Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress

doi:10.16420/j.issn.0513-353x.2024-0860

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (1): 159-173.doi: 10.16420/j.issn.0513-353x.2024-0860

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress

WANG Yanqiu¹, BAN Jingjie¹, ZENG Yuhan¹, LAI Chunwang¹, HUANG Xiaoling¹, ZHOU Jianjin², WAN Kui¹, LAI Zhongxiong¹, LIN Yuling¹^,^*()

¹ Institute of Horticultural Biotechnology， Fujian Agriculture and Forestry University， Fuzhou 350002， China
² Sanming Agricultural science Research Institute， Sanming， Fujian 365051， China

Received:2025-03-09 Revised:2025-10-24 Online:2026-01-25 Published:2026-01-26
Contact: LIN Yuling

Abstract

Abstract:

To investigate the function of DREB（dehydration responsive element binding）gene family in Polygonatum cyrtonema in this study. The DREB family members were identified based on data including the full-length transcriptome and the transcriptome of different tissues and organs，and their protein physicochemical properties，phylogenetic characteristics，conserved motifs and protein structure were analyzed. The expression levels in different organs，salt stress and PEG-6000 simulated drought stress were analyzed by combining FPKM values and qRT-PCR. The subcellular localization of PcDREB10 was studied by Agrobacterium-mediated transformation of onion. The hairy roots of P. cyrtonema were induced by Agrobacterium rhizogenes，and the expression levels of PcDREB10/12/27 after overexpression of PcDREB10 under salt stress were analyzed by qRT-PCR. In this study，35 DREB family members were identified，named as PcDREB1-PcDREB35，all of which are hydrophilic proteins with low stability. The expression levels of PcDREB family members are inconsistent in different organs，with tissue specificity，and the expression levels are different under salt stress and drought stress. Subcellular localization results showed that PcDREB10 was located in the nucleus. After overexpression of PcDREB10 mediated by Agrobacterium rhizogenes，the expression of PcDREB10/27 were up-regulated while the expression of PcDREB12 was down-regulated in roots treated with high salt solution，indicating that PcDREB10/12/27 may play different roles in the process of P. cyrtonema resisting salt stress.

Key words: Polygonatum cyrtonema, DREB, gene family identification, salt stress, drought stress, expression analysis

WANG Yanqiu, BAN Jingjie, ZENG Yuhan, LAI Chunwang, HUANG Xiaoling, ZHOU Jianjin, WAN Kui, LAI Zhongxiong, LIN Yuling. Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress[J]. Acta Horticulturae Sinica, 2026, 53(1): 159-173.

Figures/Tables 13

References 34

[2]	Chen Cuirong. 2023. Development and application of SSR molecular markers of Polygonatum cyrtonema Hua sibiricum based on full-length transcriptome[M. D. Dissertation]. Fuzhou: Fujian Agriculture and Forestry University. (in Chinese)
	陈翠蓉. 2023. 基于全长转录组的福建多花黄精SSR分子标记开发与应用[硕士论文]. 福州: 福建农林大学.
[3]	Cheng C, An L K, Li F Z, Ahmad W, Aslam M, Haq M Z U, Yan Y X, Ahmad R M. 2023. Wide-range portrayal of AP2/ERF transcription factor family in maize(Zea mays L.) development and stress responses. Genes, 14 (1):194.
[4]	Chinese Pharmacopoeia Commission. 2022. Chinese Pharmacopoeia. Beijing: The Medicine Science and Technology Press of China. (in Chinese)
	国家药典委员会. 2022. 中华人民共和国药典. 北京: 中国医药科技出版社.
[5]	Cui Bo, Hao Ping’an, Liang Fang, Zhang Yan, Wang Ximeng, Li Junlin, Jiang Suhua, Xu Shenping. 2020. Cloning and expression analysis of AP2/ERF family gene from Phalaenopsis under low temperature. Acta Horticulturae Sinica, 47 (1):85-97. (in Chinese)
	崔波, 郝平安, 梁芳, 张燕, 王喜蒙, 李俊霖, 蒋素华, 许申平. 2020. 蝴蝶兰AP2/ERF 家族基因的克隆及在低温下表达特性分析. 园艺学报, 47 (1):85-97. doi: 10.16420/j.issn.0513-353x.2019-0091
[6]	Cui X W, Wang S Y, Cao H, Guo H, Li Y J, Xu F X, Zheng M M, Xi X Z, Han C C. 2018. A review:the bioactivities and pharmacological applications of Polygonatum sibiricum polysaccharides. Molecules, 23 (5):1170. doi: 10.3390/molecules23051170 URL
[7]	Dong C, Xi Y, Chen X L, Cheng Z M. 2021. Genome-wide identification of AP2/EREBP in Fragaria vesca and expression pattern analysis of the FvDREB subfamily under drought stress. BMC Plant Biology, 21 (1):295. doi: 10.1186/s12870-021-03095-2
[8]	Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubouzet E G, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. 2003. OsDREB genes in rice,Oryza sativa L.,encode transcription activators that function in drought-,high-salt- and cold-esponsive gene expression. The Plant Journal, 33 (4):751-763. doi: 10.1046/j.1365-313X.2003.01661.x URL
[9]	Feng K, Hou X L, Xing G M, Liu J X, Duan A Q, Xu Z S, Li M T, Zhuang J, Xiong A S. 2020. Advances in AP2/ERF super-family transcription factors in plant. Critical Reviews in Biotechnology, 40 (6):750-776. doi: 10.1080/07388551.2020.1768509 pmid: 32522044
[10]	Floden A, Schilling E E. 2018. Using phylogenomics to reconstruct phylogenetic relationships within tribe Polygonateae(Asparagaceae),with a special focus on Polygonatum. Molecular Phylogenetics and Evolution,129:202-213.
[11]	Han Q H, Chen K L, Yan D, Hao G L, Qi J L, Wang C M, Dirk Lynnette M A, Bruce D A, Gong J H, Wang J H, Zhao T Y. 2020. ZmDREB2A regulates ZmGH3.2 and ZmRAFS,shifting metabolism towards seed aging tolerance over seedling growth. The Plant Journal, 104 (1):268-282. doi: 10.1111/tpj.v104.1 URL
[12]	Han Y L, Cai M H, Zhang S Q, Chai J W, Sun M J, Wang Y W, Xie Q Y, Chen Y H, Wang H Z, Chen T. 2022. Genome-wide identification of AP2/ERF transcription factor family and functional analysis of DcAP2/ERF# 96 associated with abiotic stress in Dendrobium catenatum. International Journal of Molecular Sciences, 23 (21):13603. doi: 10.3390/ijms232113603 URL
[13]	Hassan S, Berk K, Aronsson H. 2021. Evolution and identification of DREB transcription factors in the wheat genome:modeling,docking and simulation of DREB proteins associated with salt stress. Journal of Biomolecular Structure & Dynamics, 40 (16):7191-7204.
[14]	Hichri I, Muhovski Y, Clippe A, Žižková E, Dobrev P I, Motyka V, Lutts S. 2016. SlDREB2,a tomato dehydration-responsive element-binding 2 transcription factor,mediates salt stress tolerance in tomato and Arabidopsis. Plant Cell Environ, 39 (1):62-79. doi: 10.1111/pce.v39.1 URL
[15]	Hu X, Liang J X, Wang W J, Cai C Y, Ye S W, Wang N N, Han F Y, Wu Y X, Zhu Q. 2023. Comprehensive genome-wide analysis of the DREB gene family in Moso bamboo(Phyllostachys edulis):evidence for the role of PeDREB28 in plant abiotic stress response. The Plant Journal, 116 (5):1248-1270. doi: 10.1111/tpj.v116.5 URL
[16]	Hu Ziang, Luo Ping, Xin Jingjing, Cui Yongyi. 2024. Identification of DREB gene family in Phalaenopsis aphrodite and its expression analysis under abiotic stresses. Journal of Agricultural Biotechnology, 32 (8):1715-1728. (in Chinese)
	胡子昂, 罗平, 辛静静, 崔永一. 2024. 蝴蝶兰DREB基因家族鉴定及非生物胁迫表达分析. 农业生物技术学报, 32 (8):1715-1728.
[17]	Izadi-Darbandi A, Alameldin H, Namjoo N, Ahmad K. 2023. Introducing sorghum DREB2 gene in maize(Zea mays L.) to improve drought and salinity tolerance. Biotechnol Appl Biochem, 70 (4):1480-1488. doi: 10.1002/bab.v70.4 URL
[18]	Jia Xin, Zeng Zhen, Chen Yue, Feng Hui, Lü Yingmin, Zhao Shiwei. 2022. Cloning and expression analysis of RcDREB2A gene in Rosa chinensis‘Old Blush’. Acta Horticulturae Sinica, 49 (9):1945-1956. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0794
	贾鑫, 曾臻, 陈月, 冯慧, 吕英民, 赵世伟. 2022. 月季‘月月粉’RcDREB2A的克隆与表达分析. 园艺学报, 49 (9):1945-1956. doi: 10.16420/j.issn.0513-353x.2021-0794
[19]	Jiang Yusong, Li Honglei, Li Zhexin, Xu Xiaoyu, Li Longyun, Huang Mengjun. 2022. Identification and expression analysis of the ZoWRKY family in stress responses based on transcriptome data of ginger(Zingiber officinale Roscoe). Crops, 11 (4):37-45. (in Chinese)
	姜玉松, 李洪雷, 李哲馨, 徐晓玉, 李隆云, 黄孟军. 2022. 基于转录组数据的生姜ZoWRKY基因家族鉴定及逆境响应分析. 作物杂志, 11 (4):37-45.
[20]	Li Q Y, Zhu P W, Yu X Q, Xu J Y, Liu G L. 2024. Physiological and molecular mechanisms of rice tolerance to salt and drought stress: advances and future directions. International Journal of Molecular Sciences, 25 (17):9404. doi: 10.3390/ijms25179404 URL
[21]	Liang Y, Li X, Zhang D, Gao B, Yang H, Wang Y, Guan K, Wood A J. 2017. ScDREB8,a novel A-5 type of DREB gene in the desert moss Syntrichia caninervis,confers salt tolerance to Arabidopsis. Plant Physiol Biochem,120:242-251.
[22]	Liu Z J, Yuan G X, Liu S, Jia J T, Cheng L Q, Qi D M, Shen S H, Peng X J, Liu G S. 2017. Identified of a novel cis-element regulating the alternative splicing of LcDREB2. Scientific Reports,7:46106.
[23]	Niu X, Luo T, Zhao H, Su Y, Ji W, Li H. 2020. Identification of wheat DREB genes and functional characterization of TaDREB3 in response to abiotic stresses. Gene,740:144514.
[24]	Prodjinoto H, Irakoze W, Gandonou C, Quinet M, Lutts S. 2023. Comparison between the impact of osmotic and NaCl treatments on the expression of genes coding for ion transporters in Oryza glaberrima Steud. PLoS ONE, 18 (11):e0290752. doi: 10.1371/journal.pone.0290752 pmid: 37967065
[25]	Sakuma Y, Liu Q, Joseph G, Dubouzet, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. 2002. DNA-binding specificity of the ERF/AP 2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression. Biochem Biophys Res Commun, 290 (3):998-1009. doi: 10.1006/bbrc.2001.6299 URL
[26]	Sheng S, Guo X Y, Wu C Z, Xiang Y C, Duan S H, Yang W Q, Li W R, Cao F C, Liu L H. 2022. Genome-wide identification and expression analysis of DREB genes in alfalfa(Medicago sativa)in response to cold stress. Plant Signaling & Behavior, 17 (1):2081420.
[27]	Song Yating, Han Liqun, Ma Kai, Zhao Yu, Liu Liqiang. 2023. Identification and expression characterization of DREB transcription factor family in walnut. Molecular Plant Breeding, https://link.cnki.net/urlid/46.1068.S.20231226.0912.002. (in Chinese)
	宋雅婷, 韩立群, 马凯, 赵钰, 刘立强. 2023. 核桃DREB转录因子家族的鉴定及表达特性分析. 分子植物育种, https://link.cnki.net/urlid/46.1068.S.20231226.0912.002.
[1]	Cao X, Xie H, Song M, Lu J, Ma P, Huang B, Wang M, Tian Y, Chen F, Peng J, Lang Z, Li G, Zhu J K. 2022. Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture. Innovation(Camb), 4 (1):100345.
[28]	Tang Kuangang, Dong Bo, Wen Xiaojun, Yin Yumei, Xue Min, Su Zixian, Wang Maoyan. 2021. Ectopic expression of the AmDREB1F gene from Ammopiptanthus mongolicus enhances stress tolerance of transgenic Arabidopsis. Chinese Journal of Bioengineering, 37 (12):4329-4341. (in Chinese)
	唐宽刚, 董博, 温小俊, 殷玉梅, 薛敏, 苏子先, 王茅雁. 2021. 异源表达蒙古沙冬青AmDREB1F基因提高转基因拟南芥的耐逆性. 生物工程学报, 37 (12):4329-4341.
[29]	Wang H Y, Zheng Y S, Zhou Q, Li Y, Liu K T, Hou X L. 2024. Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots. Horticultural Plant Journal, 10 (2):450-460. doi: 10.1016/j.hpj.2023.03.018 URL
[30]	Wang Shanshan. 2022. Microtuber in vitro morphogenesis and related gene analysis in Polygonatum cyrtonema[M. D. Dissertation]. Fuzhou: Fujian Agriculture and Forestry University. (in Chinese)
	王姗姗. 2022. 多花黄精微块茎的离体形态发生及相关基因的表达分析[硕士论文]. 福州: 福建农林大学
[31]	Wang Zewen, Yang Yiwei, Wang Pengfei, Zhang Jiancheng, Mu Xiaopeng, Fu Hongbo, Du Junjie. 2020. Identification and analysis of DREB gene family in Cerasus humilis. Plant Physiology Journal, 56 (3):413-422. (in Chinese)
	汪泽文, 杨依维, 王鹏飞, 张建成, 穆霄鹏, 付鸿博, 杜俊杰. 2020. 欧李DREB基因家族的鉴定与分析. 植物生理学报, 56 (3):413-422.
[32]	Yu W L, Yang L, Xiang Y Y, Li R D, Zhou X Q, Gan L C, Xiang X Y, Zhang Y Y, Yuan L, Luo Y Q, Li G Z, Wang Y N, Chen Y H, Chen P, and Zhang C Y. 2024. Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae. Horticultural Plant Journal, 10 (4):1049-1060. doi: 10.1016/j.hpj.2024.05.002 URL
[33]	Yang Yang, Ye Bihuan, Song Qiyan, Chen Youwu, Hu Chuanjiu, Du Guojian, Liao Rongjun, Li Haibo. 2020. Selection and validation of internal reference genes for qPCR in Polygonatum sibiricum tubers at different development stages and in response to abiotic stress. China Medicine and Pharmacy, 45 (24):5967-5975. (in Chinese)
	杨阳, 叶碧欢, 宋其岩, 陈友吾, 胡传久, 杜国坚, 廖荣俊, 李海波. 2020. 多花黄精块茎发育和胁迫条件下qPCR内参基因的筛选与验证. 中国中药杂志, 45 (24):5967-5975.
[34]	Yang Z Y, Yang L F, Liu C K, Qin X J, Liu H Y, Chen J H, Ji Y H. 2019. Transcriptome analyses of Paris polyphylla var. chinensis,Ypsilandra thibetica,and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway. Fitoterapia,135:52-63.

用途 Purpose	引物名称 Primer name	引物序列（5′-3′） Primer sequence
载体构建Vector construction	PcDREB10-F	ACGGGGGACTCTTGACCATGGATGGAACCTATTCGAAACAACGAC
	PcDREB10-R	AAGTTCTTCTCCTTTACTAGTAAACCCCCATGTCCACTGAAATG
	GFP-F	AACATACGGAAAACTTACCCT
	GFP-R	ATAATGATCAGCGAGTTGCAC
实时荧光定量PCR	SYBR-PcDREB10-qF	CTGGGAAGGAGAAGCCTAAG
qRT-PCR	SYBR-PcDREB10-qR	CCCGTGAAATGCCTGTAA
	SYBR-PcDREB12-qF	GGCTTCACTCTATGCTCTG
	SYBR-PcDREB12-qR	TGGGCTTTCTTGGTTCTC
	SYBR-PcDREB27-qF	AGGGCATCGTCGCTCTAT
	SYBR-PcDREB27-qR	CCTGGTTCGGTTCTTTGG
	UBQ-qF	GGACCCAGAAGTACGCAATG
	UBQ-qR	AATTACCAGGGATACAGCACC

用途 Purpose	引物名称 Primer name	引物序列（5′-3′） Primer sequence
载体构建Vector construction	PcDREB10-F	ACGGGGGACTCTTGACCATGGATGGAACCTATTCGAAACAACGAC
	PcDREB10-R	AAGTTCTTCTCCTTTACTAGTAAACCCCCATGTCCACTGAAATG
	GFP-F	AACATACGGAAAACTTACCCT
	GFP-R	ATAATGATCAGCGAGTTGCAC
实时荧光定量PCR	SYBR-PcDREB10-qF	CTGGGAAGGAGAAGCCTAAG
qRT-PCR	SYBR-PcDREB10-qR	CCCGTGAAATGCCTGTAA
	SYBR-PcDREB12-qF	GGCTTCACTCTATGCTCTG
	SYBR-PcDREB12-qR	TGGGCTTTCTTGGTTCTC
	SYBR-PcDREB27-qF	AGGGCATCGTCGCTCTAT
	SYBR-PcDREB27-qR	CCTGGTTCGGTTCTTTGG
	UBQ-qF	GGACCCAGAAGTACGCAATG
	UBQ-qR	AATTACCAGGGATACAGCACC

家族 Family	基因 Gene	氨基酸数 Number of amino acid	分子质量/kD Molecular weight	等电点 pI	不稳定系数 Instability index	脂肪系数 Aliphatic index	总平均吸水性 Grand average of hydropathicity	亚细胞定位 Subcellular location
DREB-A1	PcDREB1	214	23.37	5.52	68.13	57.66	-0.545	细胞核 Nucleus
	PcDREB2	209	23.09	5.22	66.52	62.25	-0.517	细胞核 Nucleus
	PcDREB3	214	23.52	5.21	68.13	57.66	-0.574	细胞核 Nucleus
	PcDREB4	214	23.39	5.34	68.50	57.66	-0.550	细胞核 Nucleus
	PcDREB5	179	19.56	6.17	43.82	66.65	-0.430	细胞核 Nucleus
DREB-A2	PcDREB6	299	33.25	4.98	56.50	54.85	-0.906	细胞核 Nucleus
	PcDREB7	248	27.33	4.47	46.51	55.89	-0.779	细胞核 Nucleus
DREB-A2	PcDREB8	314	34.80	4.88	54.29	50.67	-0.971	细胞核 Nucleus
	PcDREB9	263	28.88	4.38	44.73	50.84	-0.863	细胞核 Nucleus
	PcDREB10	199	21.47	7.51	38.44	58.44	-0.458	细胞核 Nucleus
	PcDREB11	207	22.68	8.28	67.04	75.94	-0.478	细胞核 Nucleus
	PcDREB12	407	44.28	4.93	72.15	64.10	-0.426	细胞核 Nucleus
	PcDREB13	282	31.01	5.80	45.51	61.28	-0.526	细胞核 Nucleus
	PcDREB14	246	27.03	4.44	47.31	54.80	-0.620	细胞核 Nucleus
	PcDREB15	297	33.18	5.38	46.42	49.63	-0.895	细胞核 Nucleus
	PcDREB16	246	26.96	4.37	45.59	53.62	-0.651	细胞核 Nucleus
	PcDREB17	246	26.96	4.37	45.59	53.62	-0.651	细胞核 Nucleus
DREB-A4	PcDREB18	285	32.26	8.86	61.60	87.61	-0.212	叶绿体 Chloroplast
	PcDREB19	199	21.56	5.22	47.96	62.86	-0.384	细胞核 Nucleus
	PcDREB20	221	23.88	5.76	36.51	64.07	-0.534	细胞核 Nucleus
	PcDREB21	222	23.99	5.77	40.04	62.48	-0.528	细胞核 Nucleus
	PcDREB22	224	24.08	5.01	53.18	60.18	-0.546	细胞核 Nucleus
DREB-A5	PcDREB23	195	21.08	4.74	49.45	58.72	-0.371	细胞质Cytoplasm
	PcDREB24	172	18.48	5.95	59.10	72.15	-0.626	细胞核 Nucleus
	PcDREB25	172	18.51	6.61	57.28	61.40	-0.684	细胞核 Nucleus
	PcDREB26	202	21.68	5.78	54.66	59.11	-0.427	叶绿体 Chloroplast
DREB-A6	PcDREB27	481	52.26	8.60	61.01	49.88	-0.776	细胞核 Nucleus
	PcDREB28	482	52.40	7.95	61.51	50.58	-0.762	细胞核 Nucleus
	PcDREB29	481	52.26	8.60	61.01	49.88	-0.776	细胞核 Nucleus
	PcDREB30	352	39.06	6.66	59.27	74.06	-0.442	细胞核 Nucleus
	PcDREB31	354	38.74	5.86	68.70	72.60	-0.425	细胞核 Nucleus
	PcDREB32	422	47.14	5.84	51.95	77.01	-0.564	细胞核 Nucleus
	PcDREB33	365	40.10	9.22	63.35	72.16	-0.511	叶绿体 Chloroplast
	PcDREB34	353	39.12	7.05	58.53	76.06	-0.446	细胞核 Nucleus
	PcDREB35	308	34.73	5.80	63.56	71.98	-0.644	叶绿体 Chloroplast

家族 Family	基因 Gene	氨基酸数 Number of amino acid	分子质量/kD Molecular weight	等电点 pI	不稳定系数 Instability index	脂肪系数 Aliphatic index	总平均吸水性 Grand average of hydropathicity	亚细胞定位 Subcellular location
DREB-A1	PcDREB1	214	23.37	5.52	68.13	57.66	-0.545	细胞核 Nucleus
	PcDREB2	209	23.09	5.22	66.52	62.25	-0.517	细胞核 Nucleus
	PcDREB3	214	23.52	5.21	68.13	57.66	-0.574	细胞核 Nucleus
	PcDREB4	214	23.39	5.34	68.50	57.66	-0.550	细胞核 Nucleus
	PcDREB5	179	19.56	6.17	43.82	66.65	-0.430	细胞核 Nucleus
DREB-A2	PcDREB6	299	33.25	4.98	56.50	54.85	-0.906	细胞核 Nucleus
	PcDREB7	248	27.33	4.47	46.51	55.89	-0.779	细胞核 Nucleus
DREB-A2	PcDREB8	314	34.80	4.88	54.29	50.67	-0.971	细胞核 Nucleus
	PcDREB9	263	28.88	4.38	44.73	50.84	-0.863	细胞核 Nucleus
	PcDREB10	199	21.47	7.51	38.44	58.44	-0.458	细胞核 Nucleus
	PcDREB11	207	22.68	8.28	67.04	75.94	-0.478	细胞核 Nucleus
	PcDREB12	407	44.28	4.93	72.15	64.10	-0.426	细胞核 Nucleus
	PcDREB13	282	31.01	5.80	45.51	61.28	-0.526	细胞核 Nucleus
	PcDREB14	246	27.03	4.44	47.31	54.80	-0.620	细胞核 Nucleus
	PcDREB15	297	33.18	5.38	46.42	49.63	-0.895	细胞核 Nucleus
	PcDREB16	246	26.96	4.37	45.59	53.62	-0.651	细胞核 Nucleus
	PcDREB17	246	26.96	4.37	45.59	53.62	-0.651	细胞核 Nucleus
DREB-A4	PcDREB18	285	32.26	8.86	61.60	87.61	-0.212	叶绿体 Chloroplast
	PcDREB19	199	21.56	5.22	47.96	62.86	-0.384	细胞核 Nucleus
	PcDREB20	221	23.88	5.76	36.51	64.07	-0.534	细胞核 Nucleus
	PcDREB21	222	23.99	5.77	40.04	62.48	-0.528	细胞核 Nucleus
	PcDREB22	224	24.08	5.01	53.18	60.18	-0.546	细胞核 Nucleus
DREB-A5	PcDREB23	195	21.08	4.74	49.45	58.72	-0.371	细胞质Cytoplasm
	PcDREB24	172	18.48	5.95	59.10	72.15	-0.626	细胞核 Nucleus
	PcDREB25	172	18.51	6.61	57.28	61.40	-0.684	细胞核 Nucleus
	PcDREB26	202	21.68	5.78	54.66	59.11	-0.427	叶绿体 Chloroplast
DREB-A6	PcDREB27	481	52.26	8.60	61.01	49.88	-0.776	细胞核 Nucleus
	PcDREB28	482	52.40	7.95	61.51	50.58	-0.762	细胞核 Nucleus
	PcDREB29	481	52.26	8.60	61.01	49.88	-0.776	细胞核 Nucleus
	PcDREB30	352	39.06	6.66	59.27	74.06	-0.442	细胞核 Nucleus
	PcDREB31	354	38.74	5.86	68.70	72.60	-0.425	细胞核 Nucleus
	PcDREB32	422	47.14	5.84	51.95	77.01	-0.564	细胞核 Nucleus
	PcDREB33	365	40.10	9.22	63.35	72.16	-0.511	叶绿体 Chloroplast
	PcDREB34	353	39.12	7.05	58.53	76.06	-0.446	细胞核 Nucleus
	PcDREB35	308	34.73	5.80	63.56	71.98	-0.644	叶绿体 Chloroplast

Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress

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