Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 465-476.doi: 10.16420/j.issn.0513-353x.2020-0468

• Research Papers • Previous Articles     Next Articles

VvMYB6,an R2R3-MYB Transcription Factor,is Involved in Anthocyanin Biosynthesis of Grapevine

ZHU Ziguo1, ZHANG Qingtian1, HAN Zhen1, LI Bo1, LI Guodong2, LI Xiujie1,*()   

  1. 1Shandong Institute of Pomology,Tai'an,Shandong 271000,China
    2Shandong Fruit & Tea Technology Services,Ji’nan 250013,China
  • Received:2020-11-24 Online:2021-03-25 Published:2021-04-02
  • Contact: LI Xiujie


Here,we demonstrated that VvMYB6,an R2R3-MYB transcription factor,may function as an activator of anthocyanin biosynthesis in grapevine(Vitis vinifera L.). The VvMYB6 protein contains a conserved R2R3 domain at its N-terminal and localized to the nucleus. Expression studies in grapevine showed that VvMYB6 were strongly expressed in roots,flowers,and berry development of early stages. Ectopic expression of VvMYB6 in tobacco caused the accumulation of anthocyanins in petals and stamens. Metabolomics analysis showed that VvMYB6 expression also caused a marked accumulation of several phenolic compounds,including delphinidin and cyanidin. Expression analysis revealed that several anthocyanin biosynthesis structural genes,including chalcone synthease(CHS),chalcone isomerase(CHI),dihydroflavonol 4-reductase(DFR),anthocyanidin synthase(ANS)and UDP glucose flavonoid 3-O-glucosyl transferase(UFGT),were strongly up-regulated in transgenic plants. These findings suggest that VvMYB6 plays a key role in anthocyanin biosynthesis.

Key words: grapevine, MYB transcription factor, anthocyanin

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