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Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 170-176.doi: 10.16420/j.issn.0513-353x.2021-0806

• Research Notes • Previous Articles     Next Articles

Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum

HU Jingyu1, QUE Kaijuan1, MIAO Tianli1, WU Shaozheng2, WANG Tiantian2, ZHANG Lei1, DONG Xian1, JI Pengzhang1, DONG Jiahong1,*()   

  1. 1School of Chinese Materia Medica and Yunnan,Key Laboratory of Southern Medicinal Resource,Yunnan University of Chinese Medicine,Kunming 650500,China
    2Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China
  • Received:2022-03-25 Revised:2022-07-11 Online:2023-01-25 Published:2023-01-18
  • Contact: *(E-mail:dongjhn@126.com

Abstract:

In 2020,Iris tectorum Maxim plants exhibiting chlorotic spot and necrotic ringspot symptoms were observed on campus in Yunnan University of Chinese Medicine in Kunming City,Yunnan Province. The typical virions of orthotospoviruses were observed by electron microscope. The systemic necrotic spots symptoms were found on the plants of Nicotiana tabacum and N. benthamiana inoculated mechanically with the sap of the symptomatic I.tectorum Maxim leaves. The inoculated leaves of Capsicum chinense Jacquin PI152225 showed the local lethal spots,whilst no systemic infection were detected. To further confirm the pathogen,RT-PCR were performed to amplify the NSs and N genes of tomato spotted wilt orthotospovirus(TSWV),respectively. Sequence analysis showed the obtained sequences had the highest identities(99.79% and 100%)with NSs and N genes of TSWV isolates infecting tomato and Tropaeolum majus in Yunnan Province. The results indicated TSWV infection causes the chlorotic spots and necrotic ringspots sympt,oms in I. tectorum.

Key words: Iris tectorum, tomato spotted wilt orthotospovirus, N gene, NSs gene

CLC Number: