Genome-Wide Identification of PoSnRK Gene Family in Paeonia ostii and Expression Analysis During Flower Senescence

doi:10.16420/j.issn.0513-353x.2024-0993

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (9): 2363-2376.doi: 10.16420/j.issn.0513-353x.2024-0993

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Genome-Wide Identification of PoSnRK Gene Family in Paeonia ostii and Expression Analysis During Flower Senescence

WANG Xinru¹, BAI Shuaishuai², SUN Yule¹, WU Xinyu¹, ZENG Feng³, LIU Chunying¹, ZHANG Yuxi¹, GAI Shupeng¹^,^**(), YUAN Yanchao¹^,^**()

¹ College of Life Science， Qingdao Agricultural University，Qingdao， Shandong 266109， China
² Shandong Provincial Station of Rural Economic Management and Service， Shandong Provincial Department of Agriculture and Rural Affairs， Ji’nan 250100， China
³ Agricultural and Rural Bureau of Qihe County， Dezhou， Shandong 251199， China

Received:2024-12-26 Revised:2025-03-03 Online:2025-09-25 Published:2025-09-24
Contact: GAI Shupeng, YUAN Yanchao

Abstract

Abstract:

To investigate the members and characteristics of sucrose non-fermenting-1-related protein kinase（SnRK）gene family in Paeonia suffruticosa，bioinformatics methods were employed to identify the members，resulting in the identification of 22 PoSnRKs，including 3 SnRK1s，6 SnRK2s，and 13 SnRK3s（CIPKs）；conserved domain analysis revealed that all PoSnRK1 proteins possessed the Pkinase，UBA，and KA1 domains，PoSnRK2s possessed the Pkinase domain，and PoCIPKs possessed the Pkinase and NAF domains；conserved motif analysis identified Motif 15，Motif 17，and Motif 18 as characteristic motifs of the SnRK1 subfamily，Motif 16 and Motif 19 as characteristic motifs of the SnRK2 subfamily，and Motif 7，Motif 10，and Motif 14 as characteristic motifs of the CIPK subfamily. Collinearity analysis indicated that there was only one pair of collinear PoSnRKs，Po04_CIPK10 and Po01_CIPK14；cis-acting element analysis found that most PoSnRK promoters contained cis-elements responsive to hormones and transcription factor binding. Transcriptome expression profiles and qRT-PCR results showed that the expression level of Po02_SnRK2.8，Po01_SnRK1.1，Po01_CIPK6 and Po04_CIPK10 was consistent with the flower senescence，suggesting that these four genes played a role in the flower aging process of tree peony.

Key words: tree peony, sucrose non-fermenting-1-related protein kinase, gene family, flowers, senescence

WANG Xinru, BAI Shuaishuai, SUN Yule, WU Xinyu, ZENG Feng, LIU Chunying, ZHANG Yuxi, GAI Shupeng, YUAN Yanchao. Genome-Wide Identification of PoSnRK Gene Family in Paeonia ostii and Expression Analysis During Flower Senescence[J]. Acta Horticulturae Sinica, 2025, 52(9): 2363-2376.

Figures/Tables 10

Table 1 Primer information in this study

基因名称 Gene name	引物名称 Primer name	核苷酸序列（5′-3′） Nucleotide sequence
Po01_CIPK14	Po01_CIPK14-QF	GCCAGAGATCGGACATGGTT
Po01_CIPK14	Po01_CIPK14-QR	GCCAAATAAGGCGTTAGCCG
Po01_SnRK1.1	Po01_SnRK1.1-QF	TCATCTGGCAGCAGGTCTTC
Po01_SnRK1.1	Po01_SnRK1.1-QR	CACTTTACCAAATGCCCCGC
Po02_SnRK2.8	Po02_SnRK2.8-QF	TCCGAGTTTCCGAAGAGTGC
Po02_SnRK2.8	Po02_SnRK2.8-QR	CATCAGTGCTCTGGTGGCTT
Po04_CIPK10	Po04_CIPK10-QF	ACTGGCATGAGTGTGGCAAT
Po04_CIPK10	Po04_CIPK10-QR	CAGCTCACCACCCTTGACAT
Po01_CIPK6	Po01_CIPK6-QF	TGCAAGCAATCGGAGACACT
Po01_CIPK6	Po01_CIPK6-QR	TTCACCTCCTTGGCGACATC
PsActin	PsActin-QF	GTTCCAGCCATCACTAATCGG
PsActin	PsActin-QR	GAATAGACCCTCCAATCCAGACAC

Fig. 1 Phylogenetic tree of SnRK proteins in tree peony‘Fengdan’and Arabidopsis thaliana

Fig. 2 The conserved motifs and gene structure of PoSnRK in tree peony‘Fengdan’

Table 2 The physicochemical properties and subcellular location of PoSnRK in tree peony‘Fengdan’

转录本序号 Transcript ID	亚家族 Subfamily	氨基酸数 Number of amino acid	分子量/kD Molecular weight	等电点 pI	总平均亲水系数 Grand average of hydropathy		亚细胞定位 Subcellular location
Po01_SnRK1.1	SnRK1	571	65.12	9.29	-0.282		细胞膜Cell membrane
Po02_SnRK1.1	SnRK1	519	59.17	8.54	-0.211		质体Plastid
PoUn1_SnRK1.1	SnRK1	461	52.97	8.57	-0.086		细胞膜Cell membrane
Po04_SnRK2.3	SnRK2-III	363	41.13	4.89	-0.260		细胞核Nucleus
Po03_SnRK2.4	SnRK2-I	314	35.86	5.24	-0.607		细胞质Cytoplasm细胞核 Nucleus
Po03_SnRK2.6	SnRK2-III	363	41.39	4.92	-0.367		细胞核Nucleus
Po04_SnRK2.7	SnRK2-II	309	34.77	5.02	-0.264		细胞核Nucleus
Po02_SnRK2.8	SnRK2-II	297	33.76	5.76	-0.252		质体Plastid
Po04_SnRK2.8	SnRK2-II	331	37.85	5.51	-0.195		细胞膜Cell membrane
Po03_CIPK1	SnRK3	456	51.42	8.31	-0.366		细胞核Nucleus
Po02_CIPK3	SnRK3	491	56.23	7.54	-0.310		质体Plastid
Po03_CIPK4	SnRK3	445	48.81	8.79	-0.027		质体Plastid
Po03_CIPK5	SnRK3	648	72.37	8.67		-0.455		细胞核Nucleus
Po01_CIPK6	SnRK3	437	49.06	8.98	-0.331		细胞质Cytoplasm
Po05_CIPK6	SnRK3	428	48.25	9.35	-0.250		细胞质Cytoplasm
Po01_CIPK10	SnRK3	415	46.89	9.37	-0.290		细胞质Cytoplasm
Po04_CIPK10	SnRK3	531	60.87	8.79	-0.366		细胞核Nucleus
PoUn2035_CIPK10	SnRK3	422	47.95	9.35	-0.318		质体Plastid
PoUn267_CIPK10	SnRK3	447	50.60	8.98	-0.341		细胞膜Cell membrane
Po01_CIPK14	SnRK3	443	50.43	7.61		-0.313		质体Plastid
Po05_CIPK20	SnRK3	450	50.91	8.45	-0.426		细胞核Nucleus
Po02_CIPK21	SnRK3	469	52.62	5.77	-0.426		细胞质Cytoplasm 细胞核Nucleus

Fig. 4 The collinearity analysis between SnRK in Paeonia ostii and SnRK in Arabidopsis thaliana，Vitis vinifera，and Paeonia ludlowii

Fig. 3 The chromosomal location（A）and the collinearity analysis（B）of PoSnRK in tree peony‘Fengdan’

Fig. 5 cis-Acting elements in the promoter of PoSnRK ABA：Abscisic acid response element；Aux：Auxin response element；MeJA：Jasmonic acid response element；SA：Salicylic acid response element； GA：Gibberellin response element；Wound：Wound response element；Dehydration：Dehydration response element；Drought：Drought response element；Low-temperature：Low-temperature response element；Anoxic：Anoxic response element；Stress：Stress response element；Defense and stress：Defense and stress response element；Light：Light response element；Anaerobic：Anaerobic induction expression response element；Circadian：Circadian expression response element；Zein metabolism：Zein metabolism response element；Flavonoid biosynthesis：Flavonoid biosynthesis response element；Root：Root-specific expression response element；Seed：Seed-specific expression response element；Endosperm：Endosperm-specific expression response element；Endosperm-negative：Endosperm-specific negative expression response element；Meristem：Meristem expression-related response element；AP-1：Transcription factor AP-1 response element；MYB：Transcription factor MYB response element；MYC：Transcription factor MYC response element； Phytohormone：Phytohormone-related response element；Stress：Stress-related response element；Progress：Biological process-related response element；Tissue：Tissue-specific expression-related response element；Transcription factor：Transcription factor response element；In left panel，the numbers in the grid represent the number of cis-elements，and in right panel，the bar chart represents the number of cis-elements，of each category

Fig. 6 Expression profile analysis of PoSnRK during flower senescence in peony‘Xueying Taohua’ S1：Initial blooming stage；S2：Half-opening stage；S3：Full blooming stage；S4：Initial wilting stage；S5：Aging stage. The same below

Fig. 7 The relative expression level of PoSnRK during peony flower senescence Different lowercase letters indicate significant differences at different developmental stages，P < 0.05

Fig. 8 Prediction of transcription factors binding to PoSnRK promoters

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Genome-Wide Identification of PoSnRK Gene Family in Paeonia ostii and Expression Analysis During Flower Senescence

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