调控番茄果实柠檬酸含量的基因定位

doi:10.16420/j.issn.0513-353x.2023-0047

摘要/Abstract

摘要：

为了挖掘调控番茄果实柠檬酸含量的关键基因，以栽培番茄（Solanum lycopersicum ‘Moneymaker’）和野生醋栗番茄（Solanum pimpinellifolium‘PI365967’）为亲本构建的重组自交系群体为材料，利用混池重测序技术挖掘到6个控制柠檬酸含量的QTL。其中位于6号染色体末端的主效QTL qCA6.1表型贡献率高达19.28%。为对该位点进行定位，从重组自交系群体中挑选出2份柠檬酸含量差异较大，且只在主效位点存在基因型差异的株系作为亲本，构建BC₂F₂定位群体，通过交换单株后代鉴定，将定位区间缩小到342 kb区间内，并开发了连锁分子标记，为后续克隆基因奠定了基础，也为改良番茄果实柠檬酸含量提供了重要选择标记。

关键词: 番茄, 风味, 柠檬酸, 重组自交系, QTL

Abstract:

The content of citric acid in tomato fruit is one of the key factors affecting the flavor. Due to the citric acid content is greatly affected by the environment few genes related with citric acid content have been cloned. In this study，six QTLs，which control the content of citric acid，were identified using a recombinant inbred lines generated by crossing modern cultivated tomato‘Moneymaker’and S. pimpinellifolium‘PI365967’. Among them，q-CA6.1，a major QTL located at the terminal of chromosome 6，contributed 19.28% variation of citric acid content. To map qCA6.1 gene a BC₂F₂ mapping population was obtained by crossing two RILs with significant difference in citric acid content and with similar genotype and the difference only around the qCA6.1 region. The qCA6.1 gene were narrowed into 342 kb region including 47 gene. The development of linked molecular markers provides technical support for improving citric acid content in tomato breeding.

Key words: tomato, flavor, citric acid, recombinant inbred lines, QTL

田茂森, 周震, 王海敬, 崔霞, 孙帅. 调控番茄果实柠檬酸含量的基因定位[J]. 园艺学报, 2024, 51(1): 67-76.

TIAN Maosen, ZHOU Zhen, WANG Haijing, CUI Xia, SUN Shuai. Mapping of Gene Regulating Citric Acid Content in Tomato Fruit[J]. Acta Horticulturae Sinica, 2024, 51(1): 67-76.

图/表 9

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标记名称	正向引物（5′-3′）	反向引物（5′-3′）
Primer name	Forward primer	Reverse primer
M1	AAGCCATGCTACCATTGCTAGCT	AACCCATGCTTCAATGGTGAG
M2	TCTTGGTTACCGAGAGTTATAGCTTCTACG	ACTCGATCAACTGTGCTACATTAGATG
M3	ACCTGTCCAGAAAAACTGAAC	TCTGTTACTTGAGCTGAGC
M4	TTCTTCTCTCGTTCTCCTCC	TCGAGATCATCAATTTCCAAGGTCATTGGTG
M5	TGTGTAAGCTTGGTCGATG	ACTTAACGTGCTCGATGAAG
M6	TGCTTGAAGTCTCCTGATCATCC	TCCAACTATTGCCCATGATTTCCTG
M7	TTCTTCTCTCGTTCTCCTCC	TCGAGATCATCAATTTCCAAGGTCATTGGTG

标记名称	正向引物（5′-3′）	反向引物（5′-3′）
Primer name	Forward primer	Reverse primer
M1	AAGCCATGCTACCATTGCTAGCT	AACCCATGCTTCAATGGTGAG
M2	TCTTGGTTACCGAGAGTTATAGCTTCTACG	ACTCGATCAACTGTGCTACATTAGATG
M3	ACCTGTCCAGAAAAACTGAAC	TCTGTTACTTGAGCTGAGC
M4	TTCTTCTCTCGTTCTCCTCC	TCGAGATCATCAATTTCCAAGGTCATTGGTG
M5	TGTGTAAGCTTGGTCGATG	ACTTAACGTGCTCGATGAAG
M6	TGCTTGAAGTCTCCTGATCATCC	TCCAACTATTGCCCATGATTTCCTG
M7	TTCTTCTCTCGTTCTCCTCC	TCGAGATCATCAATTTCCAAGGTCATTGGTG

位点	分子标记	染色体	物理位置/kb	表型变异贡献率/%
QTL	Molecular marker	Chromosome	Postion on SL2.50	PVE
qCA2.1	M8	2	51 038 ~ 51 377	6.11
qCA6.1	M9	6	41 515 ~ 45 404	19.28
qCA8.1	M10	8	2 079 ~ 2 746	1.51
qCA10.1	M11	10	199 ~ 499	2.08
qCA12.1	M12	12	5 236 ~ 6 213	1.70
qCA12.2	M13	12	38 976 ~ 39 425	2.44

位点	分子标记	染色体	物理位置/kb	表型变异贡献率/%
QTL	Molecular marker	Chromosome	Postion on SL2.50	PVE
qCA2.1	M8	2	51 038 ~ 51 377	6.11
qCA6.1	M9	6	41 515 ~ 45 404	19.28
qCA8.1	M10	8	2 079 ~ 2 746	1.51
qCA10.1	M11	10	199 ~ 499	2.08
qCA12.1	M12	12	5 236 ~ 6 213	1.70
qCA12.2	M13	12	38 976 ~ 39 425	2.44

单株编号 Plant number	分子标记Molecular marker						柠檬酸含量/（mg · g^-1FW） Citric acid content
单株编号 Plant number	M1	M2	M3	M4	M5	M6	柠檬酸含量/（mg · g^-1FW） Citric acid content
21N16-204	M	M	M	M	M	M	9.03 ± 1.26
21N16-209	P	P	P	P	P	P	18.55 ± 1.69
21N16-3	M	P	P	P	P	P	16.36 ± 1.80
21N16-14	M	M	M	M	P	P	8.68 ± 1.65
21N16-17	M	M	M	M	P	P	10.51 ± 0.28
21N16-2	P	P	M	M	M	M	8.77 ± 0.87