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园艺学报 ›› 2009, Vol. 36 ›› Issue (11): 1581-1588.

• 果树 • 上一篇    下一篇

MdMYB1转录因子调控红星苹果果实着色的计算机模拟分析及表达验证

张华磊1,2;毛柯1,2;刘志2,3;谢兴斌1,2;冯晓明1,2;郝玉金1,2*   

  1. (1山东农业大学作物生物学国家重点实验室, 山东泰安271018; 2山东农业大学园艺科学与工程学院, 山东泰安271018; 3辽宁省果树科学研究所, 辽宁熊岳115009)
  • 收稿日期:2009-07-03 修回日期:2009-09-21 出版日期:2009-11-25 发布日期:2009-11-25
  • 通讯作者: 郝玉金

In silico Analysis and Expression Confirmation of the Regulation of FruitColoration by Transcriptional Factor MdMBY1 in Delicious Apple

ZHANG Hua-lei1,2;MAO Ke1,2;LIU Zhi2,3;XIE Xing-bin1,2;FENG Xiao-ming1,2; HAO Yu-jin1,2*   

  1. (1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, China; 2College of Horticulture Science and Engineering, Shandong AgriculturalUniversity, Taipan, Shandong 271018, China; 3 Liaoning Institute of Pomology, Xiongyue, Liaoning 115009, China)
  • Received:2009-07-03 Revised:2009-09-21 Online:2009-11-25 Published:2009-11-25
  • Contact: HAO Yu-jin

摘要: 从‘红星’苹果(Malus domestica‘Delicious’) 果实中克隆获得了MdMYB1基因, 通过生物信息学分析( in silico analysis) 和活体细胞融合蛋白荧光观察, 确定其编码蛋白定位于细胞核。同时, 克隆了MdDFRMdUFGT基因的启动子, 模拟分析表明2个基因的启动子序列中含有MYB结合的顺式作用元件。为了探讨MdMYB1转录因子是否调控MdDFRMdUFGT基因的表达, 对不同光照条件下果皮花青素含量和基因表达进行了检测, 结果表明光照能够诱导花青素积累, 并迅速启动3个基因表达, 且3个基因表现出高度相似的表达模式, 表明MdMYB1转录因子可能直接调控MdDFRMdUFGT基因的表达。

关键词: 苹果, 果实, 着色基因, 光诱导, 表达分析

Abstract: In this study, MdMYB1 was cloned from Delicious apple. Itwas found that MdMYB1 protein was localized in nucleus indicated by in silico predication and GFP fusion protein fluorescence observation. In parallel, the promoter regions of MdDFT and MdUFGT were isolated and analyzed in silico. The result showed that both promoters contained cis-elements characterized as MYB binding sites. Furthermore, to examine if MdMYB1 triggers the expression of MdDFR and MdUFGT during fruit coloration, anthocyanin content, expression levels of MdMYB1, MdDFR and MdUFGT were investigated. The results demonstrated that light exposure markedly induced anthocyanin accumulation in fruit peel, along with a rapid initiation of gene expression. Interestingly, three genes were expressed in a highly similar pattern. In one word, our findings suggest that transcription factor MdMYB1 may directly regulate the expression of MdDFR and MdUFGT genes.

Key words: apple, fruit, coloration gene, light induction, expression analysis

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