SlMAPKKK43 Regulates Tomato Resistance to Gray Mold

doi:10.16420/j.issn.0513-353x.2023-0757

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (2): 309-320.doi: 10.16420/j.issn.0513-353x.2023-0757

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

SlMAPKKK43 Regulates Tomato Resistance to Gray Mold

DONG Xiaonan1，LÜ Hongmei1，ZHAO Liqun3，HE Bingqing4，ZHANG Jiaojiao1，ZHAO Bing1，GUO Yangdong1,2,*，and ZHANG Na1,2,*

1Beijing Key Laboratory of Growth and Development Regulation of Protected Vegetables，Department of Vegetable，College of Horticulture，China Agricultural University，Beijing 100193，China；2Sanya Institute of China Agricultural University，Sanya，Hainan 572025，China；3Beijing Agricultural Technology Extension Station，Beijing 100029，China；4Beijing Changping District Agricultural Technology Extension Station，Beijing 102200，China

Online:2024-02-25 Published:2024-02-26

Abstract

Abstract: The mitogen-activated protein kinase（MAPK）cascade plays an important role in multiple developmental and physiological processes in plants and in response to various biotic and abiotic stresses. In this study，SlMAPKKK43 overexpressed plants and CRISPR-Cas9-mediated SlMAPKKK43 knockout mutant using Agrobacterium infection genetic transformation were obtained. Through the inoculation of Botrytis cinerea with transgenic lines，we found that SlMAPKKK43 positively regulated the tolerance of tomato to Botrytis cinerea. In order to further elucidate the molecular mechanism of SlMAPKKK43 regulating the resistance of gray mold，the known gray mold regulators SlMKK2 and SlMKK4 were screened by pull down-MS technology，and in vitro phosphorylation assay was used to preliminarily verify that SlMAPKKK43 could phosphorylate SlMKK2 and SlMKK4.

Key words: tomato, Botrytis cinerea, MAPK cascade, SlMAPKKK43

CLC Number:

S 641.2

DONG Xiaonan, LÜ Hongmei, ZHAO Liqun, HE Bingqing, ZHANG Jiaojiao, ZHAO Bing, GUO Yangdong, and ZHANG Na, . SlMAPKKK43 Regulates Tomato Resistance to Gray Mold[J]. Acta Horticulturae Sinica, 2024, 51(2): 309-320.

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SlMAPKKK43 Regulates Tomato Resistance to Gray Mold

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