Effects of Brassinolides and Methyl Jasmonate Spraying on the Postharvest Quality of Apple Fruit

doi:10.16420/j.issn.0513-353x.2022-0594

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (10): 2236-2248.doi: 10.16420/j.issn.0513-353x.2022-0594

• Research Papers • Previous Articles Next Articles

Effects of Brassinolides and Methyl Jasmonate Spraying on the Postharvest Quality of Apple Fruit

SUN Jiamao¹, CUI Quanshi¹, WANG Yuqing¹, SI Yajing¹, SHI Yufan¹, BU Haidong², YUAN Hui¹^,^*(), and WANG Aide¹^,^*()

¹Key Laboratory of Fruit Postharvest Biology,State Education Ministry and Liaoning Provincial Key Laboratory of Protected Horticulture,National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology（Liaoning）,College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China
²Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences,Mudanjiang,Heilongjiang 157041,China

Received:2022-07-25 Revised:2022-08-15 Online:2022-10-25 Published:2022-10-31
Contact: YUAN Hui,and WANG Aide E-mail:huiyuan@syau.edu.cn;awang@syau.edu.cn

Abstract

Abstract:

The purpose of this study was to explore the effects of brassinolides（EBR）and methyl jasmonate（MeJA）on apple fruit quality during storage. The'Yuehua'apple fruit was used as a test material at different concentrations of EBR（0,0.02,0.05 mg · L^-1）with MeJA（0,20,50 mg · L^-1） mixed in pairs,and sprayed once on the fruit 30 days before harvest,and distilled water was sprayed as the control. The results showed that the mixed treatment of 20 mg · L^-1 MeJA + 0.05 mg · L^-1 EBR could delay the fruit firmness,titratable acid content,ascorbic acid content and soluble solids content increase of 'Yuehua'fruit during storage at room temperature,and prolong the fruit storage period. At the same time,the production of ethylene was 17.4% lower than that of the control,and the expression of ethylene synthesis genes MdACO1,MdACS1 and ethylene response factor MdERF3 were inhibited. 20 mg · L^-1 MeJA + 0.05 mg · L^-1 EBR treatment increased the color difference and anthocyanin content of the fruit by 7.3 and 1.4 times compared with the control,respectively,and increased the expression of MdMYB1,MdCHS,MdDFR and MdF3H in the peel. Moreover,50 mg · L^-1 MeJA + 0.05 mg · L^-1 EBR treatment group significantly increased the color difference and anthocyanin content of the fruit;however,the effect on other indicators was not significant. In summary,the treatment of 20 mg · L^-1 MeJA + 0.05 mg · L^-1 during apple fruit development had a significant promoting effect on the maintenance of internal quality and the improvement of appearance quality during fruit storage.

Key words: apple, fruit quality, brassinolides, methyl jasmonate, anthocyanidin

CLC Number:

S661.1

SUN Jiamao, CUI Quanshi, WANG Yuqing, SI Yajing, SHI Yufan, BU Haidong, YUAN Hui, and WANG Aide. Effects of Brassinolides and Methyl Jasmonate Spraying on the Postharvest Quality of Apple Fruit[J]. Acta Horticulturae Sinica, 2022, 49(10): 2236-2248.

Figures/Tables 6

Table 1 Primers used in the study

基因名称	引物序列 Primer sequence（5′-3′）
Gene name	正向 Forward	反向 Reverse
MdACO1	GTTCTACAACCCAGGCAACG	TCTCAGAGCTCAGGCAGTTG
MdACS1	ACAGCCTCTCTAAGGATCTTGGTCT	TTTGGTTCTCGGCTATGTAGTTCTT
MdERF3	GCGAAGGGGAAGCATTACAG	TCCCTGCTCCCATTTCCAAT
MdMYB1	AAGACCTCAGCCCCAAAAATTCAA	CTTTTGAATGATTCCAAAGGTCCG
MdCHS	TCAAAACGACCGGGGAGGGA	ACCCAGGTGGCCCTGCTATTCT
MdF3H	GGGATGATGGGAAGACATGGAT	AGTGCCCTCAGCCCGACCTAAC
MdDFR	GTTGAGGGAGATAGGGTTTGAGTTCA	AAATACCCCGAATACAATATACCCAC

Fig. 1 Effects of MeJA and EBR treatments on the exterior quality of the'Yuehua'apple fruit Different lowercase letters indicate significant differences at 0.05 level between treatments over the same period. The same below.

Fig. 2 Effects of MeJA and EBR treatments on the internal quality of the'Yuehua'apple fruit

Fig. 3 Effects of MeJA and EBR treatments on ethylene production of the'Yuehua'apple fruit

Fig. 4 Effects of MeJA and EBR treatments on gene expression associated with ethylene synthesis and response

Fig. 5 Effects of MeJA and EBR treatments on anthocyanin synthesis and regulation of gene expression

References 50

[1]	Allan A C, Hellens R P, Laing W A. 2008. MYB transcription factors that colour our fruit. Trends Plant Sci, 13 (3):99-102. doi: 10.1016/j.tplants.2007.11.012 pmid: 18280199
[2]	An Jian-ping. 2020. Study on the mechanism of hormonal and environmental signals regulating anthocyanin biosynthesis in apple[Ph. D. Dissertation]. Tai'an: Shandong Agricultural University. (in Chinese)
	安建平. 2020. 激素和环境信号调控苹果花青苷生物合成的机理研究[博士论文]. 泰安: 山东农业大学.
[3]	Cao S F, Zheng Y H, Wang K T, Jin P, Rui H J. 2009. Methyl jasmonate reduces chilling injury and enhances antioxidant enzyme activity in postharvest loquat fruit. Food Chemistry, 115 (4):1458-1463. doi: 10.1016/j.foodchem.2009.01.082 URL
[4]	Chai Y M, Zhang Q, Tian L, Li C L, Xing Y, Qin L, Shen Y Y. 2012. Brassinosteroid is involved in strawberry fruit ripening. Plant Growth Regul, 69:63-69. doi: 10.1007/s10725-012-9747-6 URL
[5]	Chen Ping-zhao, Jiang Bin, Wu Wei-hua, Qi Cen, Cai Zhao-xiang, Zhang Hua. 2013. The effect of foliage spraying sodium bisulfite on storage physiology of Red Fuji apple. Hubei Agricultural Sciences, 52 (6):1395-1397. (in Chinese)
	陈屏昭, 蒋彬, 武卫华, 祁岑, 蔡兆翔, 张桦. 2013. 喷施NaHSO₃溶液对红富士苹果贮藏生理的影响. 湖北农业科学, 52 (6):1395-1397.
[6]	Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. 2010. MYB transcription factors in Arabidopsis. Trends Plant Sci, 15 (10):573-581. doi: 10.1016/j.tplants.2010.06.005 URL
[7]	Flores G, Gracia P B, María L R, Del C. 2015. Postharvest treatment with (-) and (+)-methyl jasmonate stimulates anthocyanin accumulation in grape. LWT - Food Science and Technology, 62 (1):807-812. doi: 10.1016/j.lwt.2014.12.033 URL
[8]	Ge Yu-quan, Li Hong-feng, Wang Chun-yan, Wei Xiao-xia, Su Juan, Dong Sha-sha. 2021. Status and sustainable development proposals of apple industry in China. Modern Food,(11):4-6. (in Chinese)
	葛玉全, 李红锋, 王春燕, 魏晓霞, 苏娟, 东莎莎. 2021. 我国苹果产业现状及可持续发展建议. 现代食品,( 11):4-6.
[9]	Gong De-qiang, Gu Hui, Zhang Lu-bin, Hong Ke-qian, Zhu Shi-jiang. 2013. Effects of pretharvest methyl jasmonate spraying on disease resistance and postharvest quality of mango Fruits. Acta Horticulturae Sinica, 40 (1):49-57. (in Chinese)
	弓德强, 谷会, 张鲁斌, 洪克前, 朱世江. 2013. 杧果采前喷施茉莉酸甲酯对其抗病性和采后品质的影响. 园艺学报, 40 (1):49-57.
[10]	He Ping, Li Lin-guang, Wang Hai-bo, Chang Yuan-sheng. 2019. Effects of jasmonates on coloration and quality of Qiutian peach. Molecular Plant Breeding, 17 (7):2371-2378. (in Chinese)
	何平, 李林光, 王海波, 常源升. 2019. 茉莉酸酯类对秋甜桃果实着色及品质的影响. 分子植物育种, 17 (7):2371-2378.
[11]	He Y, Li J, Ban Q, Han S, Rao J. 2018. Role of brassinosteroids in persimmon(Diospyros kaki L.)fruit ripening. J Agric Food Chem, 66:2637-2644. doi: 10.1021/acs.jafc.7b06117 URL
[12]	He Yan, Sun Yan li, Zhao Fang fang, Dai Hong jun. 2022. Effect of exogenous brassinolides treatment on sugar metabolism of merlot grape berries. Acta Horticulturae Sinica, 49 (1):117-128. (in Chinese)
	贺琰, 孙艳丽, 赵芳芳, 代红军. 2022. 外源油菜素内酯处理对'美乐'葡萄果实糖代谢的影响. 园艺学报, 49 (1):117-128.
[13]	Honda C, Moriya S. 2018. Anthocyanin biosynthesis in apple fruit. Horticulture Journal, 87 (3):305-314. doi: 10.2503/hortj.OKD-R01 URL
[14]	Hong Yanhong, Ye Qinghua, Li Zekun, Wang Wei, Xie Qian, Chen Qingxi, Chen Jianqing. 2021. Accumulation of anthocyanins in red-flowered strawberry'Meihong'petals and expression analysis of MYB gene. Acta Horticulturae Sinica, 48 (8):1470-1484. (in Chinese)
	洪燕红, 叶清华, 李泽坤, 王威, 谢倩, 陈清西, 陈建清. 2021. 红花草莓'莓红'花瓣花色苷积累及其MYB基因的表达分析. 园艺学报, 48 (8):1470-1484.
[15]	Jaakola L. 2013. New insights into the regulation of anthocyanin biosynthesis in fruits. Trends Plant Sci, 18 (9):477-483. doi: 10.1016/j.tplants.2013.06.003 pmid: 23870661
[16]	Ji Y L, Qu Y, Jiang J J, Yan Z Y, Chu J F, Xu M Y, Su X, Yuan H, Wang A D. 2021. The mechanism for brassinosteroids suppressing climacteric fruit ripening. Plant Physiology, 185 (4):1875-1893. doi: 10.1093/plphys/kiab013 pmid: 33743010
[17]	Ke Yujie, Chen Mingkun, Ma Shanhu, Ou Yue, Wang Yi, Zheng Qingdong, Liu Zhongjian, Ai Ye. 2021. Research progress of MYB transcription factors in Orchidaceae. Acta Horticulturae Sinica, 48 (11):2311-2320. (in Chinese)
	柯玉洁, 陈明堃, 马山虎, 欧悦, 王艺, 郑清冬, 刘仲健, 艾叶. 2021. 兰科植物MYB转录因子研究进展. 园艺学报, 48 (11):2311-2320.
[18]	Kucuker E, Oztrk B, Celik S M. 2014. Pre-harvest spray application of methyl jasmonate plays an important role in fruit ripening,fruit;quality and bioactive compounds of Japanese plums. Scientia Horticulturae, 176:162-169. doi: 10.1016/j.scienta.2014.07.007 URL
[19]	Lelièvre J M, Latchè A, Jones B, Bouzayen M, Pech J C. 1997. Ethylene and fruit ripening. Physiologia Plantarum, 101 (4):727-739. doi: 10.1111/j.1399-3054.1997.tb01057.x URL
[20]	Dong Yin-mao, Yao Xia, Zhang Xiao-hui, Liu Chang, Ren Han-kun. 2014. Research progress on the effect of methyl jasmonate on the metabolism of plant phenolic components. Journal of Chinese Medicinal Materials, 37 (11):2109-2112. (in Chinese)
	李丽, 董银卯, 姚霞, 张小慧, 刘畅, 任晗堃. 2014. 茉莉酸甲酯对植物酚类成分代谢影响研究进展. 中药材, 37 (11):2109-2112.
[21]	Li T, Jiang Z, Zhang L, Tan D M, Wei Y, Yuan H, Wang A D. 2016a. Apple(Malus domestica)MdERF 2 negatively affects ethylene biosynthesis during fruit ripening by suppressing MdACS1 transcription. Plant Journal, 88 (5):735-748. doi: 10.1111/tpj.13289 URL
[22]	Li Tong. 2017. Regulatory mechanism of apple ethylene response factor EFR in ethylene biosythesis during fruit ripening[Ph. D. Dissertation]. Shenyang: Shenyang Agricultural University. (in Chinese)
	李通. 2017. 苹果乙烯应答因子ERF调控果实成熟过程中乙烯合成的机理研究[博士论文]. 沈阳: 沈阳农业大学.
[23]	Li T, Xu Y X, Zhang L, Ji Y L, Tan D M, Yuan H, Wang A D. 2017. The jasmonate-activated transcription factor MdMYC2regulates ETHYLENE RESPONSE FACTOR and ethylene biosynthetic genes to promote ethylene biosynthesis during apple fruit ripening. Plant Cell, 29 (6):1316-1334. doi: 10.1105/tpc.17.00349 URL
[24]	Li Xiang, Sang Qin-qin, Shu Sheng, Sun Jin, Guo Shi-rong. 2016. Effects of epibrassinolide on the activities and gene expression of photosynthetic enzymes in tomato seedlings under low light. Acta Horticulturae Sinica, 43 (10):2012-2020. (in Chinese)
	李翔, 桑勤勤, 束胜, 孙锦, 郭世荣. 2016. 外源油菜素内酯对弱光下番茄幼苗光合碳同化关键酶及其基因的影响. 园艺学报, 43 (10):2012-2020.
[25]	Li X J, Chen X J, Guo X, Yin L L, Ahammed G J, Xu C J, Chen K S, Liu C C, Xia X J, Shi K, Zhou J, Zhou Y H, Yu J Q. 2016b. DWARF overexpression induces alteration in phytohormone homeostasis,development,architecture and carotenoid accumulation in tomato. Plant Biotechnology Journal, 14 (3):1021-1033. doi: 10.1111/pbi.12474 URL
[26]	Li Yuan-yuan. 2017. The effect of 2,4-epibrassionolide treatment on quality maintenance in postharvest strawberry[M. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	李园园. 2017. 2,4-表油菜素内酯处理对草莓果实防腐保鲜效果研究[硕士论文]. 南京: 南京农业大学.
[27]	Liu M, Pirrello J, Chervin C, Roustan J P, Bouzayen M. 2015. Ethylene control of fruit ripening:revisiting the complex network of transcriptional regulation. Plant Physiology, 169:2380-2390.
[28]	Liu W T, Li T, Yuan H, Tan D M, Wang A D. 2018. Enhancement of apple coloration using jasmonate treatment without sacrificing storage potential. Plant Signaling & Behavior, 13 (1):e1422467.
[29]	Liu Xin, Chen Yunzhu, Kim Pyol, Kim Min-Jun, Song Hyondok, Li Yuhua, Wang Yu. 2020. Progress on molecular mechanism and regulation of tomato fruit color formation. Acta Horticulturae Sinica, 47 (9):1689-1704. (in Chinese)
	刘昕, 陈韵竹, Kim Pyol, Kim Min-Jun, Song Hyondok, 李玉花, 王宇. 2020. 番茄果实颜色形成的分子机制及调控研究进展. 园艺学报, 47 (9):1689-1704.
[30]	Lü B, Tian H, Zhang F, Liu J, Lu S, Bai M, Li C, Ding Z. 2018. Brassinosteroids regulate root growth by controlling reactive oxygen species homeostasis and dual effect on ethylene synthesis in Arabidopsis. PLoS Genetics, 14 (1):e1007144. doi: 10.1371/journal.pgen.1007144 URL
[31]	Lü Tian-xiang, Jiang Xiao-jun, Liu Zhi, Liu Wei-ting, Yan Zhong-ye, Huang Jin-feng. 2020. Effect of spraying methyl jasmonate before harvest on the color and internal quality of'Yuehua'apple. Northern Fruits, 21 (2):21-22. (in Chinese)
	吕天星, 姜孝军, 刘志, 刘伟婷, 闫忠业, 黄金凤. 2020. 采前喷施茉莉酸甲酯对'岳华'苹果着色与内在品质的影响. 北方果树,(2):21-22.
[32]	Peng Z, Han C, Yuan L, Zhang K, Huang H, Ren C. 2011. Brassinosteroid enhances jasmonate induced anthocyanin accumulation in Arabidopsis seedlings. Journal of Integrative Plant Biology, 53 (8):632-640. doi: 10.1111/j.1744-7909.2011.01042.x URL
[33]	Qiang Xiao-min, Li Xiu-hong, Li Jin, He Fang-ting, Wang Lin-hai. 2021. China's apple industry in the new situation under the high-quality development of countermeasures. Northwest Horticulture,(2):6-8. (in Chinese)
	强晓敏, 李秀红, 李进, 何芳婷, 王林海. 2021. 新形势下我国苹果产业高质量发展对策. 西北园艺(果树),(2):6-8.
[34]	Sakurai A, Fujioka S. 1993. The current status of physiology and biochemistry of brassinosteroids. Plant Growth Regulation, 13 (2):147-159. doi: 10.1007/BF00024257 URL
[35]	Shan X Y, Zhang Y S, Peng W, Wang Z L, Xie D X. 2009. Molecular mechanism for jasmonate-induction of anthocyanin accumulation in Arabidopsis. Journal of Experimental Botany, 60 (13):3849-3860.
[36]	Sun Xiao-wen. 2016. Effects of jasmonates on coloration and quality of grape berry[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	孙晓文. 2016. 茉莉酸酯类对葡萄果实着色及品质的影响[硕士论文]. 北京: 中国农业科学院.
[37]	Symons G M, Davies C, Shavrukov Y, Dry I B, Reid J B, Thomas M R. 2006. Grapes on steroids. Brassinosteroids are involved in grape berry ripening. Plant Physiol, 140:150-158. pmid: 16361521
[38]	Wang S, Zheng W. 2005. Preharvest application of methyl jasmonate increases fruit quality and antioxidant capacity in raspberries. International Journal of Food Science & Technology, 40 (2):187-195.
[39]	Wu Lixuan, Zhou Ping, Fan Yuxin, Wang Guiqing, Zeng Lihui. 2021. Cloning and functional analysis of NtMYB8 in Narcissus tazetta var. chinensis. Acta Horticulturae Sinica, 48 (10):1895-1906. (in Chinese)
	吴立璇, 周平, 范雨昕, 王桂青, 曾黎辉. 2021. 中国水仙NtMYB8的分离及功能研究. 园艺学报, 48 (10):1895-1906.
[40]	Xu Jian-feng. 2003. Studies on effect of methly jasmonate on colour development of apples[M. D. Dissertation]. Baoding: Hebei Agricultural University. (in Chinese)
	许建锋. 2003. 茉莉酸甲酯对苹果色泽发育效应的研究[硕士论文]. 保定: 河北农业大学.
[41]	Yang Guang-kai, Wu Yuan-li, Gao Yan, Zhang Xiao-jun, Hao Yan-yan. 2021. Effects of exogenous methyl jasmonate on the quality of apple. Journal of Fruit Resources, 2 (5):15-22. (in Chinese)
	杨光凯, 武媛丽, 高燕, 张小军, 郝燕燕. 2021. 外源茉莉酸甲酯对苹果果实品质的影响. 果树资源学报, 2 (5):15-22.
[42]	Yin Bing, He Chun-juan, Liu Feng. 2016. Test of the application effect of 0.01% brassinolide soluble liquid in apple. Shanxi fruits,(4):7-9. (in Chinese)
	尹冰, 贺春娟, 刘凤. 2016. 0.01%芸苔素内酯可溶性液剂在苹果上的应用效果试验. 山西果树,(4):7-9.
[43]	Zaharah S S, Singh Z, Symons G M, Reid J B. 2011. Role of brassinosteroids,ethylene,abscisic acid,and indole-3-acetic acid in mango fruit ripening. J Plant Growth Regul, 31:363-372. doi: 10.1007/s00344-011-9245-5 URL
[44]	Zhang Meng-yuan. 2019. Effects of MeJA on quality and expression of key genes involved in ethylene biosynthesis and signal transduction pathway during postharvest ripening of apple fruit[M. D. Dissertation]. Jinzhou: Bohai University. (in Chinese)
	张梦媛. 2019. MeJA对采后苹果果实品质和乙烯生物合成及其信号转导途径关键基因表达的影响[硕士论文]. 锦州: 渤海大学.
[45]	Zhang Wei-Long, Liang Fa-hui, Yang Jing-hui, Li Bing, Zhang Chao, Zhang Qi. 2021. Effects of nutrients and plant growth regulators on grape fruit splitting and fruit quality. Northern Horticulture,(5):34-41. (in Chinese)
	张伟龙, 梁发辉, 杨静慧, 李冰, 张超, 张琦. 2021. 营养元素与植物生长调节剂对葡萄裂果及果实品质的影响. 北方园艺,(5):34-41.
[46]	Zhang Xin-hao, Wang Yao, Wang Hui-dong, Sang Ya-li, Liu Yan-hua, Li Xiao-chun. 2018. Determination of Vc content in vegetables. Journal of Chifeng University(Natural Science Edition), 34 (3):29-31. (in Chinese)
	张歆皓, 王瑶, 王会东, 桑雅丽, 刘艳华, 李晓春. 2018. 蔬菜中Vc含量的测定. 赤峰学院学报(自然科学版), 34 (3):29-31.
[47]	Zheng J, An Y, Wang L. 2018. 24-Epibrassinolide enhances 5-ALA-induced anthocyanin and flavonol accumulation in calli of'Fuji'apple flesh. The Plant Cell Tissue and Organ Culture, 134 (2):319-330. doi: 10.1007/s11240-018-1418-5 URL
[48]	Zhong Chunmei, Wang Xiaojing. 2021. Advance in research on regulation of flower pigmentation in Gerbera hybrida. Acta Horticulturae Sinica, 48 (10):2031-2043. (in Chinese)
	钟春梅, 王小菁. 2021. 非洲菊花色形成调控研究进展. 园艺学报, 48 (10):2031-2043.
[49]	Zhu T, Tan W R, Deng X G, Zheng T, Zhang D W, Lin H H. 2015. Effects of brassinosteroids on quality attributes and ethylene synthesis in postharvest tomato fruit. Postharvest Biology and Technology, 100:196-204. doi: 10.1016/j.postharvbio.2014.09.016 URL
[50]	Zhu Z, Zhang Z, Qin G, Tian S. 2010. Effects of brassinosteroids on postharvest disease and senescence of jujube fruit in storage. Postharvest Biol Technol, 56:50-55. doi: 10.1016/j.postharvbio.2009.11.014 URL

Effects of Brassinolides and Methyl Jasmonate Spraying on the Postharvest Quality of Apple Fruit

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