Identification of CaTPS Gene Family and Expression Analysis of CaTPS1 in Hot Pepper

doi:10.16420/j.issn.0513-353x.2016-0207

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (8): 1504-1512.doi: 10.16420/j.issn.0513-353x.2016-0207

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Identification of CaTPS Gene Family and Expression Analysis of CaTPS1 in Hot Pepper

WEI Bing-qiang^1,2，WANG Lan-lan²，ZHANG Ru²，CHEN Ling-zhi²，ZHANG Shao-li²，and ZHANG Jian-nong^1,*

（¹College of Horticulture，Gansu Agricultural University，Lanzhou 730070，China；²Institute of Vegetable，Gansu Academy of Agricultural Sciences，Lanzhou 730070，China）

Online:2016-08-25 Published:2016-08-25

Abstract

Abstract:

Trehalose and the precursor trehalose-6-phosphate participate in responding to abiotic stress as the osmotic regulator and the signaling molecule. Trehalose-6-phosphate synthase（TPS）is one of the most important enzyme among the synthesis mechanism of trehalose-6-phosphate，and plays an important role in the metabolic pathway of trehalose. The over expression of TPS gene can improve crop’s tolerance to abiotic stress. Based on pepper genome database，11 genes of CaTPS family were identified by bioinformatics methods. Besides CaTPS3 contains one TPS and two TPP domains，the rest of 10 CaTPS family genes contain one TPS and one TPP domain. All 11 CaTPS family genes were classified into two classes according to their gene structure and phenogenesis. CaTPS1，CaTPS2 and CaTPS3 are grouped to ClassⅠ. CaTPS1 and CaTPS2 respectively contains 16 introns and 5 motifs from motif 1 to motif 5，while CaTPS3 contains 13 introns，one motif 3 and two motif 4. The rest eight genes from CaTPS4 to CaTPS11

respectively contains two introns and six motifs from motif 1 to motif 6，and they are grouped to ClassⅡ. The expression of tissue specificity and response to low temperature stress of CaTPS1 were analyzed in hot pepper using quantitative real-time PCR. The results showed that the expressive quantity of CaTPS1 gene in leaves were five to six times than that in stems and roots，this indicated that the expression of CaTPS1 appeared obvious tissue specificity in hot pepper. After treated with low temperature，the expressing peak of CaTPS1 in roots and stems appeared earlier than that in leaves，which suggested that the responding times to abiotic stress of CaTPS1 were no accordance between different tissues.

Key words: hot pepper, trehalose-6-phosphate synthase gene, genomic identification, gene expression

CLC Number:

S 641.3

WEI Bing-qiang，WANG Lan-lan，ZHANG Ru，CHEN Ling-zhi，ZHANG Shao-li，and ZHANG Jian-nong. Identification of CaTPS Gene Family and Expression Analysis of CaTPS1 in Hot Pepper[J]. ACTA HORTICULTURAE SINICA, 2016, 43(8): 1504-1512.

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Identification of CaTPS Gene Family and Expression Analysis of CaTPS1 in Hot Pepper

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