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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1785-1794.doi: 10.16420/j.issn.0513-353x.2021-0418

• Research Notes • Previous Articles     Next Articles

Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan

CHEN Lilang1, YANG Tianzhang1, CAI Ruping1, LIN Xiaoman1, DENG Nankang1, CHE Haiyan2, LIN Yating2, KONG Xiangyi1,*()   

  1. 1. Sanya Academy of Tropical Agricultural Sciences,Sanya,Hainan 572000,China
    2. Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
  • Received:2022-04-14 Revised:2022-08-16 Online:2022-08-25 Published:2022-09-05
  • Contact: KONG Xiangyi E-mail:kongxiangyi20@163.com

Abstract:

A total of 176 Passiflora edulis leaf samples showing mosaic,crinkle and deformation were collected from Sanya and Wuzhishan of Hainan Province from May to July 2020. Seven viruses were detected by PCR and RT-PCR including cucumber mosaic virus ( CMV ),cucurbit aphid-borne yellows virus ( CABYV ),East Asian Passiflora virus ( EAPV ),Telosma mosaic virus ( TeMV ),passion fruit woodiness virus ( PWV ),Passiflora mottle virus ( PaMV ) and tomato yellow leaf curl virus ( TYLCV ). The result showed that TeMV was the most prevalent virus with infection rate of 52.27%,followed by PaMV(43.75%),CMV and EAPV ( 28.98% ),PWV ( 22.73% ),TYLCV ( 9.09% ) and CABYV ( 4.55% ). TeMV,PaMV,CMV,EAPV and PWV may be the main viruses infecting P. edulis in Sanya and Wuzhishan. 78.91% of samples were detected co-infection with a total of 32 kinds of co-infection types,in which co-infection of two to four viruses commonly occurs. Three complete genome sequences of TYLCV from P. edulis were determined to be 2 781 bp and encoded six open reading frames ( ORFs ). Both the identity and phylogenetic analysis based on the complete genome showed that all the three TYLCV isolates belonged to TYLCV Israel strain with nt identity ranging from 98.85% to 99.14%,and clustered in the same branch with the four TYLCV isolates from other crops in Hainan. To our knowledge,this is the first report of TYLCV in P. edulis.

Key words: Passiflora edulis, virus disease, pathogen detection, tomato yellow leaf curl virus

CLC Number: