [J]. Acta Horticulturae Sinica, 2025, 52(1): 111-122.
[14]	LIU Jianhao, JING Yanfu, LIU Yuexin, XU Yaoguang, YU Yang, GE Xiuxiu, XIE Hua. Identification of Peach NAC Gene Family and Role of PpNAC050 in Promoting Fruit Fructose Accumulation [J]. Acta Horticulturae Sinica, 2024, 51(9): 1983-1996.
[15]	ZHAO Jiaying, ZENG Zhouting, CEN Xinying, SHI Jiaoqi, LI Xiaoxian, SHEN Xiaoxia, YU Zhenming. Genome-Wide Identification and Expression Analysis of CCO Gene Family in Dendrobium officinale During Flower Development [J]. Acta Horticulturae Sinica, 2024, 51(9): 2075-2088.