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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1895-1906.doi: 10.16420/j.issn.0513-353x.2021-0700

• Research Papers • Previous Articles     Next Articles

Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena

HU Ruolin1, WANG Jiali1, YANG Huiqin1, YUAN Chao1, NIU Yi1,*(), TANG Qinglin1, WEI Dayong1, TIAN Shibing2, YANG Yang2, WANG Zhimin1,*()   

  1. 1Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
    2The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China
  • Received:2021-10-16 Revised:2022-05-17 Online:2022-09-25 Published:2022-10-08
  • Contact: NIU Yi,WANG Zhimin E-mail:niuy2001134@163.com;minzniwang_555@163.com

Abstract:

SmARF5 gene was cloned from the eggplant cultivar‘S12’. The open reading frame is 2 793 bp,and it encodes a 930 aa protein that located in the nucleus. The pGBKT7-SmARF5-Full and pGBKT7-SmARF5-674aa vectors were constructed. The transcriptional activation activity of SmARF5 protein was analyzed and showed by yeast transformation. The full length of SmARF5 could interact with SmIAA16 and SmIAA26 proteins. The truncated SmARF5-674aa did not interact with SmIAA16 or SmIAA26. The pCAMBIA-2301G-SmARF5 overexpression vector was constructed and genetically transformed into tobacco plants. Compared with the wild type,obvious branches and the stem thickness were increased in the transgenic plants. The above results indicated that the SmARF5 transcription factor might play an important role in the formation of plant branches through the auxin signal transduction pathway.

Key words: Solanum melongena, SmARF5, auxin, branching

CLC Number: