PpyERF060-PpyABF3-PpyMADS71 Regulates Ethylene Signaling Pathway- Mediated Pear Bud Dormancy Process

doi:10.16420/j.issn.0513-353x.2022-0585

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (10): 2249-2262.doi: 10.16420/j.issn.0513-353x.2022-0585

• Research Papers • Previous Articles Next Articles

PpyERF060-PpyABF3-PpyMADS71 Regulates Ethylene Signaling Pathway- Mediated Pear Bud Dormancy Process

YANG Bo¹, WEI Jia¹, LI Kunfeng², WANG Chengliang³, NI Junbei¹, TENG Yuanwen¹^,⁴, and BAI Songling¹^,^*()

¹Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China
²Agricultural Experiment Station,Zhejiang University,Hangzhou 310058,China
³Wuxi Agricultural Technology Extension Center,Wuxi,Jiangsu 214000,China
⁴Hainan Institute of Zhejiang University,Sanya,Hainan 572000,China

Received:2022-06-08 Revised:2022-08-22 Online:2022-10-25 Published:2022-10-31
Contact: and BAI Songling E-mail:songlingbai@zju.edu.cn

Abstract

Abstract:

In order to clarify the specific regulation mode of DAM gene in dormancy,'Dangshan Suli'pear floral buds were used as the material to explore the upstream transcriptional factors of a DAM gene PpyMADS71 identified in previous studies. The results are as follows：(1) PpyERF060（Ethylene responsive factor 060）and PpyMADS71 were co-expressed during bud dormancy cycle. The yeast binding site mutation assays and the dual-luciferase assays were used to confirm that PpyERF060 could bind to the DRE1 element of the PpyMADS71 promoter and activates PpyMADS71's expression,which was further proved by the upregulation of PpyMADS71 expression in PpyERF060 transgenic pear calli. (2) To explore the upstream genes of PpyERF060,we found that the transcription factor PpyABF3（ABRE BINDING FACTOR 3）could activate the expression of PpyERF060 and the binding site of PpyABF3 on the promoter of PpyERF060 was primarily determined. (3) Furthermore,ethylene treatment on'Dangshan Suli'buds caused up-regulation of PpyERF060 and down-regulation of PpyABF3. In addition,overexpression of PpyERF060 in pear calli caused inhibition of the transcription of PpyABF3. Based on the above results,we found that there is a regulatory network among PpyERF060,PpyABF3,and PpyMADS71,which could combine ethylene and ABA signaling pathway,and ultimately regulates the dormancy process.

Key words: pear, bud, dormancy, ethylene, transcriptional regulation, DAM

CLC Number:

S661.1

YANG Bo, WEI Jia, LI Kunfeng, WANG Chengliang, NI Junbei, TENG Yuanwen, and BAI Songling. PpyERF060-PpyABF3-PpyMADS71 Regulates Ethylene Signaling Pathway- Mediated Pear Bud Dormancy Process[J]. Acta Horticulturae Sinica, 2022, 49(10): 2249-2262.

Figures/Tables 11

References 45

[1]	Arc E, Sechet J, Corbineau F, Rajjou L, Marion-Poll A. 2013. ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Frontiers in Plant Science, 3 (4):63.
[2]	Arora R, Rowland L J, Tanino K. 2003. Induction and release of bud dormancy in woody perennials:a science comes of age. HortScience, 38 (5):911-921. doi: 10.21273/HORTSCI.38.5.911 URL
[3]	Baldocchi D, Wong S. 2008. Accumulated winter chill is decreasing in the fruit growing regions of California. Climatic Change, 87 (1S):153-166. doi: 10.1007/s10584-007-9367-8 URL
[4]	Bielenberg D G, Wang Y E, Li Z, Zhebentyayeva T, Fan S, Reighard G L, Scorza R, Abbott A G. 2008. Sequencing and annotation of the evergrowing locus in peach [Prunus persica(L.)Batsch] reveals a cluster of six MADS-box transcription factors as candidate genes for regulation of terminal bud formation. Tree Genetics & Genomes, 4 (3):495-507.
[5]	Campoy J A, Ruiz D, Egea J. 2011. Dormancy in temperate fruit trees in a global warming context:a review. Scientia Horticulturae(Amsterdam), 130 (2):357-372.
[6]	Cooke J E, Eriksson M E, Junttila O. 2012. The dynamic nature of bud dormancy in trees:environmental control and molecular mechanisms. Plant,Cell and Environment, 35 (10):1707-1728. doi: 10.1111/j.1365-3040.2012.02552.x URL
[7]	Corbineau F, Xia Q, Bailly C, El-Maarouf-Bouteau H. 2014. Ethylene,a key factor in the regulation of seed dormancy. Frontiers in Plant Science, 5:539.
[8]	El-Maarouf-Bouteau H, Sajjad Y, Bazin J, Langlade N, Cristescu S M, Balzergue S, Baudouin E, Bailly C. 2015. Reactive oxygen species,abscisic acid and ethylene interact to regulate sunflower seed germination. Plant,Cell and Environment, 38:364-374. doi: 10.1111/pce.12371 URL
[9]	Feehan J, Harley M, Minnen J V. 2009. Climate change in Europe. 1. Impact on terrestrial ecosystems and biodiversity. A review. Agronomy for Sustainable Development, 29 (3):409-421. doi: 10.1051/agro:2008066 URL
[10]	Fu J R, Yang S F. 1983. Release of heat pretreatment-induced dormancy in lettuce seeds by ethylene or cytokinin in relation to the production of ethylene and the synthesis of 1-aminocyclopropane-1-carboxylic acid during germination. Journal of Plant Growth Regulation, 2 (1-4):185. doi: 10.1007/BF02042229 URL
[11]	Horvath D P, Anderson J V, Chao W S, Foley M E. 2003. Knowing when to grow:signals regulating bud dormancy. Trends in Plant Science, 8 (11):534-540. pmid: 14607098
[12]	Horvath D P, Sung S, Kim D, Chao W, Anderson J. 2010. Characterization,expression and function of DORMANCY ASSOCIATED MADS-BOX genes from leafy spurge. Plant Molecular Biology, 73 (1-2):169-179. doi: 10.1007/s11103-009-9596-5 pmid: 20066557
[13]	Howe G T, Horvath D P, Dharmawardhana P, Priest H D, Mockler T C, Strauss S H. 2015. Extensive Transcriptome changes during natural onset and release of vegetative bud dormancy in Populus. Frontiers in Plant Science, 6:989.
[14]	Irena Sińska. 1989. Interaction of ethephon with cytokinin and gibberellin during the removal of apple seed dormancy and germination of embryos. Plant Science, 64 (1):39-44. doi: 10.1016/0168-9452(89)90149-0 URL
[15]	Kilany A E, Hegazi E S, Kilany O A. 1990. Ethylene evolution,CO₂ product-ion and some endogenous compounds in relation to bud dormancy of pear trees. Bulletin of Faculty of Agriculture, 38 (2):357-367.
[16]	Kucera B, Cohn M A, Leubner-Metzger G. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15 (4):281-307. doi: 10.1079/SSR2005218 URL
[17]	Lang G A, Early J D, Martin G C, Daenell R L. 1987. Endo-,para-,and ecodormancy:physiological terminology and classification for dormancy research. HortScience, 22 (3):371-377. doi: 10.21273/HORTSCI.22.3.371 URL
[18]	Li J, Xu Y, Niu Q, He L, Teng Y, Bai S. 2018. Abscisic acid(ABA)promotes the induction and maintenance of pear(Pyrus pyrifolia white pear group)flower bud endodormancy. International Journal of Molecular Sciences, 19 (1):310. doi: 10.3390/ijms19010310 URL
[19]	Li Jian-zhao. 2019. Studies on the molecular mechanism of ABA and related phytohormones-regulated pear bud dormancy[Ph. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	李建召. 2019. 脱落酸及相关激素调控梨芽休眠的分子机制研究[博士论文]. 杭州: 浙江大学.
[20]	Li P, Zheng T, Zhuo X, Zhang M, Yong X, Li L, Wang J, Cheng T, Zhang Q. 2021. Photoperiod- and temperature-mediated control of the ethylene response and winter dormancy induction in Prunus mume. Horticultural Plant Journal, 7 (3):232-242. doi: 10.1016/j.hpj.2021.03.005 URL
[21]	Linkies A, Muller K, Morris K, Turečková V, Wenk M, Cadman C S C, Corbineau F, Strnad M, Lynn J R, Finch-Savage W E, Leubner-Metzger G. 2009. Ethylene interacts with abscisic acid to regulate endosperm rupture during germination:a comparative approach using Lepidium sativum and Arabidopsis thaliana. The Plant Cell, 21 (12):3803-3822. doi: 10.1105/tpc.109.070201 pmid: 20023197
[22]	Niu Qing-feng. 2016. The molecular mechanism and molecular network of pear flower bud dormancy transition[Ph. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	牛庆丰. 2016. 梨花芽休眠转换的分子网络及调控机制[博士论文]. 杭州: 浙江大学.
[23]	Ophir R, Pang X, Halaly T, Venkateswari J, Lavee S, Galbraith D, Or E. 2009. Gene-expression profiling of grape bud response to two alternative dormancy-release stimuli expose possible links between impaired mitochondrial activity,hypoxia,ethylene-ABA interplay and cell enlargement. Plant Molecular Biology, 71 (4-5):403-423. doi: 10.1007/s11103-009-9531-9 pmid: 19653104
[24]	Porto D D, Falavigna V d S, Arenhart R A, Perini P, Buffon V, Anzanello R, dos Santos H P, Fialho F B, Dias de Oliveira P R, Revers L F. 2016. Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple. Tree Genetics & Genomes, 12 (3). Doi: 10.1007/s11295-016-1001-3. doi: 10.1007/s11295-016-1001-3 URL
[25]	Qin Li-jin, Xu Zhen-jun, Yuan Shu-xiang. 2007. Effects of seed soaking with ethephon on seed germination and seedling growth of cucumber in greenhouse in winter. Journal of Anhui Agricultural Sciences,(33):10601-10602. (in Chinese)
	秦立金, 徐振军, 袁树祥. 2007. 乙烯利浸种对冬季日光温室黄瓜种子萌发与幼苗生长的影响. 安徽农业科学,(33):10601-10602.
[26]	Rohde A, Ruttink T, Hostyn V, Sterck L, Van Driessche K, Boerjan W. 2007. Gene expression during the induction,maintenance,and release of dormancy in apical buds of poplar. Journal of Experimental Botany, 58 (15-16):4047-4060. doi: 10.1093/jxb/erm261 URL
[27]	Ruonala R, Rinne P L H, Baghour M, Moritz T, Tuominen H, Kangasjarvi J. 2006. Transitions in the functioning of the shoot apical meristem in birch(Betula pendula)involve ethylene. Plant Journal, 46 (4):628-640. pmid: 16640599
[28]	Ruttink T, Arend M, Morreel K, Storme V, Rombauts S, Fromm J, Bhalerao R P, Boerjan W, Rohde A. 2007. A molecular timetable for apical bud formation and dormancy induction in poplar. The Plant Cell Online, 19 (8):2370-2390. doi: 10.1105/tpc.107.052811 URL
[29]	Samish M R. 1954. Dormancy in woody plants. Annual Review of Plant Physiology, 5 (1):183-204. doi: 10.1146/annurev.pp.05.060154.001151 URL
[30]	Sasaki R, Yamane H, Ooka T, Jotatsu H, Kitamura Y, Akagi T, Tao R. 2011. Functional and expressional analyses of PmDAM genes associated with endodormancy in Japanese apricot. Plant Physiology, 157 (1):485-497. doi: 10.1104/pp.111.181982 pmid: 21795580
[31]	Shao Xu. 2016. Cloning and functional analysis of dormancy-associated transcription factor(MADS-box)from Chinese cherry flower buds[M. D. Dissertation]. Hangzhou: Zhejiang Normal University. (in Chinese)
	邵姁. 2016. 中国樱桃花芽休眠相关MADS-box转录因子克隆与功能分析[硕士论文]. 杭州: 浙江师范大学.
[32]	Shi Z, Halaly-Basha T, Zheng C, Weissberg M, Ophir R, Galbraith D W, Pang X, Or E. 2018. Transient induction of a subset of ethylene biosynthesis genes is potentially involved in regulation of grapevine bud dormancy release. Plant Molecular Biology, 98 (6):507-523. doi: 10.1007/s11103-018-0793-y pmid: 30392158
[33]	Singh R K, Maurya J P, Azeez A, Miskolczi P, Tylewicz S, Stojkovič K, Delhomme N, Busov V, Bhalerao R P. 2018. A genetic network mediating the control of bud break in hybrid aspen. Nature Communications, 9:4173. doi: 10.1038/s41467-018-06696-y pmid: 30301891
[34]	Subbiah V, Reddy K J. 2010. Interactions between ethylene,abscisic acid and cytokinin during germination and seedling establishment in Arabidopsis. Journal of Bioscience and Bioengineering, 35 (3):451-458.
[35]	Song Song-quan, Liu Jun, Xu Heng-heng, Zhang Qi, Huang Hui, Wu Xian-jin. 2019. Biosynthesis and signaling of ethylene and their regulation on seed germination and dormancy. Acta Agronomica Sinica, 45 (7):969-981. (in Chinese) doi: 10.3724/SP.J.1006.2019.84175
	宋松泉, 刘军, 徐恒恒, 张琪, 黄荟, 伍贤进. 2019. 乙烯的生物合成与信号及其对种子萌发和休眠的调控. 作物学报, 45 (7):969-981. doi: 10.3724/SP.J.1006.2019.84175
[36]	Tian Wen-jie. 2018. Effect of ethephon soaking on seed germination of maize. Modern Agricultural Science and Technology,(15):1-2. (in Chinese)
	田文杰. 2018. 乙烯利浸种对玉米种子萌发的影响. 现代农业科技,(15):1-2.
[37]	Ubi B E, Sakamoto D, Ban Y, Shimada T, Ito A, Nakajima I, Takemura Y, Tamura F, Saito T, Moriguchi T. 2010. Molecular cloning of dormancy-associated MADS-box gene homologs and their characterization during seasonal endodormancy transitional phases of Japanese pear. Journal of the American Society for Horticultural Science, 135 (2):174-182. doi: 10.21273/JASHS.135.2.174 URL
[38]	Wu R M, Walton E F, Richardson A C, Wood M, Hellens R P, Varkonyi-Gasic E. 2012. Conservation and divergence of four kiwifruit SVP-like MADS-box genes suggest distinct roles in kiwifruit bud dormancy and flowering. Journal of Experimental Botany, 63 (2):797-807. doi: 10.1093/jxb/err304 URL
[39]	Wu R, Wang T, McGie T, Voogd C, Allan A C, Hellens R P, Varkonyi-Gasic E. 2014. Overexpression of the kiwifruit SVP3 gene affects reproductive development and suppresses anthocyanin biosynthesis in petals,but has no effect on vegetative growth,dormancy,or flowering time. Journal of Experimental Botany, 65 (17):4985-4995. doi: 10.1093/jxb/eru264 URL
[40]	Wu R, Wang T, Warren B A W, Allan A C, Macknight R C, Varkonyi-Gasic E. 2017. Kiwifruit SVP2 gene prevents premature budbreak during dormancy. Journal of Experimental Botany, 68 (5):1071-1082. doi: 10.1093/jxb/erx014 URL
[41]	Yamane H, Kashiwa Y, Ooka T, Tao R, Yonemori K. 2008. Suppression subtractive hybridization and differential screening reveals endodormancy-associated expression of an SVP/AGL24-type MADS-box gene in lateral vegetative buds of Japanese apricot. Journal of the American Society for Horticultural Science, 133 (5):708-716. doi: 10.21273/JASHS.133.5.708 URL
[42]	Yan Xin-hui. Identification and functional analysis of pear dormancy associated DAM genes[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	鄢馨卉. 2019. 梨休眠相关DAM基因的鉴定及功能分析[硕士论文]. 杭州: 浙江大学.
[43]	Yang Q, Niu Q, Li J, Zheng X, Ma Y, Bai S, Teng Y. 2018. PpHB22,a member of HD-Zip proteins,activates,PpDAM1,to regulate bud dormancy transition in'Suli'pear(Pyrus pyrifolia white pear group). Plant Physiology and Biochemistry, 127:355-365. doi: 10.1016/j.plaphy.2018.04.002 URL
[44]	Yang Q, Yang B, Li J, Wang Y, Tao R, Yang F, Wu X, Yan X, Ahmad M, Shen J, Bai S, Teng Y. 2020. ABA-responsive ABRE-BINDING FACTOR3 activates DAM3 expression to promote bud dormancy in Asian pear. Plant,Cell and Environment, 43 (6):1360-1375. doi: 10.1111/pce.13744 URL
[45]	Zheng C, Acheampong A K, Shi Z, Mugzech A, Halaly-Basha T, Shaya F, Sun Y, Colova V, Mosquna A, Ophir R, Galbraith D W, Or E. 2018. Abscisic acid catabolism enhances dormancy release of grapevine buds. Plant,Cell and Environment, 41 (10):2490-2503. doi: 10.1111/pce.13371 URL

用途 Use	引物 Primer	序列 Sequence
定量表达	Q-PpyERF060-F	TCGGATTCTCAGTGGGACGA
qRT-PCR	Q-PpyERF060-R	TTACAGCATCACCACGCCTT
	Q-PpyMADS71-F	AACAACCAGCTAAGCCAGAAG
	Q-PpyMADS71-R	TCAAGGGTTGGAGAAGGTGG
	Q-PpyABF3-F	CCGGTTCGAATTTGCCACAG
	Q-PpyABF3-R	CGGTTGTTGAAAGCCGGAAG
亚细胞定位	PpyERF060-GFP-F	AGCTCGGTACCCGGGATGGCAACATCCATAGATCTTTAC
Subcellular localization	PpyERF060-GFP-R	CATGTCGACTCTAGAGATAGCAGACCAATCAATCTCCA
酵母单杂	pMADS71-pAbAi-F	GAAAAGCTTGAATTCGAGCTCGGGTGACTGGTTGCTGGTTGTT
Y1H assay	pMADS71-mutDRE1-pAbAi-R	ACGTGGAGTTTTTCACACCT
	pMADS71-mutDRE1-pAbAi-F	AGGTGTGAAAAACTCCACGT
	pMADS71-mutDRE2-pAbAi-R	GTTTATTGTTTTTTTAGCCT
	pMADS71-mutDRE2-pAbAi-F	AGGCTAAAAAAACAATAAAC
	pMADS71-pAbAi-R	AGCACATGCCTCGAGGTCGACCAACGGTGATCGATGCGGTTGG
双荧光素酶	pMADS71-all-LUC-F	TCGAGGTCGACGGTATCGATACCTAAAGCGCAACCGTAACTG
Dual-Luciferase assay	pMADS71-all-LUC-R	CCGCTCTAGAACTAGTGGATCCTTCTCTTTCTTCCTACCCCCT
	PpyERF060-SK-F	GCGGCCGCTCTAGAACTAGTGATGGCAACATCCATAGATCTTTAC
	PpyERF060-SK-R	GGTCGACGGTATCGATAAGCTTTAGATAGCAGACCAATCAATCTC
	pMADS71-delDRE1-LUC-F	TCGAGGTCGACGGTATCGATATCCACGTGAACTCGGATAAACACC
	pMADS71-mutDRE1-LUC-R	ACGTGGAGTTTTTCACACCT
	pMADS71-mutDRE1-LUC-F	AGGTGTGAAAAACTCCACGT
	pERF060-LUC-F	TCGAGGTCGACGGTATCGATATAAAAAAGTTAGGGCTAAAATTGT
	pERF060-LUC-R	CCGCTCTAGAACTAGTGGATCTTAGATTTAGTCACAAAAGATAAA
	PpyABF3-SK-F	GCGGCCGCTCTAGAACTAGTGATGGGGTCTAATTTCAACTTCAA
	PpyABF3-SK-R	GGTCGACGGTATCGATAAGCTTTACCAAGGGCCTGTCAATGT
PpyERF060过表达	PpyERF060-1301-F	AGAACACGGGGGACTCTTGACATGGCAACATCCATAGATCTTTAC
PpyERF060 overexpression	PpyERF060-1301-R	GGGGAAATTCGAGCTGGTCACTTAGATAGCAGACCAATCAATCTC

用途 Use	引物 Primer	序列 Sequence
定量表达	Q-PpyERF060-F	TCGGATTCTCAGTGGGACGA
qRT-PCR	Q-PpyERF060-R	TTACAGCATCACCACGCCTT
	Q-PpyMADS71-F	AACAACCAGCTAAGCCAGAAG
	Q-PpyMADS71-R	TCAAGGGTTGGAGAAGGTGG
	Q-PpyABF3-F	CCGGTTCGAATTTGCCACAG
	Q-PpyABF3-R	CGGTTGTTGAAAGCCGGAAG
亚细胞定位	PpyERF060-GFP-F	AGCTCGGTACCCGGGATGGCAACATCCATAGATCTTTAC
Subcellular localization	PpyERF060-GFP-R	CATGTCGACTCTAGAGATAGCAGACCAATCAATCTCCA
酵母单杂	pMADS71-pAbAi-F	GAAAAGCTTGAATTCGAGCTCGGGTGACTGGTTGCTGGTTGTT
Y1H assay	pMADS71-mutDRE1-pAbAi-R	ACGTGGAGTTTTTCACACCT
	pMADS71-mutDRE1-pAbAi-F	AGGTGTGAAAAACTCCACGT
	pMADS71-mutDRE2-pAbAi-R	GTTTATTGTTTTTTTAGCCT
	pMADS71-mutDRE2-pAbAi-F	AGGCTAAAAAAACAATAAAC
	pMADS71-pAbAi-R	AGCACATGCCTCGAGGTCGACCAACGGTGATCGATGCGGTTGG
双荧光素酶	pMADS71-all-LUC-F	TCGAGGTCGACGGTATCGATACCTAAAGCGCAACCGTAACTG
Dual-Luciferase assay	pMADS71-all-LUC-R	CCGCTCTAGAACTAGTGGATCCTTCTCTTTCTTCCTACCCCCT
	PpyERF060-SK-F	GCGGCCGCTCTAGAACTAGTGATGGCAACATCCATAGATCTTTAC
	PpyERF060-SK-R	GGTCGACGGTATCGATAAGCTTTAGATAGCAGACCAATCAATCTC
	pMADS71-delDRE1-LUC-F	TCGAGGTCGACGGTATCGATATCCACGTGAACTCGGATAAACACC
	pMADS71-mutDRE1-LUC-R	ACGTGGAGTTTTTCACACCT
	pMADS71-mutDRE1-LUC-F	AGGTGTGAAAAACTCCACGT
	pERF060-LUC-F	TCGAGGTCGACGGTATCGATATAAAAAAGTTAGGGCTAAAATTGT
	pERF060-LUC-R	CCGCTCTAGAACTAGTGGATCTTAGATTTAGTCACAAAAGATAAA
	PpyABF3-SK-F	GCGGCCGCTCTAGAACTAGTGATGGGGTCTAATTTCAACTTCAA
	PpyABF3-SK-R	GGTCGACGGTATCGATAAGCTTTACCAAGGGCCTGTCAATGT
PpyERF060过表达	PpyERF060-1301-F	AGAACACGGGGGACTCTTGACATGGCAACATCCATAGATCTTTAC
PpyERF060 overexpression	PpyERF060-1301-R	GGGGAAATTCGAGCTGGTCACTTAGATAGCAGACCAATCAATCTC

PpyERF060-PpyABF3-PpyMADS71 Regulates Ethylene Signaling Pathway- Mediated Pear Bud Dormancy Process

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[15]	LIU Jinming, GUO Caihua, YUAN Xing, KANG Chao, QUAN Shaowen, NIU Jianxin. Genome-wide Identification of Dof Family Genes and Expression Analysis Sepal Persistent and Abscission in Pear [J]. Acta Horticulturae Sinica, 2022, 49(8): 1637-1649.