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园艺学报 ›› 2021, Vol. 48 ›› Issue (9): 1680-1694.doi: 10.16420/j.issn.0513-353x.2021- 0219

• 研究论文 • 上一篇    下一篇

低温胁迫下枇杷不同发育阶段的花果转录组比较分析

徐红霞1, 周慧芬2, 李晓颖1, 姜路花3, 陈俊伟1,*()   

  1. 1浙江省农业科学院园艺研究所,杭州 310021
    2浙江省农业技术推广中心,杭州 310020
    3淳安县农业局果树技术推广站,浙江淳安 311700
  • 收稿日期:2021-05-24 修回日期:2021-08-25 出版日期:2021-09-25 发布日期:2021-09-30
  • 通讯作者: 陈俊伟 E-mail:chenjunwei@zaas.ac.cn
  • 基金资助:
    浙江省“三农六方”科技协作计划项目(2020SNLF025);国家重点研发计划项目(2020YFD1000103);浙江省重点研发计划项目(2018C02011);浙江省农业科学院地方科技合作项目(CA202009)

Comparative Transcriptome Analysis of Different Developmental Stages of Flowers and Fruits in Loquat Under Low Temperature Stress

XU Hongxia1, ZHOU Huifen2, LI Xiaoying1, JIANG Luhua3, CHEN Junwei1,*()   

  1. 1Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
    2Zhejaing Agricultural Technology Extension Center,Hangzhou 310020,China
    3Fruit Tree Technology Extension Station of Chun’an Agricultural Bureau,Chun’an,Zhejiang 311700,China
  • Received:2021-05-24 Revised:2021-08-25 Online:2021-09-25 Published:2021-09-30
  • Contact: CHEN Junwei E-mail:chenjunwei@zaas.ac.cn

摘要:

研究比较了枇杷(Eriobotrya japonica Lindl.)不同发育阶段花果的低温响应机制。以花蕾、完全开放的花和花后2周左右的幼果为试验材料,-3 ℃低温处理12 h,以未经低温处理样品为对照,测定生理生化指标并进行转录组测序分析。低温胁迫导致细胞膜破坏,超氧阴离子产生速率、丙二醛和脯氨酸含量、抗氧化酶活性明显升高。总体上抗低温能力为花蕾 > 花 > 幼果。转录组测序分析共获得6 987个差异表达基因(DEG),对这些基因进行通路注释分析,发现大量与低温胁迫相关的代谢途径,其中碳水化合物代谢中的糖酵解/糖异生、乙醛酸和二羧酸代谢及磷酸肌醇代谢途径,氨基酸代谢中的色氨酸代谢和酪氨酸代谢途径,酯类代谢中的甘油磷脂代谢、α-亚麻酸代谢和甘油酯代谢途径,次生代谢中的异喹啉生物碱生物合成途径以及能量代谢中的硫代谢途径在3个花果发育时期低温与对照的比较组中显著富集,说明这几个途径都是枇杷花果响应低温胁迫的重要代谢途径。“苯丙烷类生物合成”途径在前两个时期比较组中富集水平较高,氨基酸代谢相关途径在幼果比较组中富集更多。另外,从低温处理相关差异表达基因中筛选到了53个AP2-EREBP基因,14个WRKY基因和15个NAC基因,并对其中12个低温响应相关转录因子基因进行了qRT-PCR表达分析,进一步证实了转录组数据的准确性。

关键词: 枇杷, 花, 果实, 发育阶段, 低温, 转录组, 差异基因, 转录因子

Abstract:

The study compared the low temperature response mechanism of loquat(Eriobotrya japonica Lindl.)flowers and fruits at different developmental stages. Flower buds,fully open flowers and young fruits were used as the experimental materials,treated at-3 ℃ for 12 h,and samples without low temperature treatment were used as controls. The relevant physiological and biochemical indexes,as well as transcriptome were determined both in control and treated samples. Low temperature stress led to the destruction of cell membranes,the generation rate of superoxide anion,the content of malondialdehyde and proline,and the activity of antioxidant enzymes were dramatically increased. In general,the low temperature resistance ability was flower bud > flower > young fruit. Transcriptome sequencing analysis obtained a total of 6 987 differentially expressed genes(DEG). A large number of metabolic pathways related to low temperature stress were found,including glycolysis/gluconeogenesis,glyoxylic acid and dicarboxylic acid metabolism and phosphoinositol metabolism pathways in carbohydrate metabolism,tryptophan metabolism and tyrosine metabolism pathways in amino acid metabolism,glycerol phospholipid metabolism,α-linolenic acid metabolism and glyceride metabolism pathways in ester metabolism,isoquinoline alkaloid biosynthesis pathway in secondary metabolism,as well as thiometabolism pathway in energy metabolism,were significantly enriched in all comparison groups of low temperature and control at three flower and fruit development stages,which indicated that these pathways were important metabolic pathways in response to low temperature stress in loquat. The phenylpropanoid biosynthesis pathway was significantly enriched in both flower bud and flower comparison groups,and more amino acid metabolism pathways were enriched in young fruit comparison group after low temperature treatment. In addition,53 AP2-EREBP genes,14 WRKY genes and 15 NAC genes were screened from the differentially expressed genes related to low temperature treatment,and 12 low temperature response-related transcription factor genes were analyzed by qRT-PCR,which further confirmed the accuracy of transcriptome data.

Key words: Eriobotrya japonica, flower, fruit, development stage, low temperature, transcriptome, differentially expressed gene, transcription factor

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