Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 477-486.doi: 10.16420/j.issn.0513-353x.2020-0304
• Research Papers • Previous Articles Next Articles
CHENG Xiaohua1, XU Zhiwei1,2, WANG Li1, SHI Xiaoxin1,**(), DU Guoqiang1,**(
)
Received:
2020-09-24
Online:
2021-03-25
Published:
2021-04-02
Contact:
SHI Xiaoxin,DU Guoqiang
E-mail:shixx@hebau.edu.cn;gdu@hebau.edu.cn
CLC Number:
CHENG Xiaohua, XU Zhiwei, WANG Li, SHI Xiaoxin, DU Guoqiang. Efficacy of Low Temperature and Mineral Oil Covering in Prolonging Subculture Interval During Grape Germplasm Preservation in Vitro[J]. Acta Horticulturae Sinica, 2021, 48(3): 477-486.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0304
温度/℃ Tempera- ture | 红斯 威特 Sweet Scarlet | 莫丽莎 Melissa | 皇家 夏天 Summer Royal | 克瑞森 无核 Crimson Seedless | 巨峰 Kyoho | 巨玫瑰 Jumeigui | 皇家 秋天 Autumn Royal | 火焰 无核 Flame Seedless | 夏黑 Summer Black | 粒粒 特 Lilit | 公主 Princess | 赤霞珠 Cabernet Sauvignon | 美人指 Manicure Finger | 魏可 Wink |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9 | 1 060 | 1 060 | 850 | 850 | 510 | 450 | 270 | 380 | 270 | 330 | 280 | 350 | 180 | 170 |
12 | 650 | 650 | 650 | 650 | 550 | 600 | 510 | 550 | 500 | 460 | 430 | 370 | 260 | 300 |
15 | 330 | 330 | 320 | 400 | 390 | 330 | 390 | 340 | 400 | 360 | 340 | 390 | 350 | 360 |
25 | 140 | 140 | 140 | 160 | 150 | 140 | 150 | 150 | 130 | 130 | 160 | 150 | 140 | 140 |
Table 1 Effect of preserve temperature on the maximum subculture days in grape plantlets in vitro /d
温度/℃ Tempera- ture | 红斯 威特 Sweet Scarlet | 莫丽莎 Melissa | 皇家 夏天 Summer Royal | 克瑞森 无核 Crimson Seedless | 巨峰 Kyoho | 巨玫瑰 Jumeigui | 皇家 秋天 Autumn Royal | 火焰 无核 Flame Seedless | 夏黑 Summer Black | 粒粒 特 Lilit | 公主 Princess | 赤霞珠 Cabernet Sauvignon | 美人指 Manicure Finger | 魏可 Wink |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9 | 1 060 | 1 060 | 850 | 850 | 510 | 450 | 270 | 380 | 270 | 330 | 280 | 350 | 180 | 170 |
12 | 650 | 650 | 650 | 650 | 550 | 600 | 510 | 550 | 500 | 460 | 430 | 370 | 260 | 300 |
15 | 330 | 330 | 320 | 400 | 390 | 330 | 390 | 340 | 400 | 360 | 340 | 390 | 350 | 360 |
25 | 140 | 140 | 140 | 160 | 150 | 140 | 150 | 150 | 130 | 130 | 160 | 150 | 140 | 140 |
Fig. 1 The effect of different preserve temperature on the height of plantlets in vitro in grapes Different letters in this figure represent the significance at the level of P < 0.05. The same below. The shoot apical part of some plantlets was withered,which led the plant height decreased.
Fig. 5 The status of the‘Melissa’grape plantlets that were preserved at 9 ℃ for different days(A-C)and resumed growth in routine culture condition in first generation(D-F)
保存天数 Preserved days | 红斯威特 Sweet Scarlet | 莫丽莎 Melissa | 克瑞森无核 Crimson Seedless | ||||||
---|---|---|---|---|---|---|---|---|---|
株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | 株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | 株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | |
238 | 4.38 ± 0.33 a | 3.33 ± 0.68 a | 2.05 ± 0.41 a | 8.20 ± 0.20 a | 6.17 ± 0.14 a | 1.72 ± 0.22 a | 6.75 ± 0.20 a | 6.33 ± 0.14 a | 1.72 ± 0.05 a |
600 | 3.27 ± 0.32 a | 2.87 ± 0.35 a | 0.98 ± 0.10 ab | 5.53 ± 0.57 b | 5.73 ± 0.58 ab | 1.40 ± 0.29 a | 4.42 ± 0.24 b | 5.45 ± 0.18 ab | 1.25 ± 0.20 a |
843 | 2.84 ± 0.39 a | 2.01 ± 0.44 a | 0.65 ± 0.10 b | 4.03 ± 0.44 b | 3.83 ± 0.59 b | 0.80 ± 0.03 a | 3.58 ± 0.65 b | 3.17 ± 0.49 b | 1.05 ± 0.20 a |
Table 2 The status of resumed growth of the plantlets in routine culture condition after being previously preserved at 9 ℃
保存天数 Preserved days | 红斯威特 Sweet Scarlet | 莫丽莎 Melissa | 克瑞森无核 Crimson Seedless | ||||||
---|---|---|---|---|---|---|---|---|---|
株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | 株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | 株高/cm Plantlet height | 叶片数 Leaf number | 叶面积/cm2 Leaf area | |
238 | 4.38 ± 0.33 a | 3.33 ± 0.68 a | 2.05 ± 0.41 a | 8.20 ± 0.20 a | 6.17 ± 0.14 a | 1.72 ± 0.22 a | 6.75 ± 0.20 a | 6.33 ± 0.14 a | 1.72 ± 0.05 a |
600 | 3.27 ± 0.32 a | 2.87 ± 0.35 a | 0.98 ± 0.10 ab | 5.53 ± 0.57 b | 5.73 ± 0.58 ab | 1.40 ± 0.29 a | 4.42 ± 0.24 b | 5.45 ± 0.18 ab | 1.25 ± 0.20 a |
843 | 2.84 ± 0.39 a | 2.01 ± 0.44 a | 0.65 ± 0.10 b | 4.03 ± 0.44 b | 3.83 ± 0.59 b | 0.80 ± 0.03 a | 3.58 ± 0.65 b | 3.17 ± 0.49 b | 1.05 ± 0.20 a |
处理 Treatment | 存活率/% Survival rate | 株高/cm Plantlet height | 生根率/% Rooting rate | 生根数 Number of roots | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
未加矿物油(对照) Without mineral oil adding(Control) | 23.41 b | ≥ 8.50 a | 100.00 a | 3.33 a | ||||||||||
接种后覆盖矿物油 Covering mineral oil while inoculation | 100.00 a | 2.36 b | 66.67 b | 1.11 b | ||||||||||
培养15 d后覆盖矿物油 Covering mineral oil at 15 d after inoculation | 0 b | — | 100.00 a | 3.44 a |
Table 3 Effect of mineral oil covering on the retarding growth of plantlets in vitro in‘Sweet Scarlet’grapes(Preserved for 150 d)
处理 Treatment | 存活率/% Survival rate | 株高/cm Plantlet height | 生根率/% Rooting rate | 生根数 Number of roots | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
未加矿物油(对照) Without mineral oil adding(Control) | 23.41 b | ≥ 8.50 a | 100.00 a | 3.33 a | ||||||||||
接种后覆盖矿物油 Covering mineral oil while inoculation | 100.00 a | 2.36 b | 66.67 b | 1.11 b | ||||||||||
培养15 d后覆盖矿物油 Covering mineral oil at 15 d after inoculation | 0 b | — | 100.00 a | 3.44 a |
品种 Variety | 矿物油厚度/cm Mineral oil | 120 d | 180 d | 360 d | ||||
---|---|---|---|---|---|---|---|---|
存活率/% Survival rate | 株高/cm Plantlet height | 生根率/% Rooting rate | 生根数 Number of roots | 存活率/% Survival rate | 株高/cm Plantlet height | 存活率/% Survival rate | ||
巨玫瑰 Jumeigui | 0(对照Control) | 100 | ≥ 8.50 | 100.0 | 2.87 | 0 | ||
6 | 100 | 2.18 | 99.0 | 4.24 | 100 | 2.30 | 64.0 | |
莫丽莎 Melissa | 0 | 100 | ≥ 8.50 | 100.0 | 5.60 | 0 | ||
6 | 100 | 2.14 | 60.0 | 1.32 | 100 | 2.28 | 81.1 | |
巨峰 Kyoho | 0 | 100 | ≥ 8.50 | 100.0 | 3.50 | 0 | ||
6 | 100 | 2.39 | 100.0 | 3.89 | 100 | 2.56 | 39.2 | |
夏黑 Summer Black | 0 | 100 | ≥ 8.50 | 100.0 | 3.33 | 0 | ||
6 | 100 | 1.97 | 100.0 | 1.97 | 100 | 2.33 | 74.3 | |
粒粒特 Lilit | 0 | 100 | ≥ 8.50 | 100.0 | 6.73 | 0 | ||
6 | 100 | 1.44 | 48.0 | 1.24 | 100 | 2.16 | 63.3 | |
皇家夏天 Summer Royal | 0 | 100 | ≥ 8.50 | 100.0 | 6.76 | 0 | ||
6 | 100 | 1.95 | 100.0 | 1.90 | 100 | 2.35 | 50.0 | |
皇家秋天 Autumn Royal | 0 | 100 | ≥ 8.50 | 100.0 | 4.12 | 0 | ||
6 | 100 | 1.82 | 64.0 | 3.36 | 100 | 2.25 | 68.6 | |
克瑞森无核 Crimson Seedless | 0 | 100 | ≥ 8.50 | 100.0 | 12.44 | 0 | ||
6 | 100 | 2.34 | 88.0 | 4.48 | 100 | 2.60 | 65.7 | |
赤霞珠 Cabernet Sauvignon | 0 | 100 | ≥ 8.50 | 100.0 | 5.56 | 0 | ||
6 | 100 | 1.46 | 80.0 | 1.58 | 84 | 1.98 | 75.0 |
Table 4 Effect of covering mineral oil on retarding growth of plantlets in vitro in grapes
品种 Variety | 矿物油厚度/cm Mineral oil | 120 d | 180 d | 360 d | ||||
---|---|---|---|---|---|---|---|---|
存活率/% Survival rate | 株高/cm Plantlet height | 生根率/% Rooting rate | 生根数 Number of roots | 存活率/% Survival rate | 株高/cm Plantlet height | 存活率/% Survival rate | ||
巨玫瑰 Jumeigui | 0(对照Control) | 100 | ≥ 8.50 | 100.0 | 2.87 | 0 | ||
6 | 100 | 2.18 | 99.0 | 4.24 | 100 | 2.30 | 64.0 | |
莫丽莎 Melissa | 0 | 100 | ≥ 8.50 | 100.0 | 5.60 | 0 | ||
6 | 100 | 2.14 | 60.0 | 1.32 | 100 | 2.28 | 81.1 | |
巨峰 Kyoho | 0 | 100 | ≥ 8.50 | 100.0 | 3.50 | 0 | ||
6 | 100 | 2.39 | 100.0 | 3.89 | 100 | 2.56 | 39.2 | |
夏黑 Summer Black | 0 | 100 | ≥ 8.50 | 100.0 | 3.33 | 0 | ||
6 | 100 | 1.97 | 100.0 | 1.97 | 100 | 2.33 | 74.3 | |
粒粒特 Lilit | 0 | 100 | ≥ 8.50 | 100.0 | 6.73 | 0 | ||
6 | 100 | 1.44 | 48.0 | 1.24 | 100 | 2.16 | 63.3 | |
皇家夏天 Summer Royal | 0 | 100 | ≥ 8.50 | 100.0 | 6.76 | 0 | ||
6 | 100 | 1.95 | 100.0 | 1.90 | 100 | 2.35 | 50.0 | |
皇家秋天 Autumn Royal | 0 | 100 | ≥ 8.50 | 100.0 | 4.12 | 0 | ||
6 | 100 | 1.82 | 64.0 | 3.36 | 100 | 2.25 | 68.6 | |
克瑞森无核 Crimson Seedless | 0 | 100 | ≥ 8.50 | 100.0 | 12.44 | 0 | ||
6 | 100 | 2.34 | 88.0 | 4.48 | 100 | 2.60 | 65.7 | |
赤霞珠 Cabernet Sauvignon | 0 | 100 | ≥ 8.50 | 100.0 | 5.56 | 0 | ||
6 | 100 | 1.46 | 80.0 | 1.58 | 84 | 1.98 | 75.0 |
[1] | Ahmed M, Anjum M A, Shah A H, Hamid A. 2010. In vitro preservation of Pyrus germplasm with minimal growth using different temperature regimes. Pakistan Journal of Botany, 42 (3),1639-1650. |
[2] |
Bridgen M P, Staby G L. 1981. Low pressure and low oxygen storage of Nicotiana tabacum and Chrysanthemum × morifolium tissue cultures. Plant Science Letters, 22 (2):177-186.
doi: 10.1016/0304-4211(81)90140-1 URL |
[3] |
Caplin S M. 1959. Mineral oil overlay for conservation of plant tissue cultures. American Journal of Botany, 46 (5):324-329.
doi: 10.1002/ajb2.1959.46.issue-5 URL |
[4] | Engelmann F. 1997. In vitro conservation methods. Biotechnology in Agriculture Series,(19):119-162. |
[5] | Hassan N S. 2004. Storage of in vitro banana shoot cultures at low temperature or under mineral oil layer. International Journal of Agriculture & Biology, 6 (2):303-306. |
[6] | Liu Yue-xue, Liu Xiao-jun, Wang Jia-fu, Lin Shun-quan. 2004. Effects of low temperature and another condition on Eriobotrya japonica germplasm preservation in vitro. Journal of Plant Resources and Environment,(1):28-31. (in Chinese) |
刘月学, 刘小军, 王家福, 林顺权. 2004. 低温等因素对枇杷种质离体保存的影响. 植物资源与环境学报,(1):28-31. | |
[7] | Lonardo C S D. 2013. In vitro conservation of chestnut( Castanea sativa)by slow growth. In Vitro Cellular & Developmental Biology-Plant, 49:605-610. |
[8] |
Moriguchi T, Kozaki I, Matsuta N, Yamaki S. 1988. Plant regeneration from grape callus stored under a combination of low temperature and silicone treatment. Plant Cell Tissue and Organ Culture, 15 (1):67-71.
doi: 10.1007/BF00039890 URL |
[9] | Niu Ai-guo, Zhang Kai-chun, Zhang Xiao-ming, Yan Guo-hua, Li Wen-sheng, Jiang Li-jie. 2005. Preservation of in vitro grown shoot of cherry germplasm. Acta Horticulturae Sinica, 32 (2):298-300. (in Chinese) |
牛爱国, 张开春, 张晓明, 闫国华, 李文生, 姜立杰. 2005. 樱桃种质资源试管苗保存方法. 园艺学报, 32 (2):298-300. | |
[10] |
Ozudogru E. A, Benelli C, Dradi G, Lambardi M. 2017. Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock‘Gisela® 5’. Acta Physiologiae Plantarum, 39 (4):94.
doi: 10.1007/s11738-017-2372-2 URL |
[11] | Pan X J, Zhang W E, Li X. 2014. In vitro conservation of native Chinese wild grape( Vitis heyneana Roem. & Schult)by slow growth culture. Vitis, 53 (4):207-214. |
[12] |
Sharma N, Satsangi R, Pandey R, Singh R, Kaushik N, Tyagi R K. 2012. In vitro conservation of Bacopa monnieri(L.)using mineral oil. Plant Cell Tissue and Organ Culture, 111 (3):291-301.
doi: 10.1007/s11240-012-0194-x URL |
[13] | Sota V, Kongjika E. 2014. Slow growth in vitro conservation of Zizyphus jujuba Mill. Agriculture & Forestry, 60 (1):27-37. |
[14] | Wang Chen. 2008. Studies on growth characteristics and rhythm regulation of apple plantlets repeatedly subcultured in vitro[M. D. Dissertation]. Baoding:Agricultural University of Hebei. (in Chinese) |
王晨. 2008. 苹果组培苗多代保存过程中生长特性及生长节奏调控技术研究[硕士论文]. 保定:河北农业大学. | |
[15] | Wu Yong-jie, Li Yu-sheng, Wu Ya-qin, Chen Long, Cheng He-he, Zhao Yan-hua. 2019. Advances in conservation of plant germplasm resources. Hebei Fruits,(1):1-3. (in Chinese) |
吴永杰, 李玉生, 吴雅琴, 陈龙, 程和禾, 赵艳华. 2019. 植物种质资源保存研究进展. 河北果树,(1):1-3. | |
[16] | Xu Gang-biao. 2000. Plant germplasm conservation in vitro. Journal of Central South Forestry University,(4):81-87. (in Chinese) |
徐刚标. 2000. 植物种质资源离体保存研究进展. 中南林学院学报,(4):81-87. | |
[17] | Xu Zhi-wei, Yang Li-li, Du Guo-qiang, Shi Xiao-xin. 2014. Efficacy of osmotic pressure and plant growth regulators in media on conservation of grape plantlets in vitro. Molecular Plant Breeding, 12 (4):720-725. (in Chinese) |
徐志微, 杨丽丽, 杜国强, 师校欣. 2014. 培养基渗透压和生长调节剂对葡萄种质离体保存的效应. 分子植物育种, 12 (4):720-725. |
[1] | WANG Xiaochen, NIE Ziye, LIU Xianju, DUAN Wei, FAN Peige, and LIANG Zhenchang, . Effects of Abscisic Acid on Monoterpene Synthesis in‘Jingxiangyu’Grape Berries [J]. Acta Horticulturae Sinica, 2023, 50(2): 237-249. |
[2] | LIN Haijiao, LIANG Yuchen, LI Ling, MA Jun, ZHANG Lu, LAN Zhenying, YUAN Zening. Exploration and Regulation Network Analysis of CBF Pathway Related Cold Tolerance Genes in Lavandula angustifolia [J]. Acta Horticulturae Sinica, 2023, 50(1): 131-144. |
[3] | WANG Baoliang, LIU Fengzhi, JI Xiaohao, WANG Xiaodi, SHI Xiangbin, ZHANG Yican, LI Peng, and WANG Haibo. A New Early Ripening Grape Cultivar‘Huapu Zaoyu’for Table [J]. Acta Horticulturae Sinica, 2022, 49(S2): 33-34. |
[4] | WANG Baoliang, WANG Haibo, JI Xiaohao, WANG Xiaodi, SHI Xiangbin, WANG Zhiqiang, WANG Xiaolong, and LIU Fengzhi. A New Middle Ripening Grape Cultivar‘Huapu Huangyu’for Table [J]. Acta Horticulturae Sinica, 2022, 49(S2): 35-36. |
[5] | A Late-maturing Seedless Grape Cultivar‘Zilongzhu’. A Late-maturing Seedless Grape Cultivar‘Zilongzhu’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 37-38. |
[6] | SHI Xiaoxin, DU Guoqiang, YANG Lili, QIAO Yuelian, HUANG Chengli, WANG Suyue, ZHAO Yuexin, WEI Xiaohui, WANG Li, and QI Xiangli. A Late-ripening Seedless Grape Cultivar‘Hongfeng Wuhe’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 39-40. |
[7] | WU Yueyan, CHEN Tianchi, WANG Liru, HAN Shanqi, and FU Tao. A New Table Grape Cultivar‘Yongzaohong’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 41-42. |
[8] | HUANG Shuping, TAN Jie, Chen Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 6’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 101-102. |
[9] | WANG Xiaoyue, YAN Ailing, ZHANG Guojun, WANG Huiling, REN Jiancheng, LIU Zhenhua, SUN Lei, and XU Haiying, . A New Grape Cultivar‘Ruidu Wanhong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 29-30. |
[10] | NIE Xinmiao, LUAN Heng, FENG Gaili, WANG Chao, LI Yan, WEI Min. Effects of Silicon Nutrition and Grafting Rootstocks on Chilling Tolerance of Cucumber Seedlings [J]. Acta Horticulturae Sinica, 2022, 49(8): 1795-1804. |
[11] | WANG Yongjian, KONG Junhua, FAN Peige, LIANG Zhenchang, JIN Xiuliang, LIU Buchun, DAI Zhanwu. Grape Phenome High-throughput Acquisition and Analysis Methods:A Review [J]. Acta Horticulturae Sinica, 2022, 49(8): 1815-1832. |
[12] | WEI Xiaoyu, WANG Yuejin. Correlation Between Anatomical Structure and Resistance to Powdery Mildew in Chinese Wild Vitis Species [J]. Acta Horticulturae Sinica, 2022, 49(6): 1200-1212. |
[13] | LIU Zhongjie, ZHENG Ting, ZHAO Fanggui, FU Weihong, ZHUGE Yaxian, ZHANG Zhichang, FANG Jinggui. Resistance Difference and Physiological Response Mechanism of Grape Rootstocks to Osmotic Stress [J]. Acta Horticulturae Sinica, 2022, 49(5): 984-994. |
[14] | LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang, WANG Li. Genome-wide Identification of Grape GRF Family and Expression Analysis [J]. Acta Horticulturae Sinica, 2022, 49(5): 995-1007. |
[15] | LI Shasha, YU Saisai, FU Yuheng, LUO Qiangwei, XU Yan, WANG Yuejin. The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes [J]. Acta Horticulturae Sinica, 2022, 49(4): 723-738. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2012 Acta Horticulturae Sinica 京ICP备10030308号-2 国际联网备案号 11010802023439
Tel: 010-82109523 E-Mail: yuanyixuebao@126.com
Support by: Beijing Magtech Co.Ltd