Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

doi:10.16420/j.issn.0513-353x.2025-0225

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (5): 1343-1356.doi: 10.16420/j.issn.0513-353x.2025-0225

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

CAO Lei¹, DU Ping², YANG Riling¹, ZHOU Yuhao¹, HOU Juan¹, LI Xiang¹, WANG Panqiao¹, LI Lili¹, LUO Chen¹, LI Qiong¹, MAO Wenwen¹^,^**(), HU Jianbin¹^,^**()

¹ Research Center of Cucurbit Germplasm Enhancement and Utilization of Henan Province， College of Horticulture，Henan Agricultural University， Zhengzhou 450046， China
² Research Institute of Non-timber Forestry， Chinese Academy of Forestry， Zhengzhou 450003， China

Received:2025-12-29 Revised:2026-03-19 Online:2026-05-26 Published:2026-05-26
Contact: MAO Wenwen, HU Jianbin

Abstract

Abstract:

To investigate the role of the TIFY gene family in the low temperature stress response of melon（Cucumis melo L.），genome-wide identification of TIFY family members was performed using bioinformatics approaches. Tissue-specific expression patterns and low temperature stress responsive profiles were further analyzed through transcriptome sequencing and quantitative reverse transcription polymerase chain reaction technology. The results demonstrated that a total of 15 TIFY family genes were identified in melon，classified into four subfamilies：JAZ，TIFY，ZIM，and PPD，and distributed across nine chromosomes. Tissue-specific expression analysis revealed that CmTIFY genes were expressed in various tissues. Among them，CmTIFY5A，CmTIFY5B，CmTIFY5C，CmTIFY5D and CmTIFY9B exhibited higher expression levels in flowers compared to other tissues. With fruit ripening，the gene expression level of CmTIFY5B gradually increased，whereas that of CmTIFY6B gradually decreased. CmTIFY5B and CmTIFY6B may be involved in the regulation of melon fruit ripening and quality formation. The expression levels of most CmTIFY genes were up regulated under low temperature stress，among which CmTIFY5B and CmTIFY5C were most significantly up regulated under low temperature.

Key words: melon, abiotic stress, TIFY, gene family, expression analysis, low temperature stress

CAO Lei, DU Ping, YANG Riling, ZHOU Yuhao, HOU Juan, LI Xiang, WANG Panqiao, LI Lili, LUO Chen, LI Qiong, MAO Wenwen, HU Jianbin. Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon[J]. Acta Horticulturae Sinica, 2026, 53(5): 1343-1356.

Figures/Tables 8

References 55

[1]	An J P, Wang X F, Zhang X W, You C X, Hao Y J. 2021. Apple B-box protein BBX37 regulates jasmonic acid mediated cold tolerance through the JAZ-BBX37-ICE1-CBF pathway and undergoes MIEL1-mediated ubiquitination and degradation. New Phytologist, 229 (5):2707-2729. doi: 10.1111/nph.v229.5 URL
[2]	Ba L J, Kuang J F, Chen J Y, Lu W J. 2016. MaJAZ1 attenuates the MaLBD5-mediated transcriptional activation of jasmonate biosynthesis gene MaAOC2 in regulating cold tolerance of banana fruit. Journal of Agricultural and Food Chemistry, 64 (4):738-745. doi: 10.1021/acs.jafc.5b05005 URL
[3]	Bai Y, Meng Y, Huang D, Qi Y, Chen M. 2011. Origin and evolutionary analysis of the plant-specific TIFY transcription factor family. Genomics, 98 (2):128-136 doi: 10.1016/j.ygeno.2011.05.002 pmid: 21616136
[4]	Chayut N, Yuan H, Ohali S, Meir A, Yeselson Y, Portnoy V, Zheng Y, Fei Z, Lewinsohn E, Katzir N, Schaffer A A, Gepstein S, Burger J, Li L, Tadmor Y. 2015. A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with orange allelic variation and fruit β-carotene accumulation in melon fruit. BMC Plant Biology,15:274.
[5]	Chini A, Ben-Romdhane W, Hassairi A, Aboul-Soud M A M. 2017. Identification of TIFY/JAZ family genes in Solanum lycopersicum and their regulation in response to abiotic stresses. PLoS ONE, 12 (6):e0177381. doi: 10.1371/journal.pone.0177381 URL
[6]	Chini A, Fonseca S, Fernández G, Adie B, Chico J M, Lorenzo O, García-Casado G, López-Vidriero I, Lozano F M, Ponce M R, Micol J L, Solano R. 2007. The JAZ family of repressors is the missing link in jasmonate signalling. Nature, 448 (7154):666-671. doi: 10.1038/nature06006
[7]	Creelman R A, Mullet J E. 1997. Biosynthesis and action of jasmonates in plants. Annual Review of Plant Biology,48:355-381.
[8]	Dai Z, Dong S, Miao H, Liu X, Han J, Li C, Gu X, Zhang S. 2022. Genome-Wide identification of TIFY genes and their response to various pathogen infections in cucumber(Cucumis sativus L.). Scientia Horticulturae, 295:110814. doi: 10.1016/j.scienta.2021.110814 URL
[9]	Fu J, Wu H, Ma S, Xiang D, Liu R, Xiong L. 2017. OsJAZ1 attenuates drought resistance by regulating JA and ABA signaling in rice. Frontiers in Plant Science, 8:2108. doi: 10.3389/fpls.2017.02108 pmid: 29312378
[10]	Garcia-Mas J, Benjak A, Sanseverino W, Bourgeois M, Mir G, González V M, Hénaff E, Câmara F, Cozzuto L, Lowy E, Alioto T, Capella-Gutiérrez S, Blanca J, Cañizares J, Ziarsolo P, Gonzalez-Ibeas D, Rodríguez-Moreno L, Droege M, Du L, Alvarez-Tejado M, Lorente-Galdos B, Melé M, Yang L, Weng Y, Navarro A, Marques-Bonet T, Aranda M A, Nuez F, Picó B, Gabaldón T, Roma G, Guigó R, Casacuberta J M, Arús P, Puigdomènech P. 2012. The genome of melon(Cucumis melo L.). Proceedings of National Academy of Sciences of the United States of America, 109 (29):11872-11877.
[11]	Hu S, Yu K, Yan J, Shan X, Xie D. 2023. Jasmonate perception:ligand-receptor interaction,regulation,and evolution. Molecular Plant, 16 (1):23-42. doi: 10.1016/j.molp.2022.08.011 URL
[12]	Hu Y, Jiang L, Wang F, Yu D. 2013. Jasmonate regulates the inducer of CBF expression-C-repeat binding factor/DRE binding factor 1 cascade and freezing tolerance in Arabidopsis. The Plant Cell, 25:2907-2924. doi: 10.1105/tpc.113.112631 URL
[13]	Hu Y, Sun H, Han Z, Wang S, Wang T, Li Q, Tian J, Wang Y, Zhang X, Xu X, Han Z, Wu T. 2022. ERF 4 affects fruit ripening by acting as a JAZ interactor between ethylene and jasmonic acid hormone signaling pathways. Horticultural Plant Journal, 8 (6):689-699. doi: 10.1016/j.hpj.2022.01.002 URL
[14]	Koh H, Joo H, Lim C W, Lee S C. 2023. Roles of the pepper JAZ protein CaJAZ1-03 and its interacting partner RING-type E3 ligase CaASRF1 in regulating ABA signaling and drought responses. Plant Cell Environment, 46 (11):3242-3257. doi: 10.1111/pce.v46.11 URL
[15]	Li M, Yu G, Cao C, Liu P. 2021. Metabolism,signaling,and transport of jasmonates. Plant Communication, 2 (5):100231. doi: 10.1016/j.xplc.2021.100231 URL
[16]	Li Meng, Lü Tinghui, Xing Qiaojuan, Qi Hongyan. 2018. Research progress on evaluation and regulation of chilling tolerance in cucurbitaceous vegetables. Acta Horticulturae Sinica, 45 (9):1761-1777. (in Chinese) doi: 10.16420/j.issn.0513-353x.2018-0028
	李猛, 吕亭辉, 邢巧娟, 齐红岩. 2018. 瓜类蔬菜耐低温性评价与调控研究进展. 园艺学报, 45 (9):1761-1777. doi: 10.16420/j.issn.0513-353x.2018-0028
[17]	Li Qiong, Li Lili, Hou Juan, Luo Renren, Wang Ruidan, Hu Jianbin, Huang Song. 2022. Advances on mechanism of cucurbit crops in response to low-temperature stress. Acta Horticulturae Sinica, 49 (6):1382-1394. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0575
	李琼, 李丽丽, 侯娟, 罗忍忍, 王瑞丹, 胡建斌, 黄松. 2022. 瓜类作物响应低温胁迫机制的研究进展. 园艺学报, 49 (6):1382-1394. doi: 10.16420/j.issn.0513-353x.2021-0575
[18]	Li S, Dong Y, Li D, Shi S, Zhao N, Liao J, Liu Y, Chen H. 2024. Eggplant transcription factor SmMYB5 integrates jasmonate and light signaling during anthocyanin biosynthesis. Plant Physiology, 194 (2):1139-1165. doi: 10.1093/plphys/kiad531 URL
[19]	Li Xiao, Yang Qinrong, Xia Yuelin, Wang Chenhao, Jin Rongrong, Cao Hong, Bo Yongming, Zhang Mingfang, Lü Xiaolong. 2025. Progress in functional gene research on important traits of melons. Acta Horticulturae Sinica, 52 (8):2208-2232. (in Chinese) doi: 10.16420/j.issn.0513-353x.2024-0936
	李潇, 杨沁蓉, 夏玥琳, 王陈昊, 金荣荣, 曹虹, 薄永明, 张明方, 吕小龙. 2025. 甜瓜重要性状功能基因研究进展. 园艺学报, 52 (8):2208-2232. doi: 10.16420/j.issn.0513-353x.2024-0936
[20]	Li Y, Zhang Q, Wang L, Wang X, Qiao J, Wang H. 2023. New insights into the TIFY gene family of Brassica napus and its involvement in the regulation of shoot branching. International Journal of Molecular Science, 24 (23):17114. doi: 10.3390/ijms242317114 URL
[21]	Ling J, Xie X, Gu X, Zhao J, Ping X, Li Y, Yang Y, Mao Z, Xie B. 2021. High-quality chromosome-level genomes of Cucumis metuliferus and Cucumis melo provide insight into Cucumis genome evolution. The Plant Journal, 107 (1):136-148. doi: 10.1111/tpj.v107.1 URL
[22]	Liu Tongjin, Liu Zhenyang, Ban Qiuyan, Zhou Lu, Cui Qunxiang. 2024. Cloning and tissue expression analysis of TIFY genes in Luffa cylindrica. Acta Botanica Boreali-Occidentalia Sinica, 44 (11):1752-1759. (in Chinese)
	刘同金, 刘振洋, 班秋妍, 周露, 崔群香. 2024. 丝瓜TIFY基因克隆及组织表达分析. 西北植物学报, 44 (11):1752-1759.
[23]	Luo Donglan, Ba Liangjie, Chen Jianye, Lu Wangjin, Shan Wei, Kuang Jianfei. 2017. Characterization and expression analysis of banana MaTIFY1 transcription factor during fruit ripening. Acta Horticulturae Sinica, 44 (1):43-52. (in Chinese) doi: 10.16420/j.issn.0513-353x.2016-0454
	罗冬兰, 巴良杰, 陈建业, 陆旺金, 单伟, 邝健飞. 2017. 香蕉MaTIFY1转录因子特性及其在成熟过程中基因表达分析. 园艺学报, 44 (1):43-52. doi: 10.16420/j.issn.0513-353x.2016-0454
[24]	Lv Y, Yang M, Hu D, Yang Z, Ma S, Li X, Xiong L. 2017. The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression. Plant Physiology,173:1475-1491.
[25]	Mei Chuang, Zhang Xiaoyan, Yan Peng, Ai Shajiang maimaiti, Feng Beibei, Ma Kai, Han Liqun, Dong Lianxin, Wang Jixun. 2021. Identification of TIFY family in apple and their expression analysis under insect stress. Acta Horticulturae Sinica, 48 (2):233-242. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0350
	梅闯, 张小燕, 闫鹏, 艾沙江 · 买买提, 冯贝贝, 马凯, 韩立群, 董连新, 王继勋. 2021. 苹果TIFY家族基因鉴定及其在虫害胁迫下的表达分析. 园艺学报, 48 (2):233-242. doi: 10.16420/j.issn.0513-353x.2020-0350
[26]	Nguyen T H, Goossens A, Lacchini E. 2022. Jasmonate:a hormone of primary importance for plant metabolism. Current Opinion in Plant Biology, 67:102197. doi: 10.1016/j.pbi.2022.102197 URL
[27]	Nishii A, Takemura M, Fujita H, Shikata M, Yokota A, Kohchi T. 2000. Characterization of a novel gene encoding a putative single zinc-finger protein,ZIM,expressed during the reproductive phase in Arabidopsis thaliana. Bioscience Biotechnology Biochemistry, 64 (7):1402-1409. doi: 10.1271/bbb.64.1402 URL
[28]	Pauwels L, Barbero G F, Geerinck J, Tilleman S, Grunewald W, Pérez A C, Chico J M, Bossche R V, Sewell J, Gil E, García-Casado G, Witters E, Inzé D, Long J A, De Jaeger G, Solano R, Goossens A. 2010. NINJA connects the co-repressor TOPLESS to jasmonate signalling. Nature, 464 (7289):788-791. doi: 10.1038/nature08854
[29]	Pichot C, Djari A, Tran J, Verdenaud M, Marande W, Huneau C, Gautier V, Latrasse D, Arribat S, Sommard V, Troadec C, Poncet C, Bendahmane M, Szecsi J, Dogimont C, Salse J, Benhamed M, Zouine M, Boualem A, Bendahmane A. 2021. Cantaloupe melon genome reveals 3D chromatin features and structural relationship with the ancestral cucurbitaceae karyotype. iScience, 25 (1):103696. doi: 10.1016/j.isci.2021.103696 URL
[30]	Sheard L B, Tan X, Mao H, Withers J, Ben-Nissan G, Hinds T R, Kobayashi Y, Hsu FF, Sharon M, Browse J, He S Y, Rizo J, Howe G A, Zheng N. 2010. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature, 468 (7322):400-405. doi: 10.1038/nature09430
[31]	Shi Y, Ding Y, Yang S. 2018. Molecular regulation of CBF signaling in cold acclimation. Trends in Plant Science, 23 (7):623-637. doi: S1360-1385(18)30086-4 pmid: 29735429
[32]	Sun Lingmin, Sun Rui, Dong Jing, Zhong Chuanfei, Zhang Yuntao, Sun Jian, Chang Linlin, Wang Guixia, Gao Yongshu, Wei Lingzhi. 2023. Identification and expression pattern analysis of FvTIFY gene family in Fragaria vesca Molecular Plant Breeding, https://link.cnki.net/urlid/46.1068.S.20231218.1133.004. (in Chinese)
	孙灵敏, 孙瑞, 董静, 钟传飞, 张运涛, 孙健, 常琳琳, 王桂霞, 高用顺, 魏灵芝. 2023. 森林草莓FvTIFY基因家族鉴定及其表达模式分析. 分子植物育种, https://link.cnki.net/urlid/46.1068.S.20231218.1133.004.
[33]	Sun P, Shi Y, Valerio A G O, Borrego E J, Luo Q, Qin J, Liu K, Yan Y. 2021. An updated census of the maize TIFY family. PLoS ONE, 16 (2):e0247271. doi: 10.1371/journal.pone.0247271 URL
[34]	Sun Yutong, Liu Deshuai, Huang Xuzheng, Chi Jingnan, Qi Xun, Yao Wenkong. 2024. Study on the cold resistance function of VaJAZ9 and VaCOI1genes in Vitis amurensis. Acta Horticulturae Sinica, 51 (10):2243-2254. (in Chinese)
	孙雨桐, 刘德帅, 黄栩筝, 迟敬楠, 齐迅, 姚文孔. 2024. 山葡萄VaJAZ9 和VaCOI1 基因的抗寒功能研究. 园艺学报, 51 (10):2243-2254.
[35]	Tan Xiaoyu, Niu Junpeng, Wang Quanzhi, Zhang Li, Zhu Lijuan, Wang Guodong, Zheng Heyun, Yu Zhifang, Duan Yuquan, Jiang Li, Sun Xiuxiu, Yang Ying, Luo Weiqi, Li Xuehui, Guan Le, Zhao Yanling, Li Guoxiao, Yin Congfei, Ge Cheng, Ma Min, Jia Luting, Zhang Xu, Zhao Yaoyao, Geng Xinli, Wang Libin, Zhang Shaoling. 2025. Recent advances in jas-induced improvement of postharvest chilling resistance in horticultural crops. Acta Horticulturae Sinica, 52 (9):1987-2020. (in Chinese)
	谭筱玉, 牛军鹏, 王全智, 张丽, 朱丽娟, 王国栋, 郑贺云, 郁志芳, 段玉权, 姜丽, 孙秀秀, 杨瑛, 罗伟奇, 李雪晖, 管乐, 赵艳岭, 李国晓, 殷从飞, 葛成, 马敏, 贾璐婷, 张旭, 赵垚垚, 耿新丽, 王利斌, 张绍铃. 2025. 茉莉酸及其衍生物提高园艺作物采后低温抗性的研究进展. 园艺学报, 52 (8):1987-2020. doi: 10.16420/j.issn.0513-353x.2024-0969
[36]	Tao J, Jia H, Wu M, Zhong W, Jia D, Wang Z, Huang C. 2022. Genome-wide identification and characterization of the TIFY gene family in kiwifruit. BMC Genomics, 23 (1):179. doi: 10.1186/s12864-022-08398-8 pmid: 35247966
[37]	Thines B, Katsir L, Melotto M., Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J. 2007. JAZ repressor proteins are targets of the SCF(COI1)complex during jasmonate signalling. Nature,448:661-665.
[38]	Vanholme B, Grunewald W, Bateman A, Kohchi T, Gheysen G. 2007. The tify family previously known as ZIM. Trends in Plant Science, 12 (6):239-244. doi: 10.1016/j.tplants.2007.04.004 pmid: 17499004
[39]	Wang Lili, Deng Hong, Yu Runrun, Qiao Guang. 2024. Genome-wide identification and expression analysis of sweet cherry TIFY gene family in fruit development. Molecular Plant Breeding, https://link.cnki.net/urlid/46.1068.S.20240115.1507.006. (in Chinese)
	王丽丽, 邓鸿, 于润润, 乔光. 2024. 甜樱桃TIFY基因家族全基因组鉴定及在果实发育中的表达分析. 分子植物育种, https://link.cnki.net/urlid/46.1068.S.20240115.1507.006.
[40]	Wang W, Ouyang J, Li Y, Zhai C, He B, Si H, Chen K, Rose J K C, Jia W. 2024. A signaling cascade mediating fruit trait development via phosphorylation-modulated nuclear accumulation of JAZ repressor. Journal of Integrative Plant Biology, 66 (6):1106-1125. doi: 10.1111/jipb.13654
[41]	Wasternack C, Hause B. 2013. Jasmonates:biosynthesis,perception,signal transduction and action in plant stress response,growth and development. An update to the 2007 review in Annals of Botany. Annals of Botany, 111 (6):1021-1058. doi: 10.1093/aob/mct067 pmid: 23558912
[42]	White D W. 2006. PEAPOD regulates lamina size and curvature in Arabidopsis. Proceedings of National Academy of Sciences of the United States of America, 103 (35):13238-13243.
[43]	Xie D X, Feys B F, James S, Nieto-Rostro M, Turner J G. 1998. COI1:an Arabidopsis gene required for jasmonate-regulated defense and fertility. Science, 280 (5366):1091-1904. doi: 10.1126/science.280.5366.1091 pmid: 9582125
[44]	Xie Siyi, Zhou Chengzhe, Zhu Chen, Zhan Dongmei, Chen Lan, Wu Zuchun, Lai Zhongxiong, Guo Yuqiong. 2022. Genome-wide identification and expression analysis of CsTIFY transcription factor family under abiotic stress and hormone treatments in Camellia sinensis. Acta Horticulturae Sinica, 49 (1):100-116. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0850
	谢思艺, 周承哲, 朱晨, 詹冬梅, 陈兰, 吴祖春, 赖钟雄, 郭玉琼. 2022. 茶树CsTIFY家族全基因组鉴定及非生物胁迫和激素处理中主要基因表达分析. 园艺学报, 49 (1):100-116. doi: 10.16420/j.issn.0513-353x.2020-0850
[45]	Xu L, Liu F, Lechner E, Genschik P, Crosby W L, Ma H, Peng W, Huang D, Xie D. 2002. The SCF(COI1)ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis. The Plant Cell, 14 (8):1919-1935. doi: 10.1105/tpc.003368 URL
[46]	Yan J, Zhang C, Gu M, Bai Z, Zhang W, Qi T, Cheng Z, Peng W, Luo H, Nan F, Wang Z, Xie D. 2009. The Arabidopsis CORONATINE INSENSITIVE 1 protein is a jasmonate receptor. The Plant Cell, 21 (8):2220-2236. doi: 10.1105/tpc.109.065730 URL
[47]	Yang Y, Ahammed G J, Wan C, Liu H, Chen R, Zhou Y. 2019. Comprehensive analysis of TIFY transcription factors and their expression profiles under jasmonic acid and abiotic stresses in watermelon. International Journal of Genomics, 2019:6813086.
[48]	Yu J, Zhang Y, Di C, Zhang Q, Zhang K, Wang C, You Q, Yan H, Dai S Y, Yuan J S, Xu W, Su Z. 2016. JAZ7 negatively regulates dark-induced leaf senescence in Arabidopsis. Journal of Experimental Botany, 67 (3):751-762. doi: 10.1093/jxb/erv487 URL
[49]	Yu X, Chen G, Tang B, Zhang J, Zhou S, Hu Z. 2018. The jasmonate ZIM-domain protein gene SlJAZ 2 regulates plant morphology and accelerates flower initiation in Solanum lycopersicum plants. Plant Science,267:65-73.
[50]	Yuan Xiao, Jiang Yuanyuan, Zhu Yunna, Qu Shanshan, Wang Yukun, Yuan Yuan, Wang Bin. 2024. Expression analysis of JAZ family genes in harvested cucumber fruit under cold storage condition. Acta Agriculturae Zhejiangensis, 36 (8):1820-1831. (in Chinese) doi: 10.3969/j.issn.1004-1524.20231044
	袁晓, 蒋园园, 朱云娜, 曲姗姗, 王玉昆, 原远, 王斌. 2024. JAZ家族基因在采后黄瓜低温贮藏条件下的表达分析. 浙江农业学报, 36 (8):1820-1831. doi: 10.3969/j.issn.1004-1524.20231044
[51]	Zhai Q, Zhang X, Wu F, Feng H, Deng L, Xu L, Zhang M, Wang Q, Li C. 2015. Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis. The Plant Cell, 27 (10):2814-2828.
[52]	Zhang H, Li X, Yu H, Zhang Y, Li M, Wang H, Wang D, Wang H, Fu Q, Liu M, Ji C, Ma L, Tang J, Li S, Miao J, Zheng H, Yi H. 2019. A high-quality melon genome assembly provides insights into genetic basis of fruit trait improvement. iScience,22:16-27.
[53]	Zhang J, Liu R, Zhang S, Ge C, Liu S, Ma H, Pang C, Shen Q. 2024. Integrating physiological and transcriptomic analyses explored the regulatory mechanism of cold tolerance at seedling emergence stage in upland cotton(Gossypium hirsutum L.). Plant Physiology and Biochemistry, 217:109297. doi: 10.1016/j.plaphy.2024.109297 URL
[54]	Zhao G, Lian Q, Zhang Z, Fu Q, He Y, Ma S, Ruggieri V, Monforte A, Wang P, Julca I, Wang H, Liu J, Xu Y, Wang R, Ji J, Xu Z, Kong W, Zhong Y, Shang J, Pereira L, Argyris J, Zhang J, Mayobre C, Pujol M, Oren E, Ou D, Wang J, Sun D, Zhao S, Zhu Y, Li N, Katzir N, Gur A, Dogimont C, Schaefer H, Fan W, Bendahmane A, Fei Z, Pitrat M, Gabaldón T, Lin T, Garcia-Mas J, Xu Y, Huang S. 2019. A comprehensive genome variation map of melon identifies multiple domestication events and loci influencing agronomic traits. Nature Genetics, 51 (11):1607-1615. doi: 10.1038/s41588-019-0522-8 pmid: 31676864
[55]	Zhao X, He Y, Liu Y, Wang Z, Zhao J. 2024. JAZ proteins:key regulators of plant growth and stress response. The Crop Journal, 12 (6):1505-1516. doi: 10.1016/j.cj.2024.11.001 URL

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CmTIFY1	CAGCAAAGCGTGGATTTGGTA	TCGAAACCGATTTAACGAGGC
CmTIFY4	TGCAGACTTCCAGTGACAGAG	CGACAATCCAGAATCCCGCA
CmTIFY5A	ACGTCGAGGAGAGATCGACA	TAGAGAGCCCAGAACCGGAA
CmTIFY5B	CCACGCCTGATCTTACCGAG	CCCGCTCCTCATCCATACTC
CmTIFY5C	TGAACTTCAGGCAAGAGCCAT	ATGGAGAAACCTGCGTTCCC
CmTIFY5D	GGCAACCAGAGAAATGGAGGA	TTTCTTCATCGAAAGCCCCG
CmTIFY6A	ATGTTCTTAGCTGGGGCTGG	ATAGGAGCAGCATCACTTGC
CmTIFY6B	TTGCAACCTCCCTTTCGGTT	CACACGGATCCGGCATAGAA
CmTIFY8A	AGCCACCACGAATTGCTTCT	ACACGAGGCACAAACTGACT
CmTIFY8B	GAAAGCTGTGTGTTGCTGGG	CTTACCACCACTGCTGCCAT
CmTIFY9A	GTTCTCTCGCCTTTGCCTCT	GAACGTCAAAAACGGCGACA
CmTIFY9B	CGCCTCTGAATCCAGGTTGA	TGCTTTTGGCCTCATCACGA
CmTIFY10	ACGCTGGCCAAGTTTTTGTG	GAGTTCCGACCAAGATGGGG
CmTIFY11A	AGAAATCTGCCTCTGTGGCG	CCCTCTCCGACGGTAGATCA
CmTIFY11B	CGCCGGAAAAGTTTTGGTGT	ATAATCTGTTGGGGGCGACG
CmActin	GCCCTTCCTCATGCCATTCT	CAATTTCCCGTTCGGCAGTG

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
CmTIFY1	CAGCAAAGCGTGGATTTGGTA	TCGAAACCGATTTAACGAGGC
CmTIFY4	TGCAGACTTCCAGTGACAGAG	CGACAATCCAGAATCCCGCA
CmTIFY5A	ACGTCGAGGAGAGATCGACA	TAGAGAGCCCAGAACCGGAA
CmTIFY5B	CCACGCCTGATCTTACCGAG	CCCGCTCCTCATCCATACTC
CmTIFY5C	TGAACTTCAGGCAAGAGCCAT	ATGGAGAAACCTGCGTTCCC
CmTIFY5D	GGCAACCAGAGAAATGGAGGA	TTTCTTCATCGAAAGCCCCG
CmTIFY6A	ATGTTCTTAGCTGGGGCTGG	ATAGGAGCAGCATCACTTGC
CmTIFY6B	TTGCAACCTCCCTTTCGGTT	CACACGGATCCGGCATAGAA
CmTIFY8A	AGCCACCACGAATTGCTTCT	ACACGAGGCACAAACTGACT
CmTIFY8B	GAAAGCTGTGTGTTGCTGGG	CTTACCACCACTGCTGCCAT
CmTIFY9A	GTTCTCTCGCCTTTGCCTCT	GAACGTCAAAAACGGCGACA
CmTIFY9B	CGCCTCTGAATCCAGGTTGA	TGCTTTTGGCCTCATCACGA
CmTIFY10	ACGCTGGCCAAGTTTTTGTG	GAGTTCCGACCAAGATGGGG
CmTIFY11A	AGAAATCTGCCTCTGTGGCG	CCCTCTCCGACGGTAGATCA
CmTIFY11B	CGCCGGAAAAGTTTTGGTGT	ATAATCTGTTGGGGGCGACG
CmActin	GCCCTTCCTCATGCCATTCT	CAATTTCCCGTTCGGCAGTG

基因 Gene	基因序列号 Gene ID	CDS长度/bp CDS length	氨基酸数 Number of amino acids	相对分子质量/kD Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
CmTIFY1	MELO3C011079	882	293	31.69	6.60	细胞核Nucleus
CmTIFY4	MELO3C014283	921	306	33.33	5.39	细胞核Nucleus
CmTIFY5A	MELO3C001202	450	149	16.83	9.02	细胞核Nucleus
CmTIFY5B	MELO3C002741	363	120	13.44	10.03	细胞核Nucleus
CmTIFY5C	MELO3C016195	384	127	14.84	10.88	细胞核Nucleus
CmTIFY5D	MELO3C007125	399	132	14.67	10.23	细胞核Nucleus
CmTIFY6A	MELO3C015030	1 062	353	36.71	8.66	细胞核Nucleus
CmTIFY6B	MELO3C002143	1 032	343	36.83	9.12	细胞核Nucleus
CmTIFY8A	MELO3C003675	1 215	404	43.32	8.61	细胞核Nucleus
CmTIFY8B	MELO3C011217	1 362	453	47.69	9.05	细胞核Nucleus
CmTIFY9A	MELO3C022348	558	185	20.35	9.64	细胞核Nucleus
CmTIFY9B	MELO3C017797	603	200	22.23	7.95	细胞核Nucleus
CmTIFY10	MELO3C015538	690	229	24.61	10.06	细胞核Nucleus
CmTIFY11A	MELO3C022678	630	209	22.73	9.32	细胞核Nucleus
CmTIFY11B	MELO3C024336	903	300	32.46	9.38	细胞核Nucleus

基因 Gene	基因序列号 Gene ID	CDS长度/bp CDS length	氨基酸数 Number of amino acids	相对分子质量/kD Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
CmTIFY1	MELO3C011079	882	293	31.69	6.60	细胞核Nucleus
CmTIFY4	MELO3C014283	921	306	33.33	5.39	细胞核Nucleus
CmTIFY5A	MELO3C001202	450	149	16.83	9.02	细胞核Nucleus
CmTIFY5B	MELO3C002741	363	120	13.44	10.03	细胞核Nucleus
CmTIFY5C	MELO3C016195	384	127	14.84	10.88	细胞核Nucleus
CmTIFY5D	MELO3C007125	399	132	14.67	10.23	细胞核Nucleus
CmTIFY6A	MELO3C015030	1 062	353	36.71	8.66	细胞核Nucleus
CmTIFY6B	MELO3C002143	1 032	343	36.83	9.12	细胞核Nucleus
CmTIFY8A	MELO3C003675	1 215	404	43.32	8.61	细胞核Nucleus
CmTIFY8B	MELO3C011217	1 362	453	47.69	9.05	细胞核Nucleus
CmTIFY9A	MELO3C022348	558	185	20.35	9.64	细胞核Nucleus
CmTIFY9B	MELO3C017797	603	200	22.23	7.95	细胞核Nucleus
CmTIFY10	MELO3C015538	690	229	24.61	10.06	细胞核Nucleus
CmTIFY11A	MELO3C022678	630	209	22.73	9.32	细胞核Nucleus
CmTIFY11B	MELO3C024336	903	300	32.46	9.38	细胞核Nucleus

Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon

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