图1 菌株XDY66对灰葡萄孢，辣椒疫霉和番茄枯萎病菌的拮抗作用

Fig. 1 Antagonism of strain XDY66 against Botrytis cinerea，Phytophthora capsici and Fusarium oxysporum

图2 灰葡萄孢在添加XDY66菌液的PDA液体培养基中菌丝畸形、顶部肿大（箭头）

Fig. 2 Botrytis cinerea’s mycelia deformity and top enlargement in PDA liquid medium supplemented with XDY66（arrow）

图3 接种灰葡萄孢（Botrytis cinerea）和菌株XDY66处理的番茄果实的发病情况

Fig. 3 The incidence of tomato fruits inoculated with Botrytis cinerea and strain XDY66

图4 菌株XDY66的革兰氏染色（A）和芽孢染色（B）

Fig. 4 Gram staining（A）and spore staining（B）of strain XDY66

图5 菌株XDY66基于16S rDNA的系统发育树

Fig. 5 Phylogenetic tree of strain XDY66 and similar strains based on 16S rDNA

图6 Bacillus velezensis XDY66的基因组圈图 从外到内第1和第4圈为正链、负链上的CDS，不同颜色表示不同的COG功能分类；第2和第3圈分别为正链、负链上的CDS、tRNA、rRNA；第5圈为GC含量，向外或向内分别表示该区域GC含量高于或低于全基因组平均含量，峰值越高表示与平均含量差值越大；第6圈为GC-Skew值，具体算法为G-C/G+C，可以辅助判断前导链和后滞链。

Fig. 6 Circular genome map of Bacillus velezensis XDY66 The first and fourth circles from the outside to the inside are CDS on the positive and negative chains，and different colors represent different COG functional classifications；The second and third circles are CDS，tRNA and rRNA on the positive and negative chains，respectively. The fifth circle is the GC content. The outward or inward direction indicates that the GC content in this region is higher or lower than the average content of the whole genome，and the higher the peak value indicates the greater the difference from the average content. The sixth circle is the GC-Skew value，and the specific algorithm is G-C/G+C，which can assist in judging the leading chain and the lagging chain.

图7 Bacillus velezensis XDY66的基因组同源聚类基因簇（COG）分析 A ~ Z 同图6。

Fig. 7 Homologous cluster（COG）analysis of Bacillus velezensis XDY66 A-Z are shown in Fig. 6.

图8 Bacillus velezensis XDY66与Bacillus velezensis FZB42、SQR9的基因组共线性分析 颜色相同的方框表示共线区域，彩色线表示重排。

Fig. 8 Genome comparison of Bacillus velezensis XDY66 with B. velezensis FZB42 and B. velezensis SQR9 Boxes with the same color are represented the syntenic regions，rearrangements are shown by colored lines.

图9 Bacillus velezensis XDY66与B. velezensis FZB42、B. velezensis SQR9的基因组共有、特有的同源CDS基因数韦恩图

Fig. 9 Venn figure，number of common and endemic homologous CDS genes of Bacillus velezensis XDY66，B. velezensis FZB42 and B. velezensis SQR9

图10 Bacillus velezensis XDY66、FZB42和SQR9的次生代谢产物基因簇共线性分析 1 ~ 14分别是相应的次级代谢产物基因簇。1：表面活性素；2：偶氮唑肽；3：聚酮合酶相关；4：萜烯；5：Ⅱ类羊毛硫肽；6：大环内酰亚胺H；7：芽孢杆菌烯；8：芬芥素；9：萜烯；10：Ⅲ型聚酮化合物合酶；11：达菲菌素；12：杆菌素；13：溶杆菌素；14：萜烯。

Fig. 10 Collinearity analysis of secondary metabolite gene clusters of Bacillus velezensis XDY66，FZB42 and SQR9 Colinear regions are connected by black lines 1-14 are the corresponding secondary metabolite gene clusters. 1：Surfactin；2：LAP；3：Polyketide Synthase-Like；4：Terpene；5：Lanthipeptide-class-ii；6：Macrolactin H；7：Bacillaene；8：Fengycin；9：Terpene；10：Type III Polyketide Synthases；11：Difficidin；12：Bacillibactin；13：Bacilysin；14：Terpene.