Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

doi:10.16420/j.issn.0513-353x.2021-0764

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (11): 2293-2303.doi: 10.16420/j.issn.0513-353x.2021-0764

• Research Papars • Next Articles

Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

WANG Zhiyu¹^,², CHANG Beibei¹^,², LIU Qi¹^,², CHENG Xiaofan¹^,², DU Xiaoyun¹, YU Xiaoli¹, SONG Laiqing¹^,^*(), ZHAO Lingling¹^,^*()

¹Key Laboratory of Apple Germplasm Innovation and Quality Control of Yantai，Yantai Academy of Agricultural Sciences，Yantai，Shandong 265500，China
²College of Life Sciences，Yantai University，Yantai，Shandong 264005，China

Received:2022-06-06 Revised:2022-07-15 Online:2022-11-25 Published:2022-11-25
Contact: SONG Laiqing,ZHAO Lingling E-mail:sdslq@126.com;zhaolingling122@163.com

Abstract

Abstract:

Anthocyanin is the main pigment affecting the skin color of apple fruit. In our previous study，MdSLC35F2-like was expressed at higher levels in the‘Nagafu 2’apple red bud mutant than in the wild-type. The open reading frame（ORF）of MdSLC35F2-like was cloned，it is 1 041bp，encoding 346 amino acids，which was predicted to be a hydrophobic α helix transmembrane protein with the highest homology to the PbSLC35F1-like of Pyrus × bretschneideri. Phylogenetic analysis showed that it had the closest evolutionary relationship with P. × bretschneideri，and the furthest evolutionary relationship with Theobroma cacao and Eucalyptus grandis. MdSLC35F2-like sequence and its expression rules before and after the fruit bagging were analyzed. After unbagging，the expression level of MdSLC35F2-like increased firstly，and then decreased with a peak on the third day. The expression level of MdSLC35F2-like in the two kinds of buds was significantly higher than that of‘Nagafu 2’. The above results indicated that the expression level of MdSLC35F2-like gene is positively correlated with anthocyanine content. Meanwhile，its expression levels in different color apple cultivars were compared. The expression of MdSLC35F2-like in red peel cultivars was significantly higher than that in green peel cultivars，and the expression level in red pulp cultivars was higher than that in non-red pulp cultivars. It suggests that MdSLC35F2-like may participate in the anthocyanine accumulation process.

Key words: apple, anthocyanin, MdSLC35F2-like expression analysis

CLC Number:

S661.1

WANG Zhiyu, CHANG Beibei, LIU Qi, CHENG Xiaofan, DU Xiaoyun, YU Xiaoli, SONG Laiqing, ZHAO Lingling. Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple[J]. Acta Horticulturae Sinica, 2022, 49(11): 2293-2303.

Figures/Tables 9

Fig. 1 Gene cloning of MdSLC35F2-like

Fig. 2 MdSLC35F2-like is compared by protein homology sequence XP_0072029：Prunus persica;XP_0342249：Prunus dulcis;XP_0082412：Prunus mume;XP_0218101：Prunus avium;CAB4287212：Prunus armeniaca;XP_0093666：Pyrus × bretschneideri;KAB2607766：Pyrus ussuriensis × Pyrus communis;XP_0289478：Malus × domestica.

Fig. 3 Cluster analysis of MdSLC35F2-like amino acid and other plant amino acid sequences

Fig. 4 Pigmentation changes of pericarp after unbagging in‘Nagafu 2’wild type and its slices of red buds

Fig. 5 Content of anthocyanin in‘Nagafu 2’wild type and its slices of red buds * P < 0.05，** P < 0.01.

Fig. 6 Expression of MdSLC35F2-like in‘Nagafu 2’wild type and its slices of red buds * P < 0.05，** P < 0.01.

Fig. 7 Correlation analysis between anthocyanin in content and MdSLC35F2-like expression level

Fig. 8 Content of anthocyanin and expression of MdSLC35F2-like in‘Jinling’（green peel）and‘Yanfu 3’（red peel） ** P < 0.01.

Fig. 9 Content of anthocyanin and expression of MdSLC35F2-like in‘Yannong Zaofu’（white pulp）and‘Red Love’（red pulp） * P < 0.05;** P < 0.01.

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Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

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