Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology

doi:10.16420/j.issn.0513-353x.2016-0519

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (2): 215-222.doi: 10.16420/j.issn.0513-353x.2016-0519

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Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology

LIU Yuanxia¹，LAN Jinhao²，BAI Suhua³，SUN Xiaohong³，LIU Chunxiao¹，ZHANG Yugang¹，and DAI Hongyi^1,^*

¹Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants，College of Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109，China；²College of Agronomy and Crop Protection，Qingdao Agricultural University，Qingdao，Shandong 266109，China；³College of Life Sciences，Qingdao Agricultural University，Qingdao，Shandong 266109，China

Online:2017-02-25 Published:2017-02-25

Abstract

Abstract:

In present study，10 SNP and 10 InDel markers associated with Glomerella leaf spot（GLS） resistance in the region ofR_gls，on apple linkage group（LG）15，in a region of 500 kb between S0304673 and S0405127 markers，obtained by using the whole genome sequencing technology were tested in the population of 207 F₁individuals derived from a cross between‘Golden Delicious’and‘Fuji’apples by High Resolution Melting（HRM）. Two SNP and 4 InDel markers tightly linked to the R_gls locus were screened. Through analyzing the genotypes and phenotypes of the recombinant individuals，the R_glslocus was fine mapped within a distance of 100 kb between InDel4199 and SNP4299 markers on the‘Golden Delicious’genome sequence and was co-segregated with InDel4227 and InDel4254 markers. Forty genes were predicted within the region of 4.1–4.3 Mb flanked the R_glslocus，including cellular component，molecular function and biological process in a total of 19 GO classifications.

Key words: apple, HRM, Glomerella leaf spot, SNP marker, InDel marker, fine mapping

CLC Number:

S 661.1

LIU Yuanxia1，LAN Jinhao2，BAI Suhua3，SUN Xiaohong3，LIU Chunxiao1，ZHANG Yugang1，and DAI Hongyi1,*. Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology[J]. ACTA HORTICULTURAE SINICA, 2017, 44(2): 215-222.

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Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology

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