The Sugar Transporter SlSWEET12c Negatively Regulates Tomato Resistance to Bacterial Leaf Spot Disease

doi:10.16420/j.issn.0513-353x.2023-0611

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (2): 321-334.doi: 10.16420/j.issn.0513-353x.2023-0611

• Genetic&Breeding?Germplasm Resources?Molecular Biology • Previous Articles Next Articles

The Sugar Transporter SlSWEET12c Negatively Regulates Tomato Resistance to Bacterial Leaf Spot Disease

XING Yanan¹, CHENG Jie¹, LIU Xin¹, LÜ Shuwen², WU Yuanyuan²^,^*(), JIANG Jing¹^,^*()

¹ College of Horticulture，Shenyang Agricultural University，Shenyang 110866，China
² Institute of Vegetables，Liaoning Academy of Agricultural Sciences，Shenyang 110161，China

Received:2023-09-20 Revised:2024-01-04 Online:2024-02-25 Published:2024-02-26
Contact: WU Yuanyuan, JIANG Jing

Abstract

Abstract:

According to the tomato expression database（http://ted.bti.cornell.edu/cgi-bin/）and real-time quantitative analysis，the sugar transporter（sugars will eventually be exported transporter）gene SlSWEET12c showed the most obvious expression difference in tomato susceptible and resistant varieties. Using the tomato（Solanum lycopersicum）plant‘Zhongshu 6’as the test material，stable genetic SlSWEET12c silencing（RNAi）and overexpression（OE）plants were obtained. After further inoculation with Pseudomonas syringae（Pst DC3000），the role of SWEET12c in resisting Pst DC3000 in tomato was clarified through the analysis of bacterial growth，F_v/F_m change，soluble sugar and starch content，reactive oxygen species accumulation，defense enzyme activity and PR protein related gene expression in leaves. Compared with the wild type，the number of bacterial colonies and brown spots were significantly reduced in the leaves of RNAi-SlSWEET12c plants vaccinated with Pst DC3000，and the resistance to Pst DC3000 was enhanced，while OE-SlSWEET12c leaves became more severe，and the number of bacterial colonies and brown spots increased. RNAi-SlSWEET12c plants have significantly higher sucrose and starch content than wild type，reactive oxygen accumulation is less than wild type，the activity of defense enzymes （POD，SOD，APX）and PR protein related genes（NPR1，PR1，PR2，PR5）expression，while the sucrose and starch content of OE-SlSWEET12c plants is lower than wild type，reactive oxygen accumulation is higher than wild type，defense enzyme activity and PR protein related gene expression is significantly lower than wild type. The above results indicate that SlSWEET12c negatively regulates tomato resistance to bacterial leaf spot disease by affecting the composition of sugars in leaves.

Key words: tomato, sugar transporter, bacterial leaf spot disease, SlSWEET12c

XING Yanan, CHENG Jie, LIU Xin, LÜ Shuwen, WU Yuanyuan, JIANG Jing. The Sugar Transporter SlSWEET12c Negatively Regulates Tomato Resistance to Bacterial Leaf Spot Disease[J]. Acta Horticulturae Sinica, 2024, 51(2): 321-334.

Figures/Tables 10

References 49

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基因编号 Gene accession	基因名称 Gene name	正向引物（5′-3′） Forword primer	逆向引物（5′-3′） Reverse primer
Solyc03g097580	SlSWEET10a	TGGTTGGTGGATTTGGTGC	TTGTCTCACGATGCCTAAGGG
Solyc03g097600	SlSWEET10b	TCCTTTCTCCATTGCCAACG	GCACCAAATCCACCCAC
Solyc03g097610	SlSWEET10c	GATGGCTATTGCTGGTCATTGG	ACCCTGGCTTTCTTTGGTGC
Solyc03g097870	SlSWEET11a	CTACGCACCAAAGAAAGCCAG	GTCTCACAATGCCTAAGGGAGC
Solyc03g097570	SlSWEET11b	CCAACACAACACTTCTCATCAC	GTCCACGAACAACTCCTTTG
Solyc06g072620	SlSWEET11c	GCACCAAAGAAAGCCAGGGT	CACAAGGCAAATCCATCCAAC
Solyc06g072640	SlSWEET11d	TCTCGTTCATTGTGTTCCTTGC	TGGACCTTGGCTTTCTTTGG
Solyc03g097590	SlSWEET12a	ACCAAACAAAGCCAGGGTCC	TCTCACAATGCCTAAGGGTGC
Solyc03g097620	SlSWEET12b	TGGTCTACACCTTTGGCATT	CCCTTCCGATGATTTCTTCT
Solyc05g024260	SlSWEET12c	GCATCGTGTTTCAAGTGGTTCG	TCTATCGCTGGCTTTGCGTT
Solyc06g072630	SlSWEET12d	AAGCCAGGGTCCAAACTGT	GGAATCGCAATGTTGAAGTC
Solyc03g097560	SlSWEET14	GCCAAAGGCAATCATAGAGG	AGGCACACACAATCAGACCT
Solyc09g074530	SlSWEET15	TACGCCACCAAATCGGCTAA	TGGCATGAACTCAACGCTCT
Solyc07g040690.2	SlNPR1	GGGAAAGATAGCAGCACG	GTCCACACAAACACACACATC
NC_015444.3	SlPR1	CCGTGCAATTGTGGGTGTC	GAGTTGCGCCAGACTACTTGAGT
M80604	SlPR2	TCCAGGTAGAGACAGTGGTAAA	CCTAAATATGTCGCGGTTGAGA
NM_001330783	SlPR5	TCCTTGCCTTTGTGACTT	ACCTCTTCCAGCACCATC

基因编号 Gene accession	基因名称 Gene name	正向引物（5′-3′） Forword primer	逆向引物（5′-3′） Reverse primer
Solyc03g097580	SlSWEET10a	TGGTTGGTGGATTTGGTGC	TTGTCTCACGATGCCTAAGGG
Solyc03g097600	SlSWEET10b	TCCTTTCTCCATTGCCAACG	GCACCAAATCCACCCAC
Solyc03g097610	SlSWEET10c	GATGGCTATTGCTGGTCATTGG	ACCCTGGCTTTCTTTGGTGC
Solyc03g097870	SlSWEET11a	CTACGCACCAAAGAAAGCCAG	GTCTCACAATGCCTAAGGGAGC
Solyc03g097570	SlSWEET11b	CCAACACAACACTTCTCATCAC	GTCCACGAACAACTCCTTTG
Solyc06g072620	SlSWEET11c	GCACCAAAGAAAGCCAGGGT	CACAAGGCAAATCCATCCAAC
Solyc06g072640	SlSWEET11d	TCTCGTTCATTGTGTTCCTTGC	TGGACCTTGGCTTTCTTTGG
Solyc03g097590	SlSWEET12a	ACCAAACAAAGCCAGGGTCC	TCTCACAATGCCTAAGGGTGC
Solyc03g097620	SlSWEET12b	TGGTCTACACCTTTGGCATT	CCCTTCCGATGATTTCTTCT
Solyc05g024260	SlSWEET12c	GCATCGTGTTTCAAGTGGTTCG	TCTATCGCTGGCTTTGCGTT
Solyc06g072630	SlSWEET12d	AAGCCAGGGTCCAAACTGT	GGAATCGCAATGTTGAAGTC
Solyc03g097560	SlSWEET14	GCCAAAGGCAATCATAGAGG	AGGCACACACAATCAGACCT
Solyc09g074530	SlSWEET15	TACGCCACCAAATCGGCTAA	TGGCATGAACTCAACGCTCT
Solyc07g040690.2	SlNPR1	GGGAAAGATAGCAGCACG	GTCCACACAAACACACACATC
NC_015444.3	SlPR1	CCGTGCAATTGTGGGTGTC	GAGTTGCGCCAGACTACTTGAGT
M80604	SlPR2	TCCAGGTAGAGACAGTGGTAAA	CCTAAATATGTCGCGGTTGAGA
NM_001330783	SlPR5	TCCTTGCCTTTGTGACTT	ACCTCTTCCAGCACCATC

基因名 Gene name	4 h			6 h
基因名 Gene name	抗病材料 Rio Grande-PtoR （RG-PtoR）	感病材料 Rio Grande- prf 3 （RG-prf 3）	感病材料 Rio Grande-prf 19 （RG-prf 19）	抗病材料 Rio Grande-PtoR （RG-PtoR）	感病材料 Rio Grande- prf 3 （RG-prf 3）		感病材料 Rio Grande-prf 19 （RG-prf 19）
SlSWEET10a	6.90	23.49	20.01	2.86	22.47	30.31
SlSWEET10b	8.57	8.28	9.76	8.78	21.75	25.33
SlSWEET10c	5.78	24.50	28.60	7.88	46.53	50.78
SlSWEET11a	142.08	446.79	431.44	103.71	503.59	470.25
SlSWEET11b	6.24	3.78	2.33	3.47	4.50	4.83
SlSWEET11c	0	0.77	1.87	0	4.45	5.33
SlSWEET11d	0	0	0	0	0	0.12
SlSWEET12a	13.01	67.18	60.92	4.98	67.74	74.78
SlSWEET12b	0	0	0	0	0	0
SlSWEET12c	4.99	80.27	66.06	1.22	83.32	57.78
SlSWEET12d	0.12	3.59	2.87	0.12	5.96	5.85
SlSWEET14	0.02	0.02	0	0.02	0	0
SlSWEET15	0	0	0	0	0	0

基因名 Gene name	4 h			6 h
基因名 Gene name	抗病材料 Rio Grande-PtoR （RG-PtoR）	感病材料 Rio Grande- prf 3 （RG-prf 3）	感病材料 Rio Grande-prf 19 （RG-prf 19）	抗病材料 Rio Grande-PtoR （RG-PtoR）	感病材料 Rio Grande- prf 3 （RG-prf 3）		感病材料 Rio Grande-prf 19 （RG-prf 19）
SlSWEET10a	6.90	23.49	20.01	2.86	22.47	30.31
SlSWEET10b	8.57	8.28	9.76	8.78	21.75	25.33
SlSWEET10c	5.78	24.50	28.60	7.88	46.53	50.78
SlSWEET11a	142.08	446.79	431.44	103.71	503.59	470.25
SlSWEET11b	6.24	3.78	2.33	3.47	4.50	4.83
SlSWEET11c	0	0.77	1.87	0	4.45	5.33
SlSWEET11d	0	0	0	0	0	0.12
SlSWEET12a	13.01	67.18	60.92	4.98	67.74	74.78
SlSWEET12b	0	0	0	0	0	0
SlSWEET12c	4.99	80.27	66.06	1.22	83.32	57.78
SlSWEET12d	0.12	3.59	2.87	0.12	5.96	5.85
SlSWEET14	0.02	0.02	0	0.02	0	0
SlSWEET15	0	0	0	0	0	0

The Sugar Transporter SlSWEET12c Negatively Regulates Tomato Resistance to Bacterial Leaf Spot Disease

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